Post on 12-Jun-2020
transcript
Disease update 2018
Sabine Banniza
CROP DEVELOPMENT CENTRE
The year that was …..
Early and late season precipitation in SK Based on SK MoA Crop Reports
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Aril - June July - October
* Estimated from Saskatchewan Research Council Graph
2017 Seed Quality Chickpea Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen
Ascochyta Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean % 1A 1 100 0 100 0 100 0 1B 0 nd nd nd nd nd nd 2A 18 100 0 100 0 100 0 2B 15 86.7 1.8 100 0 100 0 3AN 7 100 0 100 0 100 0 3AS 53 98.1 0.3 100 0 100 0 3BN 1 100 0 100 0 100 0 3BS 4 100 0 100 0 100 0 4A 0 nd nd nd nd nd nd 4B 0 nd nd nd nd nd nd 5A 0 nd nd nd nd nd nd 5B 0 nd nd nd nd nd nd 6A 0 nd nd nd nd nd nd 6B 4 100 0 100 0 100 0 7A 0 nd nd nd nd nd nd 7B 0 nd nd nd nd nd nd 8A 0 nd nd nd nd nd nd 8B 0 nd nd nd nd nd nd 9A 0 nd nd nd nd nd nd 9B 0 nd nd nd nd nd nd Total/Mean 103 94.3 1.3 100 0 100 0
2017 Seed Quality Lentil Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen
Ascochyta Anthracnose Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean % % PFS Mean %
1A 7 100 0 100 0 85.7 0.5 100 0 1B 2 100 0 100 0 100 0 100 0 2A 44 100 0 100 0 100 0 100 0 2B 92 98.9 1 100 0 96.7 1.4 98.9 2
3AN 18 100 0 100 0 100 0 94.4 0.3 3AS 93 100 0 98.9 0.3 93.5 2.2 94.6 1.3 3BN 63 100 0 98.4 0.8 93.7 0.8 95.2 0.5 3BS 16 100 0 100 0 100 0 100 0 4A 4 100 0 100 0 100 0 100 0 4B 7 100 0 100 0 100 0 100 0 5A 12 100 0 100 0 100 0 100 0 5B 0 nd nd nd nd nd nd nd nd 6A 37 100 0 91.9 0.6 81.1 0.8 91.9 0.5 6B 87 97.7 0.3 97.7 0.9 88.5 0.5 95.4 0.5 7A 79 98.7 0.3 89.9 0.6 83.5 0.8 91.1 0.5 7B 12 100 0 100 0 100 0 88.9 0.3 8A 1 100 0 100 0 100 0 100 0 8B 2 100 0 100 0 100 0 100 0 9A 1 100 0 100 0 100 0 100 0 9B 2 100 0 100 0 100 0 100 0
Total/Mean 579 99.3 0.5 95.7 0.7 92 0.9 95.7 0.7
2017 Seed Quality Pea Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen
Ascochyta Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean %
1A 14 100 0 100 0 100 0 1B 10 80 0.5 100 0 100 0 2A 9 100 0 100 0 87.5 1.5 2B 39 92.3 0.5 100 0 100 0
3AN 5 100 0 100 0 100 0 3AS 27 88.9 0.5 100 0 100 0 3BN 17 64.7 1 94.1 0.5 100 0 3BS 2 100 0 100 0 100 0 4A 3 33.3 4.8 66.7 1.5 100 0 4B 7 85.7 1.5 100 0 100 0 5A 12 91.7 2 100 0 100 0 5B 12 75 0.7 72.7 0.5 100 0 6A 35 85.7 0.5 100 0 100 0 6B 91 70.3 0.8 100 0 100 0 7A 29 62.1 1.7 96.6 0.5 96.6 0.5 7B 30 53.3 0.8 85.7 0.5 96.4 0.5 8A 10 70 0.6 90 0.5 100 0 8B 31 80.6 1.5 96.8 0.5 100 0 9A 21 57.1 1.4 85 0.6 95 0.5 9B 49 20.4 2.9 20.6 0.6 100 0
Total/Mean 453 70.2 1.6 90 0.6 99.1 0.8
Root rot incidence and severity S. Chatterton, M. Harding, R. Bowness, C. Vucurevich, T. Dubitz, J. Nielsen, B. Gossen,
S. Banniza, B. Ziesman, D. McLaren
No. fields surveyed
Root rot prevalence
(%)
Root rot incidence
Root rot incidence
> 3 (%)
Average severity
Pea east-central AB 35 100 91 36 3.3 west-central AB 89 100 73 31 2.8 southern AB 35 85 54 19 2.2 Alberta (Total) 159 97 73 30 2.8 Saskatchewan 100 100 93 43 3.5 Manitoba 31 100 97 36 3.3
Lentils southern AB 15 100 80 3.7 2.1 Saskatchewan 83 100 78 9.5 2.2
Dry bean southern AB 15 40 40 0 1.5
Aphanomyces Root Rot: Infectivity model for Aphanomyces euteiches in Saskatchewan soils
Syama Chatterton, AAFC Lethbridge
With how many Aphanomyces spore can a pea plant happily live?
Molecular quantification
Disease severity Correlation
Aphanomyces Root Rot: Infectivity model for Aphanomyces euteiches in
Saskatchewan soils Syama Chatterton, AAFC Lethbridge
Research team: Syama Chatterton (AAFC Lethbridge)
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Field Low Medium High 0-20cm GH-CtrlST 3.3 1MC 2.7 1ML 3.4 1EL 4.4 1SV 4.8 1GL 3.0 1LA 1.9 1MJ 3.3 1Ed` 1.6 1MS 3.2 1Ws 1.9 1
Disease Severity Disease Rating
2017 Field testing
Southy (Dark Brown) Maple Creek (Brown) Meadow Lake (Grey) Eldersley (Grey) Slyvania (Grey) Gull Lake (Brown) Lafleche (Brown) Moose Jaw (Dark Brown) Raymore (Black) Maidstone (Black) Watrous (Dark Brown)
DEVELOPING PEAS WITH BETTER APHANOMYCES
Lavaud et al 2015. Theor Appl Genet 128(11),2273-88
French pea map with Aphanomyces resistance QTLs
Lavaud et al 2015. Theor Appl Genet 128(11),2273-88
Developing peas with better Aphanomyces
• Crossing with – USDA material with
partial resistance (QTL Ae-Ps7.6)
– 90-2079 with partial resistance (QTL Ae-Ps4.5)
Parent 1 Parent 2 CDC 2847-21 /PI 660733 CDC 2847-21 CDC 2936-7 CDC 2950-19/PI 660736 CDC 2950-19/PI 660736 CDC 2936-7 CDC 3007-6 CDC 3007-6 /PI 660733 CDC 3422-8/PI 660729 CDC 3422-8 CDC 3422-8/PI 660736 CDC 3422-8 CDC 4053-4/PI 660736 CDC 4053-4 CDC Amarillo/PI 660729 CDC Amarillo
Parent 1 Parent 2 CDC 2847-21/90-2079 CDC 2847-21 CDC 2936-7/90-2079 CDC 2936-7 CDC 2950-19/90-2079 CDC 2950-19 CDC 3007-6/90-2079 CDC 3007-6 CDC 3422-8/90-2079 CDC 3422-8 CDC 4053-4/90-2079 CDC 4053-4
CDC Amarillo (yellow); CDC 2847-21 (CDC Inca – yellow); CDC 2950-19 (yellow); CDC3007-6 (CDC Spruce – green); CDC 3422-8 (green); CDC 3422-8 (green); CDC 4053-4 (green)
QTL Ae-Ps7.6 QTLs Ae-
Ps7.6 + Ae-Ps4.5
QTL Ae-Ps4.5
BC1F2 seeding
Apr 2017
Mar 2017
Parent1 x Parent2
Back crossing
Jul 2017
May 2017
F1 & back-
crossing
BC2F1 seeds
Aug 2017
Aug 2017
BC1F1 seeds,
BC2 crossing
BC2F2 seeds
Sep 2017
Jan 2018
BC2F2 seeds
MAS Dec 2017
Feb 2018 MAS
BC2F3 May 2018
May 2018 BC2F3
Pheno-typing
Jul 2018
Jul 2018
Pheno-typing
MAS Aug 2018
Aug 2018 MAS
Pyramiding Nov 2018 Pyramid
ing MAS Dec 2018 MAS Seed
increase Spring 2019 Seed increase
Seed increase
Summer 2019
Seed increase
Yield testing
Summer 2020
Yield testing
IDENTIFYING LENTIL WITH BETTER APHANOMYCES RESISTANCE
Lavaud et al 2015. Theor Appl Genet 128(11),2273-88
Gene pools in lentil
• Closely related species (primary gene pool) can be easily crossed
• Crossing with distantly related species difficult
Aphanomyces resistance in wild lentil germplasm
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Aphanomyces resistance in wild lentil germplasm
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Pathotypes of Aphanomyces
• French have identified 11 “virulence” types (pathotypes) • Screened for resistance with pathotype I (most common in
France) and pathotype III (Ae109; most common in N-America?)
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BaccaraMN313CDC MeadowPI660736
Canadian USA
A successful 2018!
Agri-Food Canada Agriculture and
CROP DEVELOPMENT CENTRE