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RAMA UNIVERSITY UTTAR PRADESH, KANPUR
Faculty of Engineering & Technology
SYLLABUS & EVALUATION SCHEME
[Effective from the Session 2015-16]
M. Tech. Biotechnology
1st & 2nd year
RAMA UNIVERSITY UTTAR PRADESH, KANPUR
A meeting of the Board of Studies of the department of Biotechnology of Faculty of Engineering and
Technology Rama University Uttar Pradesh, Kanpur was held on 5th
July 2014 at 11 AM. The following
members were present:
1. Dr. Ajay Kumar Convener
2. Mr. Ajit Pratap Singh Yadav Member
3. Mr. Vachaspati Rao Member
4. External Member
5. External Member
The quorum of the meeting was complete.
Agenda of the meeting:
1. Assessment Criteria
2. Question Paper Format
3. Syllabus
The meeting resolved unanimously that attached Assessment Criteria, Question Paper Format and Syllabus
are justified and approved.
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
Date:
Faculty of Engineering & Technology Rama University Uttar Pradesh, Kanpur
Course Detail and Evaluation Scheme (Effective from the Session 2015-16)
M. Tech. Biotechnology First Year
SEMESTER-I
S. N. Subject
Code Name of the Subject
Periods Evaluation Scheme Total
Marks Credit
L T P CE MTE ETE
Theory Subject
1 MTB 101 Advanced Biochemistry
3 1 0 20 30 100 150 4
2 MTB 102 Cell Biology & Microbiology
3 1 0 20
30 100 150 4
3
MTB 103 Biophysical Tools &
Techniques 3 1 0 20 30 100 150 4
4
MTB 104 Bioprocess & Fermentation
Technology 3 1 0 20 30 100 150 4
Practical
5 MTB 151 Biochemistry Lab
0 0 3 20 30 50 2
6
MTB 152 Cell Biology & Microbiology
Lab 0 0 3 20 30 50 2
TOTAL
12 4 6 700 20
L-Lecture, T-Tutorial, P- Practical, CE- Continuous Evaluation, MTE-Mid Term Examination, ETE-End Term Examination
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
Faculty of Engineering & Technology Rama University Uttar Pradesh, Kanpur
Course Detail and Evaluation Scheme (Effective from the Session 2015-16)
M. Tech. Biotechnology First Year
SEMESTER-II
S. N. Subject Code Name of the Subject Periods Evaluation Scheme
Total Marks
Credit L T P CE MTE ETE
Theory Subject
1
MTB 201 Advanced Genetics &
Molecular Biology 3 1 0 20 30 100 150 4
2 MTB 202 Immunology
3 1 0 20
30 100 150 4
3 MTB 203 Bioinformatics
3 1 0 20 30 100 150 4
4
MTB 204-
MTB 207
Departmental Elective- I
3 1 0 20 30 100 150 4
Practical
5
MTB 251 Molecular Biology &
Immunology Lab 0 0 3 20 30 50 2
6 MTB 252 Bioinformatics Lab
0 0 3 20 30 50 2
TOTAL
12 4 6 700 20
L-Lecture, T-Tutorial, P- Practical, CE- Continuous Evaluation, MTE-Mid Term Examination, ETE-End Term Examination
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
Faculty of Engineering & Technology Rama University Uttar Pradesh, Kanpur
Course Detail and Evaluation Scheme (Effective from the Session 2015-16)
M. Tech. Biotechnology First Year
SEMESTER-III
S. N. Subject Code Name of the Subject Periods Evaluation Scheme
Total Marks
Credit L T P CE MTE ETE
Theory Subject
1
MTB 301-
MTB-304
Departmental Elective- II
3 1 0 20 30 100 150 4
2
MTB 305-
MTB-308
Departmental Elective- III
3 1 0 20
30 100 150 4
Practical
1 MTB 351 Mini Dissertation
0 0 16 100 400 500 12
TOTAL
6 2 16 800 20
L-Lecture, T-Tutorial, P- Practical, CE- Continuous Evaluation, MTE-Mid Term Examination, ETE-End Term Examination
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
Faculty of Engineering & Technology Rama University Uttar Pradesh, Kanpur
Course Detail and Evaluation Scheme (Effective from the Session 2015-16)
M. Tech. Biotechnology First Year
SEMESTER-IV
S. N. Subject Code Name of the Subject Periods Evaluation Scheme
Total Marks
Credit L T P CE MTE ETE
1 MTB 451 Dissertation
0 0 24 200 600 800 20
TOTAL
0 0 24 800 20
L-Lecture, T-Tutorial, P- Practical, CE- Continuous Evaluation, MTE-Mid Term Examination, ETE-End Term Examination
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
DEPARTMENTAL ELECTIVES
Departmental Elective-I:
1. MTB 204 Advanced Environmental Biotechnology
2. MTB 205 Plant Biotechnology
3. MTB 206 Food Biotechnology
4. MTB 207 Nanobiotechnology
Departmental Elective-II:
1. MTB 301 Advanced Genetic Engineering
2. MTB 302 Bioreactor Engineering
3. MTB 303 Genomics & Proteomics
4. MTB 304 Animal Biotechnology
Departmental Elective-III:
1. MTB 305 Pharmaceutical Biotechnology
2. MTB 306 IPR, Bioethics and Biosafty
3. MTB 307 Research methodology in Biotechnology
4. MTB 308 Enzyme Technology & Industrial Applications
Syllabus
MTB 101: Advanced Biochemistry
L T P Credit: 4
3 1 0
UNIT 1
Water - Structure, unusual properties, role in biological processes. Ionization of Water, pH scale. Buffers
and buffering mechanism, Buffering against pH Changes in Biological Systems: Phosphate buffer,
Bicarbonate buffer, Protein buffer, Amino acid Buffer & Hemoglobin Buffer System.
UNIT II
Carbohydrates – classification, structure and functions of monosaccharides, disaccharides and
polysaccharides. Ring structure and mutarotation, stereo isomers and structural isomers. Metabolism –
Glycolysis & oxidation of Pyruvate, TCA cycle, Gluconeogenesis, Pentose Phosphate Pathway, Oxidative
phosphorylation, Disorder/ diseases of carbohydrate metabolism.
UNIT III
Fats and lipids – Classification, structure and function: Simple, Compound lipids, Essential fatty acids.
Fatty acid synthesis, origin of acetyl-Co A for fat synthesis, Elongation & desaturation of Fatty Acids.
Activation & transport of fatty acid from cytosol to mitochondria for oxidation. Oxidation of saturated &
unsaturated fatty acids. Β-oxidation. Formation and utilization of ketone bodies. Disorder/ diseases of lipid
metabolism. [
UNIT IV
Amino acids and proteins - Classification & structure of amino acids. Essential amino acids. Peptide bond
formation, Ramachandran plot, Primary, secondary, tertiary & quaternary structure of proteins. Biosynthesis
of amino acids from intermediates of Citric Acid Cycle & other major pathways. Biodegradation of amino
acids: Deamination, transamination. Urea Cycle. Disorder/ diseases of amino acids metabolism.
UNIT V
Purines and pyrimidines – Structure and properties. Metabolism of Nucleotides: Purines & Pyrimidines
synthesis : de Novo & salvage pathway, Conversion of nucleoside monophosphates to nucleoside di and
triphosphates, Formation of deoxyribonucleotides. Catabolism of Purine and Pyrimidine nucleotides.
Disorder of purines and pyrimidines metabolism.
Text/Reference Books:
1. Harper’s Illustrated Biochemistry, (26th Edition) – R.K. Murray, D.K. Garner, P.A. Mayers & V.W. Rockwell, Pub:
McGraw Hill International Edition.
2. Principles of Biochemistry (4th Edition) – Lehninger, Nelson & Cox. Pub: Macmillan
3. Biochemistry (3rd Edition) – G. Zubay., Pub: Wm. C. Brown Pub.
4. General Biochemistry (5th Edition, 1996) – Weil, Pub: New Age Intl. Ltd.
5. Biochemistry (5th Edition) – Lubert Stryer. Pub: W.H. Freeman & Com., NY.
6. Biochemistry – D. Voet and J.G. Voet Pub: John Willy & Sons
7. Biochemistry (4th Edition, 1974) – West & Todd Pub: Oxford IBH,
MTB 102: Cell Biology & Microbiology
L T P Credit: 4
3 1 0
UNIT I
Structural and functional features of cell organelles. Cell cycle and regulation, cell aging, apoptosis.
UNIT II
Morphology and classification of eubacteria, archeaebacteria and viruses. Culture media. Cell cultivation
system. Isolation and identification of microbes, culture techniques. Preservation of cultures.
UNIT III
Physical and chemical methods for the control of microbes. Sterilization, Microbial growth kinetics. Batch
and synchronous growth.
UNIT IV
Biological nitrogen fixation,aerobic and anaerobic N2 fixing bacteria. Biofertilizers. Bacterial genetics:
transformation, conjugation, and transduction
UNIT V
Applications of microbiology. Environmental; Microbiology of domestic water and waste water. Microbes
in bioremediation. Different microbial disorders their causative pathogen, diagnosis, treatment and control:
Tuberculosis, Typhoid, Tetanus, AIDS, Rabies, Polio, Diarrhoea, Ascariasis
Text/Reference Books:
1. Microbiology –an introduction by Tortora ( Pierson education Publication)
2. Industrial microbiology by Prescott and Dunn
3. Microbiology by Pelczar (W C Brown publication)
4. Genral Microbiology by stainer ( Mac Millan Publication)
5. Microbiology by Pawar and Dagniwala ( Himalaya publishing House)
MTB 103: Biophysical Tools & Techniques
L T P Credit: 4
3 1 0
UNIT I
Microscopic techniques for observing cell structure: Principles and applications of Microscopy Light microscopy:
Bright field, Dark field, phase contrast, fluorescence microscopy, scanning and transmission electron microscopy,
flow cytometry.
UNIT II
Chromatographic Techniques: Types of chromatography: Planar(TLC and Paper chromatography) Column
chromatography and its type - Gel permeation, Ion exchange, Hydrophobic, Reverse-phase and Affinity
chromatography; HPLC and FPLC.
Electrophoretic techniques: Theory and application of Polyacrylamide gel electrophoresis and Agarose gel
electrophoresis; Capillary electrophoresis; 2D Electrophoresis; SDS PAGE.
UNIT III
Centrifugation: Basic principles; Mathematics & theory (RCF, Sedimentation coefficient etc); Types of centrifuge -
Microcentrifuge, High speed & Ultracentrifuges; Preparative centrifugation; Differential &density gradient
centrifugation, Analytical centrifugation.
Unit IV
Spectroscopic Techniques:Basic principles and application of NMR, ESR, Mass spectroscopy, UV- Visible
spectroscopy. X-ray diffraction. Nanoparticles; their application in medicine and biology.
UNIT V
Radioactivity: Radioactive & stable isotopes, Pattern and rate of radioactive decay, Units of radioactivity.
Measurement of radioactivity: Geiger-Muller counter, Solid & Liquid scintillation counters (Basic principle,
instrumentation & technique), autoradiography. Applications of isotopes in biochemistry.
Text/Reference Books:
1. Biophysical Chemistry vol. I, II & III (1997) Cantor and Schimmel Pub: W.H. Freeman
2. Molecular Biology of the gene (5th Edtion), – J.D. Wastson, T.A. Baker, S.P. Bell,
A. Gann, M. Levine, R. Losick, Pub: Pearson Education (Singapore) Pvt. Ltd. Delhi
3. Biochemistry (3rd Edition) – G. Zubay., Pub: Wm. C. Brown Pub
4. Biochemistry (2nd Edition) – D. Voet and J.G. Voet Pub: John Willy & Sons.
5. Physical Biochemistry (2nd Edition) D. Friefelder Pub: W.H. Freeman & Com.
6. Biochemistry (5th Edition) – Lubert Stryer. Pub: W.H. Freeman & Com., NY.
7. Principles of Biochemistry (4th Edition)–Lehninger, Nelson & Cox. Pub: Macmillan Pub.
8. Molecular Cell Biology, (5th Edition) H. Lodish, A. Berk P. Matsudaira Chris A. Kaiser, M.Krieger.
9. Practical Biochemistry (5thEdition)–K.Wilson & J.Walker.Pub:Cambridge Univ.Press, (U.K.)
MTB 104: Bioprocess & Fermentation Technology
L T P Credit: 4
3 1 0
UNIT I
Introduction to bioprocesses :Historical development of bioprocess technology, an overview of traditional and
modern applications of biotechnological processes, general requirements of fermentation processes, basic design and
construction of ferment or , main parameters to be monitored and controlled in fermentation processes.
UNIT II
Metabolic stoichiometry and energetic: Stoichiometry of cell growth and product formation, elemental balances,
degree of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product
formation, maintenance coefficients, energetic analysis of microbial growth and product formation, oxygen
consumption and heat evolution in aerobic cultures.
UNIT III
Media design for fermentation processes :Medium requirements for fermentation processes, oxygen requirements,
medium formulation of optimal growth and product formation, examples of simple and complex media, Medium for
plant cell and animal cell culture, medium design of commercial media for industrial fermentations- Plackett burman
design, response surface methodology, simplex design, continuous cultivation method to determine the kinetic
parameters and maintenance coefficient and pulse & shift method of medium optimization. Case studies on each
medium design methods.
UNIT IV
Kinetics of microbial growth and product formation :Phases of cell growth in batch cultures, fed batch and continuous
cultures, simple unstructured kinetic models for microbial growth, Monod model, growth of filamentous organisms &
yeast , growth associated and non growth associated product formation kinetics, Leudeking –piret models, substrate
and product inhibition on cell growth and product formation.
UNIT V
Fermentation Technology: Case studies on production of lactic acid , glutamic acid, penicillin, microbial lipase and
protease, recombinant insulin. Case studies should deal with strain improvement, medium designs, process
optimization etc.
Text/Reference Books:
Bailey ,J.E. and Ollis, D.F. Biochemical engineering fundamentals, 2nd
ed.,McGraw Hill ,1986.
Shuler,M.L. and Kargi,F. Bioprocess engineering: basic concepts, 2nd
ed.,Prentice-Hall,2002.
Doran Pauline M, Bioprocess engineering principles, Academic Press, 1995.
Stanbury,P.F.,Stephan J. Hall & A.Whitaker. Principles of Fermentation technology, Science&
technology books.
MTB 151: Biochemistry Lab
L T P Credit: 2
0 0 3
1. Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions
2. Quantitative estimation of carbohydrates
3. Distinguish reducing and non-reducing sugars
4. Quantitative estimation of proteins
5. Estimation of nucleic acids
6. Isoelectric precipitation
7. Separation of sugars, fatty acids and amino acids by paper chromatography
8. Extraction of lipids from plant material
9. Thin layer chromatography and paper chromatography.
MTB 152: Cell Biology & Microbiology Lab
L T P Credit: 2
0 0 3
Cell Biology Lab
1. Microscopy
2. Identification and staining of different types of cells.
3. Measurement of various Cell Organelles.
4. Detection of Mitosis with the help of microscope.
Microbiology Lab
1. Isolation and enumeration of microorganisms of soil by serial dilution-agar Plating Method
2. Acid fast staining of bacteria by Zeil-Neelsen Method.
3. Bacterial capsule and spore staining.
4. Biochemical test(fermentation of carbohydrates, catalase, amylase and peroxidase test)
5. To study the growth curve of bacteria at 370C with shaking at 150 rpm.
6. To study the effect of temperature on growth of given microbe.
REFERENCE BOOKS
1. Practical Manual of biotechnology: Mahajan R, Sharma J, Mahajan R.K.
2. Experiments in Microbiology, Biotechnology and Plant Pathology: Aneja K.R.
MTB 201: Advanced Genetics & Molecular Biology
L T P Credit: 4
3 1 0
UNIT I
Fundamental principles of genetics, gene interaction, multiple alleles, complementation, linkage,
recombination and linkage mapping, Extra-chromosomal inheritance, Chromosomes basis of heredity, Sex
determination, Sex linked, Sex limited and sex influenced inheritance, crossing over.
UNIT II
Gene structure, DNA & RNA as a genetic material, Griffith’s experiment, Hershey- chase experiment, Semi
conservative mode of DNA replication, Central Dogma of Molecular Biology, DNA replication in
Prokaryotes and Eukaryotes, DNA Damage & Repair, Cell cycle and regulation.
UNIT III
Transcription (Prokaryotes and Eukaryotes): Synthesizing mRNA from a DNA template, transcription unit,
substrate for transcription, transcription apparatus, RNA polymerases, Role of sigma factor in transcription,
role of promoters and enhancers. Post transcriptional modification process: end modification (5’ cap, 3’
poly A tail addition, splicing, RNA editing), Activity of DNA polymerases and Topoisomerases, Reverse
transcriptase.
UNIT IV
Genetic code: properties of genetic code, Second genetic code, Wobble hypothesis, Translation
(Prokaryotes and Eukaryotes), Post Translational Modification Process.
UNIT V
Gene regulation in viruses, prokaryotes (lac operon and trp operon) and Eukaryotes.
Text/Reference Books:
1. Albert B, Bray Denis et al.: Molecular Biology of The Cell, latest ed.
2. Watson, Hopkin, Roberts et al.: Molecular Biology of the Gene, 4 th ed.
3. Genetics- Strickberger, 2 nd.
4. Baltimore- Molecular Biology of the Cell.
5. Advance Genetics by G.S. Miglani, Narosa Publishing House
6. Essentials of Molecular Biology 4thed, Malacinski, G. M. Jones & Bartlet publishers, Boston
7. Genetics A molecular approach 2ndEdition Peter J. Russell Pearson.
8. Concepts of Genetics seventh edition William S.Klug and Michael R. Cummings, Pearson.
MTB 202: Immunology
L T P Credit: 4
3 1 0 UNIT I
Concepts of immunology: General principles of immune system, molecules, cells and tissues of immune
system, primary and secondary lymphoid organs (thymus, bursa of fabricius, lymph nodes, spleen), B and T
lymphocyte and their functions, lymphocyte cell mediated cytotoxicity.
UNIT II
Antigens and antibodies: Concepts of antigen, antigenic determinant, antigenicity, immunogen and
immunogenicity, factors affecting antigenicity, hapten, carrier effect, cross reactivity, adjuvtants, Freund’s
adjuvants and its significance, immunoglobulin, structure of immunoglobulin, types and properties of
immunoglobulin, theories of antibody formation, clonal selection, Ig genes, immunoglobulin synthesis and
metabolism, and antibody diversity.
UNIT III
Humoral and cell mediated immunity: MHC, MHC antigen- Class I, Class II, Class III, antigen
presentation, MHC restriction, immune response gene, immune response, humoral and cell mediated
immune response, BCR, TCR & generation of biodiversity, lymphocytes, T cells regulation, graft rejection.
UNIT IV
Antigen- antibody reaction: Physico-chemical basis of Ag-Ab interaction, avidity, strength of binding
between Ag and Ab and its measurement, detection of Ag-Ab interaction, precipitation, agglutination and
complement fixation, complement system, and cytokines. Hyper-sensitivity, Autoimmunity, Cancer, AIDS
and Transplantation immunology.
UNIT V
Vaccine technology&immunotechniques: Basic principles of vaccine development; protein based vaccines;
DNA vaccines; Plant based vaccines; recombinant antigens as vaccines; reverse vaccinology.
ELISA,Direct, indirect and Sandwich immunofluorescence, hybridoma technology and monoclonal
antibodies, immunoblotting, flow cytometry. Single Radial Immuno Diffusion Double diffusion.Rocket
Immunoelectrophoresis.
Text/Reference Books:
1.Essential Immunology, Eleventh Edition (2006) P. Delves, S. Martin, D. Burton and I. Roitt,
Wiley-Blackwell Publishers, UK.
2. Immunology, Third Edition (1997) J. Kuby, W H Freeman & Co Publishers.
3. Practical immunology, Fourth Edition (2002) F.C. Hay and O.M.R. Westwood, WileyBlackwell
Publishers.
MTB 203: Bioinformatics
L T P Credit: 4
3 1 0
UNIT I
Biological Databases:Format & Applications of Sequence and Structure databases of Nucleic Acids &
Proteins (GENBANK, EMBL, DDBJ, PIR, UNIPROT, PDB, SCOP, CATH, PRINT, Pfam etc) Specialized
databases.
UNIT II
Sequence Analysis: Introduction to DNA and protein sequence analysis, Translation, ORF finding,
BLAST, FASTA Local vs Global alignment, Pair wise Sequence Analysis, Multiple sequence Analysis,
Scoring matrices, Analysis, Web based tools for sequence analysis, Dynamic Programming, Phylogenic
trees
UNIT III
Functional, structural and comparative genomics, protein structure, Homology modeling, gene expression,
Electronic PCR, Primer designing, Meth Primer Designing.
UNIT IV
Hidden Markov models, neural networks, and their application in computation biology, Eukaryotic and
prokaryotic gene finding, DNA computing.
UNIT V
Chemoinformatics, Pharmacogenomics and Pharmacogenetics, practicals on data retrieval involving
internet based Bioinformatics tools. Protein Function Prediction, Metabolic Pathway analysis,
Computer aided drug designing and computational vaccine desigining,
Text/Reference Books:
1. David Edwards, Jason Eric Stajich, David Hansen, “Bioinformatics: Tools and Applications” , Springer,
2009.
2. David W Mount, “Bioinformatics: Sequence and genome analysis”, Cold spring harbor laboratory press,
2nd
edition, 2004.
3. Attwood T K, D J Parry-Smith, “Introduction to Bioinformatics” ,Pearson Education, 2005.
4. ParagRastogi, “Bioinformatics Methods and Applications: Genomics Proteomics And Drug Discovery”,
PHI Learning Pvt. Ltd., 3 rd
edition, 2008
5. Tisdall,James, “Beginning Perl for Bioinformatics”, O, Reilley,2001.
6. Baxevanis A.D and Oullette, B.F.F., “A Practical Guide to the Analysis of Genes and Proteins”, 2nd
Edition , John Wiley,2002.
MTB 204: Advanced Environmental Biotechnology
L T P Credit: 4
3 1 0
UNIT I Environmental components, Natural resources, Ecosystem and its diversity, Environmental pollution and its
major impacts, Global warming and greenhouse effect, Global Ozone Problem, Acid rain, Eutrophication,
Land degradation, Biomagnification
UNIT II Non-renewable and renewable energy resources, concept of clean fuel technology, Biomass energy and
biofuels
Unit III
Biodegradation and bioremediation of major pollutants, Biomineralisation: Use of microbial technology for
mining
UNIT IV Waste water engineering: Treatment of municipal wastes and industrial effluents with special focus on use
of biological methods, Advanced waste water treatments
UNIT V Bioassessment of environmental quality: Biosensors and biomarkers, Biofertilizers, Biopesticides and
Vermi composting. Environmental impact assessment and Environmental audit, Related case studies from
Indian market
Text/Reference Books:
Environmental Science, S.C. Santra
Environmental Biotechnology, Pradipta Kumar Mohapatra
Environmental Biotechnology – Concepts and Applications” by Hans-Joachim Jordening and Jesef
Winter
Waste Water Engineering by Metcalf and Eddy. Publisher: Tata McGraw hill
Environmental Microbiology: Methods and Protocols by Alicia L. Ragout De Spencer, John F.T.
Spencer
Introduction to Environmental Biotechnology by Milton Wainwright
Principles of Environmental Engineering by Gilbert Masters
MTB 205: Plant Biotechnology
L T P Credit: 4
3 1 0
UNIT I
Introduction to plant biotechnology, Objective, Roles and landmarks in plant breeding. Introduction to the
techniques of plant tissue culture. Concept of cellular totipotency, Nutritional requirements, single cell
culture, micro-propagation, Somaclonal variation, somatic embryogenesis and production of embryoids.
UNIT II
Haploid and double haploid production, Protoplast isolation and culture. Somatic hybridization and cybrid
production and their applications in crop improvement. Productions of virus free plants using meristem
culture.
UNIT III
Basis of tumor formation, hairy roots, features of Ti and Ri plasmids, mechanisms of DNA transfer, role of
virulence genes, use of Ti and Ri as vectors, binary vectors, use of 35S and other promoters, genetic
markers, use of reporter genes, methods of nuclear transfer, particle bombardment, electroporation,
microinjection, transformation of monocots. Transgene stability and gene silencing. Herbicide and
resistance. insect Plant Genetic Engineering: Transgenic plants, Genetically modified (GM) plants (Bt
cotton, Bt Brinjal)
UNIT IV
Photoregulation and phytochrome regulation of nuclear and chloroplast genes expression, Molecular
biology of light and dark reactions of photosynthesis, Genetics of nif genes, Molecular mechanism of
nitrogen fixation.
UNIT V
Plant secondary metabolites: Control mechanisms and manipulation of alkaloids and industrial enzymes
(Shikimate and PHA pathway), biodegradable plastics, therapeutic proteins, Edible vaccines, purification
strategies. Green house Technology. Biotic and Abiotic stress.
Text/Reference Books:
1.Plant Tissue Culture by MK Razdan & SS Bhojwani (1996) Elsevier
2. Plant Physiology by L Taiz & E Zeiger 4th Edition (2006) Sinauer Associates Inc, Publishers
3. Experiment in Microbiology, Plant pathology and Tissue culture by K.R. Aneja, Wishwa Prakashan
4. Genetic Transformation of Plants, Edited by Jackson, J.F.; Linskens, H.F. , Springer 2003
5. Plant Biotechnology and Transgenic Plants, Edited by Kirsi Marja Oksman-Caldentey, Wolfgang Barz Marcel Dekker 2002
6. Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, Robert N Trigiano, Dennis J Gray, CRC Press
November 1999
MTB 206: Food Biotechnology
L T P Credit: 4
3 1 0
UNIT I
Food chemistry: Constituent of food – contribution to texture, flavour and organoleptic properties of food;
food additives – intentional and non-intentional and their functions; enzymes in food processing.
UNIT II
Food microbiology: Sources and activity of microorganisms associated with food; food fermentation; food
chemicals; food borne diseases – infections and intoxications, food spoilage – causes.
UNIT III
Food processing: Raw material characteristics; cleaning, sorting and grading of foods; physical conversion
operations – mixing, emulsification, extraction, filtration, centrifugation, membrane separation,
crystallization, heat processing.
UNIT IV
Food preservation: Use of high temperatures – sterilization, pasteurization, blanching, asceptic canning;
frozen storage – freezing curve characteristics. Factors affecting quality of frozen foods; irradiation
preservation of foods.
UNIT V
Manufacture of food products: Bread and baked goods, diary products – milk processing, cheese, butter,
ice-cream, vegetable and fruit products; edible oils and fats; meat, poultry and fish products; confectionery,
beverages.
Text/Reference Books:
1. Coultate T.P. Food – The chemistry of its components, 2
nd ed., Royal society,
London, 1992
2. Sivasankar B. Food processing and preservation, Prentice Hall of India Pvt.Ltd., New
Delhi, 2002
3. Fennema O.R. ed. Principles of food science : Part I, Food chemistry, Marcel
Dekker, New York, 1976.
4. Frazier W.C. and Westhoff D.C. Food Microbiology, 4th
ed. McGram-Hill Book Co.,
New York, 1988
5. Brenner, J.G., Butters, J.R., Cowell, N.D. and Lilly, A.E.V. Food engineering
operations, 2nd
ed., Applied Sciences Pub.ltd., London,1979
6. Pyke, M. Food Science and Technology , 4th
ed., John Murray, London, 1981
MTB 207: Nanobiotechnology
L T P Credit: 4
3 1 0
UNIT I
Introduction to nanotechnology and Interphase systems: Principles in nanotube formation, characteristics of
nanoparticles, particle agglomeration, carbon nanotubes, nanostructured polymers.
UNIT II Biocompatible inorganic devices, Lab-on-a-chip devices, microcontact printing of proteins, cell
nanostructure interactions, defined networks of neuronal cells in vitro, quantum dots, DNA microarrays
.
UNIT III
DNA-based nanostructures: DNA-Protein nanostructure, DNA-templated electronics, biomimetic
fabrication of DNA-based metallic nano-wires and networks, DNA-gold particle conjugates.
UNIT IV
NanoMedicens : Developing of Nanomedicens Nanosytems in use, Protocols for nanodrug Administration,
Nanotechnology in Diagnostics applications, materials for used in Diagnostics and Therapeutic applications.
UNIT V
Application of nanobioparticles : Bacteriorhodopsin and its potential in technical applications, Nanoscale
biosensors.
Text/Reference Books:
Nanobiotechnology: Concepts, applications and perspectives, eds. CM Niemeyer, CA Mirkin,
Wiley-VCH Verlag GmbH & Co., KgaA, Weiheim (2004).
Nanotechnology: An introduction to nanostructuring techniques, eds. M Kohler, W Fritzsche,
Wiley-VCH Verlag GmbH & Co., KgaA, Weiheim.
Handbook of nanotechnology, ed. Bhusan, Springer-Verlag, Berlin, Heidelberg, Germany.
Nanocomposite science and technology, eds. PM Ajayan, LS Schadler, PV Braun, Wiley-VCH
Verlag, GmbH & Co., KgaA, Weiheim
MTB 251: Molecular Biology & Immunology Lab
L T P Credit: 2
0 0 3
MOLECULAR BIOLOGY LAB
1. Isolation of bacterial DNA
2. Isolation of Plasmid DNA.
3. Isolation of plant DNA.
4. Agarose gel electrophoresis.
5. Estimation of DNA content in the given sample by diphenylamine method.
6. Estimation of RNA content by the Orcinol method.
IMMUNOLOGY LAB
1. To determine the blood group of given blood
2. To determine the Rh factor of given blood
3. To perform single radial immunodiffusion
4. To perform double immunodiffusion
5. To perform rocket immune electrophoresis
6. To perform counter current immune electrophoresis
7. To perform Sand-witch ELISA
8. To perform Indirect ELISA
9. To perform Competitive ELISA
10. To prepare the blood smear and stain with leishman stain
11. To identify the blood cells/ immune cell with the help of leishman stain
12. To perform differential count (DLC) of given sample
Reference books
1. Handbook of Experimental Immunology, Vol. I & II, IV- Blackwell Scientific Publications.
2. Practical Immunology- Hudson L. and Hay H. C. Blackwell Scientific Publications.
3. Hybridoma Techniques: A Lab Course- Muthukkaruppan Vr, Basker S and F. Singilia.
Macmillan India Ltd.
MTB 252: Bioinformatics Lab
L T P Credit: 2
0 0 3
LIST OF EXPERIMENTS:
1. Bioinformatics databases
2. Pair wise sequence alignment
3. Sequence similarity searching for sequences
4. Multiple sequence alignment and editing
5. Phylogenetic analysis using distance based methods & character based methods
6. Evaluation of trees
7. Gene prediction tools
8. Prediction Of secondary Structure of proteins
9. Sequence based prediction and Validation of 3DProtein structure 10. Docking studies
MTB 301: Advanced Genetic Engineering
L T P Credit: 4
3 1 0 UNIT I
Restriction endonucleases: Types of restriction endonucleases, classification and uses. Restriction mapping.
DNA modifying enzymes: Nucleases, Polymerases, Phosphatases and DNA ligases. Joining of DNA
Fragments to vectors, Homo polymer tailing, cohesive and blunt end ligation, adaptors, and linkers.
UNIT II
Prokaryotic host. Plasmid vectors, phage vector, cosmid, phagemid, BAC,YAC & other vectors, Expression
vectors, Plant vector (Ti- plasmid). Construction of genomic and c-DNA libraries,
UNIT III
Polymerase chain reaction (PCR), variant of PCR and its applications.Cloning, Selection, screening and
analysis of recombinants. Principle of hybridization, Northern blotting, Southern blotting, Western blotting.
Polymerase chain reaction, Site directed mutagenesis. Restriction fragments length polymorphism, RAPD,
AFLP. Genetic marker, Protein-protein interaction.
UNIT IV
Isolation of DNA and RNA. Quantification of nucleic acids. Radiolabelling of nucleic acids: End labelling,
nick translation, labelling by primer extension, DNA sequencing: Maxam-Gilbert (Chemical) and Sanger-
Nicolson (dideoxy/ enzymatic) sequencing method, Pyrosequencing.
UNIT V
Vector Engineering and codon optimization, host engineering. Strategies of gene delivery, in vitro
translation, expression in bacteria and yeast, expression in insects and insect cells, expression in mammalian
cells, expression in plants. Chromosome engineering, Targeted gene replacement, gene editing, gene
regulation and silencing.
Text/Reference Books:
1. Principles of Gene manipulation(1994) Old R.N. and Primrose S.B.
2. From Genes to Clones (1987) Winnaeker E.L.
3. Recombinant DNA (1992) Watson J.D., Witreowski J., Gilman M. and Zooller M.
4. An Introduction to GeNETIC Engineering: Nicholl, D.S.T.
5. Molecular Biotechnology (1996) Pasternak
6. The Biochemistry of Nucleic acid(1996)Adam et al
7. Genetic Engineering (1998)Janke k. swtlow
MTB 302: Bioreactor Engineering
L T P Credit: 4
3 1 0
UNIT I Bioreactors: Modes of bioreactor operations,components and their functions. Bioreactor design: Batch
reactor, cell death in batch reactor, chemostat, maintenance, product & substrate inhibition on chemostat,
multiple steady state analysis, plug flow reactor, fed batch reactor, Chemostat with cell recycle.
UNIT II
Mass transfer I: molecular diffusional theory, Convective mass transfer, Factors affecting kLa, Interfacial
area and oxygen transfer, Factors affecting the saturation concentration of oxygen, Oxygen uptake. Gas-
liquid mass transfer in cellular systems, solubility of gases (O2, CO2) in biological media, Mass balance for
two-phase bioreactor.
UNIT III
Mass transfer II: Bubble column, bubble generation at an orifice, bubble coalescence and reakup, gas
holdup, interfacial area, immobile and mobile gas liquid interface, Regimes of bubbles, Design of bubble
columns.Mass transfer in agitated tanks, correlation with kLa in Newtonian and nonNewtonian fluid, power
number.determination of kLa, Power requirement for mixing, agitated and non-agitated tanks for Newtonian
and non-Newtonian liquid. Mixing time in agitated rector, Residence time distribution, non ideal reactor and
multiphase bioreactor. UNIT IV
The Stirred Tank Bioreactor: Introduction, Standard geometry of stirred tank bioreactor, Basic features of
stirred tank bioreactor; agitation systems, oxygen delivery, foam control, temperature control systems, pH
control systems, cleaning and sterilization facilities Agitator Design and Operation: flow patterns,
preventing vortex formation, off centre impellers, baffles. Type of impellers, Power number, Power input,
Mixing, Gas holdup, Sheer stress calculation. UNIT V
Aeration And Agitation In Animal Cell Bioreactors: Introduction, cell damage in animal cell bioreactor,
shear damage, bubble damage, Methods of minimizing cell damage Scale Up of Bioreactor, Control of
Bioreactor: Sensor used in the bioreactor. Online sensors for cell properties, online and offline monitoring
of bioreactor; feed back control, cascade control and computerized bioprocess control.
Text/Reference Books:
1. Blanch HW and Clark DS: Biochemical Engineering Marcel Decker Year of Publication.
2. Tapobrata panda: Bioreactors Analysis and Design: Year of Publication 2011:Tata Mcgraw Hill
publication
MTB 303: Genomics & Proteomics
L T P Credit: 4
3 1 0 UNIT I
Overview- Structure and organization of Prokaryotic and eukaryotic genome, gene structure and gene
density of prokaryotes and eukaryotes, variations in the general structure and organization in genomes
expression profiles.
UNIT II
Brief outlook of various genome projects and their outcome, Nucleotide and protein sequencing, Genome
sequencing; various techniques: shot gun, clone contig approach, chromosome walking, primer walking,
chromosome jumping, contig assembly, genome marking and mapping techniques, genomic DNA-
library, cDNA-library.
UNIT III
Mapping and Sequence Assembly, Genetic markers-RFLP, STR, SSLP, VNTR. Physical markers, ESTs,
STS, FISH, Radiation hybrid, Sequence marker, SNPs, Expression analysis, Obtaining & Evaluating gene
expression profiles with mircoarray.
UNIT IV
Human genome project - transcript on structural and functional genomics, comparative and population
genomics, Pharmacogenomics and Phylogenetics, Functional Proteomics, genotype-phenotype relationship,
polygenic nature of proteins
UNIT V
Proteomics experimental techniques: 2-D electrophoresis, Mass spectrometry & Protein Microarray,
MALDI-TOF ,Drug Design, Empirical Methods and prediction techniques, X-ray and NMR-structures,
Post-translational modification prediction: protein sorting, proteolyticcleavage, glycosylation and
phosphorylation.
.
Text/Reference Books:
1. Introduction to Genomics . Arthur Lesk. Oxford University Press, 2008
2.Brown TA, Genomes, 3rd Edition, Garland Science, 2006.
3. Campbell AM & Heyer LJ, Discovering Genomics, Proteomics and
MTB 304: Animal Biotechnology
L T P Credit: 4
3 1 0 UNIT I
Brief history of animal cell and organ culture, cell culture media, immobilized cell culture, insect cell
culture, somatic cell culture.
UNIT II
Cell lines cultivation (monolayer's, suspension culture, stationary suspension culture agar culture and
agitated microcarrier suspension culture, hollow fiber systems)
UNIT III
Cell synchronization and cell transformation. Maintenance of cell culture through sub- culturing and
cloning, cryopreservation.
UNIT IV
Specific applications of cells in culture; Pharmaceuticals, vaccines, monoclonal antibodies, recombinant
proteins, Stem cell technology
UNIT V
Embryonic culture, gene transfers and transgenic animals and embryo transfer technology, Tissue
engineering
Text/Reference Books:
Cell culture ,Spring Harbour Lab, New York, Animal Cell Culture Course Manual.
Fresnay, P.E.& Patterson M.K., Tissue Culture Methods & Application.
Molecular Biotechnology: Principles and Applications of recombinant DNA 2nd
Edition. Glick, B.
R. and Pasternak J. J. (1998) ASM press, Washington DC.
Animal Cell biotechnology : Spier, R.E. and Griffiths J.B. (1988) Academic press.
Living resources for Biotechnology, Animal cells. Doyle, A.; Hay, R. and Kirsop, B.E. (1990)
Cambridge University Press, Cambridge.
Animal Biotechnology. Murray Moo-Young (1989) Pergamon Press, Oxford.
MTB 305: Pharmaceutical Biotechnology
L T P Credit: 4
3 1 0 UNIT I
DNA, RNA, Protein modifications/events, post-translational, processing enzymes, G protein coupled
receptors (monomeric transmembrane proteins), small molecule receptors, neuropeptide receptors, ion
channels (monomeric multi-transmembrane) proteins, ligand-gated ion channels (oligomeric transmembrane
proteins), transporters (multi-transmembrane proteins).
UNIT II
Introduction to drug discovery and development, target discovery and validation strategies: Genomics (new
target discovery), biological activity directed and other types of screening, natural products, combinatorial
chemistry. Pharmacakinetics and Tonicological considerasation.
UNIT III
Computer aided drug design, Structure-based design: ‘de novo’ design methodologies: docking.
UNIT IV
Design and development of combinatorial libraries for new lead generation: The molecular diversity
problem, drug characterization – principles of equilibria, diffusion and kinetics, preformulation: pKa,
partition coefficient, solubility, dissolution, chemical stability, and permeability, optimization of ADME
characteristics, physico-chemical properties calculation, Linear Free energy, Hanseh equation, Hammett
euation, chemiometrics in drug design.
UNIT V
Statistical techniques behind QSAR, classical QSAR, molecular descriptors 3D QSAR and COMFA, drug
design to discovery and development
Text/Reference Books:
Introduction to Biophysical Methods for Protein and Nucleic Acid Research by J.A. Glasel and M.P.
Deutscher, Academic Press
Principles of Drug Action” by W.B. Pratt and P. Taylor, Churchill Livingston.
Principles of Medicinal Chemistry” by W.O. Foye, T.L. Lemke, and D.A. Williams, Williams and Wilkins.
Side Effects and Drug Desig by E.J. Lien, Marcel Dekker
The Anticancer Drugs by W.B. Pratt, R.W. Ruddon, W.D. Ensminger, and J. Maybaum, Oxford University Press.
MTB 306: IPR, Bioethics and Biosafety
L T P Credit: 4
3 1 0 UNIT I
General Overview of Intellectual Property Rights, WIPO, WTO, Trade Related Intellectual Property Rights.
UNIT II
Patent - Basic requirements of Patentability, Patentable Subject Matter, Procedure for Obtaining Patent,
Provisional and Complete Specification
UNIT III
Copyright - Objectives of copyright, Rights conferred by registration of copyright, Infringement of
copyright
UNIT IV
Trademarks-Basic Principles of Trademark, Rights conferred by Registration of Trademark, Infringement of
Trademark
UNIT V
Geographical Indications-Objectives of Geographical Indications, Rights conferred, Infringement of
Geographical Indications, International Position, Indian Position, Bioprospecting and Biopiracy.
UNIT VI
Biosafety and Bioethics Management-Key to environmentally responsible use of biotechnology. Cartagena
Protocol on Biosafety, Ethical implications of Biotechnological products and techniques.
Text/Reference Books:
Intellectual Property Rights by Brigitte Anderson, Edward Elgar Publishing
Intellectual Property Rights and the Life Sciences Industries by Graham Dutfield, Ashgate Publishing
WIPO Intellectual Property Handbook
Intellectual Property Rights by William Rodelph Cornish, David Clewelyn
Journals and Current magazines
MTB 307: Research methodology in Biotechnology
L T P Credit: 4
3 1 0
UNIT I Research and its methodologies: Objectives of research, research process-observation, analysis, inference, hypothesis,
axiom, theory, experimentation, types of research – basic, applied, quantitative, qualitative, analytical etc. features of
translational research, concept of laboratory to market and industrial R & D.,
UNIT II Research in biotechnology- an overview: Biological systems and their characteristics that influence the type and
outcome of research, exploratory and product oriented research in various field of biotechnology- health, agri, food,
industrial etc. interdisciplinary nature of biotech research, sources of literature for biotech research.
UNIT III Computer applications in Biology: Spreadsheet tools : Introduction to spreadsheet applications, features, Using
formulas and functions, Data storing, Features for Statistical data analysis, Generating charts / graph and other
features, Tools – Microsoft Excel or similar. Presentation tools: Introduction, features and functions, Presentation of
Power Point Presentation, Customizing presentation, Showing presentation Tools – Microsoft Power Point or Similar.
Web Search: Introduction to Internet, Use of Internet and WWW, Use of search engines, Biological data bases.
UNIT IV Results and analysis:Importance and scientific methodology in recording results, importance of negative results,
different ways of recording, industrial requirement, artificial versus true results, correlation with published results,
discussion, outcome as new idea, hypothesis, concept , theory , model.
UNIT V Scientific and technical publication:Different types of scientific and technical publications in the area of
biotechnology, ways to protect intellectual property-patents, technical writing skills, impact factor and citation index.
Text/Reference Books:
Essentials of research design and methodology Geoffrey R. Marczyk, David DeMatteo, David Festinger,
2005 Jhon Wiley & Sons publishers,Inc.
Guide to publishing a scientific paper, Ann M. Korner, 2004, Bioscript Press.
Introductory Statistics. Fifth Edition. (2004) Prem S. Mann. John Wiley and Sons (ASIA) Pvt. Ltd.
MTB 308: Enzyme Technology & Industrial Applications
L T P Credit: 4
3 1 0
UNIT I
Extraction and Purification of Crude Enzyme extracts from plant, animal and microbial sources-some case
studies; methods of characterization of enzymes; development of enzymatic assays.
UNIT II
Mechanisms of Enzyme Action; Concept of active site and energetics of enzyme substrate complex
formation; Specificity of enzyme action; Kinetics of single substrate reactions; turnover number; estimation
of Michaelis-Menton parameters. Importance of Km, Multi-substrate reaction mechanisms and kinetics.
UNIT III
Types of Inhibition- kinetic models; Substrate and Product Inhibition; Allosteric regulation of enzymes;
Deactivation kinetics.
UNIT IV
Physical and Chemical techniques for enzyme Immobilization – adsorption, matrix entrapment,
encapsulation, cross-linking, covalent binding - examples; Advantages and disadvantages of different
Immobilization techniques; Overview of applications of immobilized enzyme systems, Applications of
enzymes in analysis; Design of enzyme electrodes and their applications as biosensors in industry, health
care and environment.
UNIT V
Design of Immobilized Enzyme Reactors-Packed- bed, Fluidized-bed Membrane reactors; Bioconversion
calculations in free- enzyme CSTRs and immobilized enzyme reactors.
Text books:
Fundamentals of enzymology by Nicolas C. price and Lewis stevens . Oxford University Press
Enzymes by Trevor palmer, East west Press
nzyme Technology by Messing
Reference books:
Enzymes : Dixon and Webb.(IRL Press)
Enzyme technology by Chaplin and Bucke. Cambridge Univerity Press
Biochemical engineering fundamentals, second edition. James E Bailey, David F., Ollis, McGraw Hill Intl. Edition
MBT-451: DISSERTATION
A student has to make a latest technology based project in their respective stream. It may be
hardware or software based.
Convener
Signature: ………………………
Name : Dr. Ajay Kumar
Date :
Internal Members
Signature: 1…………………………………. 2………………………………………
Name: Mr. Ajit Pratap Singh Yadav Mr. Vachaspati Rao
Date:
External Members
Signature: 1……………………………………… 2…………………………………………
Name: Prof. (Dr.). Dr.
Date: