Benefits of scientific cooperation among European countries in medical research

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Daniela Jezova

Institute of Experimental EndocrinologyInstitute of Experimental EndocrinologySlovak Academy of SciencesSlovak Academy of Sciences

BratislavaBratislava

Title of the Project

„Reduction of Negative Impact of Environmental Factors on Human Health“

Co-ordinator: Assoc.Prof. Daniela Ježová

Project of the Centre of Excellence supported by European Union

Institute of Experimental EndocrinologySlovak Academy of SciencesBratislava, Slovakia

Coordinator: Assoc. Prof. Daniela Ježová

Main „mission” of this Centre

To provide experimental and clinical data leading to specific recommendations aimed at reducing the negative impact of environmental factors on human health

STRESSNUTS

obtaining high quality scientific data with relevance to clinical research

significant improvement of the training process of young researches both from EU and Slovakia

active and broad cooperation among European scientists

Main positive achievements

Most important scientific result (one selected from many)

Evidence has been obtained to support

further clinical research on the following

substances to modulate hormone release

and treat the negative impact of demanding

stressful situations, anxiety and depression:

mood stabilizers

extract of Ginkgo biloba

pentoxifylline

antidepressants

selected amino acids

Salivary cortisol (ng/ml)

0 15 30 45min

stress

time: p<0.001treatment: p<0.05

placebo

lamotrigine

STRESSNUTS

Public speech

Prolonged anticipationProlonged speechEmotional topicsVideo recording of speechStanding during speechUse of microphoneBright lightOpen space

0 15 30 45 60 90

placebo

amino acids

time: p=0.023treatment: p=0.004

stress

ACTH(pg/ml)

0 15 30 45 60 90

placebo

amino acids

time: p<0.001treatment: p=0.009

stress

Salivary cortisol(ng/ml)

0 15 30 45 60 90

placebo

amino acids

time: 0.004treatment: p<0.001

stress

Plasma cortisol(μg/100ml)

Amino acid supplementation

Serotonine and stress

0 30 45 60

p<0.05

ACTH(pg/ml)

Enhancement of stress-induced ACTH release by treatment with citalopram and tianeptine

insulin

020406080

basal values

„Postdoctoral fellow“Silvia Anette Wein, PhD., GermanyLaboratory Chief : Ing. E. Šeböková, DSc.

„Postdoctoral fellow“Ibolya Bodnár, PhD., HungaryLaboratory Chief : RNDr. R. Kvetňanský, DSc.

Long-term stays in the Centre

„Postdoctoral fellow“Sven Mostböck, PhD., Austria Laboratory Chief: Ing. J. Brtko, PhD.

„Senior Visitor“

Prof. Barbro B. Johansson, M.D., Sweden

„PhD. Student“Aikaterini Makatsori, M.D., GreeceTutor : Assoc. Prof. D. Ježová

1. Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D.:Modulation of neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release inhibiting drug lamotrigine. Neuroendocrinology 79:34-42, 2004

2. Makatsori A., Duncko R., Schwendt M., Moncek F., Johansson B. B., Jezova D.: Voluntary wheel running modulates glutamate receptor subunit gene expression and stress hormone release in Lewis rats. Psychoneuroendocrinology 28: 702-714, 2003

3. Makatsori A., Jezova D.: Drug-induced fetal damage and the consequences for neuroendocrine function. Pediatric Endocrinology vol. 2: 53-61, 2003

4. Jezova D., Skultetyova I., Makatsori A., Moncek F., Duncko R.: Hypothalamo-pituitary-adrenocortical axis function and hedonic behavior in adult male and female rats prenatally stressed by maternal food restriction. Stress 5: 177-183, 2002

5. Schwendt M., Dunčko D., Makatsori A., Johansson B.B., Ježová D.:Involvment of glutamate neurotransmission in the development of excessive wheel running in Lewis rats. Neurochemical Research ,28: 653-657,2003

6. Bakos J., Duncko R., Makatsori A., Pirnik Z., Kiss A., Jezova D.: Prenatal immune challenge affects growth, behavior, and brain dopamine in the offspring. Annals of the New York Academy of Sciences (in press, 2004)

7. Pistovcakova J., Makatsori A., Sulcova A., Jezova D.: Felbamate reduces hormone release and locomotor hypoactivity induced by repeated stress of social defeat in mice. European Neuropsychopharmacology (in submission)

8. Makatsori A., Dubovicky M., Ujhazy E., Duncko R., Bakos J., Jezova D.: Neuroendocrine changes in adult female rats prenatally exposed to phenytoin. Toxicology and Applied Pharmacology (in submission)

9. Jezova D., Makatsori A., Duncko R., Moncek F., Jakubek M.: High trait anxiety in healthy subjects: association with low neuroendocrine responses during psychosocial stress. Progress in Neuro-psychopharmacology and Biological Psychiatry (in submission)

List of publications of the PhD student A. Makatsori, M.D.

X. International Congress of the Czech and Slovak Neurochemical Society

„NEUROSCIENCE IN THE THIRD MILLENNIUM“

June 1 – 5, 2002, ČASTÁ, SLOVAKIA

Institute of Experimental EndocrinologyCentre of Excellence supported by European Commission

Bratislava, Slovakia

www.neuromeeting2002.savba.sk

Organised by

INSTITUTE OF EXPERIMENTAL ENDOCRINOLOGYCentre of Excellence of European Commission, Bratislava, Slovakia

Slovak Academy of Sciences

June 28 - July 3, 2003 Smolenice Castle, Slovakia

Work package number and title: WP 14

Twinning and Networking with successful Centres of Excellence Objectives originally planned - To increase the cooperation among scientists in the Central European region.- To increase the scientific quality of meetings and training programs organized by successful Centres and other European laboratories

Change in the quality of the Centre

The Centre • has changed into an institution that has more foreign

scientists involved in local research than the number of local researchers working abroad

• has become an international centre for the education of young scientists

STRESSNUTS

Work package number and title: WP 12

Twinning and Networking with Institute of Adaptive and Space Physiology, University of Graz,Graz, Austria Objectives originally planned - To increase the quality of the research of the Centre in the field of renin-angiotensin systems and body fluids regulation- To apply the experience of the twinning laboratory in the research programme of the Centre

Twinning Laboratory

Institute of Adaptive and Space Physiology and Department of Physiology, University of Graz, Austria

Publications and papers

Hinghofer-Szalkay H., Loder I., Pilz K., Rossler A., Jezova D. : Gravity and cardiovascular regulation: Combined stimulation paradigm. Journal of Gravitational Physiology 10: 89-90, 2003

Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D. : Modulation of

neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release-inhibiting drug lamotrigine. Neuroendocrinology 79: 34-42, 2004

Hinghofer-Szalkay H, Haditsch B, Loder I, Pilz K, Rössler A, Jezova D. Head down tilt at –6° to –24° can neutralize the cardiovascular effects of LBNP at –15 or –35 mmHg. Aviat Space Environ Med 2004; 75: 947-51

Hinghofer-Szalkay HG, László Z, Passath H, Pilz K, Rössler A, Jezova D., Scharfetter H.: Sodium intake does not influence bioimpedance.derived extracellular volume loss in head-down rest. Aviat. Space Environ. Med. 75: 1036-41, 2004

Loder I., Roessler A., Wurzinger G., Duncko R., Jezova D., Hinghofer-Szalkay H.: Adrenomedullin and elements of orthostatic competence after 41 hours water submersion in men. Life Sci. 2004

Stress model

relaxing phase

anticipation 15 min

speech 15 min

relaxing phase

Stress model

Postural changes during public speech tests contribute to the stress response Martin Mlynarik a, Aikaterini Makatsori a, Iveta Matyiova a, Helmut G. Hinghofer-Szalkay b,Daniela Jezova a,*

a Laboratory of Pharmacological Neuroendocrinology, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, Bratislava 83306, Slovakiab Institute for Adaptive and Spaceflight Physiology, Wormgasse 9, A-8010 Graz, Austria Running head: Postural changes and stress response

Benefits of scientific cooperation among European countries in medical research

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