Post on 10-Oct-2015
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Microbiology laboratoric test
Gram StainMaterialsolutions of crystal violet, Grams iodine (2 g potassium iodide in 300 ml distilled water plus 1 g iodine crystals), 95% ethanol and/orisopropanol-acetone mixture (3:1 v/v), andsafraninBismark brown stain (for color-blind students)clean glass slidesinoculating loopBunsen burnerbibulous papermicroscopelens paper and lens cleanerimmersion oilHyphomonas (Hyphomicrobium) neptunium (ATCC 15444) grown in marine broth (Difco)slide warmerstaining rackBacto Gram Stain Reagents from Difco for the three-step Gram stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram StainExamination the preparation under microscope ( x100) for confirmation
Microscopic result for Gram Stain
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