Post on 26-Nov-2014
description
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visualizing Gel Electrophoresis
flatbed scanner+
UV transilluminator
gel stained with Biotium GelRed
LED light guide (FTIR?)
linear CCD array
LED light guide (FTIR?)
linear CCD array
linear CCD array, upside down
RGB LED (3 diodes)
RGB LED couples here
red solid state laser light through light guide rail
welding curtain: UV filter
fitting UV curtain over
CCD array
FOTODYNE UVB transilluminator (circa 1994)
glass (UV-opaque)
special UV-transparent polycarbonate
stained gel
UV-glass(visible-opaque)
transilluminator
glass (UV-opaque)below gel
welding curtain on top of gel
365nm handheld lamp
exposure: 1/4 sec
exposure: 5/8 sec
exposure: 1 sec
exposure: 2 sec
exposure: 4 sec
exposure: 8 sec
contrast enhanced
365nm handheld lamp
~365 nm
visualized w/ ambient light
visualized w/ ambient light
visualized w/ UV light from fotodyne
imaged w/ Lumix GF1 SLR cameraimaged w/ modifed scanner
darklight!
using small UVB lamp inside the scanner
next steps:add low-cost filter to linear CCD
GelGreen:Excitation Peak: ~500 nmEmission Peak: ~540 nm
Need to filter emission light before linear CCD- find a filter just above the light source but below the emission- 470-510 nm < filter < 540 nm- TAPR50 sheets filter everything below 528 nm
get TAPR50 528nm filter sheets($35 / 10 sq feet)
http://bit.ly/TAPR50