Post on 19-Feb-2018
transcript
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Acetic Acid 2 *
N -Acetyl-2,3-didehydro-2-deoxyneuraminic Acid
39 *
N -Acetylneuraminic Acid 1, 39 * * *
N -Acetylneuraminic AcidMethyl Ester
39 *
N -Acetylserotonin 48 *
Adenosine Diphosphate(ADP)
4, 5, 49 * * *
Adenosine Monophosphate(AMP)
4, 5, 6,49
* * *
Adenosine Triphosphate(ATP)
4, 5, 49 * * *
Adrenaline 8 * * *
Alanine 9 *
Allantoin 10 * *
Alprenolol 11,12,13 * * *
-Aminobutyric Acid(GABA)
14 * * *
4-(Aminomethyl)benzoic Acid 42 *
Arginine 9 *
Chemical Name Structural Formula Page.
Cation-Exchange
A
1 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
L-Ascorbic Acid(Vitamin C)
2, 3, 41,51
* * *
Aspartic Acid 9 *
Benzene 15, 16, 17 * * *
Benzenesulfonic Acid 15, 16, 17 * * *
Benzoic Acid 40, 51 *
Benzonitrile 18 * * *
Bis[(trifluoromethyl)sulfonyl]amine Anion
(CF3SO2)2N- 37 *
Buformin 38 *
1-Butyl-3-methylimidazoliumChloride
34 *
Caffeine 19 * * *
Chlorpromazine 13 *
Citric Acid 2 *
Clomipramine 13 *
Creatine 43 *
Creatinine 8 * * *
A
B
C
2 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
4-Cyanobenzoic Acid 42 *
Cyclic AdenosineMonophosphate (cAMP)
4, 49 * * *
Cytidine Monophosphate(CMP)
6, 7 * * *
Dehydroacetic Acid 51 *
Dichloroacetic Acid 20 *
Dicyanamide Anion (NC)2N- 36 *
3,5-Dihydroxybenzoic Acid 18 * * *
3,5-Diiodo-L-thyronine 50 *
N ,N -Dimethylsulfamide 52 *
DOPA 8 * * *
Dopamine 8,18 * * *
Erythorbic Acid 41 * * *
Ethylbenzene 15, 16, 17 * * *
4-Ethylbenzene sulfonic Acid 15, 16, 17 * * *
2-Ethyl-4-methylimidazole 21 *
D
E
C
S
O
N NH2
O
3 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
E 1-Ethyl-3-methylimidazolium 36, 37 *
F Fumaric Acid 45 *
GABASee Page.1
"-Aminobutyric Acid"14
Galacturonic Acid 2,3 * * *
2-O-α-D-Glucopyranosyl-L-ascorbic Acid
41 * * *
Glucose 1-Phosphate 47 *
D-Glucose 6-Phosphate 47 *
L-Glutamic Acid 14 * * *
Glutathione oxidized form(GSSG)
44 *
Glutathione reduced form(GSH)
44 *
N -Glycolylneuramic Acid 1, 39 * * *
Glyphosate 22 * * *
Guanosine Monophosphate(GMP)
6 * *
H Hexafluorophosphate PF6- 37 *
G
4 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
Histamine 23, 24 * *
Homovanillic Acid(HVA)
8 * * *
3-Hydrazinobenzoic Acid 40 *
4-Hydrazinobenzoic Acid 40 *
5-Hydroxyindole-3-aceticAcid
48 *
5-Hydroxy-L-tryptophan 25, 48 * * *
Inosine Monophosphate(IMP)
6 * *
Isobutyl 4-Hydroxybenzoate 27 *
Isoproterenol 8 * * *
Lactic Acid 2 *
Levulinic Acid 28 *
Lithium Chloride LiCl 33 *
Maleic Acid 45 *
Malic Acid 2, 45 *
Melamine 29 * * *
Melatonin 48 *
L
M
H
I
5 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
Metformin 26, 27, 38 * * *
Methionine 9 * *
Methyl Levulinate 28 *
4-Methyl-1H -benzotriazole 46 *
5-Methyl-1H -benzotriazole 46 *
2-Methylimidazole 21 *
Monochloroacetic Acid 20 *
NAD+(Nicotinamide AdenineDinucleotide)
30 * *
Nicardipine 11, 12, 13 * * *
Nicotinic Acid 30 * *
Noradrenaline 8 * * *
D-Panthenol 10 * *
Phenformin 38 *
2-Phenoxyethanol 51 *
P
N
M
6 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
Phenylalanine 9 *
L-Phenylephrine 19 * * *
Phosphocreatine 43 *
Potassium Bromide KBr 32, 35 *
Potassium Chloride KCl 32, 33 * *
Potassium Iodide Kl 32, 35 *
Serotonin (5-HT) 8, 48 * *
Sodium Chloride NaCl 33 *
Sorbic Acid 51 *
Succinic Acid 45 *
Tartaric Acid 45 *
Tetrafluoroborate Anion BF4- 36 *
DL-Thyronine 50 *
L-Thyroxine (T4) 50 *
Toluene 15, 16, 17 * *
p -Toluenesulfonic Acid 15, 16, 17 * * *
P
T
S
7 / 8
Anion-Exchange
ODS-CX10
ODS-CX15
ODS-CX20
ODS-AX20
ODS-AX10
ODS-SAXSuper
Chemical Name Structural Formula Page.
Cation-Exchange
Toluidine (o ,m ,p ) 31 * * *
Tranexamic Acid 42 *
Trichloroacetic Acid 20 *
Trifluoromethanesulfonate CF3SO3- 36 *
(Trifluoromethyl)trifluoroborateAnion CF3BF3
- 36, 37 *
3,3',5-Triiodo-L-thyronine (T3) 50 *
L-Tryptophan 48 *
Tyramine 8 * * *
UUridine Monophosphate(UMP)
6,7 * * *
Vanillylmandelic Acid(VMA)
8 * * *
Verapamil 11, 12, 13 * * *
Vitamin CSee Page.1
"L-Ascorbic Acid"2, 3, 41,
51
T
V
8 / 8
-1-
Sialic Acids
Column : Kaseisorb LC ODS-SAX Super Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20 Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 5 / 95
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. N-Glycolylneuramic acid
2. N-Acetylneuramic acid
O
OH
COOH
HO
HN
HO OH
HO
HOH2C
O
O
OH
COOH
HO
HN
HO OH
HO
O
0.0 2.5 5.0 7.5 10.0 12.5 min
1
1
1
1
2
2
2
2
-2-
Organic Acids
Sample : Galacturonic Acid
Sample : Ascorbic Acid
Sample : Lactic Acid
Sample : Acetic Acid
Sample : Malic Acid
Sample : Citric Acid
Column TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH7.0, H3PO4) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
Galacturonic Acid
-Ascorbic Acid (Vitamin C)
Lactic Acid
Acetic Acid
Malic Acid
Citric Acid
OH
OH
O
OH
OHO
OH
OHO
O
O
OH OH
O
OH
O
OH
OH OH
O OHO
OH
O
OH
HO
COOH
OH
HO
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 min
-3-
Organic Acids
Column : Kaseisorb LC ODS-SAX Super Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20 Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH7.0, H3PO4) = 10 / 90
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Galacturonic Acid
2. -Ascorbic Acid (Vitamin C)
0.0 2.5 5.0 7.5 min
1
1
1
1,2
2
2
2
OH OH
O OHO
OH
O
OH
HO
COOH
OH
HO
-4-
Adenosine Phosphates
Column: Kaseisorb LC ODS-SAX Super
Column: TCI Dual ODS-AX10
Column: TCI Dual ODS-AX20
Column: Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A : CH3OH / 10 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80
B : CH3OH / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80
0-100 % B (10-30 min)
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. AMP 3. ADP
2. cAMP 4. ATP
N
NN
N
NH2
OH OH
OOP
O
HO
OH
N
NN
N
NH2
O OH
OO
PO
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PHO
O
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PO
O
OH
PHO
O
OH
1
0 10 20 30 40 min
1
1
1
2
2
2
2
3
3
3
4,3
4
4
4
-5-
Nucleotides (Adenosine Phosphates)
Column: Kaseisorb LC ODS-SAX Super
Column: TCI Dual ODS-AX10
Column: TCI Dual ODS-AX20
Column: Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 20 mmol/L NH4HCO3 (pH 7.8) = 20 / 80
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. AMP 3. ATP
2. ADP
N
NN
N
NH2
OH OH
OOP
O
HO
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PHO
O
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PO
O
OH
PHO
O
OH
ADP and ATP is not elution.
0.0 5.0 10.0 15.0 20.0 25.0 min
1
1
1
1,2,3
2
2
3
3
-6-
Nucleotides Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH 7.0,H3PO4) = 20 / 80
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. CMP
2. UMP
3. IMP
4. GMP 5. AMP
0 5 10 15 (min)
1
2
3,4
5
1 2
3
4
5
0 5 10 15 (min)
N
NH2
ON
O
OHOH
OPHO
OH
O
NH
O
ON
O
OHOH
OPHO
OH
O
N
NHN
N
O
OH OH
OOP
O
HO
OH
N
NHN
N
O
OH OH
OOP
O
HO
OH
NH2N
NN
N
NH2
OH OH
OOP
O
HO
OH
-7-
Nucleotides
Column : Kaseisorb LC ODS-SAX Super
Column : TCI Dual ODS-AX10
Column : TCI Dual ODS-AX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH 7.0, H3PO4) = 10 / 90
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. CMP (Cytidine Monophosphate)
2. UMP (Uridine Monophosphate)
N
NH2
ON
O
OHOH
OPHO
OH
O
NH
O
ON
O
OHOH
OPHO
OH
O
0.0 2.5 5.0 7.5 10.0 12.5 min
1
1,2
1
1,2
2
2
-8-
Catecholamines
Column: TCI Dual ODS-CX20
Column: TCI Dual ODS-CX15
Column: TCI Dual ODS-CX10
Column Size 4.6 mmI.D. × 150 mm
Mobile Phase CH3CN / 50 mmoL/L HCOONH4 (pH 3.5, HCOOH) = 70 / 30
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 25 ℃
Sample
1. VMA
2. DOPA
3. HVA
4. Noradrenaline
5. Adrenaline
6. Creatinine
7. Dopamine
8. Serotonin
9. Tyramine
10. Isoproterenol
OH
O
HOOH
O
NH2
OH
OHO
OH
O
OH
OH
O
OH
OH
NH2
HO
OH
OH
HONH
NHN
O
NH
OH
OH
NH2
NH
HO
NH2
OH
NH2
OH
HN
HO
OH
2
2
2,4,5,78,9,10
3
3
3 4
4
5
5
1
1
1 6
6
7
7
8
8 9
9 10
10
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
-9-
Amino Acids
Sample : Aspartic Acid
Sample : Alanine
Sample : Methionine
Sample : Phenylalanine
Sample : Arginine
Column TCI Dual ODS-CX15
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A: CH3CN / 5 mmol/L KH2PO4 (pH 3.0, H3PO4) = 5 / 95
B: CH3CN / 50 mmol/L KH2PO4 (pH 3.0, H3PO4) = 40 / 60
0-100 % B (5-20 min)
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
Aspartic Acid
Alanine
Methionine
Phenylalanine
Arginine
HOOH
NH2
O
O
OH
O
NH2
SOH
O
NH2
OH
NH2
O
H2N NH
OH
NH O
NH2
0.0 5.0 10.0 15.0 20.0 25.0 min
-10-
Allantoin, D-Panthenol
Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 20 mmol/L K2HPO4 (pH 6.0, H3PO4) = 10 / 90
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. Allantoin
2. D-Panthenol
0 4 8 12 (min)
0 4 8 12 (min)
NH
NH
NH
O
H2N O
O
HO NH
O
OH
1
1
2
2
-11-
Basic Drugs
Column: TCI Dual ODS-CX10
Column: TCI Dual ODS-CX15
Column: TCI Dual ODS-CX20
Column: Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 3.5, CH3COOH) = 80 / 20
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Nicardipine
2. Verapamil
3. Alprenolol
0 25 50 75 100 min
1
1
1
1
2
2
2
23
3
3
3
N
O
O
NO2
O
ON
OHN
OH
-12-
Basic Drugs
Column TCI Dual ODS-CX10
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 80 / 20
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Nicardipine
2. Verapamil
3. Alprenolol
OHN
OH
N
O
O
NO2
O
ON
0.0 25.0 50.0 75.0 100.0 min
1
2
3
-13-
Basic Drugs
Column TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN/ 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 80 / 20
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Nicardipine
2. Verapamil
3. Alprenolol
4. Clomipramine
5. Chlorpromazine
OHN
OH
N
N
Cl
S
N
N
Cl
N
O
O
NO2
O
ON
0 10 20 30 40 min
1
2
3
4
5
-14-
L-Glutamic Acid, GABA
Column : TCI Dual ODS-CX10
Column : TCI Dual ODS-CX15
Column : TCI Dual ODS-CX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / H2O / H3PO4 = 5 / 95 / 0.1
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 25 ℃
Sample
1. L-Glutamic Acid
2. GABA (-Aminobutyric Acid)
H2NOH
O
HO OH
O O
NH2
0 10 20 30 40 50 min
1
1
1
1
2
2
2
2
-15-
0 10 20 30 40 50 60 70 80 min
1
1
1
2
2
2,6
3
3
3
4
4
4
5
5
5
6
6
Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50 Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50 Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50
Column TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 30 ℃
Sample
1. Benzene
2. Toluene
3. Ethylbenzene
4. Benzenesulfonic Acid 5. p-Toluenesulfonic Acid
6. 4-Ethylbenzenesulfonic Acid
SO3HSO3H
SO3H
-16-
Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50
Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50
Column TCI Dual ODS-AX10
Column Size 4.6 mmI.D. X 150 mm
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 30 ℃
Sample
1. Benzene
2. Toluene
3. Ethylbenzene
4. Benzenesulfonic Acid 5. p-Toluenesulfonic Acid
6. 4-Ethylbenzenesulfonic Acid
SO3HSO3H
SO3H
0.0 5.0 10.0 15.0 20.0 25.0 min
1
1
1
2
2
2
3
3
3
4
4
4
5
5
5
6
6
6
-17-
0.0 5.0 10.0 15.0 20.0 25.0 min
1
1
1
2
2
2
3
3
3
4
4
4
5
5
5
6
6
6
Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50
Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50
Column Kaseisorb LC ODS-SAX Super
Column Size 4.6 mmI.D. X 150 mm
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 30 ℃
Sample
1. Benzene
2. Toluene
3. Ethylbenzene
4. Benzenesulfonic Acid 5. p-Toluenesulfonic Acid
6. 4-Ethylbenzenesulfonic Acid
SO3HSO3H
SO3H
-18-
Column : TCI Dual ODS-AX20
Column : TCI Dual ODS-AX10
Column : Kaseisorb LC ODS-SAX Super
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 30 mol/L CH3COONH4 (pH 4.5, CH3COOH) = 40 / 60
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. Dopamine
2. Benzonitrile
3. 3,5-Dihydroxybenzoic Acid
HO
HO
NH2 CN
OHHO
C
O
OH
0.0 2.5 5.0 7.5 10.0 12.5 min
1
1
1
2
2
2
3
3
3
-19-
Column : TCI Dual ODS-CX10
Column : TCI Dual ODS-CX15
Column : TCI Dual ODS-CX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / H2O / TFA = 20 / 80 / 0.05
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Caffeine
2. L-Phenylephrine
N
N N
N
O
O
NH
OH
HO
H
L-Phenylephrine is not elution.
0.0 5.0 10.0 15.0 min
1
1
1
1
2
2
2
-20-
0.0 5.0 10.0 15.0 min
0
10
20
30
40
50
60
70
mAU
Halogenated Acetic Acids
Column Kaseisorb LC ODS-SAX Super
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 25 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. Monochloroacetic Acid
2. Dichloroacetic Acid
3. Trichloroacetic Acid
1
3
2
OH
O
Cl ClOH
O
Cl
ClOH
O
ClCl
-21-
0.0 5.0 10.0 15.0 min
0
100
200
300
400
500
600
700
mAU
Imidazoles
Column TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. 2-Methylimidazole 2. 2-Ethyl-4-methylimidazole
1
2
N
NH
NH
N
-22-
Herbicide
Column : Kaseisorb LC ODS-SAX Super
Column : TCI Dual ODS-AX10
Column : TCI Dual ODS-AX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 5 mmol/L KH2PO4 (pH 3.0, H3PO4) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
Glyphosate
HO
HN P
OH
O O
OH
0 10 20 30 40 50 min
-23-
Histamine
Column TCI Dual ODS-CX10
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 100 mmol/L CH3COONH4 = 50 / 50
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
Histamine
N
NH
H2N
-24-
Histamine
Column TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 100 mmol/L CH3COONH4 = 50 / 50
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
Histamine
N
NH
H2N
-25-
5-Hydroxy-L-tryptophan
Column : TCI Dual ODS-CX10
Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 10 / 90
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
5-Hydroxy-L-tryptophan
0.0 2.5 5.0 7.5 10.0 12.5 min
-26-
Metformin
Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20 Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L KH2PO4 (pH 3.0, H3PO4) = 50 / 50
Detection UV 235 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
Metformin
N NH
NH2
NH NH
0 10 20 30 40 50 min
-27-
Metformin and Isobutyl 4-Hydroxybenzoate
Mobile Phase : CH3CN / 50 mmol/L KH2PO4
(pH 3.0, H3PO4) = 50 / 50
Mobile Phase : CH3CN / 50 mmol/L HCOONH4
(pH 3.0, HCOOH) = 50 / 50
Column TCI Dual ODS-CX15
Column Size 4.6 mmI.D. X 150 mm
Detection UV 235 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Isobutyl 4-Hydroxybenzoate
2. Metformin
0.0 5.0 10.0 15.0 20.0 25.0 min
1
1
2
2
HO
O
O
N NH
NH2
NH NH
-28-
0.0 2.5 5.0 7.5 min
0
10
20
30
40
50
60
70
80
90
mV
2.742
3.729
Levulinic Acid, Methyl Levulinate
Column TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 20 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
1. Levulinic Acid
2. Methyl Levulinate
1
2
OH
O
O
-29-
Melamine
Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 20 mmol/L KH2PO4 (pH 3.0, H3PO4) = 40 / 60
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
Melamine
NN
N
NH2
NH2H2N
0 10 20 30 40 50 min
-30-
N+
OH OH
OOP
O
NH2
O
P N
OH OH
ON
NN
NH2
O
O-
O-
O
Nicotinic Acid and Derivatives
Column : Kaseisorb LC ODS-SAX Super
Column : TCI Dual ODS-AX10
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 10 / 90
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. NAD+
2. Nicotinic Acid
0 5 10 min
1
1
2
2
N
OH
O
-31-
Toluidines
Column
A) TCI Dual ODS-CX10
B) TCI Dual ODS-CX15
C) TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L HCOONH4 (pH 3.4, HCOOH) = 50 / 50
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
1. o-Toluidine
2. m-Toluidine
3. p-Toluidine
A) TCI Dual ODS-CX10
C) TCI Dual ODS-CX20
B) TCI Dual ODS-CX15
NH2
NH2
NH2
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 min
1
1
1
2
2
2
3
3
3
-32-
3
min0 2.5 5 10 15 17.5 7.5 12.5
2
3
4
min 0 2 4 6 8 10 12 14 16 18
4
1
Halide Ions Detection : Corona CAD
Detection : UV 210 nm
Column Kaseisorb LC ODS-SAX Super
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Detection Corona CAD (Range: 100 pA, Filter: High, Nebulizer: 25 °C), UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. K+
2. Cl-
3. Br-
4. I-
-33-
Alkali Halides
Column TCI Dual ODS-CX15
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Detection Corona CAD (Range : 100 pA, Filter: High, Nebulizer: 25 °C)
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Cl-
2. Li+
3. Na+
4. K+
min0 2.5 5 7.5 10 12.5 15 17.5
1
3
2
4
-34-
Ionic liquid
Column TCI Dual ODS-CX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 10 / 90
Detection Corona CAD (Range: 100 pA, Filter: Corona, Nebulizer: 25 °C)
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1-Butyl-3-methylimidazolium chloride
N
+N
Cl-
min0 2 4 6 8
Cl-
N
+N
-35-
Halide Ions
Column Kaseisorb LC ODS-SAX Super
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Br-
2. I-
0.0 5.0 10.0 15.0 min
1 2
-36-
1-Ethyl-3-methylimidazolium salts 1
Column Kaseisorb LC ODS-SAX Super (5μm)
Colum Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 100 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50
Detection Corona CAD
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1-Ethyl-3-methylimidazolium
(NC)2N-
BF4-
CF3BF3-
CF3SO3-
150000
170000
0 2.5 5 7.5 10 12.5 min
N
N+
(NC)2N-
BF4-
CF3BF3-
CF3SO3-
N
N+
-37-
1-Ethyl-3-methylimidazolium salts 2
Column Kaseisorb LC ODS-SAX Super (5 m)
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 100 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 75 / 25
Detection Corona CAD
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1-Ethyl-3-methylimidazolium
CF3BF3-
(CF3SO2)2N-
PF6-
150000
170000
0 2.5 5 7.5 10 12.5 min
N
N+
CF3BF3-
(CF3SO2)2N-
PF6-
N
N+
-38-
N NH
NH2
NH NH
Antidiabetic Drugs
Column TCI Dual ODS-CX15 (5 m)
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3OH / 20 mmol/L CH3COONH4 (pH 6.78) = 70 / 30
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Metformin
2. Buformin
3. Phenformin
Sample Size 1.0 L (1.0 mg/mL)
150000
170000
0 5 10 15 20 25 min
1
2
3
H2N NH
NH
NH NH
H2N NH
NH
NH NH
-39-
Sialic Acids
Column : TCI Dual ODS-AX10 (5μm)
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 5 / 95
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. N-Acetylneuraminic Acid Methyl Ester
2. N-Glycolylneuramic Acid
3. N-Acetylneuraminic Acid Hydrate
4. N-Acetyl-2,3-didehydro-2-deoxyneuraminic Acid
1
←2,3,4
1
2 3
4
0 5 10 min
-40-
Hydrazinobenzoic Acids
Column : TCI Dual ODS-AX10 (5 m)
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 10 mmol/CH3COONH4 (pH 5.0,CH3COOH) = 20 / 80
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. 4-Hydrazinobenzoic Acid
2. 3-Hydrazinobenzoic Acid
3. Benzoic Acid
3
1
2
←1,2
3
0 min 5 10 15 20
-41-
L-Ascorbic Acid related compounds Column : TCI Dual ODS-AX10 (5 m) Column : TCI Dual ODS-AX20 (5 m)
Column : Kaseisorb LC ODS SAX Super (5 m)
Column : Kaseisorb LC ODS 2000
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 10 / 90
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. 2-O-α-D-Glucopyranosyl- L-ascorbic Acid
2. L-Ascorbic Acid
3. Erythorbic Acid
3
2
1
1
2
3
←3
2
1
min0 5 10
←2,3
1→
OH OH
O OHO
OH
-42-
Tranexamic Acid related compounds
Column TCI Dual ODS-CX15 (5 m)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 25 mmol/L HCOONH4 (pH 3.5, HCOOH) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
1. 4-Cyanobenzoic Acid
(0.01mg/1.0mL)
2. 4-(Aminomethyl)benzoic Acid (0.01mg/1.0mL)
3. Tranexamic Acid (1.0mg/1.0mL)
Sample Size 1.0L
0 5 10 min
1
2
3
-43-
Creatine related compounds
Column Kaseisorb LC ODS-SAX Super (5 m)
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 10 / 90
Detection UV 220 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Creatine
2. Phosphocreatine
Sample Size 1.0 L (10mg/mL)
0 5 10 15 min
1
2
-44-
Glutathiones
Column TCI Dual ODS-AX20 (5 m)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 6.8, CH3COOH) = 30 / 70
Detection UV 230 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Glutathione reduced form (GSH)
2. Glutathione oxidized form (GSSG)
150000
170000
0 5 10 15 20 25 min
1
2
-45-
Organic Acids (Dicarboxylic acids)
Column TCI Dual ODS-AX20 (3 m)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 10mmol/L KH2PO4 (pH 7.0, H3PO4) = 20 / 80
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. Tartaric Acid
2. L-Malic Acid
3. Succinic Acid 4. Fumaric Acid
5. Maleic Acid
0 2.5 5.0 7.5 min
1
2
34
5
-46-
Anti-corrosion agent
Column TCI Dual ODS-CX15 (3 m)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3CN / 20 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 10 / 90
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 30 °C
Sample
1. 4-Methyl-1H-benzotriazole
2. 5-Methyl-1H-benzotriazole
Sample Size 1.0 L(1.0mg/mL mobile phase)
1
2
0 10 20 30 40 min
Methyl-1H-benzotriazole(mixture)
5-Methyl-1H-benzotriazole
-47-
0 2.5 5.0 min
1
2
Sugar Phosphate
Column TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH 6.9, H3PO4) = 5 / 95
Detection RI
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. D-Glucose 6-Phosphate
2. Glucose 1-Phosphate
O
OH
OHO
HO
OHP
OO-
O-
-48-
Serotonin and Metabolites
Column TCI Dual ODS-CX10
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A: CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 10 / 90
B: CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 70 / 30
A to 100 % B in 60 min
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 25 °C
Sample
1. 5-Hydroxy-L-tryptophan
2. 5-Hydroxyindole-3-acetic Acid
3.N-Acetylserotonin
4. L-Tryptophan
5. Serotonin
6. Melatonin
NH
HO O
OH
HO
NH
HN
O
NH
NH2
OH
O
NH
HO NH2
0 5 10 15 min
1
2
3
45
6
-49-
Adenosine Phosphates
Column TCI Dual ODS-AX20
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A : CH3CN / 10 mmol/L NH4HCO3 (pH 7.8) = 10 / 90
B : CH3CN / 20 mmol/L NH4HCO3 (pH 7.8) = 30 / 70
0 %B(0-3 min), 0-100 % B (3-13 min), 100 %B(13-20 min)
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. cAMP
2. AMP
3. ADP
4. ATP
0 5 10 15 min
2
1
3
4
N
NN
N
NH2
O OH
OO
PO
OH
N
NN
N
NH2
OH OH
OOP
O
HO
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PHO
O
OH
N
NN
N
NH2
OH OH
OOP
O
O
OH
PO
O
OH
PHO
O
OH
-50-
Thyroid hormones
Column TCI Dual ODS-CX15 (5μm)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A:CH3CN
B:20 mmol/L HCOONH4(pH 3.0, HCOOH)
80-40%B (0-8min), 40%B (8-15min)
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. DL-Thyronine
2. 3,5-Diiodo-L-thyronine
3. 3,3',5-Triiodo-L-thyronine (T3) 4. L-Thyroxine (T4)
0 5 10 min
1
2
3
4
-51-
Food additives
Column TCI Dual ODS-AX10 (5μm)
Column Size 4.6 mmI.D. X 150 mm
Mobile Phase
A:CH3CN / 5 mmoL/L CH3COONH4 = 5 / 95
B:CH3CN / 50 mmoL/L CH3COONH4 = 70 / 30
20-100%B (0-5min), 100%B (5-20min)
Detection UV 254 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
1. L-Ascorbic Acid (Vitamin C)
2. 2-Phenoxyethanol
3. Dehydroacetic Acid 4. Sorbic Acid
5. Benzoic Acid
0 5 10 15 min
2
1
3
4
5
-52-
N,N-Dimethylsulfamide
Column TCI Dual ODS-CX15 (5μm)
Column Size 4.6 mmI.D. X 50 mm
Mobile Phase CH3OH / 20 mmol/L CH3COONH4 (pH 6.88) = 2 / 98
Detection UV 210 nm
Flow Rate 1.0 mL/min
Temperature 40 °C
Sample
0 5 10 min
S
O
N NH2
O