Post on 22-Dec-2015
transcript
Biomedical Research Retreat 2006 1
Biomedical Engineering Research at UNL Opportunities
Contacts:
Namas Chandra, Ph.D. P.E.Associate Dean-ResearchCollege of Engineering(402) 472-8310nchandra2@unl.edu
Becky DoleTechnical Assistant(402) 472-5600bdole2@unl.edu
Biomedical Research Retreat 2006 2
What is Biomedical Engineering?
Computations
Mathematics
ChemistryPhysics
Biology
Medicine
Human Behavior
Health
Novel• Biomaterials• Implants• Devices• Instruments• Informatics
• Better health
• Improved diagnostics
• Longer life
• Faster rehabilitation
Biomedical Research Retreat 2006 3
Areas of Expertise
• Tissue Mechanics (engineering)
– Mechanical, Engineering Mechanics, Chemical, and Biomolecular
• Medical Devices (surgical or otherwise)
– Mechanical, Engineering Mechanics, Industrial
• Biomechanics/Rehabilitation
– Mechanical, Engineering Mechanics, Industrial
• Image Analysis/Pattern Recognition
– Computer Science and Engineering
Biomedical Research Retreat 2006 4
Mechanical Devices
UNL: Carl NelsonUNMC: T. Hejkal
Infant Surgical TableGoal: Allow infant retinal surgery to be performed while seated with full rotation and tilt controls
Solution: Attachment for full-size surgical table; novel anticrimp attachments for hoses; adjustable safety-lock clamp
Goal: Eliminate frequent tool changes in MIS that waste valuable OR time and cause unnecessary trauma to patients
Solution: Multifunction tool; fuzzy logic guides preoperative tool setup
Multifunction Tool forMinimally Invasive Surgery (MIS)
UNL: Carl NelsonUNMC: D. Oleynikov
Biomedical Research Retreat 2006 5
Tissue Mechanics and New Ideas
• Make surgery easier, more effective, and more accessible
– Tool tracking in minimally invasive surgery
– Modular surgical robots
– Custom tools for tasks that are repetitive and mechanically difficult/time consuming
• Tissue Mechanics– Obtain mechanical properties
– Concerned with shear behavior, not just axial stress-strain
– Developing a very small measurement fixture
Test Set-up
UNL: Carl NelsonUNMC: J. Hammel
6
Miniature In Vivo Robots for Surgery
• Better– Miniaturized robots function entirely within
the body– Access to more surgical sites and organs– Improved visualization– Reduced incisions
• Faster– Limited set-up time– Additional tools can be added or removed quickly
• Cheaper– Mass produced– Nor hardware infrastructure (laptop enabled)– Disposable
UNL: Shane FarritorUNMC: Dmitry Oleynikov
Biomedical Research Retreat 2006 7
In Vivo Robots-Details
Mobile Camera
Pan/Tilt Camera
Biopsy
Biomedical Research Retreat 2006 8
Porcine In Vivo Mobility
Biomedical Research Retreat 2006 9
Worldwide Abundant Recombinant Hemophilia Therapy Made in the Milk of Transgenic Pigs:
Pharmacokinetic Properties for Intravenous and Oral Delivery Evaluated in Hemophilic Animals
Investigating Team
William H. Velander, Kevin E. Van Cott, Todd Swanson Dept of Chemical and Biomolecular EngineeringUniversity of Nebraska-Lincoln, Lincoln, NE
Stephan Butler and Julian Cooper, Progenetics LLC, Pembroke, VA
Paul E. Monahan, Gene Therapy CenterUniversity of North Carolina at Chapel Hill, NC
Oral Alpan and Polly Matzinger,NIH/NIAID, Bethesda Maryland
1987-------------1998 Fundamental studies2004-------------2007 Preclinical2007-------------2010 Clinical
Diverse Funding Source and Timeline
National Science FoundationCommonwealth of VirginiaAmerican Red CrossNIH/NIADProgenetics LLCMinistry of BrazilIndian Department of Biotechnology
Executive Council Meeting
P-1 / V-101
Pig Milk (FIX)
P-2 / V-102
Dilution Buffer 1
P-3 / MX-101
P-4 / BC-101
Cent / Fats remove
P-5 / V-103
Blending / 1:1
waste cent
P-8 / C-102
Heparin Capture
waste hep
P-10 / C-103
IONX
P-13 / DF-101
Conc / DiafP-14 / FDR-101
Freeze Drying / Vialswaste diaf 2
Lyophylized FIX
vent Lyoph
P-9 / V-104
P-15 / V-105
Dilution Buffer 2
P-7 / V-106
Viral Inactivation Buffer
P-16 / V-107
Viral Inact
P-17 / DE-101
Filtration
S-123
waste solid
Buffer 1
In Milk
anti-Vir
S-112
S-104
Buffer 2
P-19 / MX-102
S-105
S-102
S-114
P-20 / DF-102
Diafiltration
S-115
exch feed diaf1
exch feed diaf2
vent milk
vent antVi
vent buf 1
P-21 / MX-103
S-108
S-111S-137
vent
wa ionx
eq ionx
rg ionx
el ionx
wa hep
eq hep
rg hep
el hep
Fatsvent blend1
vent antVir
vent blend 2
S-109
w ionx
P-11 / DF-103
Diafiltration
exch feed diaf3
waste diaf 3
S-117
w diaf 1
P-6 / DPP-101
Fluid Flow
S-103S-106
P-18 / DPP-102
Fluid Flow
S-101
S-107
P-12 / C-101
FPLC IONX
wa ix2eq ix2
S-118
rg ix2
el-A ix2
el-B ix2
S-125
w ionx2
S-110
S-113
Transgenic Pig
High Purity Biologic formulation:Intravenous, Oral, Buccal, IN, IT Delivery
Recombinant Protein in Milk
Large Scale Purification Process
Pig Chromosome with Milk Production Gene through cloning or microinjection
Complex Recombinant Protein Production Process
Executive Council MeetingSaraf, UNL 2006rsaraf@unlnotes.unl.edu
Resolution is 20 mm which is 2-fold smaller than human finger;and 100 fold smaller than the currentElectronic MEMS devices.
Nano Touch Sensors Ravi Saraf, Chemical Engineering, rsaraf@unl.edu
Disphering cancer by texture: The surgeon has a resolution of our sensor is ~20 mm.
Giving surgeon the sensation of human touch during minimally invasive procedure:
Executive Council Meeting
(c)
(d)
-20 -10 0 10 20-20
-15
-10
-5
0
5
10
15
20-8 -6 -4 -2 0 2 4 6 8
-4
-2
0
2
4
Curr
ent, n
A 150 K 50K
Curr
ent, n
A
Bias, V
Bias, V
296 K (room temp.)
(e)0 100 200 300 400 500
0
10
20
30
40
50
60
70
A: add methanolB: remove methanolC: add methanolD: remove methanolE: air tightF: add isopropenolG: remove isopropenolH: add ethenol
pichia pastoris (yeast) on30 m long Au Nanoprticle Necklace: Metabolism Signal
H
C E
D
F G
A
B
Curr
ent at
4 V
bia
s, n
A
Time, mins.
(f)
Single Electron Nanodevice to Measure Electrochemical Activity in a Single CellRavi Saraf, Chemical Engineering, rsaraf@unl.edu
• Combinatorial microarray with low false positive: Using the necklace as nano-electrode and combining with our combinatorial electrochemistry device, will allow us to measure perform microarray analysis using cells as the transduction agency.
• Live Bio-transistor: Use cell as a gating device to make electronics that is “intelligent” and “adaptable”.
• Power generation: Use the charge production form the cell (such as ATP cycle) to convert food directly to electricity.