Bettina Agnes Lee Josh Christian Protacio

Justin Stuart Tcheng Darla Teylan

OSMOSIS IN HUMAN RED BLOOD

CELLS

INTRODUCTION

Human red blood cells function for

transport, and for circulation of oxygen,

nutrients, wastes and other materials

throughout the body. They are enucleated

in order to create more space for

hemoglobin and are mainly found

within the heart and blood vessels

and circulatory system.

WHAT IS OSMOSIS?

Movement of molecules from an

area of higher concentration to an

area of lower concentration

through a SEMI-PERMEABLE

MEMBRANE

IN A HYPOTONIC

SOLUTION

Concentration of solutes inside

the cell is greater than the

concentration of solutes of its

environment

Result: Cell will increase in size

because solvent will enter the

cell.

IN AN ISOTONIC

SOLUTION

The concentration of solutes in

and out of the cell is equal. Water

flows across the membrane at the

same rate in both directions.

Result: Cell will remain the same

size

IN A HYPERTONIC

SOLUTION

Concentration of solutes inside

the cell is less than the

concentration of solutes of its

environment

Result: Cell will shrink because

solvent will go out of the cell.

HEMOLYSIS

The rupture or destruction of

red blood cells.

*similar to plasmolysis in

plant cells

CRENATION

The shrinkage of red blood cells

resulting in crenate margins

Crenate

- Having the margin or surface cut

into rounded scallops

OBJECTIVES

At the end of this activity we should be able

to

1. Observe for changes in the shape of

cells

2. Predict the direction of the diffusion of

solutes

3. Understand the reasons for such

phenomenons

HYPOTHESES

Null Hypothesis:

No change in the size of the human red

blood cells in different NaCl concentration

solutions

Alternative Hypothesis:

Human red blood cells will change in size in

different NaCl concentration solutions.

METHODOLOGY

• Microscope

• 3 Glass Slides

• 3 Cover slips

• Ocular micrometer

• Stage micrometer

• Sterile lancet

• 0.07M NaCl solution

• 0.15M NaCl solution

• 0.30M NaCl solution

• Alcohol

• Cotton ball

• Dropper

• Willing volunteer

• Timer

MATERIALS AND

EQUIPMENT

1.

Calibrate the

microscope

PROCEDURE

2.

Label 3 slides

and collect 3

drops of

blood from a

willing

volunteer

PROCEDURE

3. Add a drop of

0.07M NaCl solution

to first slide, 0.15M

NaCl solution to the

second slide, and

0.30M NaCl solution

to the third slide. Miz

the blood and NaCl

solution thoroughly

and smear the

mixture evenly.

PROCEDURE

4. With the

microscope at

400x

magnification,

measure the size

of the red blood

cells every 5

minutes for one

hour. (Use an

ocular micrometer

and compute for

the size using the

calibration factor)

PROCEDURE

RESULTS

0.07M

INITIAL VS 60 MINUTES

0.07M

INITIAL VS 60 MINUTES

0.15M

INITIAL VS 60 MINUTES

0.15M

INITIAL VS 60 MINUTES

0.30M

INITIAL VS 60 MINUTES

0.30M

INITIAL VS 60 MINUTES

SIZE OF RBC PER CONCENTRATION OF NACL

SOLUTION

Time 0.07 M 0.15 M 0.30 M

Initial 0.015mm 0.015mm 0.015mm

5 minutes 0.015mm 0.015mm 0.01125mm

10 minutes 0.015mm 0.015mm 0.0075mm

15 minutes 0.015mm 0.015mm 0.0075mm

20 minutes 0.015mm 0.015mm 0.0075mm

25 minutes 0.015mm 0.015mm 0.0075mm

30 minutes 0.015mm 0.015mm 0.0075mm

35 minutes 0.015mm 0.015mm 0.0075mm

40 minutes 0.015mm 0.015mm 0.0075mm

45 minutes 0.01875mm 0.015mm 0.0075mm

50 minutes 0.01875mm 0.015mm 0.0075mm

55 minutes 0.01875mm 0.015mm 0.0075mm

60 minutes 0.01875mm 0.015mm 0.0075mm

SIZE OF RBC ACROSS

TIME

0

0.002

0.004

0.006

0.008

0.01

0.012

0.014

0.016

0.018

0.02

0.07 M

0.15 M

0.30 M

DISCUSSION

FACTORS AFFECTING

RESULT

NaCl solutions were not exactly

0.07M, 0.15M, and 0.30M

Unequal amount of NaCl solutions

and blood

DISCUSSION

A solvent always moves from a higher

concentration to a lower concentration which

equalizes the concentrations of solutes on both

sides.

In the experiment, the RBC’s immersed in

0.07 M sol’n became bloated or hemolyzed

0.15 M sol’n retained their shape and size

0.30 M sol’n shrunk or crenated

CONCLUSION

With the data acquired, we can say that,

A) In the 0.07 M slides, the solvent flowed from outside the RBC to inside the RBC.

Thus, the 0.07 M sol had a lower concentration of solutes than those of the RBC’s.

It is a hypotonic solution.

CONCLUSION

B) In the 0.15 M slides, the RBC’s showed

no signs of change.

Thus, 0.15 M sol had a similar or same

concentration of solutes like those of the

RBC’s.

It is a isotonic solution.

CONCLUSION

C) In the 0.30 M sol, the solvent flowed from

inside the RBC to outside the RBC.

Thus, the 0.30 M sol had a higher

concentration of solutes than those of the

RBC’s.

It is a hypertonic solution

REAL LIFE APPLICATION

REFERENCES

Campbell Biology, J. Reece et. al, (Tenth Edition)

Oxford Dictionary of English

Merriam-Webster Dictionary

Bettina Agnes Lee Josh Christian Protacio

Justin Stuart Tcheng Darla Teylan

OSMOSIS IN HUMAN RED BLOOD

CELLS