Post on 31-Aug-2020
transcript
Benzofuran pyran compound rescues rat and human osteoblast from lipotoxic effect of
palmitate by inhibiting lipid biosynthesis and promoting stabilization of RUNX2
Authors: Ashish Kumar Tripathi1, Divya Rai1, Priyanka Kothari1 Pragati Kushwaha2, Shradha
Sinha1, Anirban Sardar1 Koneni V. Sashidhara2, Ritu Trivedi1*
*Corresponding Author: Ritu Trivedi
Endocrinology Division
CSIR-Central Drug Research Institute
B.S. 10/1, Sector 10, Jankipuram Extension
Sitapur Road, Lucknow, Uttar Pradesh 226031, INDIA
Phone: 91-522-2771940-42 (Ext. 4397)
Fax: 91-522-2771941
Email- ritu_trivedi@cdri.res.in
ritu_pgi@yahoo.com
S 1 Result
Screening of Benzofuran Pyran Compounds on palmitate induced lipotoxic in vitro
model
Compound 4j, neither increases the cell viability nor osteoblast differentiation (Fig.S1A and
B). Compound 4k enhanced cell viability at 500pm and 1.0µM (Fig.S1C) but failed to
enhance osteoblast differentiation in presence of palmitate (Fig.S1D).
(B)
1
ALP activity (OD 405)
Palmitate 250μM
##
(A)
(D)
Figure legend
Fig S1 Compound 4j did not rescue osteoblasts from lipotoxic of palmitate (A) and nor
increase ALP activity in the presence of palmitate (B). Compound 4k significantly increased
cell viability at 500pm and 1µM concentration (C) but failed in increasing ALP activity (D).
Data were presented as the mean ± SE, # p˂0.05, ## p˂ 0.005, ### p˂0.0005 versus control
group and *p˂0.05, **p˂0.005, ***p˂0.0005 versus palmitate group.
2
#
ALP activity (OD 405)
Palmitate 250μM
4k(C)