Post on 08-Aug-2015
transcript
Downstream Processing of Recombinant and EndogenousProteins from Livestock Milk
byArthur W. Degener, Jr.Faculty of the Virginia Polytechnic Institute and State University
Background
Introduction
Introduction
With the Increased demands of therapeutic proteins, there is going to be a need for new purification technologies which have
high throughputhigh yield
high resolution
Introduction
Three purification technologies were explored as potential new technology to isolate recombinant and endogenous milk proteins
1. Expanded bed adsorption chromatography(EBAC) combined with hydrophobic interaction chromatography(HIC)
2. Recycle continuous flow electrophoresis(RCFE) 3. Free flow isoelectric focusing(FFIEF).
Introduction
The first process(EBAC/HIC) used with Zn2+ as a selective precipitating agent, purified recombinant human protein C (rhPC) and IgG (contaminated with less than 1% IgA) from swine milk with high resolution and high yield while processing about 10-20 grams in a single operation.
1. Expanded bed adsorption chromatography(EBAC)
Dynamic light scattering (DLS) measurements
Dynamic light scattering (DLS) measurements
2. Hydrophobic interaction chromatography(HIC)
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