Exploring a Putative Gene

גיא וקסמןרועי נבון

Problem definition

Taking one of the thousands of Putative genes that were “found”

In the human genome project & Trying to determine whether this Sequence might be a real gene that canbe translated into a protein product.

The Selected GeneThe Selected Gene::

NM_014727

Homo sapiens myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4).

PSI-BLAST

Many entrances are actually on thesame locus (SNPs, flanking, different

Alleles etc .)`

The Locus problemThe Locus problem

Different Entrances on the Same Different Entrances on the Same Chromosomal RegionChromosomal Region

Conserved DomainsConserved Domains

SET1

CXXC zinc finger

Zinc finger

2-BLAST between the putative MLL4 gene & the most similar real Gene-Trithorax, from DrosophilaMelanogaster.

E=5e-87

E=2e-82

22--BLASTBLAST

Multiple AlignmentMultiple Alignment

SET1 domain

3D Structure – 3D Structure – Zinc Finger DomainZinc Finger Domain

Biological AspectsBiological AspectsDrosophila melanogaster

The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains.

Mazo AM, Huang DH, Mozer BA, Dawid IB

ככה נראיתי פעם...

Homo Sapians

Acute mixed-lineage leukemia t(4;11)(q21;q23) generates an MLL-AF4 fusion product

ConclusionsConclusions

1 .We found a high similarity between our putative gene and genes from other

species.

2 .The MLL4 putative gene contains at least 3

conserved domains .

3 .The homologue genes from Drosophila and

Human are associated with the nervous system and leukemia respectively .

Possible directions for Possible directions for future researchfuture research

1 .Trying to find DNA motifs which bind to our putative gene and to its homologues.

2 .Trying to isolate the full length protein .

3 .When 3D structure prediction will be available, we’ll try to compare our putative

protein with other known proteins.