Post on 12-Jan-2016
description
transcript
BSL3BSL3
To InfectTo Infect
InhalationInhalation
LaboratoryLaboratory
SystemSystem
AnimalsAnimals
Team MembersTeam MembersNick Anders – Team LeaderNick Anders – Team Leader
Stacey Hoebel – Stacey Hoebel – CommunicationsCommunications
Jeff Swift – BWIGJeff Swift – BWIG
Maja Middleton – BSACMaja Middleton – BSAC
Kayla Ericson – BWIGKayla Ericson – BWIG
Austin Ramme - BWIGAustin Ramme - BWIG
AdvisorAdvisorProfessor Willis TompkinsProfessor Willis Tompkins
ClientClientAdel TalaatAdel Talaat
Assistant ProfessorAssistant Professor
Department of Animal and Biomedical SciencesDepartment of Animal and Biomedical Sciences
AbstractAbstract
Our objective is to build a safe system for Our objective is to build a safe system for infecting small animals with a Biological infecting small animals with a Biological Safety Level 3 pathogen. The design must Safety Level 3 pathogen. The design must incorporate safety features to prevent the incorporate safety features to prevent the escape of the infectious materials from the escape of the infectious materials from the infection chamber to the surrounding infection chamber to the surrounding environment. The research will be used to environment. The research will be used to study pathogenesis of tuberculosis. study pathogenesis of tuberculosis. Ultimate outcome of this study is the Ultimate outcome of this study is the development of novel drugs and vaccines development of novel drugs and vaccines that can cure tuberculosis patients.that can cure tuberculosis patients.
Problem Problem StatementStatement
Develop a safe inhalation system for Develop a safe inhalation system for animalanimal
infectioninfectionShould be able to infect animals Should be able to infect animals ranging inranging in
size from mice, rats, and guinea pigs size from mice, rats, and guinea pigs to to
rabbitsrabbitsShould be equipped with pathogen Should be equipped with pathogen escapeescape
prevention measures to insure safety prevention measures to insure safety in thein the
surrounding environmentsurrounding environment
BackgroundBackground
Client’s goals of animal infection studyClient’s goals of animal infection study– To study the pathenogenesis of To study the pathenogenesis of
tuberculosis (BSL3)tuberculosis (BSL3)– To develop a cure for TB patientsTo develop a cure for TB patients
Safety PrecautionsSafety Precautions– Proper training and protective clothingProper training and protective clothing– Non-porous materialNon-porous material– Autoclave in work zoneAutoclave in work zone– HEPA filtrationHEPA filtration– Sealed exhaust system with directional Sealed exhaust system with directional
airflow airflow
Current Device in UseCurrent Device in Use
Main chamber: 75 cm x 50 cm x 15 cmMain chamber: 75 cm x 50 cm x 15 cm Inner chambers: radius 8 cm, height 12 cmInner chambers: radius 8 cm, height 12 cm Nebulizer, Ethanol Trap, HEPA FilterNebulizer, Ethanol Trap, HEPA Filter TupperwareTupperware
Current Models availableCurrent Models available•Glas-Col Manufactures a model for $15,000
•Independent system
•UV light and HEPA filters incorporated
•Automated controls
•UW-Madison Engineering Shops make a model for $33,000
•Independent system
•Stainless Steel Cylinder
•Automated Controls
•Clamps for seal
Design SpecificationsDesign Specifications Dimensions:Dimensions:
– Length: 25 cm – 75 cmLength: 25 cm – 75 cm– Width: 40 cm – 30 cmWidth: 40 cm – 30 cm– Height: 10 cm – 70 cmHeight: 10 cm – 70 cm
Withstand the Autoclaving ProcessWithstand the Autoclaving Process Storage at Room TemperatureStorage at Room Temperature Air Tight StructureAir Tight Structure Deliver 5 mL of culture in 20 minutesDeliver 5 mL of culture in 20 minutes Service Life: 50 to 100 UsesService Life: 50 to 100 Uses
Alternative Design 1:Alternative Design 1:
ProsPros– Dual Chamber Dual Chamber
SystemSystem– Split Air FlowSplit Air Flow– Latex Flange around Latex Flange around
NeckNeck ConsCons
– Specific only to miceSpecific only to mice– Too difficult to Too difficult to
manufacturemanufacture– Too expensiveToo expensive
Air input
animal
Air input
Animal head
Air output
Alternative Design 2:Alternative Design 2: ProsPros
– AutoclavableAutoclavable– Even pathogen deliveryEven pathogen delivery
Nose pieceNose piece Split TubingSplit Tubing
ConsCons– Too difficult to Too difficult to
manufacturemanufacture Nose pieceNose piece TubingTubing
– Too expensive Too expensive – Infects only 4 animals Infects only 4 animals
at onceat once
Alternative Design 3:•Pros
•Jars for various animal sizes
•Latex diaphragms for restraint
•Circulation fan for distribution
•Fits under fume hood
•Cons
•Too difficult to manufacture
•Too expensive
•Lid with Gasket Tape and Output Valve
•PVC Pipe Stand
•Stainless Steel Mesh Shelf
•Input Valve with Elbow for Air Flow
•Latches
System AdvantagesSystem Advantages
Fully AutoclavableFully Autoclavable
Air Tight SystemAir Tight System– Increased EfficiencyIncreased Efficiency– Increased SafetyIncreased Safety
Even Distribution of PathogenEven Distribution of Pathogen
Increased Holding Container Increased Holding Container SizeSize– Small Animals to Large AnimalsSmall Animals to Large Animals– Greater Numbers per TrialGreater Numbers per Trial
Manufacturing: Clamp Manufacturing: Clamp AttachmentAttachment
Holes Drilled at Holes Drilled at Equal IntervalsEqual Intervals
Holes Filed and Holes Filed and Finished to Remove Finished to Remove Sharp EdgesSharp Edges
Teflon Gasket Teflon Gasket Custom Fit to Custom Fit to ScrewsScrews
Screws, Teflon Screws, Teflon Washer, Nut, and Washer, Nut, and Clamp AttachedClamp Attached
Attached Clamp
Teflon Washer
Clamp AssemblyNut
Screw
Manufacturing: LidManufacturing: Lid
LidLid– 3 layers of PTFE gasket tape on inside 3 layers of PTFE gasket tape on inside
liplip– Creates seal between pot and lidCreates seal between pot and lid
Manufacturing: InflowManufacturing: Inflow
½” Diameter Hole ½” Diameter Hole Drilled with Step Drilled with Step DrillDrill
Elbow Positioned Elbow Positioned on Threaded End of on Threaded End of Hose Nipple Hose Nipple
Hose Nipple Hose Nipple Welded to PotWelded to Pot
Elbow positioned to Elbow positioned to 4545oo to promote air to promote air circulationcirculation
Inflow Elbow
Inflow Hose Nipple
Manufacturing: OutflowManufacturing: Outflow
½” Diameter Hole ½” Diameter Hole Drilled with Step Drilled with Step DrillDrill
Hose Nipple Hose Nipple Welded to PotWelded to Pot
Outflow Hose Nipple
CostCost 48 qt. Stainless Steel Stockpot 48 qt. Stainless Steel Stockpot
$90.00$90.00 Draw Latches (8)Draw Latches (8) $33.40$33.40 Woven Wire ClothWoven Wire Cloth $33.00$33.00 Stainless Hose Adapters (2)Stainless Hose Adapters (2) $16.50$16.50 4545oo Elbow Fitting Elbow Fitting $11.60$11.60 PTFE Gasket TapePTFE Gasket Tape $32.50$32.50 Welding and Labor Welding and Labor $50.00$50.00
TOTALTOTAL $267.00$267.00
Design MatrixDesign MatrixDesign1Design1 Design2Design2 Design3Design3 FinalFinal
CostCost 4 4 3 3 2 2 9 9
Ease of Ease of 5 5 6 6 3 3 7 7
ManufactureManufacture
CleaningCleaning 5 5 4 4 5 5 8 8
SafetySafety 7 7 6 6 7 7 7 7
Overall Overall 2121 1919 1717 31 31
FUTURE WORKFUTURE WORKTESTINGTESTING
–Test with water and non-pathenogenic Test with water and non-pathenogenic bacteriabacteria
EfficiencyEfficiencyConsistencyConsistencySafetySafetyAir FlowAir Flow
Improved animal holding chambersImproved animal holding chambers–Nose/Head onlyNose/Head only
ReferencesReferences
www.McMaster-Carr.comwww.McMaster-Carr.com
www.EastmanOutdoors.comwww.EastmanOutdoors.com
www.glascol.com/products/biotimag/biotech.htmlwww.glascol.com/products/biotimag/biotech.html
www.hc-sc.gc.ca/pphb-dgspsp/publicat/lbg-ldmbl-www.hc-sc.gc.ca/pphb-dgspsp/publicat/lbg-ldmbl-
96/96/
www.nalgene.comwww.nalgene.com
Special ThanksSpecial Thanks
Professor Tompkins Professor Tompkins (Advisor)(Advisor)
Orrie Lokken (ME Orrie Lokken (ME Shop Supervisor)Shop Supervisor)
Adel Talaat (Client)Adel Talaat (Client)
Nick Anders (our Nick Anders (our fallen leader)fallen leader)