Carl Henrik Gørbitz, Ute KrengelDepartment of Chemistry, UiO

Crystal engineering

Protein crystallography

Biomarker identification

Molecules

H2

ethanesucrose

Molecules

Molecules

Crystal

Crystal

”Crystal engineering”, we make a supermolecule!

Building blocks

nodelinker

Problem 1

Molecules are very small, hard to

put together one by one

Xe-atoms on Ni-surface (Don Eigler, 1989)

Problem 2

As they are so small, we must put together very many before we reach a macroscopic size

A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules

Building blocks

Building blocks

Glue

Intermolecular forces

Must be: strong Directional

Two main types used in CE: Hydrogen bonds Metal coordination

MOFs

“Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous” (from Wikipedia)

MOF-5

Can we do this without metal ions?

Guanidinium derivatives

Fumaric acid complex

CN

N

NH

H

H

H

H

H

+

Guanidinium derivatives

DNA-based systems

DNA-based systems

DNA-based systems

Applications

Construction of polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more

Biomarker identification

Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule

Present in small amounts = hard to identify

Biomarker identification

Nano-HPLC-system

Group

Carl Henrik

Fassil

Lianglin

Malgorzata

Lise-Lotte

StevenOla

Protein crystallography

Structure/Function Studies of Structure/Function Studies of Medically Relevant SystemsMedically Relevant Systems

Department of Chemistry

Ute Krengel

University of Oslo

X-ray crystallography

Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)

MethodsMethods

Glycobiological Targets◦Mucins◦Bacterial Toxins◦Bacterial Adhesins◦Anti-tumor Antibodies◦Mushroom Lectins

Enzymes◦Chorismate mutases

GlycoNor

TargetsTargetsProtStru

ct

Glycobiological Targets:◦Bacterial Toxins – Structure and Function

Investigation of blood group dependence Delivery mechanisms? Drug design

◦Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray

structure determination

Masters ProjectsMasters Projects

GroupGroup

Ute

Øyvind

Hedda

DanielJulie

Dipankar

Dani