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BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 1BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 1
Find the Targets and Answers that Matter
Introducing the AbsoluteIDQ® p400 HR Kit and Q Exactive™ Orbitrap™ MS for Targeted Lipid and Metabolic Profiling
For Research Use Only. Not for use in diagnostic procedures.
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 2BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 2
AbsoluteIDQ® p400 HR Kit
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
1. Background Information
2. AbsoluteIDQ® p400 HR Kit
- Metabolite Coverage
- Requirements
- Kit Components
- Kit Workflow
3. Analytical Performance
4. Biological Relevance & Applications
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 3BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 3
AbsoluteIDQ® p400 HR Kit
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
1. Background Information
2. AbsoluteIDQ® p400 HR Kit
- Metabolite Coverage
- Requirements
- Kit Components
- Kit Workflow
3. Analytical Performance
4. Biological Relevance & Applications
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 4For Research Use Only. Not for use in diagnostic procedures.
Biocrates Life SciencesCompany Profile
Globally operating biotech company based in Innsbruck, Austria
> 15 years of experience, with > 800 publications
Fully integrated mass spectrometry based solutions fortargeted metabolic phenotyping
Services & kits: quantitative analysis of > 800 endogenous metabolites, statistical analysis, and biological interpretation
Hypothesis driven biomarker research in pre-clinical, clinical, and epidemiological research
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 5BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 5
AbsoluteIDQ® Stero17 Kit*- mineralocorticoids- glucocorticoids- sex steroids
Targeted MetabolomicsKits for Standardized & Quantitative Metabolic Phenotyping
188
20
17
Metabolites
QQQ MS
408 AbsoluteIDQ® p400 HR Kit+ 220 lipids!
HRAMMS
Reprinted with permission of Thermo Fisher Scientific, CCL2012
*For US/Canada: License pending
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p180 Kit- amino acids, biogenic amines- acylcarnitines, hexoses- glycerophospho- and sphingolipids
Biocrates® Bile Acids Kit- primary bile acids- secondary bile acids- human and rodent specific bile acids
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 6BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 6
AbsoluteIDQ® p400 HR Kit
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
1. Background Information
2. AbsoluteIDQ® p400 HR Kit
- Metabolite Coverage
- Requirements
- Kit Components
- Kit Workflow
3. Analytical Performance
4. Biological Relevance & Applications
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 7BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 7
AbsoluteIDQ® p400 HR KitCoverage
For Research Use Only. Not for use in diagnostic procedures.
Some interesting metrics
HMDB metabolites: 390,000
Chemicals with 15N, 13C, 2H labeled standards: 400
Typical number of features in discovery untargeted platforms: 10,000
Compounds Identified: 200 – 1000?
Source: David Wishart, presentation at MSACL 2017 EU (Salzburg, Austria) September 2017
Biocrates p400HR – identified and quantifiable metabolites: 408
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 8BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 8
Key Benefits at a Glance
Standard Thermo Scientific™ Orbitrap™ HRAMMass Spectrometer (+ UHPLC)
AbsoluteIDQ® p400 HR KitMetabolic and Lipid Profiling
For Research Use Only. Not for use in diagnostic procedures.
408 Endogenous Metabolites
11 metabolite classes 365 lipids & 43 small molecules
Small Molecules- Amino acids (21)- Biogenic amines (21)- Hexoses (1)
Polar lipids- Phosphatidylcholines (172)- Lysophosphatidylcholines (24)- Sphingomyelins (31)- Ceramides (9)
Neutral lipids- Acylcarnitines (55)- Diglycerides (18)- Triglycerides (42)- Cholesteryl esters (14)
Q Exactive™ Focus
Q Exactive™
Q Exactive™ Plus
Q Exactive™ HF Reprinted with permission from Thermo Fisher Scientific, CCL2012
Ready-to-use quantitative kit forQ Exactive™ platforms
High-throughput &minimal sample volume
Simple & efficient automatedworkflow
Robust platform for longitudinal & inter-lab comparability
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 9BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 9
Easy & Streamlined Workflow
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 10BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 10
Unique Standardized & Quality Controlled
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
All in one box
Ready-to-use & user-friendly
Standardized & quality controlled
Only 10 µL sample required
Validated for human plasma
Kit Components
Reagents & Consumables: Patented 96-well filter plates
Internal standards
Calibration standards
Quality controls
Instrument suitability test mix
Methods & Protocols: Sample preparation protocol
Instrument-specific acquisition &
quantitation methods
Workflow Manager MetIDQ™ Process guidance
Automated technical validation
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 11BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 11
Workflow Manager MetIDQ™: Automated & Guided Process
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Transparent, Automated Workflow
1. Information Management Sample registration, plate layout design
Worklist generation
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 12BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 12
Workflow Manager MetIDQ™: Automated & Guided Process
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Transparent, Automated Workflow
1. Information Management Sample registration, plate layout design
Worklist generation
2. Quantification & Data Processing Automated signal identification & quantification
Data merging
3. Automated Technical Validation Transparent display of generated data
Analytical approval of the plate
Inter-plate monitoring of QC results and precision
4. Data Evaluation and Export Data normalization (optional)
Export of results in various file formats
5. Statistical Analysis (optional) Basic statistics & data visualization
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 13BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 13
Workflow Manager MetIDQ™: Automated & Guided Process
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Transparent, Automated Workflow
1. Information Management Sample registration, plate layout design
Worklist generation
2. Quantification & Data Processing Automated signal identification & quantification
Data merging
3. Automated Technical Validation Transparent display of generated data
Analytical approval of the plate
Inter-plate monitoring of QC results and precision
4. Data Evaluation and Export Data normalization (optional)
Export of results in various file formats
5. Statistical Analysis (optional) Basic statistics & data visualization
Transparent, automated & guided process
Provides quantitative results
Reduced complexity & process safety
Efficient & time saving
High confidence in results
MetIDQ™ Software
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 14BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 14
AbsoluteIDQ® p400 HR Kit
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
1. Background Information
2. AbsoluteIDQ® p400 HR Kit
- Metabolite Coverage
- Requirements
- Kit Components
- Kit Workflow
3. Analytical Performance
4. Biological Relevance & Applications
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 15For Research Use Only. Not for use in diagnostic procedures.
Proven Excellent AccuracyAbsoluteIDQ® p400 HR Kit
0
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Accu
racy
[%]
Accuracy Range 90-114% n = 3
NIST SRM 1950
“The field MUST become more quantitative if findings are to be translated to practical, clinical applications.”
David Wishart (2015)
http://www.wishartlab.com/system/images/W1siZiIsIjIwMTMvMDMvMDYvMTBfMzJfMDBfNjE2X3dpc2hhcnQuanBnIl1d/wishart.jpg
“If we could get over this barrier [to not quantitate our data] then it will make exchanging data between labs and studies so much easier, and we could really move forward as a community.”
Jules Griffin (MetaboNEWS, Sept 2017)https://www.bioc.cam.ac.uk/people/images/griffin.jpg/@@images/2b4df4dc-9f1a-4e50-a5f4-c2ce9a93f0a8.jpeg
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 16
2017-01-16_KIT2_QEF_Col250_SST_004 01/16/17 09:55:24 Testmix 986150
RT: 0.00 - 5.81
0 1 2 3 4 5Time (min)
0
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0
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3.99 4.283.93 4.891.47 5.422.750.36 1.97
0.18
0.34 4.892.730.82 1.31 3.923.502.01 5.211.31
1.67
1.81 5.024.69 5.312.09
2.022.27
4.04
1.944.60
5.493.172.832.321.620.17
NL: 7.90E9TIC MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 1.24E9m/z= 114.06562-114.06676 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 9.24E6m/z= 261.03490-261.03752 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 1.67E8m/z= 310.13167-310.13477 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 1.14E8m/z= 338.16283-338.16621 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 1.74E8m/z= 267.11484-267.11752 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 4.43E6m/z= 743.27638-743.28382 F: FTMS + c ESI Full ms [55.0000-800.0000] MS 2017-01-16_KIT2_QEF_Col250_SST_004
NL: 5.96E2Pump_Pressure A/D card 2017-01-16_KIT2_QEF_Col250_SST_004
B:\Biocrates\...\2017-01-17_KIT3_SST_004 01/17/17 08:43:13 p150 testmix in
RT: 0.00 - 2.81
0.0 0.5 1.0 1.5 2.0 2.5Time (min)
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ance 0
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1000.88 0.920.84
0.72 1.070.68 1.18
1.250.61 1.51 2.04 2.17 2.440.10
0.97
2.09 2.762.571.640.100.84 0.970.74
2.04 2.762.570.100.89 0.96
1.98 2.15 2.641.800.260.930.79
1.98 2.161.63 2.600.270.90 0.97
2.161.64 2.39 2.661.860.280.930.79
1.98 2.161.63 2.600.270.87 0.97
2.16 2.39 2.660.28
NL: 6.94E8TIC MS 2017-01-17_KIT3_SST_004
NL: 2.90E7m/z= 203.05159-203.05363 F: FTMS + c ESI Full ms [150.0000-220.0000] MS 2017-01-17_KIT3_SST_004
NL: 3.41E6m/z= 162.11169-162.11331 F: FTMS + c ESI Full ms [150.0000-220.0000] MS 2017-01-17_KIT3_SST_004
NL: 3.41E6m/z= 428.37126-428.37554 F: FTMS + c ESI Full ms [390.0000-520.0000] MS 2017-01-17_KIT3_SST_004
NL: 4.55E7m/z= 524.36848-524.37372 F: FTMS + c ESI Full ms [520.0000-634.0000] MS 2017-01-17_KIT3_SST_004
NL: 2.27E7m/z= 731.60254-731.60986 F: FTMS + c ESI Full ms [730.0000-931.0000] MS 2017-01-17_KIT3_SST_004
NL: 8.09E7m/z= 622.44109-622.44731 F: FTMS + c ESI Full ms [520.0000-634.0000] MS 2017-01-17_KIT3_SST_004
NL: 8.33E7m/z= 790.62805-790.63595 F: FTMS + c ESI Full ms [730.0000-931.0000] MS 2017-01-17_KIT3_SST_004
LC-MSFIA-MS
Accuracy 92-104%,
Precision (CV)4-15%
For Research Use Only. Not for use in diagnostic procedures.
Analytical Performance: Inter-day ComparabilityAbsoluteIDQ® p400 HR Kit
Accuracy81-110%,
Precision (CV)10-25%
Inter-day comparability
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 17
Resolution for LC-MS:70k (Q Exactive™ Focus)60k (Q Exactive™ HF)
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
LC-MS based metabolites
Accuracy 92-104%,
Precision (CV)4-15%
Accuracy81-110%,
Precision (CV)10-25%
Resolution for FIA-MS:70k (Q Exactive™ Focus)120k (Q Exactive™ HF)70k (Q Exactive™ Plus)
FIA-MS based metabolites
Outstanding Inter-Lab Comparability
LC-MS Part
3 labs, 5 instruments (Q Exactive™ Focus & HF)
Reference lab 1 (Q Exactive™ Focus)
6 individual human plasma samples
FIA-MS Part
3 labs, 6 instruments (Q Exactive™ Focus, HF & Plus)
Reference lab 1 (Q Exactive™ Focus)
6 individual human plasma samples
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 18
Excellent Analytical Performance
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Worldwide Unique Ring Trials
AbsoluteIDQ® p180 Kit 6 laboratories, 3 instruments
7.6% CV Siskos et al. (2017), Anal Chem, 89: 1
Biocrates® Bile Acids Kit 12 laboratories, 4 instruments
8.3% CV Pham et al. (2016), J Appl Lab Med,
DOI: jalm.2016.020537
First ring trial scheduled for AbsoluteIDQ® p400 HR Kit
Beger et al., Metabolomics (2016) 12: 149
“[…] Biocrates commercially supplies kits for analysis of targeted areas of metabolism. […] Each kit provides advantages in interlaboratory use […].”
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 19BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 19
AbsoluteIDQ® p400 HR Kit
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
1. Background Information
2. AbsoluteIDQ® p400 HR Kit
- Metabolite Coverage
- Requirements
- Kit Components
- Kit Workflow
3. Analytical Performance
4. Biological Relevance & Applications
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 20BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 20
Unique Analyte Coverage Provides Rich Information
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Biological Functions
Membrane composition
Cellular signaling
Mitochondrial functions- Mitochondrial beta oxidation of fatty acids- Bioenergetic processes
OncologyCardiologyNeurologyAgingDiabetes…
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 21
Multiple Rate-Limiting Enzymes Covered
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Rate-Limiting Step
Slowest step of an enzymatic reaction that determines the rate (speed) at which the overall reaction proceeds.
PLA2-Activity
Ratio lysoPC/PC lysoPC synthesis Inflammation
IDO-Activity
Ratio Kyn/Trp Tryptophan breakdown Immune modulation
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 22
Case Study: Advantage of Broad Metabolite Coverage
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Sample Description Sample Number
Healthy Controls 150
Ovarian Cancer Patients 69
Collaboration: Prof. Burwinkel,Heidelberg University, Germany
Identification of key metabolites of ovarian cancer in plasma
AA and AC are the top markers
Importance of affected pathways in tumor pathophysiology and other diseases
Unique metabolite portfolio of AbsoluteIDQ® p400 HR Kit provides added value
Component 1 (83.8%)
Com
pone
nt2
(16.
2%)
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 23
Metabolic Signatures: Newborn Screening
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Beger et al., Metabolomics (2016) 12: 149
Newborn screening
Phenylketonuria (PKU) Phe ↑, Phe/Tyr > 2.5
Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency C6 > 0.21, C8 > 0.32, C10 > 0.48, C10:1 > 0.28 C8/C10 > 1.6 or C8/C12 > 1.6
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 24
Val + Leu + IlePhe + Tyr + Trp
Metabolic Signatures: Precision Medicine
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Beger et al., Metabolomics (2016) 12: 149
BCAAAAA
Fischer ratio
↑ BCAA/AAA ratio with
↓ severity of liver failure
Fischer et al., Am J Surg (1974) 127: 40
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 25
The AbsoluteIDQ® p400 HR Kit is a ready-to-use solution designed for targeted metabolomics on Q Exactive Orbitrap HRAM MS. Ready to go within a day, without method development
The kit provides quantitative results for up to 408 metabolites covering a broad range of biological functions.
The kit delivers a standardized, quality controlled, and targeted assay for accurate and confident metabolic profiling.
The simple, efficient, and automated workflow ensures high throughput and use of minimal sample volume.
The robust platform with standardized procedures assures longitudinal and inter-lab comparability.
Summary & Conclusions
For Research Use Only. Not for use in diagnostic procedures.
AbsoluteIDQ® p400 HR Kit
Standardized and Reproducible Quantitation of Hundreds of Lipids & Small Moleculesfor Broad Lipid and Metabolic Profiling
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 26BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 26
Acknowledgements
BIOCRATES Pham Tuan HaiTherese KoalDoreen KirchbergMartin BurattiSimon SchaffererKristaps KlavinsBarbara WolfBrigitte PfurtschellerMarkus LangsdorfManuel KratzkeMatthias SchefflerAnton GronesWulf Fischer-Knuppertz
For Research Use Only. Not for use in diagnostic procedures.
Thermo Fisher ScientificAndreas HuhmerAnastasia KalliMadalina OppermannClaudio DeNardiDavid PeakeReiko KiyonamiGlenn DamkroegerDirk BrouwerNeli JochimHeather SumnerKlaus Lindpaintner
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 27BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 27
Thank you!Contact us for more information
For Research Use Only. Not for use in diagnostic procedures.
Helen.Karuso@biocrates.comStephen.Dearth@biocrates.com
www.biocrates.com
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 28BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 28
Universal Selling Points of p400 HRHow to transport the messages?
Feature USP / Benefit
Standard Instrumentation No additional investments required
Broad Installed base Easy accessibility
408 endogenous metabolites from 11 metabolite
classes - 365 lipids & 43 small molecules
Unique broad Metabolic and Lipid Coverage gaining insights in to Cell Signaling & Structural Information + Bioenergetics (Acyl-carnitine, AS & Biogenic Amines)
Complete Standardized & Quality controlled All-in-one Solution
Ready to use & user friendly (No instrument tuning and method development)
transparent streamlined Workflow Manager MetIDQ guided process High confidence into analytical results
Automated Signal identification, -quantification and technical validation High confidence into analytical results
96-well plate format, solid phase mediated sample preparation High-throughput, multiplexing, small sample volume
Quantitative Results with a High Accuracy, Intraday comparability & multi center + instrument reproducibility
Robust Platform necessary for Longitudinal & Inter-lab/study comparability
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 29
SystemicAnalysis
For Research Use Only. Not for use in diagnostic procedures.
Metabolic PhenotypingSurrogate Marker for Individual Phenotype
Disposition
Immune system Blood group Gender Age
Acquired
Metabolic homeostasis(compensation mechanism)
Immune response- B cell response- T cell response
Somatic Changes
Genetic alteration Epigenetic regulation Transcriptional regulation
Exposure
External pathogens Microbiota Lifestyle & Nutrition Environment Drug treatment
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 30BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 30
Overcoming Challenges of Untargeted MetabolomicsTargeted Metabolomics
Instrument 2
Absolute Concentrationsstandardized & reproducible
For Research Use Only. Not for use in diagnostic procedures.
Untargeted Metabolomics
~ 2 months
Instrument 1 Instrument 2Instrument 1
Software- Workflow Manager- Methods & Protocols- Signal Translation
Reagents- Standards- Controls
Biocrates Kits
Consumables- Kit Plate
Data Can Be Pooledlab to lab, study to study, longitudinal
Unidentified Features
Peak Interpretation
Signal Annotation (Database)
Fold ChangesNOT standardized & NOT reproducible
Data NOT comparableNOT lab to lab, NOT study to study, NOT longitudinal
80 samples
1 PhD scientist
100-400 Metabolites
30 hours
1 Technician
400 Metabolites
Targeted Metabolomics
BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria 31
Cardiology
SysVasc: Identification of molecular targets for vascular disease treatment
BiomarCaRE:Biomarkers for cardiovascular risk assessment
Biocrates is Partner of ChoiceLarge Scale, Long Term Studies
Lifestyle & Genomics
HELIX: Impact of early environmental influences on health (incl. 6 European birth cohorts)
Diabetes
DeTecT2D: Development of a new diagnostic tool for early detection of type 2 diabetes
Nephrology
SysKid: Understanding chronic kidney disease (diabetes and hypertension context)
Neurology
BrainAge: Impact of prenatal stress on brain ageing
ADNI (1, GO, 2):Understanding of Alzheimer's disease and early diagnosis
Nutrition & Lifestyle
Nutritech: Effect of diet on “phenotyping flexibility”
Epidemiological Cohorts
KORA (F3, S4, F4) UK Twins EPIC CHRIS Leiden Longevity
Study PopGen ERF Fenland Others