Post on 08-Jul-2018
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DRUG SCREENING AND CLONING FACILITY
A) BSL II CELL CULTURE FACILITY:
A.1 MODULAR LABORATORY SET UP FOR CELL CULTURE
PARTITIONS:
Supply, Installation, testing and commissioning of self standing pre- fabricated insulated sandwich panels as per layout attached with 0.6 mm cold rolled PPGI Sheet on both sides with Infill material as PUF with all necessary accessories. Ceiling panels – 72 Sq.m. (30 mm Thick )
Supply, Installation, testing and commissioning of self standing pre- fabricated insulated sandwich panels as per layout attached with 0.6 mm cold rolled PPGI Sheet on both sides with Infill material as PUF with all necessary accessories. Internal Return Air Risers in 80 mm wall panel with Necessary Fittings – 52 Sq.m
View Panels 900mm x 900mm: - Double Glaze view panels for modular wall panels with 5 mm Toughened glass with all accessories like double adhesive tape, silicon sealant and silica gel with installation. – 3 Nos
Doors 900mm L x 2100mm H: - Supply of Door Panel in 50mm thick Overall section of 0.8 mm thick. GIPC/GIPC sheet on inner & outer skin infill with PUF of 40Kg/m3 density. With all Accessories( 'D'Handel , Door closer , Lock & key, kick plate , view Glass & Drop Seal ) – 6 Nos .
ELECTRICAL WORKS:-
RYB wiring with conduits. Switch Sockets – Material: Polycarbonate, 6/16A Combi Switch/Socket Clear uncluttered
modular design with more space for wiring. Distribution Board 3 phase wiring. IT & Tele Points ( Cat 6 , RJ 45)
FLOORING (CHEMOXY: 360 SQ.FT)
360 Sq.ft Area (Approx) With Peripherial Coving . The offered flooring system features
optimum chemical resistance and remarkable toughness which is best suited for industrial and commercial floors. Designed to withstand high traffic conditions the offered coatings are developed from premium grade materials and can be accessed with varied pigments.
Features: Remarkable glossy finish Self levelling High compressive strength Coving 40mm- 50mm in height filleted to avoid corner dust deposits
(i) Sectional Bench – 1 Nos :-
High end Stainless Steel Sectional Bench made up of Stainless Steel 304/316 of Size: L (As Per Site ) x350x610 mm with small rollers attached with the legs for easy movement.
(ii) Pass Box- 1 Nos
High grade stainless steel construction Mechanical interlocked Wall mounted design Chemical resistance CE and ISO certified UV light along with time controller Indicating lamp to know the door lock-unlock condition Suitable gauge for measuring pressure drop Hepa filter to maintains the required classification.
(iii) Classified Clean area (ADS) :-
For Class 10000 Area – 360 Sq.ft . Air Generator 2800 Cfm . Double skin in accordance with BS EN 1886: 1998 with 43 mm thick Puf insulation having
density 38 Kg/ cub mtr in construction, inner & Pre-coated outer skin of GI having 0.63 mm thickness. AHU with thermal break profile, coving.
Pre filter section with 10 micron filter. DX coil section with 6 row deep with SS drain pan Fine filter with 3 micron. 90% re-circulating Air & 10 % Fresh Air Height of false Ceiling 8 ft from Ground Level. Chilling Units:- (5.5 + 5.5) Tons. :- Having Hand Valve, Dryer, Expansion valve & Nitrogen
Pressure testing. Vacumising, Gas charging & commissioning of same unit. Humidity: - 55-60 rh. Temperature 24±1 . Positive Pressure.
THERMAL INSULATION (1800 SQ.FT) :-
24 gauge Factory fabricated Ducting made out of GI sheet with proper gasketing, supports, rods etc and fabricated as per standards. With MS angle flanges & RTV sealant. Galvanised Iron Ducting.
Insulated with 16mm thick class O type Insulation. Canvas Connections to return air riser Dampers. Framed angle joints with leak proof sealant. Copper Piping with insulation.
FRESH AIR SUPPLY DAMPER WITH HEPA FILTERS (07 NOS). :-
12 Guage GI Powder Coated Terminal Box of Desired CFM Side Connection Square Gear Operated VCD and S.S Perforated Grill. 3 Micron pre filter for
Supply Air. Bottom Operated Damper. Return air risers:- S.S Perforated Grill. 3 Micron pre filter for Supply Air. Bottom Operated
Damper.
VALIDATION:-
Dry Run, Air Balancing, Particle testing Velocity Test & Pressure balance which helps to maintain the desired class of the clean room
LAB FURNITURE:
Wall bench of approximately 600 mm depth Required with Jet Black Granite Top 18 MM THICK with Zero water absorption, high temperature, chemical and stain resistance and can be re-polished. Drawer units are required below the wall bench and should be removable.
Drawer units:
1) Drawer Unit (600 mm wide 2 Shutter 1 Drawer End Unit) 600 mm wide, 550 mm deep. - 28 Nos
2) Drawer Unit (450 mm wide 1 Shutter 1 Drawer End Unit) 600 mm wide, 550 mm deep. - 7 Nos
3) 750 mm height lab storage unit having drawer of 125 mm in height with two telescopic channels
4) Below drawer one/two shutters are mounted with SS hinges. Unit is having one shelf in side.
5) Unit is also having 100mm toe space at bottom, complete unique.
6) Complete CRCA furniture is pre-treated with 8 tank pickling process further chemoxy powder coated by maintaining 70 to 80 micron film thickness.
POWER TRUNKS (18Mtrs) :-
1) POWER TRUNKS (1200 mm in length)1200 mm wide,120 mm deep, 120 mm Height table mounted Raceways.
2) Electrical Switch & Sockets Fitted (With Wiring).
3) Complete CRCA furniture is pre-treated with 8 tank pickling process further chemoxy powder coated by maintaining 70 to 80 micron film thickness. Switch & sockets are fixed as recommended from client.
OTHER REQUIREMENTS:
(i) AIR SHOWER SS304
Adjustable time (10-99sec) with HEPA filter and washable pre-filter with 90% arrestance of dust air to extend the life of the main HEPA filter.
The Air shower system should be auto controlled interlocking systems, the entry and exit door is electronically interlocked, and Photoelectric sensor suitable.
The Air shower system design should be modular type for easily transportation and assembly
(ii) APRON STORAGES (1 Nos) : 12 Garment storage capacity Apron Storage with UV light & sterilization of approximate size of
1000 mm L x 320 mm D x 1800mm H
(iii) DOCUMENT STORAGE UNITS - 1Nos
1) Document Storage Unit Laboratory Chemical storage unit size 1000wx 450dx1800h.
2) Made of 24 gauges C.R.C.A. Mild Steel.
3) 4 Nos adjustable shelves inside.
4) Swing doors perforated down and hinged on SS hinges which is not exposing inside.
5) Inside walls are covered with chemical resistant laminate/pp sheet (Optional) .
6) Complete unit is pre-treated with 8 tank pickling process further epoxy powder coated by maintaining 70 to 80 micron film thickness.
(iv) COLD ROOM FOR PROCESSING -1 Nos (6ftx8ft)
Temperature range: 4 to 15 with digital temperature display and chart recorder. Electrical fixer, table with granite top 3ft x 6ft.
(v) PREPARATIVE MINOR EQUIPMENTS:
Vortex – 2 Nos Table top spin centrifuge (12-15k rpm; 8-12 position) Pipette set for cell culture preparation (Single channel 1-5ml, Pipetteman and Multichannel
30-300μl) Magic Mixer & Magnetic stirrer Water Bath with Digital Display Domestic refrigerator 300 to 400 L capacity Liquid Nitrogen Container: Capacity : 31.5 Lit. Static evaporation loss rate : 0.11 lit/day
General Layout of Modular Lab
A 1.1 BIO SAFETY CABINET 2 NOS FOR PROCESSING OF CELL LINES
Specifications:
Bio Safety Cabinet Class II Type A2, Size: L X D X H : 3’x2’x2’
Applicable Standards -EN ISO 14971:2012,EN ISO 13485 : 2012 , EN 980:2008 , EN 1041 :
2008 , EN 61010-1:2010, EN 61326-1:2013, EN 12469:2000
System should Provides the Product, Person and Environmental protection Velocity of airflow to the work zone creates a class 100 environment for product protection,
where the remaining exhaust air is discharged out through (HEPA). Air barrier at the work opening & the contaminated air plenum under positive pressure. An air barrier between the operator & the work zone maintained by a flow of room air through a
full width grille in the work opening. The barrier air mixes with the laminar flow air in a sump underneath the work surface & is
exhausted to the outside environment through a HEPA filter. Should be ergonomically designed to improves comfort, preventing user fatigue & to promote
safe working.
Main body – Mild Steel Powder Coated
Work Surface –Seamless ,Scratch-free, high quality 18 gauge stainless steel, grade 304
Working tray – Autoclavable & Removable stainless steel work surface for easy cleaning.
Air Flow: 70% recirculation through HEPA filter & 30% exhaust through HEPA filter
Filter Type – HN 14HEPA filter with integral metal guards & frame gaskets. Supply of HEPA
filter 99.997% at 0.3 micron.
Front Control Panel –should be provided to avoid exposure of UV Light & Lamp outside,
making eyes more comfortable.
Front Sash Door - Manual sliding sash door preferably made up of poly carbonate sheet or
toughened glass with sloping front for better visibility of workspace &
access of samples. Front sash door height should be adjustable as per
requirement by end-user.
Air Velocity - 100 ft/min ± 10
Air Volume - Up to 500 CFM
LCD Display – Should be digital microprocessor control System for Operating Fluorescent, UV Light & Blower. Sould continuously display true value of differential pressure of HEPA filter for inflow as well as down flow velocity. The location of display
should be on outside of the Biosafety Cabinet for easy use & also easy to reach from a seated working position.
Sleep Mode Operation – Automatically blower speed reduced up to 30%, this will help to save
energy as well as help to maintain sterile work area during Biosafety Cabinet is not in operation
Intelligence Alarm System – Safety purpose Audio & Visual alarm for air fluctuation and for
life of the HEPA filter and UV light Ultra violet tube light – For Germicidal activity i.e. 254 nm Ultra violet life meter- Continuously display UV Hour on display UV Light programming - Available with timer & UV Hour meter to avoid operator risk Interlocking UV - The closing/opening of front door is integrated with the UV ON/OFF.
The UV will automatically switch “OFF” when the sash door is opened & switch “ON” when door is closed to avoid accidental exposure of UV light to the operator.
Electrical Socket - Internal socket inside the chamber, 5/15 Amp Illumination of Work surface- Fluorescent tube light (intensity > 600 lux) provides excellent
illumination for work surface & reduces operator fatigue. Fluorescent tube light in set behind front control panel.
Working Noise level - should be < 65 dB Electronic / Electrical panel- From clean chamber to give better contamination free results. Electrical Safety – Should have standard quality for better electrical safety for the operator Heat Emission - Ambient BTU/hr (kW) at 25°C Power supply - 230 V ± 15%, 50 Hz ± 3% (Compatible to Indian Type) Arm Rest Bar - To avoid contamination from outside to inside contamination & for easy
working with comfort. Secure & comfortable armrests enhance your comfort during extended work sessions
Drainage Port- Preferable which may provide beneath work surface to facilitate easy & better
cleaning of the interior & handling of spillage inside the chamber
Preferred Performance Certificates –
ISO 9001 : 2015 CE Marked : CE Certificate GMP Certificate, EN ISO 14971:2012 Applicable
Standards, IQ,OQ& PQ. Calibration & Traceability certificates, Factory tested DOP certificate.
A 1.2 CO2 INCUBATOR –(2 NOS)
Specifications: Direct Heat CO2 Incubator. It should have chamber volume of 160 litres minimum. Chamber dimensions : 20.25 x 20.678 x 24 inches Inner chamber is constructed of, 100% stainless steel, to reduce chance of corrosion or
contamination. It should have color touch screen LCD panel, displaying accurate level of CO2 & temperature,
alarms conditions and built-in data logging facility. It should have high temperature sterilization facility with choice of Dual sterilization cycles :
145OC or above (Dry) and 95OC (humidified) Temperature range : ambient +3 to 55 OC Temperature Uniformity : + 0.3OC Temperature Accuracy : + 0.1OC Temperature recovery : 0.12 OC CO2 sensor should be TC/IR single source dual wavelength. Preferably Sensor should be in place
during sterilization CO2 control range : 0.1 to 20% CO2 Accuracy : + 0.1% Should have fastest recovery of CO2, 5% within 5 minutes Should include 4 shelves with max capacity of 16 or more. It should have removable humidity pan. Incubator should be stackable model. Should have closed loop HEPA filtration system Filters should locate outside the chamber with easy access to replacement. Interiors should have curved corners for easy cleaning. Should have remote alarm contacts, 4 to 20mA Analog output, RS-485 communication, USB
port Should have access port and plug with breather holes. Power : startup 625 Watts, running 250 Watts, Heat rejected: 14 BTU/min. Electrical : 230VAC, 50Hz (Indian type)
A 1.3 REFRIGERATED CENTRIFUGE (2 Nos)
Specifications:
Maximum Speed of 30,130xg/17500rpm, with a brush less motor.
Temperature range should be from -4°C to 40°C, should be able to maintain 4°C at maximum speed.
It should be possible to use aerosol tight rotor, aerosol tightness of rotor should be certified by a third party agency.
Instrument should come with different types of rotors like test tubes, falcon tubes (15ml and 50ml), micro-centrifuge tubes (0.2ml to 2ml), 96 well plate and large container rotor.
Instrument should have an in-built condensate drain to prevent water accumulation.
It should be possible to perform a fast pre-cooling of the instrument.
It should be possible to program fast cooling with preset date and time.
It should be possible to store many programs with quick access program keys.
Speed setting should be possible in both rpm and rcf.
Should possess a separate short spin key for brief spin.
It should be possible to program compressor shut off after 8hrs of non usage of the centrifuge
Instrument should have automatic rotor recognition facility to automatically recognize and set maximum speeds upon rotor change. Hassel free Rotor removal is preferred.
Rotor lids should have a quick lock system for secure lid closing and opening
It should be possible to perform gentle acceleration and deceleration using dedicated key
Noise levels should be � 60 db (A)
It should be possible to operate the centrifuge at set rpm, for short spin protocols
Instrument should be CE Certified.
A 1.4 FREEZER (-20 ) – 1No
Specifications:
Gross Volume 380 liters
Vertical Solid door freezers
Dynamic Cooling ( Frost Free)
Temperature range between - 17 to – 24
Environment friendly refrigerant and CFC free refrigerants R-134A
Single solid Door outside and inner doors for each shelves
Compressor : International standard
Controller : International standard
Optimal energy saving machines due to better condensing capacity.
Tropicalized i.e suited to take on harsh Indian temperature conditions,
It should perform even at 45 -17 to - 24 and Easy to adjust temperature.
Uniform Digital temperature display
Foaming door with dual insulation for better refrigeration effect
Quick freezing Facilities
Dimension: 600 x 600 x 1870 mm
A 1.5 ULTRA LOW TEMPERATURE -86 DEEP FREEZER (1 No) Specification
Upright ultra-low freezer with Internal Dimension of H x W x D =120 to 130 cm x 53 to 57 x 50 to 60 cm approx. and External dimension of H x W x D =190 to 195 x 80 to 85 x 83 to 90 cm approx.
Freezer should be of minimum capacity of 410 Liters. System should have Programmable operating temperature from –50°C up to –86°C with 1°C
increment It should be an energy efficient system with power consumption ≤ (less than or equal to) 11 kWh
per day under standard test conditions* System must be fully functional in operating temperature range (ambient Temperature) from
10°C upto 32°C System should have a pull down time from ambient temperature to -85°C ≤(less than or equal to)
5.5hrs Fully programmable microprocessor controlled with membrane keypad and eye level control
panel. Construction should be of Polyurethane foam (130mm thick) insulation. System should be made up of 18 gauge Steel, 1.2 mm thick with powder coated paint to resist
scratch and rust and the interior should be Polished with 304 SS for easy cleaning and to eliminates potential for oxidation.
Heated air vent with ice-cleaning plunger prevents vacuum formation, allowing the door to be quickly & effortlessly opened anytime in the day
Inner door should have silicone seal to prevent temperature loss and Outer door should have safe silicone triple point seal with flat soft sealing on outer door which is easy to clean, durable and prevent ice formation. It should be no folded gaskets which leads to ice formation in between the folds hence making the gasket seal less durable and requiring replacement often
Freezer should have 5 Compartment with five inner doors. Freezer should have the sample (2” / 5 cm vials) capacity of 24,000 or more. Freezers should have heated air vent and front panel air filter. Should have security keyed locks on the outer doors and lids keep out unauthorized users Freezer must have battery back-up and 4 PIN security lock for unauthorized tampering. Freezer must have RS 485 interface data logging port. Audible and visible alarms for temperature, power failure, system failure, battery low etc. and it
also have remote alarm port for connection to an auto dialler. Freezer must use CFC-FREE, HCFC-FREE non flammable refrigerants, and refrigeration system
must be energy efficient and hermetically sealed two stage cascade refrigeration system. Compressor should be capable to run any voltage between 190 – 270V. Freezer must have ISO 9001 standard quality test requirements and IEC 61010 Electrical safety
CE & UL certified. Freezer should have efficient power consumption in the range of 10 to 11 kWh/day Freezer must have capacity to hold 15 racks and 240 boxes of 2”/ 5 cms height vials (Total
sample (2” / 5 cms vials) capacity of 24,000 or more) Freezer should have electric supply of 230v/50hz, 10 amps. Single phase Freezer should be
supplied with 5KVA voltage stabilizer. Back Up for CO2 required. data logging software required
A 2. TISSUE DISSOCIATOR (For making single cells from tumour and normal tissues)
1. Equipment should be capable of simultaneous processing of 2 samples at a time 2. It should dissociate tissues into viable single-cell suspensions and/or homogenization 3. It should have specific optimized programs for processing of various tissue types. 4. The system should allow safe and sterile sample handling with individual disposable/autoclavable tubes. 5. Tubes should be equipped with a pierceable septum for safe extraction of dissociated material. 6. Power: 220-240V (Indian standards) 7. Rotational speed 200-4000 rpm with overload restriction and rotation clockwise or anticlockwise 8. Operational temperature: +4 °C to +35 °C & Humidity 15-80% (Noncondensing) 9. USB interface desirable
A.3. INVERTED CELL MONITORING SYSTEM (FOR REMOTELY MONITORING CELLS
IN REALTIME)
Specifications:
System should be user friendly and operate easy handling
Compact in size – must be fits in every incubators
Cloud technology – Monitor the cell culture anywhere and anytime
The affordable live cell imaging tool for small budgets
Cloud-dependent functions such as alerts or confluence determination
Available via Wifi and LAN connection
Taking snapshots in offline mode with local storage of images
New optical system with improved resolution
Zoom functionality provides images and videos in 2-fold zoom-in
Migration assays, e.g. scratch assays – Automatic photo/video documentation
Stem cell culture documentation – Remote monitoring under hypoxic cell culture and GMP
conditions or of iPSC reprogramming
Cell culture standardization – Automatic alerts for confluency, recording of cell growth curves
and screening of culture media and growth factors
Power Supply : AC 240V, 2A, 10W, 50/60Hz
Field of View : 2.4 x 1.4 mm
Magnification : 10X
Image Resolution : 1280 x 720 pixels (converted to 640 x 360 on webpage)
Exported Formats : JPEG, CSV, AVI
Light Source : LED
Camera : 5 MP CMOS
Display Remote : 10” tablet touchscreen
Operating Environment : 5-40oC, 20-95% humidity (suitable to be placed inside incubator)
A.4 SINGLE LASER CELL ANALYSER
Specifications
System should be able to analyse following sample types:
• Homogeneous or heterogeneous, • Suspension or adherent cells. • Primary cells or cell lines (Mammalian & insect cell line)
Input Cell Numbers : >10,000/ml
Optics – Laser : Green – 532nM diode laser
Optics –Detectors: Optical module with three detection channels 3 Photodiodes for detection of:
• 2 fluorescent colors: >Yellow fluorescence detection at 575nm +/-12.5nm >Red fluorescence detection at 690nm +/-15nm
• 1 scattering signal
Fluidics: Microcapillary based standard square flow cell
Pump: stepper motor syringe pump
A.5 SINGLE CELL ISOLATOR Specification
Application:- Manual isolation of cells from a unique array on a petri-dish and transfer to secondary vessel.
System Should comprises of a controller, power supply for controller, a magnetic collection
plate, a motorized release device, 4 inserts for release device, protective lid for release device and a magnetic wand
Consumable:- array with dimensions of 0.2 mm x 0.2 mm for each microraft
Hardware:- Altitude:- Up to 2000 m (6500ft), Controller; Input voltage: 12 VDC; Power: 6
W; Width: 94 mm (3.7 in); Depth: 132 mm (5.2 in.); Height: 46 mm (1.8 in.); Weight: 0.3
kg (0.66 lb)
Environmental Class:- 3K2 (IEC 60721-3-3); 3M2 (IEC 60721-3-3)
Specifications of the System should be like Device temperature: 30°C ± 10°C; Motor
connection: Snap and lock connector; Insertion force: 4.5 kgs max.; Withdrawer force: 0.5–3.0 kg; Motor actuator speed: 400 steps/sec; Dislodging action: 1 needle with moving platform
Power Supply:- AC Power Adapter;
Width: 39 mm (1.5 in); Depth: 76 mm (3.0 in.); Height: 56 mm (2.2 in.); Weight: 140 g
(0.31 lb); Input Voltage: 100–240 VAC; Mains supply voltage fluctuations are not to
exceed ±10% of the nominal supply voltages; Input Current: 0.45–0.2 A;
Frequency: 50–60 Hz
A.6 Inverted Fluorescence Microscope
Microscope Stand:
• Ergonomically designed stand for Tissue culture studies • Rugged and sturdy Inverted stand. It should have low positioned co-axial coarse and
fine focus knobs • Suitable for Bright field and Phase contrast with DIC compatible to plastic surface.
Scope of future upgradation to DIC/Improved Hoffman Modulation Contrast for Fluorescence Studies
• Built in Electronic Power supply for 12V/37W or 100W illumination with conveniently positioned ON/OFF switch
• It should having Provision for attaching the Long life LED illumination, also should be
possible 45⁰ Binocular Phototube with Interpupillary adjustment from 48-75mm and
'Sidentopf' desiged tube at two different viewing height at 360 and 397mm • 13mm focus lift with adjustable focus stop • Special skin-friendly material coated on all operating elements
Modular Illumination: 12V 37W halogen illumination as well as LED Illumination
Eyepiece: 10x with a field of view number of 23 with diopter setting +/- 5 on both eyepieces. The eyepiece should be suitable for graticule insertion
Nosepiece: Focusable Quintuple (5x) or Sextuple(6x) Nosepiece having revolving nosepiece with highly precise click stops
Objectives:
Infinity corrected objective Normal and Long Working distance objectives of 5x, 10x Phase, 20x Phase and 40x Phase Anti-fungal treated Optics
Condenser:
• Long Working Distance Condenser 0.3 with a working distance of 72mm with inserts for bright field, Phase contrast and DIC(for plastic surface)
• Should be suitable for the magnification range from 50x to 400x • Universal Phase Slider for all the objectives • The condenser should be moved backwards, so that the large flasks can be
accommodated on the mechanical stage
Stage:
• Two layered stage • Specimen Stage 232x230mm • Objective Traverse 130x85mm • Universal Mounting frame
Fluorescence Attachment:
• Fluorescence light attachment with 100W/120W HBO mercury illumination source for fluorescence excitation should be offered
• 4 position reflector turret to house upto 3-4 fluorescence filters • Filters should be easily insertable and removable in the reflector by push and click
method in a module • Filters for DAPI, FITC and Rhodomine to be quoted
Head: Camera Adapter:
Trinocular head for mounting Camera Camera Adapter 0.63x or better
Camera Attachment:
3 Mega Pixel cooled CCD camera or better with a Basic resolution of 1936 (H) x 1460 (V) = 2.83 Megapixel, Pixel size 4.54µm x 4.54 µm, Chip size of 8.8 mm x 6.6 mm. Connection cable with driver software 64 bit, USB 3.0 connection cable with 3.0 m with a suitable software. Max Full well capacity: 15000 e
Microscope Camera and Software should be from same manufacturer for better compatibility and should support the micromanipulator. A.7 Micromanipulator System for manual injection into Animal cells and/or Embryos. Recommended Configuration:
• Electronic stepper motor based microinjection system (Injection arm and holding arm should be quoted separately as an optional)
• Hydraulic Injector – 01 Nos. • Pneumatic Injector – 01 Nos. • TransferTips (ES) • VacuTips • Microscope Adapters [either Microscope specific in which case 01 Nos. OR Universal adapters
in which case 02 Nos.] System Description: • System consists of single Manipulators and single manual injectors, one manipulator for moving and positioning the injection needles for precisely manipulating, picking up cells and/or solutions (eg., DNA)for the injection cells into the egg cells / embryos (oocytes) by a hydraulic manual Injector which supplies the intended pressure. • The system should be flexible enough to be installed, adapted & used on left or right side of all commonly used microscopes (e.g., inverted microscopes, upright microscopes and stereo microscopes). Manipulator features: • The system should be a fully electronic system. All X, Y, Z axis movements are controlled electronically. • All three axis movements at all speed control should be controlled with one single integrated joystick. The system should have the coarse, fine, extra-fine movement selections using a single upright joystick. • The system should have resolution of not more than 0.02 μm (20 nm) increment for fine and smooth movement. • The system should have a Dual Speed joystick: proportional/direct movement and dynamic movement. With the proportional movement, the movement of the joystick is directly transmitted to the movement of the capillary. With the dynamic movement, the speed of the capillary is increased by the degree of joystick deflection. • The system should have a tool travel speed of at least 10,000 μm/s, for rapid work and penetration of tough material. • The system should have option to set both upper & lower programmable Z axis limits • The system should have an automated function for the capillary to move out of working radius and return to the precise same spot, called “home” and “Clean” functions. • The manipulator should not have handedness and it should be possible to mount it on either side of any Microscope Injector Features: • The injection Micro-injector should be Maintenance-free piston/cylinder systems • System should have Large, ergonomic dials for control • System should have Stable, non-slip base plate • System should be supplied with Universal capillary holder for mounting & holding capillary needles/tips onto all common micromanipulators Features of the Hydraulic Manual Injectors • The injection system should be a manual hydraulic system with Variable transmission ratio (1:1, Coarse control and 1:10, Fine Control) for applications that demand high resolution and sensitivity
• It should have a Quick Valve system for simple, rapid oil filling • It should be capable of generating a Maximum pressure of upto 20,000 hPa • Biological compatibility of the system should be ensured by embryo-tested oil (Sigma M-8410)
B) CLONING FACILITY
B.1 PORTABLE TUBE LUMINOMETER
Specifications:
Purpose: for all common applications using to visualized bioluminescent and chemiluminescent reagents.
Detection unit : Photo-multiplier with single photon counting mode
Spectral range : Around 380nm - 630 nm
Dynamic range : 6 orders of magnitude
Sensitivity : <100 amol ATP &<50 zmol luciferase Tube format : It should be compatible to standard micro-centrifuge tubes(2ml,
1.5ml, capped and open vials)
Display : In-built LED display or with PC connection
Software : It should have recommended in built software
Data storage : More than 1000 events
Certification : CE certified
Operating Language : English
Power Supply : 230 V (Indian type compatible)
PC connectivity : Should have with PC and PC interface
B.2 BIOREACTOR
Specifications
1. The system should be a compact bench top bioreactor capable of batch, fed-batch , continuous
and perfusion processes. It should be possible to use the same system for both fermentation as well as Mammalian cell culture.
2. System should be scalable from 250 mL to 40 L on a wide variety of autoclavable and Single-Use Vessels.
3. System should support 5 L working volume with cell culture magnetic drive vessel.
4. System should be compatible with twenty four(24) interchangeable autoclavable vessels of heat blanketed and water jacketed vessel types as well as Single use solid-rigid wall stirred tank vessels including cell culture, packed bed, and fermentation 1L to 50L variants.
5. Vessel should have hemispherical vessel nest design for minimum footprint. Controller should be with a minimal footprint, about 10 in (25 cm) wide or less.
6. The autoclavable glass vessels should have about 10 headplate ports.
7. System should have built in Process modes offer process control for microbial and cell culture applications at the touch of a button.
8. The system should be provided with two flexible universal connections for analog or digital Mettler Toledo sensors of ISM type (Intelligent Sensor Management)
9. System should be supplied with pH sensor and Optical DO sensor. • pH control range should be from 2 – 12. • DO control range should be from 0 – 200%.
10. It should be possible to control the temperature from 5°C above coolant temperature to 65°C
above ambient (0°C - 80°C maximum).
11. Redox sensor with a control range from -2000mV to + 2000mV should be available as an option.
12. CO2 sensor with a control range of 0 – 100% should be available as an option.
13. System should be equipped to view the entire process on system controller screen with expanded trend screen for up to 8 values /parameters ,
14. The system should be provided with connections for interchangeable direct- and magnetic-drive motors; magnetic drive capable of clockwise and counterclockwise rotation for simplified impeller selection .The motor speed should be in the range of 25 – 1200 rpm for direct drive.
15. System should have two flexible universal connections for analog or digital Mettler Toledo sensors of ISM type (Intelligent Sensor Management)
16. The system should be supplied with 1 Mass Flow Controller (0.4 – 20 SLPM) with 4 gas mix control and sparger. Automatic gas mixing algorithms for simplified control, and User-defined DO cascades should be there for process flexibility
17. System should be built in with three user-defined analog input/output connections to select between 0-5V, 0-10V, and 4-20 mA depending on the device.
18. The system should be supplied with minimum 2 USB ports; 3 Analog Input / Output modules and Ethernet connectivity for simultaneous control of up to 8 systems via SCADA software and IP network for remote monitoring.
19. High-precision thermal mass flow controller (TMFC) or rotameter for gas flow control. The system should be supplied with 1 Mass Flow Controller (0.4 – 20 SLPM) with 4 gas mix control and sparger with universal gas control strategy for both microbial and cell culture application, without the need for additional equipment.
20. Every system should be with it’s own Operators’ Interface Terminal ( OIT) with touch screen monitor.
21. System should come with IP21 rated enclosure for protection of sensitive electronics and cleanability.
22. System should be supplied with three front-mounted fixed-speed pumps with industry-standard easy Ioad pump heads for convenient liquid addition/ removal, could be used in fixed speed & % Pulse Width Mode ( PWM) mode. ( 30 RPM)
23. System should additionally have a provision to accommodate up to 3 more variable speed drive pumps externally.
24. Chiller, Compressor and other require accessories supply locally with instrument
B.3. TRANSFECTION SYSTEM FOR MAMMALIAN CELLS
Specifications
Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells
Classification: Animal Origin-Free, Chemically Defined, Low-Protein, Reduced Growth Factors, Reduced Serum, Xeno-Free
Current Limit: 3.0-6.0 A
Format: Electroporation (Tip/tube or cuvette)
Input Voltage: 220-240 VAC (Indian type)
Power Limit: 300 W
Reaction Time: 1 sec or less
Sample Loading Volume: upto 100µl or more
Sample Type (Specific): Plasmid DNA, Synthetic siRNA, Should be Serum Compatible
Starting Material (Cell #): Should be able to handle 2x10^4 - 6 x10^8 cells
Voltage Limit: 0.5-2.5 kV
B.4 TABLE TOP REFRIGERATED ORBITAL INCUBATOR SHAKER
Shaking specification:
Shaking Frequency Single unit : 30-350 RPM Double unit : 25-250 RPM Shaking Deviation : ± 2 RPM Shaking Display : Digital LED/ light screen Shaking Change via : Potentiometer Shaking Motion : Orbital Diameter of Orbital Motion : around 25 mm Platform Dimension (W x D) : approx 420 x 420 mm Movement of the shaker comprises Triple Eccentric Counter Balanced drive with 7 permanently
Lubricated Ball Bearings Drive : PMDC Universal Aluminum Platform which can accommodate standard clamps of sizes from 25 ml
upto 2 L • Approx 36 Nos. of 50 ml • Approx 36 Nos. of 100 ml • Approx 25 Nos. of 250 ml • Approx 16 Nos. of 500 ml • Approx 4 Nos. of 2000 ml
Temperature specification:
Temperature Range : 4 /10°C to 70°C/80 Temperature Accuracy : +/- 0.5° C Permissible Ambient Temperature : 5°C to 40°C Temperature Sensor : Pt100 Temperature Controller : PID Temperature Display : Digital LED/Light screen Soft Touch Keypad based control or manufacturer standard controller
Other specifications: Front/top open door with large viewing mirror Material of Construction : Interior and Exterior MS Powder coated Internal Dimensions (W x D x H) : Approx (490x 500x 412) mm External Dimensions (W x D x H) : Approx (525 x 730 x 800) mm Can be stacked upto 2 Levels using Stack Rack / Stacking Kit Alarm for temperature deviation. Thermostat cut-off for run away safety Automatic Power failure restart Unit has facility of proximity sensor to control the shaker on/off position Electrical Requirement : 230V,16 Amps, 50 Hz, 1Φ
B.5 LIQUID CHROMATOGRAPHY BASED PROTEIN PURIFICATION SYSTEM
Specifications:
Liquid Chromatography (LC)
• Versatile Chromatography System for protein purification that must be automated with a flow rate range from 0.001 to 10 ml/min or more
• Flow rate accuracy ± 2% to 3 %
• Pressure range: 0 to 25MPa or more (for compatible to connect HPLC columns as well)
• Viscosity range around 0.5–10 cP
Conductivity monitor must be integrated with following specifications:
• Conductivity reading which can be monitored in the range of 1 μS/cm–999 mS/cm • Accuracy ± 2%
UV Detector:
• Light source: LED based technology, which is durable, reliable, and ready to use at start-up and which does not heat the sample.
• Detector: Detector should be able to detect two wavelengths simultaneously within the UV-VIS range and more preferable at 260 and 280 for DNAs/RNAs and Proteins respectively.
Automated Sample Injection:
• System must be capable of carrying out accurate gradients equipped with automated sample injection.
Automated Fraction Collector:
• Automated fraction collector compatible for the system with option to collect fraction in 96 well plates/test tubes/micro-centrifuge tubes with suitable diverter valve and fittings kit.
• Fraction collector should be capable to use independently also.
Software:
• Single user licensed software and easy to operate • Analysing runs performed on various FPLC instruments from different vendors • Simple and easy operation for integration and overlay features • Column libraries of Pre-packed columns from different vendors/manufacturers
Other desirable Requirements:
• Easy to understand plumbing with visual assistance to minimize the training requirement of new researcher
• Guided Flow path Interface for graphical system flow path control from set up to operation • LED status display for system status at a glance • System must come with advance computer facility with large monitor display (29” or more)
B6. Specification for Protein fractionation unit for 2D separation
1) System should provide with preparative isoelectric focusing apparatus for fractionation of
protein and peptides on basis of pH
2) System should be capable of liquid phase recovery of the fractioned protein or peptides
3) System should Fractionate 2 x 8 samples (12 or 24 fractions) in OFFGEL mode and
IPG‐IEF (in gel) on 2 individual trays
4) System should be capable of maintaining Humidity < 80% at 40° C (104 °F)
5) System should have Ambient operating temperature 5 – 40 degree C
6) System should have Flash Memory storage capacity
7) System should support μg to mg load capacity
8) System should have simple user interface for online control of each individual sample
9) System should be Simple, reproducible, and easy‐to‐use
B7. Chip based Electrophoresis System for nucleic acids and proteins
1. The system should be microfluidic multichannel chip based electrophoresis for qualitative &
quantitative analysis of DNA, RNA and protein.
2. The system should offer fast automated analysis of >10 samples simultaneously with small sample
volume (<5 micro lt).
3. The system should have highest resolution and sensitivity (upto pg/µl concentration).
4. The System must have RNA quality check with RIN (RNA Integrity Number)
5. The system should have dedicated QC applications for NGS Workflow & Small RNA.
6. The instrument should be Easy to handle, user friendly software with capability of sample comparison
within and across the chips and minimum exposure to hazardous reagents
7. The system should have powerful software for the analysis of RNA, DNA and proteins
8. System should have various data-display options e.g. gel view, electropherograms and tables.