Post on 26-Mar-2015
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Genomics for Triticeae improvement
FP7 European Project
OverviewOverview
Achieve significant progress in Triticeae genomics and support efficient
breeding of improved varieties for European agriculture
European Seventh Framework Programme project (financed by the EC)
Project duration: 4 years – From June 2008 to Mai 2012
Budget: 7,5 million Euro (5,3 million Euro supported by the EC)
Project Coordinator: Catherine Feuillet (INRA)
Activities: 5 research WPs, 1 WP for project dissemination and 1 WP for
project management
OverviewOverview 17 partners (including 2 breeders) from 9 countries
Public
Private
Activities and WPsActivities and WPs
WP1: Physical mappingWP1: Physical mapping
Of wheat group 1 and 3 chromosomes
Construction of BAC libraries
Fingerprinting of BAC libraries
Assembly of BAC contigs
BAC libraries pooling from the MTP and rearraying for
BES (by INRA-CNRGV)
BAC end sequencing of the MTPs for marker
development and genome analysis
WP1: Physical mappingWP1: Physical mapping
Assembly of BAC contigs
• Chromosome 3B: assembly by INRA (France)
• Chromosome 3D: assembly of 3DS by IEB (Czech Republic),
and assembly of 3DL by JIC (UK)
• Chromosome 1B: assembly of 1BS by HU (Israel), and
assembly of 1BL by INRA (France)
• Chromosome 1A: assembly of 1AS by UZH (Switzerland), and
assembly of 1AL by SU (Turkey)
WP2: Physical maps anchoringWP2: Physical maps anchoring
Of wheat and barley group 1 and 3 chromosomes
Establishment of a marker repository: including only markers for
which sequences are available
Generating resources for high resolution genetic mapping: For
wheat, creation of a population of CS x Re RILs of about 2000
plants. For barley, creation of Morex x Barke RILs.
Anchoring BAC contigs to chromosomes by high throughput
screening methods: forward and reverse anchoring
Comparative genetic and physical mapping with other grass
genomes
WP3: WP3: Gene isolation and new alleles discoveryGene isolation and new alleles discovery
Isolate five agronomically important genes and QTLs (4 in wheat and 1
in barley)
Trait Species Chr. Partner
Stripe rust resistance YrH52 Wild emmer wheat
1BS HU
Wheat quality (pentosan viscosity) PV-QTL
Bread wheat 1BL INRA
Glume blotch resistance
QSng.sfr–3BS
Bread wheat 3BS UZH
Grain yield QYld.idw-3BS Durum wheat 3BS UNIBO
Plant architecture (tillering) cul4
Barley 3H UMIL
WP3: WP3: Gene isolation and new alleles discoveryGene isolation and new alleles discovery
High-resolution mapping of the genes underlying the
target traits
Identification of candidate genes
Functional validation of candidate genes
New alleles discovery
WP4: Molecular markers for breedingWP4: Molecular markers for breeding
WP leading by the breeding companies
Development of trait specific markers: using markers from
WP1 (BAC end sequencing) and WP2
Establishment of an European diversity panel for
association mapping: panel contains 730 varieties (171 from
UK, 178 from Germany and 381 from France)
The data generated will be useful for all the chromosomes.
WP5: WP5: Bioinformatics tools and databasesBioinformatics tools and databases
For wheat and barley
Development of a web interface integrating genetics and physical
maps
Development of a new analytical framework for contig assembly:
LTC (more robust than FPC)
Development of comparative genome informatics tools: with
Brachypodium, rice, sorghum, etc…
Development of a new version of TriAnnot pipeline for automated
sequence annotation
WP6: WP6: Dissemination, technology transfer and trainingDissemination, technology transfer and training
Organisation of 9 training courses in the different
partners instituts
Development of the Project website
http://www.triticeaegenome.eu/
Dissemination at scientific and technological events:
talks/posters, production of a project leaflet and a poster
WP7: Project ManagementWP7: Project Management
Scientific and technical management
Administrative and financial management: organization of
the different project meetings, monitoring of the project costs
Communication and interactions between partners and
other projects: establishment of a Collaborative Platform,
interaction with the Triticeae Genomics Network (TGN)
Technical and financial reporting to the EC: detailed
reports that describe the work progress, costs incurred and other
justifications for the reimbursement of project costs
UC Davis
Kansas SU
CSIC
Viterbo U
Modena U
CRA-CER Foggia
Murdoch U CSIRO
UC Riverside
Iowa SU
Okayama U
Minnesota SU
CAAS Beijing
CIMMYT
MPI Cologne
ACPFG
A large network of international collaborators
INTA
UMB