2.2 Prokaryotic Cells. “primitive cells” cells that lack membrane-bound organelles ie: no...

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2.2 Prokaryotic Cells

“primitive cells”

cells that lack membrane-bound organelles

ie: no nucleus, no mitochondria, no vacuoles, etc

Ex: bacteria (ie E. Coli) – found in soil, water, on your skin, in your intestine

Ex: blue green algae

Prokaryotic Cells

organisms that are made of prokaryotic cells.

Most prokaryotes are unicellular, such as bacteria, however, they can be multicellular too, such as blue green algae

Prokaryotes

Prokaryote Cell Structue

CELL SURFACE MEMBRANE / PLASMA MEMBRANE: Thin layer composed of phospholipids Controls the entry/ exit of substance via active

or passive transport Selectively permeable

Prokaryote Cell Parts

CELL WALL: Rigid to semi-rigid wall surrounding the plasma

membrane. Contains pores to allow passage of materials in

and out of the cell. The Cell wall supports the cell and provides

shape

Prokaryote Cell Parts

SLIME CAPSULE: Protective outside layer, found outside the cell

wall Also known as “surface layer” or the “S-layer” Protects the cell from environmental dangers Contains proteins and or glycoproteins

Prokaryote Cell Parts

Cytoplasm: The “inside” of the cell Contains cytosol (fluid of the cell) Contains the enzymes (biological catalysts) for

catalyzing chemical reactions of metabolism Contains ribosomes and naked DNA No membrane bound organelles!

Prokaryote Cell Parts

NUCLEOID: Region of the prokaryotic cytoplasm where

genetic information is found (1 main chromosome and plasmids)

The 1 chromosome is made of NAKED DNA This means that the DNA is not bound to proteins

(like histones) This reduces the amount of space they take up Usually circular

Prokaryote Cell Parts

PLASMID: Small, circular, naked, DNA Smaller, and contains less information than the

chromosome. Found in the nucleoid region

Prokaryote Cell Parts

Mesosome: Indentation in the cell membrane of bacteria Unknown function, produced by chemical

fixation techniques.

Prokaryote Cell Parts

RIBSOSOME: Made of RNA and proteins Makes proteins Less dense than eukaryotic ribosomes

Prokaryote Cell Parts

FLAGELLUM: threadlike, motile proteins structures and are

used for locomotion Cell may have none, 1, or several

Prokaryote Cell Parts

PILI (plr) / PILUS (singlular): Thin protein tubes found outside the plasma

membrane and extend out from the slime capsule

Attachment Pili Have sticky ends that allow it to attach to

surfaces (i.e. another cell) Conjugation Pili (Sex Pili)

Longer Build a bridge between 2 bacterial cells allowing

for exchange of DNA (conjugation)

Prokaryote Cell Parts

Prokaryotic cells divide asexually by binary fission (similar to mitosis)

They duplicate their chromosome (their circular DNA) and then split in half (each new daughter cell gets half the cytoplasm and 1 chromosome)

Prokaryotic Reproduction

Since binary fission is a form of asexual reproduction, the daughter cells produced are identical to the original parent cell.

Conjugation (with the help of conjugation pili) allows for genetic variation – allows for a new combination of DNA

Annotate and Identify Structures from and electron micrograph of E. Coli

ribosome

Nucleoid region (DNA, plasmid)Cell wall

cytoplasmPlasma membrane

No visible slime capsule in this diagram

PILUS

SLIME LAYER

Metabolic Activity of Prokaryotic Cells

1. Photosynthesis: light + CO2 + H2O C6H12O6 + O2

2. Nitrogen Fixation: Convert nitrogen from an unusable from to a usable form

3. Fermentation: convert organic substances into simpler substances (ie sugar into ethanol)