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AP BiologyChapter 3: Structure and Function of Macromolecules
(Independently brush up on Ch 2 and Ch 3.1)
Enduring UnderstandingsEnduring Understandings IV. A. Interactions within biological systems lead to complex
properties. 1. The subcomponents of biological molecules and their sequence determine
the properties of that molecule. B. Competition and cooperation are important aspects of
biological systems. 1. Interactions between molecules affect their structure and function.
C. Naturally occurring diversity among and between components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
IV. A. Interactions within biological systems lead to complex properties.
1. The subcomponents of biological molecules and their sequence determine the properties of that molecule.
B. Competition and cooperation are important aspects of biological systems.
1. Interactions between molecules affect their structure and function. C. Naturally occurring diversity among and between
components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
Macromolecules
Smaller organic molecules join together to form larger molecules macromolecules
4 major classes of macromolecules: carbohydrates lipids proteins nucleic acids
H2O
HO
HO H
H HHO
Polymers
Long molecules built by linking repeating building blocks in a chain monomers
building blocks repeated small units
covalent bonds
H2O
HO
HO H
H HHO
How to build a polymer
Synthesis joins monomers by “taking” H2O out
one monomer donates OH–
other monomer donates H+ together these form H2O
requires energy & enzymes
enzyme
Dehydration synthesis - Condensation reaction, in which the lost molecule = H2O
Dehydration synthesis - Condensation reaction, in which the lost molecule = H2O
Condensation reaction Condensation reaction
Dehydration Synthesis
H2O
HO H
HO H HO H
How to break down a polymer Digestion
use H2O to breakdown polymers reverse of dehydration synthesis cleave off one monomer at a time
H2O is split into H+ and OH–
H+ & OH– attach to ends requires hydrolytic enzymes releases energy
HydrolysisHydrolysis
enzyme
Discussion
Under what circumstances would you expect to find a cell conducting a great deal of dehydration synthesis?
In which organs or under what circumstances would you expect to find body cells conducting hydrolysis?
Variety of Polymers Every cell has thousands of
varieties of macromolecules These molecules are constructed
from only 40 to 50 common monomers
Analogy: 26 letters of the alphabet can be combined to form millions of words Shortcoming: macromolecules are
much longer than the average word and they can be branched or 3D.
Carbohydrates
Carbohydrates
a.k.a. wheeee energy! :D
Carbohydrates Carbohydrates are composed of C, H,
Ocarbo - hydr - ate
CH2O)x C6H12O6
Function: energy energy storage raw materials structural
materials
Monomer: sugars (monosaccharide)
sugar sugar sugar sugar sugar sugar sugarsugar
C6H12O6(CH2O)x
Sugars
Most names for sugars end in -ose Classified by number of carbons
6C = hexose (glucose) 5C = pentose (ribose) 3C = triose (glyceraldehyde)
6 5 3
Sugar structure
5C & 6C sugars form rings in solution
Numbered carbons
C
CC
C
CC
1'
2'3'
4'
5'
6'
O
energy stored in bondsenergy stored in bonds
You’ll see this come back in our DNA unit…
Simple & complex sugars Monosaccharides
simple 1 monomer sugars Ex: glucose, galactose
Disaccharides 2 monomers
Ex: sucrose, lactose
Polysaccharides 3+ monomers
Ex: starch, cellulose, glycogen
Building sugars Dehydration synthesis to form
glycosidic bond
|fructose
|glucose
monosaccharides
|sucrose
(table sugar)
disaccharide
H2O
Polysaccharides
Polymers of sugars costs little energy to build easily reversible = release energy
Functions: energy storage
starch (plants) glycogen (animals)
in liver & muscles structure
cellulose (plants) chitin (arthropods & fungi)
Polysaccharide diversity Molecular structure determines function - a major
theme!
Isomers of glucose Different structure = connect to the next monomer in
the chain differently = different 3D structure. Starch - helical. Cellulose - straight, with free OH to
bond to neighboring celluloses = rigid structure!
in starch in cellulose
Cellulose Most abundant organic
compound on Earth herbivores have evolved a
mechanism to digest cellulose most carnivores have not
that’s why they eat meat to get their energy & nutrients
cellulose = undigestible roughage
Helpful bacteria How can herbivores digest cellulose so
well? BACTERIA live in their digestive systems &
help digest cellulose-rich (grass) meals
Ruminants
Discussion In EXACTLY 20 words, summarize the
most important point or points to remember about carbohydrates.
Lipids: Fats & Oils
Enduring UnderstandingsEnduring Understandings IV. A. Interactions within biological systems lead to complex
properties. 1. The subcomponents of biological molecules and their sequence determine
the properties of that molecule. B. Competition and cooperation are important aspects of
biological systems. 1. Interactions between molecules affect their structure and function.
C. Naturally occurring diversity among and between components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
IV. A. Interactions within biological systems lead to complex properties.
1. The subcomponents of biological molecules and their sequence determine the properties of that molecule.
B. Competition and cooperation are important aspects of biological systems.
1. Interactions between molecules affect their structure and function. C. Naturally occurring diversity among and between
components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
Lipid Primary Function:
long term energy storageconcentrated energy
Lipids
Lipids are composed of C, H, O long hydrocarbon chains (H-C)
“Family groups” fats phospholipids steroids
Do not form polymers big molecules made of smaller
subunits not a continuing chain
Same as carbohydrates, but different structure = different function!
Fats
Structure: glycerol (3C alcohol) + fatty acid
fatty acid = long HC “tail” with carboxyl (COOH) group “head”
dehydration synthesis
H2O
enzyme
Building Fats
Triglycerol 3 fatty acids linked to glycerol ester linkage between OH & COOH
hydroxyl carboxyl
Dehydration synthesis
dehydration synthesis
H2O
H2O
H2O
H2O
enzyme
enzyme
enzyme
Discussion
What kind of molecule would you expect to be hydrophobic - polar or non-polar?
Why?
Do you think lipids (such as fats, oils, waxes) are probably polar or non-polar?
Why?
Fats store energy Long HC chain
polar or non-polar? hydrophilic or hydrophobic?
Functions: energy storage
2x carbohydrates cushion organs membranes & waterproofing insulates body
think whale blubber!
Discussion Show me a human who doesn’t want to
eat this and I’ll show you a LIAR. LIESSSSSSS
Knowing their major functions, hypothesize: what occurred in evolutionary history that led to humans enjoying the tastes of fatty and sugary foods?
Structure & Function Saturated Fats
All C bonded to H No C=C double bonds
long, straight chain most animal fats solid at room temp.
contributes to cardiovascular disease (atherosclerosis) = plaque deposits
Structure & Function Unsaturated Fats
C=C double bonds in the fatty acids plant & fish fats vegetable oils liquid at room temperature
the kinks made by doublebonded C prevent the molecules from packing tightly together
Discussion Unsaturated fats are widely
considered to be healthier (sometimes called “good fats” for short) than saturated fats.
Why might this be? (Hint: think of their structures, and what they might mean for how easily the body would use them for energy vs. storing them in fat cells)
Phospholipids Structure:
glycerol + 2 fatty acids + PO4
PO4 = negatively charged
Phospholipids Hydrophobic or hydrophilic?
fatty acid tails = PO4 head = split “personality”
interaction with H2O is complex & very important!
“repelled by water”
“attracted to water”
hydrophobic
hydrophillic
Phospholipids in water Hydrophilic heads “attracted” to H2O
Hydrophobic tails “hide” from H2O can self-assemble into “bubbles”
called micelles can also form a phospholipid bilayer Early Earth history - a cell part that self-
assembles!
bilayer
water
water
Why is this important?
Phospholipids create a barrier in water define outside vs. inside they make cell membranes!
Steroids
Structure: 4 fused C rings + ??
different steroids created by attaching different functional groups to rings
different structure creates different function
examples: cholesterol, sex hormones
cholesterol
Cholesterol Important cell component
animal cell membranes precursor of all other steroids
including vertebrate sex hormones high levels in blood contribute to
cardiovascular disease
Cholesterol
helps keep cell membranes fluid & flexible
Important component of cell membrane
From Cholesterol Sex Hormones What a big difference a few atoms can make!
Discussion In EXACTLY 20 words, summarize the
most important point or points to remember about lipids.
Nucleic AcidsInformation
storage
Enduring UnderstandingsEnduring Understandings IV. A. Interactions within biological systems lead to complex
properties. 1. The subcomponents of biological molecules and their sequence determine
the properties of that molecule. B. Competition and cooperation are important aspects of
biological systems. 1. Interactions between molecules affect their structure and function.
C. Naturally occurring diversity among and between components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
IV. A. Interactions within biological systems lead to complex properties.
1. The subcomponents of biological molecules and their sequence determine the properties of that molecule.
B. Competition and cooperation are important aspects of biological systems.
1. Interactions between molecules affect their structure and function. C. Naturally occurring diversity among and between
components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
proteinsproteins
DNADNA
Nucleic Acids
Function:genetic material
stores informationgenesblueprint for building
proteins DNA RNA proteins
transfers informationblueprint for new cellsblueprint for next
generation
Nucleic Acids
Examples: RNA (ribonucleic acid)
single helix DNA (deoxyribonucleic acid)
double helix
Structure: monomers = nucleotides
RNA
DNA
Nucleotides
3 parts nitrogen base (C-N ring) pentose sugar (5C)
ribose in RNA deoxyribose in DNA
phosphate (PO4) group
Nitrogen baseI’m the
A,T,C,G or Upart!
Types of nucleotides
2 types of nucleotides different nitrogen bases purines
double ring N base adenine (A) guanine (G)
pyrimidines single ring N base cytosine (C) thymine (T) uracil (U)
Purine = AG“Pure silver!”
Nucleic polymer Backbone
sugar to PO4 bond phosphodiester bond
new base added to sugar of previous base
dehydration synthesis again! polymer grows in one
direction N bases hang off the
sugar-phosphate backboneDangling bases?
Why is this important?
Pairing of nucleotides
Nucleotides bond between DNA strands H bonds purine :: pyrimidine A :: T
2 H bonds G :: C
3 H bonds
Matching bases?Why is this important?
DNA molecule Double helix
H bonds between bases join the 2 strands A :: T C :: G
Like carbohydrates, strands have direction that matters to structure The end with a dangling phosphate (5’)
can’t have any more bases added to it, unlike the other end (3’)
H bonds?Why is this important?
Copying DNA Replication
2 strands of DNA helix are complementary have one, can build other have one, can rebuild the
whole
Matching halves?Why is this
a good system?
Interesting note…
Ratio of A-T::G-C affects stability of DNA molecule 2 H bonds vs. 3 H bonds biotech procedures
more G-C = need higher T° to separate strands
high T° organisms many G-C
parasites many A-T (don’t know why)
Another interesting note… ATP
Adenosine triphosphate
++
modified nucleotide adenine (AMP) + Pi + Pi
Discussion In EXACTLY 20 words, summarize the
most important point or points to remember about nucleic acids.
ProteinsMultipurpose
molecules
Enduring UnderstandingsEnduring Understandings IV. A. Interactions within biological systems lead to complex
properties. 1. The subcomponents of biological molecules and their sequence determine
the properties of that molecule. B. Competition and cooperation are important aspects of
biological systems. 1. Interactions between molecules affect their structure and function.
C. Naturally occurring diversity among and between components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
IV. A. Interactions within biological systems lead to complex properties.
1. The subcomponents of biological molecules and their sequence determine the properties of that molecule.
B. Competition and cooperation are important aspects of biological systems.
1. Interactions between molecules affect their structure and function. C. Naturally occurring diversity among and between
components within biological systems affects interactions with the environment.
1. Variation in molecular units provides cells with a wider range of functions.
Proteins
Most structurally & functionally diverse group
Function: involved in almost everything enzymes (pepsin, DNA polymerase) structure (keratin, collagen) carriers & transport (hemoglobin, aquaporin) cell communication
signals (insulin & other hormones) receptors
defense (antibodies) movement (actin & myosin) storage (bean seed proteins)
Proteins
Structure monomer = amino acids
20 different amino acids polymer = polypeptide
protein can be one or more polypeptide chains folded & bonded together
large & complex molecules complex 3-D shape
Rubisco
hemoglobin
growthhormones
H2O
Amino acids Structure
central carbon amino group carboxyl group (acid) R group (side chain)
variable group different for each amino acid confers unique chemical properties to
each amino acid like 20 different letters of an alphabet can make many words (proteins)
—N—H
HC—OH
||O
R
|—C—
|
H
Building proteins
Peptide bonds covalent bond between NH2
(amine) of one amino acid & COOH (carboxyl) of another
C–N bond
peptidebond
dehydration synthesisH2O
Building proteins Like carbs & nucleic acids,
polypeptides have direction that matters! N-terminus = NH2 end C-terminus = COOH end
repeated sequence (N-C-C) is the polypeptide backbone
can only grow in one direction, N -> C
Protein structure & function
hemoglobin
Function depends on structure 3-D structure
twisted, folded, coiled into unique shape
collagen
pepsin
Primary (1°) structure Order of amino acids in chain
amino acid sequence determined by gene (DNA)
slight change in amino acid sequence can affect protein’s structure & its function even just one amino acid
change can make all the difference! Remember sickle-cell anemia?
lysozyme: enzyme in tears & mucus that kills bacteria
Sickle cell anemia
I’mhydrophilic! I’m hydrophobic!
Just 1out of 146
amino acids!
Non- polar valine “tries tohide” from water of cellby sticking to anotherhemoglobin molecule
Secondary (2°) structure
“Local folding” folding along short sections of
polypeptide interactions between
adjacent amino acids H bonds
weak bonds between R groups
forms sections of 3-D structure -helix -pleated sheet
Secondary (2°) structure
Tertiary (3°) structure
“Whole molecule folding” interactions between distant amino
acids hydrophobic interactions
cytoplasm is water-based
nonpolar amino acids cluster away from water
H bonds & ionic bonds disulfide bridges
covalent bonds between sulfurs in sulfhydryls (S–H)
anchors 3-D shape
Quaternary (4°) structure
More than one polypeptide chain bonded together only then does polypeptide become
functional protein
Collagen = skin & tendon structure Hemoglobin = holds O2
Sequence -> Structure Structure ->
Function
amino acid sequence
peptide bonds
1°
determinedby DNA R groups
H bonds
R groupshydrophobic interactions
disulfide bridges(H & ionic bonds)
3°multiple
polypeptideshydrophobic interactions
4°
2°
Protein denaturation
Unfolding a protein conditions that disrupt H bonds,
ionic bonds, disulfide bridges temperature pH salinity
alter 2° & 3° structure alter 3-D shape
destroys functionality some proteins can return to their
functional shape after denaturation, many cannot
Discussion In EXACTLY 20 words, summarize the
most important point or points to remember about proteins.
Macromolecule Review
Carbohydrates
Structure / monomer monosaccharide
Function energy raw materials energy storage structural compounds
Examples glucose, starch, cellulose,
glycogen
glycosidic bond
Lipids
Structure / building block glycerol, fatty acid, cholesterol, H-C
chains Function
energy storage membranes hormones
Examples fat, phospholipids, steroids
Nucleic acids
Structure / monomer nucleotide
Function information storage
& transfer Examples
DNA, RNA
phosphodiester bond
Proteins
Structure / monomer amino acids levels of structure
Function enzymes defense transport structure signals receptors
Examples digestive enzymes, membrane
channels, insulin hormone, actin
peptide bond
Discussion In EXACTLY 20 words, summarize the
major theme/s in studying biomacromolecules. (And no, wise guy, I don’t mean “they’re hard.”)