Cellulase enzyme

EC Code =3.2.1.4Other name s: Avicelase- BETA-1,4-Endoglucan

Hydrolase - BETA-1,4Glucanase- Cello dextrinase Carboxy methyl cellulase ENDO-1,4-BETA-D-Glucanase-ENDO-1,4-BETA-D-Glucanohydrolase- ENDO-1,4-BETA-Glucanase- Endo glucanase -Alkali cellulase- Pancellasess

Systematic name:1,4-(1,3:1,4)-beta-D-glucan,4-glucano hydrolase

Introduction

1-4-BETA-GLUCOSIDIC bond hydrolysis in cellulose,…

REACTION

Endo-cellulaseExo-cellulasebeta-glucosidaseOxidative cellulaseCellulose phosphorylase

cellulases

BacteriaFungiActinomyceteProtozoaSome insects that digest woodSome animals & plantsNot mammal

Cellulase sources

DialysisFiltration gelAnionic resin exchange columnUsing aceton

Extraction

Cellulase production

Spectorophotometeryi. Reduced sugarii. Substrate decrease

Solid surface i. Propagation in substrateii. Electrophoreses gel

Cellulase activity assay

Food industryAgricultural industryPaper making industryWaste water treatmentTextile industry

Cellulase application

Enzymic hydrolysis rate

Cellulase action mechanism

Cellulase structure

The crystal structure of the catalytic domain of CelCCA

β strands and * helices in CelCCA.

Topological diagram of the secondary structural elements of CelCCA. helices are shown as cylinders and β strands as arrows.

Close up view of catalitic C-terminal extremlybof the barrel strands showing the arrangment of the 7 residues(red and yellow) along with the try (purple) at the intrancethe catalitic residues are red

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