2009 NMVB Conference2009 NMVB Conference

NYU Dept of Cardiothoracic SurgeryNYU Dept of Pathology

Friday December 4th, 2009

Personnel @ NYUPersonnel @ NYU• Dept of Cardiothoracic Surgery:

• Harvey Pass, MD (surgeon, NYU steering committee member & PI)

• Audrey Sorensen, RN (research nurse coordinator)

• Samantha Lincoln (data manager)

• Ryan Harrington (lab technician, thoracic lab)

• Angela Pittman (research project coordinator)

• Dept of Pathology:• Jonathan Melamed, MD (pathologist, NYU steering committee member)

• Jacinta Small (lab technician, pathology lab)

• Tian He (research coordinator; previously Timothy Jolis)

• Xiangtian Kong (pathologist, pathology review)

2008-20092008-2009• Logistic improvements:

• Streamlined specimen/case-notification process

• Refined tissue procurement process with

response team and procurement kits

• Synchronized local data

with MVB database

• Progression on 5-year plans:

• Frozen TMA

• NYU MVB Paraffin TMA 1

Prospective cases: 63Retrospective cases: 157 Total # of cases: 220

YearRetrospective

CasesProspective

CasesOverall Total

2007

157 31 188

2008

0 21 21

2009

0 11 11

Total: 220

Mesothelioma AccrualMesothelioma Accrual

Tissue Collected Tissue Collected (research blocks)(research blocks)

Collection Method # of Blocks / Samples of Frozen Tissue

OCT 124

FOIL 130

Paraffin Blocks 288

Year New cases collected

Collection Method

Frozen tissue only

Paraffin + Frozen tissue

Paraffin tissueonly

2008 7 0 6 1

2009 9 2 7 0

• Emphasis on accruing extrapleural pneumonectomy cases

Meso accrual data (cont’d)Meso accrual data (cont’d)

Progression on 5 yr plans-Frozen TMAProgression on 5 yr plans-Frozen TMA

Frozen TMA

Frozen Array mold kit

Frozen TMAFrozen TMA

Normal lung tissue

Tumor tissue (mesothelioma)

MVB #s indicatedistinct patients

N=13 ptsmvb22

21mvb22

21          

mvb2189

mvb2215

mvb2219  

mvb2189

mvb2215

mvb2219

mvb2190

mvb2191

mvb2197  

mvb2190

mvb2191

mvb2197

mvb2217

mvb2214

mvb2199  

mvb2217

mvb2214

mvb2199

mvb2203

mvb2211

mvb2213  

mvb2203

mvb2211

mvb2213

mvb2212      

mvb2212    

Frozen TMA: Equipment & AccessoriesFrozen TMA: Equipment & Accessories

• NYU MVB Paraffin TMA 1NYU MVB Paraffin TMA 1• Design Phase:

• N= cases/ Block X 4 B• 4 TMA Blocks (same cases on each

block)• Control tissues, cell lines, frozen-

to-formalin tissue (adv: standardizedprocessing)

• Construction Phase: MTA II• Microtomy: Limited number of sections;

histocollimator• Storage: In desiccators under nitrogen

atmosphere• High resolution scan of slide for website

Plans for Paraffin TMAPlans for Paraffin TMA

Sample paraffin TMA

Paraffin TMA: Equipment & AccessoriesParaffin TMA: Equipment & Accessories

Desiccators

Tissue Micro-Arrayer

Proposed map for Paraffin TMAProposed map for Paraffin TMA

  Cell Lines

  Tumor

  Control

  Liver

Column →

Row ↓ 0 1 2 3 4 5 6 7 8 9 10 11 12 13A LiverB                        C                        D                        E                        F                        G                  H                        I                        J                        K                        L                        MN                O                P                Q                R                

Special Thanks to…Special Thanks to…

Today’s conference attendeesToday’s conference attendees

And the entire NMVB teamAnd the entire NMVB team