Copyright Cosucra 1

Feed

your

world

dr Loek Pijls

for Cosucra

Copyright Cosucra 2

Copyright Cosucra

Nice to meet

3

https://www.youtube.com/watch?v=Wrxxv_8rL1I&list=PL-UqyRlowYJwfQCN5fMIy-VEanGbzeJYC

Copyright Cosucra 4

“Ask not (only) what your

microbes can do for you,

but (also) what you can do

for your microbes”

Copyright Cosucra 5

Feed

your

world

• We eat

• Left-overs small-intestine -> enter large-intestine

• Dinner for microbes in large intestine

• Their metabolites:

• change colonic “weather”

• impact colonic cells - barrier

• are absorbed into & impact whole body

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WHAT ARE WE LOOKING AT?

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What are wheat AXOS?

AXOS = Arabino-Xylo-Oligo-Saccharides

From wheat bran - naturally sourced

Chain of xylose, substituted by arabinose &

containing ferulic acid units: anti-ox, anti-

inflammatory

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Origin of Fibruline chicory inulin & oligofructose

Fibruline® (inulin) & Fibrulose® (oligofructose): soluble fibres, gently extracted from the root of chicory (Cichorium intybus L.).

16%

7%

3%

Water

Inulin/oligofructose

Pulp

Other soluble matter

74%

Our chicories are locally grown in Belgium and North of France

We supply our farmers with our chicory seeds (Chicoline), and ensure good agricultural

practices

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What is chicory inulin? What is chicory inulin? What is chicory inulin?

• Linear chain of fructose units

• Feed for chicory leaves, blossom, fruit &

seeds - like breast milk for a baby

• Most well-established prebiotic

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From Belgium

12

Inulin & oligofructose in Europe

Spelt & poppy seed yogurt Danone, Austria 2016 Contains oligofructose

Source: Mintel -GNPD Wholegrain breakfast cereals Kellogg’s, Ireland 2016 Contains inulin as source of fibre

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Chicory inulin & AXOS

ProDigest VIB Bio-incubator Gent | Technologiepark 4

B-9052 GENT (Belgium)

tel. +32(0) 9/241.11.90 | fax. +32(0) 9/241.11.91

Part 1 • Bacterial faecal samples of three male Gent students • Incubation in “simulated large intestine” • Evaluate the effect of (5 g/L): Inulin: Fibruline Instant AXOS: arabinoxylan-oligosaccharide-enriched

compound, from wheat arabinoxylan Control: colon medium

Sampling at 0, 24 & 48 h Endpoints: pH Gas production Short-chain fatty acid

Part 2 Effects of metabolite mix on colonic cells • Permeability • Viability • Immune markers

SHIME: IN-VITRO SIMULATOR HUMAN INTESTINAL MICROBIAL ECOSYSTEM

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PH

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GAS

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ACETATE

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PROPIONATE

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BUTYRATE

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Barrier integrity (TEER)

Inflammation (cytokines & chemokines)

Macrophages - THP1-B

Colonocytes - Caco-2

Lipopolysaccharide from E. coli

Intestinal lumen

Blood stream

MODEL INFLAMMATORY BOWEL DISEASE

↓ incubation mix with metabolites

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EFFECTS ON COLONOCYTES

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IMMUNE MARKERS

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PRINCIPAL COMPONENT ANALYSIS: 5 -> 2

Inulin

Ctrl

Na-Butyrate

BNC

Axos

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•Cosucra’s Fibruline®: prebiotic; stimulation of

gut bacteria -> metabolites e.g. acetate,

propionate & butyrate

•Impacted markers of immunity/inflammation &

improve intestinal gut barrier function.

•May moderate leaky gut resulting from e.g.

food intolerances, allergies, Crohn disease

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