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DNA Repair Pathways

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Copyright © 2012 Abcam, All Rights Reserved. The Abcam logo is a registered trademark.All information / detail is correct at time of going to print.

Polε

Polε

Polε

Msh2 Msh6 (MutSα)

Msh2 Msh6 (MutSα)

MutLα endonucleaseactivity andExo1 remove mismatch by 5’ to 3’ resection

RFC/PCNA andendonucleaseactivity of MutLαallow Exo1 to resect DNA 3’ of themismatch

Single base pair mismatchon lagging strand

Single base pair mismatchon leading strand

≥ 1 base pair insertion or deletionSingle base pair mismatch

Replication Resumes

PCNA

Mlh1 Pms2 (MutLα)

and

Msh2 Msh3 (MutSβ)

Exo1

Exo1

PCNA

Polδ/ε

Polε

5’

3’

3’

5’

3’5’

DNA Pol

5’

DNA Pol

DNA Pol3’

5’

PolδPolδδPolα

P

Polα

PolδPolδδPolα

PolδPolδδPolα

PolδPolδδPolα

RPA

XPG

UVDNA crosslinkers

Transcription coupled repairGlobal genome repair

Transcriptionblock PARP1

RPA

and

PCNA

XPE

HR23BXPC

HR23BXPC

HR23BXPC

TFIID

TFB5XPBXPD

TFIID

TFB5

RPPAA

XPBXPD

Polδ/εRFC

DNALigase

XPG

XPA

ERCC1XPF

ERCC1

XPF

RPA XPA

CSA

CSB

RNA Pol I/II

BRCA1 P P

P P P

Mre11Rad50Nbs1

Rad51BCDXRCC2/3

P

P

P

P

P

P

P

P

P

P

P

P

P

P P

P

P

BRCA2

BRCC36

BRCA2

Bard1

Ubc13

BRCA1

ATM

Rad52

Rad52

Rad51BCDXRCC2/3

BRCA2

P

BRCA1Rad52

ATM

Ionizing radiationGenotoxic chemicalsFree radicalsMechanical stress

Homology searchand D-loop formation

Homologous chromosome

DNA synthesis

Rad51 monomers

histones

γ-H2AX

PPγ-H2AXH2A

Poly ubiquitinon H2A

3’

3’

3’

BR

3’

3’

5’

5’

5’

5’

5’ 5’

5’5’

3’

Resection

Resection

SMC1

Mre11Rad50Nbs1

ATM

MRNcomplex

Hollidayjunction

resolvase

ATM

CtIP

CtIP

Rad54

Rad54

Rad51BCDXRCC2/3

ATM

Mdc1

DNAPol

DNAPol

Ligation and formationof Holliday Junction

Non-crossover(cut both junctionsvertically or horizontally)

Crossover(cut both black orboth red arrows)

DNALigase1

Rap80

Abraxas P

Rnf8

ATM

Caesin Kinase2

MRNcomplex

Nucleotide Excision Repair

P

P

P

P

P

P

P P

ATM

Polβ

Mre11Rad50Nbs1

XRCC1

Artemis

DNALigase 3

Ionizing radiationGenotoxic chemicalsFree radicalsMechanical stress

Homologousrecombinations

Strand openingand processingby exonucleaseactivity of WRN

No deletionAccurate repair

DNA ligation

Hairpin DNA cutby Artemis

Flap excision

Microhomologymediated strandannealing

Ku / Lig4 independentpathway

Strand tethering

Hairpin DNAends

Ku / Lig4 dependentpathway

Histones

PP P

Histones

γ-H2AX

H4K20Me2

XLF

PNKP

Mre11Rad50Nbs1

Ku70

Ku80

Ku70

Ku80

P

P

DNA PKcs

DNA PKcs

Ku70

Ku80

Ku70

Ku80

P

P

DNA PKcs

DNA PKcs

Ku70

Ku80

Ku70

Ku80

DNALigase 4

WRN

Fen1

WRN

Short deletionInaccurate repair

PARP1

PARP1

XRCC4

Rag 1/2

V JD

V JD

ATM

ATM ATM

53BP1

Nonhomologous End-Joining

Homologous Recombination Mismatch Repair

Produced in collaboration with James Haber and Farokh Dotiwala, Brandeis University.

UbqUbq

Ubq

Ubq

P

qPP

P P P P P

P

P

P P P P P

DNA crosslinkers, CisplatinMitomycin C, Fork stalling agents

Stalledreplicationfork

FANCD2/I

γ-H2AX

Repaired by NER

P

Histones

Repaired by HR

Translesion bypassby Rev1/3

Translocation tofork stall site

3’

3’

3’

5’

5’

5’

5’

REV1/3

3’

3’

3’

5’

5’

5’

5’

FANCB

FA100

FANCL

FANCM

FANCGFANCA

FAAP24

FANCEFANCF

FANCC

FANCB

FA100

FANCL

FANCM

FANCGFANCA

FAAP24

FANCEFANCF

FANCC

UBE2T

Chk1

ATR

FANCIFANCD2

ATR

E22TT

E1 Ligase

REV1/3

ATRIP

ATRIP

Polδ/ε

Polδ/ε

Polδ/ε

DNA Strand Crosslink Repair

Featured DNA Repair antibodies

Anti-BrdU - Proliferation Marker antibody (ab8152)

Hela cells stained with ab8152 at 1/100dilution.

Trial-sized aliquots of your favorite antibodies

Optimize before you commit.• DNA damage response• Histone modifications

• RNA polymerase II

For more details visit: www.abcam.com/panels

gamma H2A.X(phospho S139) antibody (ab2893)

Pachytenespermatocytes stainedwith ab2893 at 1/200.

DAPI

BrdU

Merge

043_12_KM DNA Repair Poster:Layout 1 27/02/2012 07:26 Page 1