Lab 6

THE RESPIRATORY SYSTEM & SPIROMETRY

LAB 6 ACTIVITIES• Identify parts of the respiratory system

• Respiratory tree, thoracic organs, and larynx models

• Fresh lung (if available)

• Examine histology of the:• Trachea

• Lung

• Examine the operation of the lung model

• Biopac L012-Lung-1: Spirometry

• Measure lung volumes and capacities

RESPIRATORY SYSTEM STRUCTURE1. Conduction zone:

pathway for pulmonary ventilation

2. Respiratory zone: membrane for gas exchange external respiration

• Clinically, two parts:• Upper respiratory tract

• Lower respiratory tract

RESPIRATORY SYSTEM STRUCTUREHistology reflects the different

functions of the different parts of the system

Position in respiratory pathway determines cell type

• non-keratinized stratified squamous

• pseudostratified ciliated columnar

• cuboidal ciliated

• simple squamous / Type I Alveolar cells

PSEUDOSTRATIFIED COLUMNAR EPITHELIUM

Lamina Propria

Hyaline Cartilage

mucosa

Submucosa

seudostratified epithelium

= lamina propria

ucous glands

mooth muscle

artilage

GROSS SECTION THROUGH LUNG

SMOKER’S LUNGS Non-smoker

SMALL BRONCHIOLE

LUNG TISSUE SLIDE Respiratory Bronchiole

Alveolar Duct

Alveoli

Alveolar Sac

CAPILLARIES IN ALVEOLAR WALL

SPIROMETRY

Total Lung Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) + Residual Volume (RV)

Vital Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV)

Inspiratory Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV)

Functional Residual Capacity = Expiratory Reserve Volume (ERV) + Residual Volume (RV)

SPIROMETRY – RESPIRATORY VOLUMES

AND CAPACITIES

SPIROMETRY: CALIBRATION

Insert the calibration syringe/filter assembly into the side of the airflow transducer labeled "Inlet." 

Inlet

Follow pop up window instructionsWait for 8 seconds of no air flow,Then do your 5 syringe cycles.

Inlet

EXPERIMENTAL TREATMENTS

1. 4 normal breaths

2. Inhale as deeply as possible

3. Exhale to normal depth

4. 3 normal breaths

5. Exhale as much as possible

6. 3 normal breaths

7. Inhale as much as possible

8. Exhale as fast and completely as possible

9. 4 normal breaths

BIOPAC SPIROGRAM EXAMPLE DATA

3 regularbreaths

3 regularbreaths

3 regularbreaths

1 strongestinhale

1 regular exhale

1 strongestinhale

1 strongest exhale

1 strongest exhale

This part is in addition to what Biopac Lesson 12-Lung 1 prompts you to do!

If you get a negative value, just put the absolute value.

BIOPAC: DATA ANALYSIS

P-P Measures Tidal VolumeRecord in Table 1 on page Lab 6-19

BIOPAC: DATA ANALYSIS

P-P Measures Inspiratory CapacityRecord in Table 1 on page Lab 6-19

BIOPAC: DATA ANALYSISDelta Measures Inspiratory Reserve VolumeRecord in Table 1 on page Lab 6-19

Use similar methods to determine:Expiratory Reserve VolumeVital Capacity

BIOPAC: DATA ANALYSISTo measure FEV1

Highlight from peak of max inhale to the point where Delta T is 1 secondP-P Measures FEV1

Record in Table 1 on page Lab 6-19

Use similar methods to determine FEV2 and FEV3:

BIOPAC: DATA ANALYSIS

P-P Measures FVCRecord in Table 1 on page Lab 6-19

SUMMARY OF CLASS DATA

Record your data in Excel at the computer located at the front podium

Your Instructor will make the complete data set available for you to use while completing the homework assignment

HOMEWORK

Complete and turn in the questions on pages 6-19 to 6-22

Don’t forget to print the entire spirogram (landscape format) you recorded to include in your lab report.

Use vertical brackets ( [ ] ) to identify and label the tidal, inspiratory reserve, expiratory reserve and residual volumes; and total lung, vital, expiratory and inspiratory capacities on your spirogram.

Lab 6-21, Question 4. Use a scatterplot, not a line graph! Remember to completely label your graphs!

Complete Assignment 6 on MasteringAandP.

Lab 6

THE END

BIOPAC SPIROMETRY

Residual volume

SPIROMETRY