BiopsyBiospecimen

HistopathologicDiagnosis

morphology

cell type

Immunohisto-chemistry

FISH

differentiation

mitotic activity

tumor grade

Prognostic and Predictive Markers

Estrogen Receptor

ProgesteroneReceptor

HER2/neu

Ki-67

Perou et al., Nature 2000. PMID: 10963602Sorlie et al., Proc Natl Acad Sci 2001. PMID: 11553815

Parker et al., J Clin Oncol 2009. PMID: 19204204

PAM50: qRT-PCR assay for 50 genes to assign intrinsic subtypes using FFPE samples

Rosen’s Breast Pathology, 4th ed. Chapter 45.IHC: Immunohistochemistry

Tang et al., Arch Pathol Lab med 2016. PMID: 27472239IHC: Immunohistochemistry

Polley MYet al., JNCI 2013. PMID: 24203987 Elmore JG et al., JAMA 2015. PMID: 25781441

HistopathologicDiagnosis

morphology

cell type

Immunohisto-chemistry

FISH

differentiation

mitotic activity

tumor grade

Diagnostic BiopsyBiospecimen

Prognostic and Predictive Markers

Estrogen Receptor

ProgesteroneReceptor

HER2/neu

Ki-67

Molecular Analyses

Microarray / qRT-PCR

WGS / WES

RNA-sequencing

Next-Generation Sequencing (NGS) allows us to detect all classes of genomic alterations

Siimon R et al. Nature Reviews Drug Discovery. 2013. PMID: 23629504

TCGA., Nature 2012. PMID: 23000897

Burrell RA et al., Nature 2013. PMID: 24048066Burrell RA et al., Nature 2013. PMID: 24048066

Chung et al. In Revision

Carter et al., Nature Biotechnology 2012.

Roth et al., Nature Methods 2014.

Jiao et al., BMC Bioinformatics 2014.

Gerlinger et al., N Eng J Med 2012.Zhang et al., Science 2014.Bruin et al., Science 2014.

low throughput

low sensitivity

Navin NE, Genome Res. 2015. PMID: 26430160Wang Y & Navin NE, Molecular Cell 2015. PMID 26000845

Navin NE., Genome Res. 2015. PMID: 26430160

Tissue

single-cell RNA-seq

ISHlandmark gene expression

reference database

Combined analysis

Spatial Resolution

Tissue

Single-cell or regionalGenomic Analysis

HistopathologicEvaluation

HistopathologicDiagnosis

morphology

cell type

Immunohisto-chemistry

FISH

differentiation

mitotic activity

tumor grade

Diagnostic BiopsyBiospecimen

Prognostic and Predictive Markers

Estrogen Receptor

ProgesteroneReceptor

HER2/neu

Ki-67

Molecular Analyses

Microarray / qRT-PCR

WGS / WES

RNA-sequencing

Single-cell or target regionsequencing

Wang Y & Navin NE, Molecular Cell 2015. PMID 26000845

Kim SS, Kwon SH, Han W, Lee HB. In submission.

breast cancer

leukemia

isolation of single cell from

immunofluorescent cells

s = subclonal frequency, n = number of single cells

SNU Lab of Breast Cancer Biology (LBCB)Dong-Young NohWonshik HanHyeong-Gon MoonMinju Lee

SNU Biophonics and Nano Engineering LabSunghoon KwonSung-Sik KimChungwon Lee

Celemics Inc.