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LANXESS Deutschland GmbH, Paul Marquardt
Vienna, 08.09.2014
Microbiological stabilization of Beer-Mix beverages
with Dimethyl-Dicarbonate – first practical experiences
1 Business Unit Material Protection Products
DMDC – Genie in a bottle
Product Launches
DMDC Technology
Microbiology & Sensory
Registration
Application
2 Title of presentation ▪ Speaker ▪ Place, date … [ OPTIONAL ]
Beer-Mix beverages is a very innovative product category
60
84
15
4 4 Europe
NorthAmerica
Middle East& Africa
Asia Pacific
Product introduction by Regions 2013* Global product launches by flavour, 2009-13*
* Mintel , Beer Mixes, Feb 2014
29%
24% 6% 4%
4% 3%
30%
Lemon
Lemonade
Grapefruit
Cola
Apple
Lime
Others Product launches by packing*
60% 40%
3 Business Unit Material Protection Products
DMDC – Genie in a bottle
Product Launches
DMDC Technology
Microbiology & Sensory
Registration
Application
4 Business Unit Material Protection Products
DMDC has a unique operating principle
lipophilic
cell membrane
[pH : 2 - 4 (acidic)] [pH : 7 (neutral)]
inactivated enzyme with
carbo-methoxylated imidazole group
penetration
effect
on
250 mg dimethyl-dicarbonate
water
120 mg
methanol
natural occurring
substances 162 mg
carbon-dioxide
+
H3C O O O
O
CH3
O
O 2
+ H3C
OH 2 CO2 2
H
active enzyme with
Imidazole group
Beer Mix Beverage Cell interior
N
N
R2
H
R1
H
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DMDC hydrolyses rapidly, especially in warmer environments
0 %
20 %
40 %
60 %
80 %
100 %
0' 30' 60' 90' 120' 150' 180' 210' 240'
Time (Minutes)
DM
DC
20 °C / 68°F
10 °C / 50°F
4 °C / 39°F
Hydrolysis: DMDC + Water = Methanol + CO2
6 Business Unit Material Protection Products
DMDC – Genie in a bottle
Product Launches
DMDC Technology
Microbiology & Sensory
Registration
Application
7 Business Unit Material Protection Products
DMDC needs to be dosed inline to the finished Beer-Mix beverage with specialized dosing equipment
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De- centralization of production to sites without tunnel-pasteurizers
Flexible use for heat-sensitive packaging
Lower energy consumption
Reduction of foot print
Replacement of tunnel-pasteurizer
Improve taste
DMDC targets relevant micro- spectrum yeasts
Clean label in many countries
Remove Persistent preservatives
Bottling more sensitive beer-mix beverages
Switch from sugar-free to “full” version beer mix beverages
Highly flexible application
Low investment
Utilize existing beer filling machines
Beer-Mix beverages – opportunities with DMDC
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Beer-Mix beverages – first experiences with DMDC
First Customer Projects:
Discussion about Beer-Mix beverages in heat-sensitive packing for export
Trials in Wheat Beer-Mix beverage from sugar-free to “full” version
Trials with “Radler” on existing bottling lines
Running business:
Customers in Germany with Velcorin® in Beer-Mix beverages on existing
bottling lines in order to secure microbiological quality.
Velcorin® for Beer-Mix beverages
10 Business Unit Material Protection Products
DMDC – Genie in a bottle
Product Launches
DMDC Technology
Microbiology & Sensory
Registration
Application
11 Business Unit Material Protection Products
Internal microbiological challenge test – microorganisms
Beer Mix
Lager Beer + Lemon soda
pH = 3,4
Microorganisms
Pichia anomala
Candida tropicalis
Saccharomyces cerevisiae
Zygosaccharomyces bailii Bier Mix
Wheat Beer + Lemon
soda
pH = 3,4
Matrix
Lactobacillus brevis
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Internal microbiological challenge test-parameters
Parameter
Beer Mix
Lager Beer + Lemon soda
pH = 3,4
Temperature
Germ loading
DMDC concentration
Value
5-10 °C
Target: 30 CFU/ml and 300 CFU/ml
0 ppm and 250 ppm
Replication Duplicates
Incubation 18 – 20 °C for 7 days
Bier Mix
Wheat Beer + Lemon
soda
pH = 3,4
Matrix
Nutrient Media MRS Agar & Malt Extract Agar
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Internal microbiological challenge test-results
Matrix
Germ Loading
Start 1 Week Start 1 Week Start 1 Week Start 1 Week
Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL
54/49 20/14 640/540 +/+ 20/28 3/- 568/444 +/+
31/38 12/10 804/640 +/+ 26/25 -/- 616/520 +/+
48/41 -/- 536/616 -/- 22/20 -/- 604/570 -/-
32/45 -/- 632/740 -/1 19/31 -/- 536/636 -/-
Beer Mix - Lager Beer Mix - Wheat
0 ppm DMDC
250 ppm DMDC
I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml II 300 CFU/ml
Pichia anomala
Candida tropicalis
Matrix
Germ Loading
Start 1 Week Start 1 Week Start 1 Week Start 1 Week
Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL
1/1 -/- 84/76 4/6 -/- -/- 152/125 1/3
-/3 -/- 123/108 -/- -/- -/- 138/126 77/45
2/1 -/- 68/71 -/- -/- -/- 133/148 -/-
1/3 -/- 126/93 -/- -/- -/- 127/165 -/1
II 300 CFU/ml
0 ppm DMDC
250 ppm DMDC
Beer Mix - Lager Beer Mix - Wheat
I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml
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Saccharomyces cerevisiae
Zygosaccharomyces bailii
Matrix
Germ Loading
Start 1 Week Start 1 Week Start 1 Week Start 1 Week
Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL
28/26 +/+ 324/252 +/+ 29/27 90/98 298/314 +/+
34/25 +/+ 312/308 +/+ 24/27 24/25 294/342 +/+
24/22 -/- 260/256 -/- 27/27 -/- 340/266 -/-
30/30 -/- 300/308 -/- 34/20 -/- 258/234 -/-
II 300 CFU/ml
0 ppm DMDC
250 ppm DMDC
Beer Mix - Lager Beer Mix - Wheat
I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml
Matrix
Germ Loading
Start 1 Week Start 1 Week Start 1 Week Start 1 Week
Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL
23/27 1/- 274/240 16/23 30/13 1/2 352/280 3/1
30/18 -/- 260/273 5/5 20/20 5/9 384/280 24/21
20/14 -/- 276/215 -/- 24/28 -/- 262/206 -/-
25/22 -/- 238/232 -/- 18/20 -/- 340/240 -/-
Beer Mix - Lager Beer Mix - Wheat
0 ppm DMDC
250 ppm DMDC
I 30 CFU/ml II 300 CFU/ml I 30 CFU/ml II 300 CFU/ml
Internal microbiological challenge test-results
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No growth of Lactobacillus brevis with / without Velcorin® treatment. Microbe obviously
not adapted to the matrix
Lactobacillus brevis
Matrix
Germ Loading
Start 1 Week Start 1 Week Start 1 Week Start 1 Week
Matrix Amount 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL 1 mL 10 mL
4/1 -/- 85/67 -/- 2/2 -/- 94/77 -/-
-/- -/- 69/71 -/- 1/- -/- 77/74 -/-
2/- -/- 65/96 -/- 2/1 -/- 90/51 -/-
1/7 -/- 77/64 -/- 2/- -/- 84762 -/-
I 30 CFU/ml II 300 CFU/ml
0 ppm DMDC
250 ppm DMDC
I 30 CFU/ml II 300 CFU/ml
Beer Mix - Lager Beer Mix - Wheat
Internal microbiological challenge test-results
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Sensory triangle test – no influence on taste
Lager type + lemon soda
Beer Mix
Lager type + lemon juice
Dealcoholized Lager type
+ lemon soda
Wheat beer + lemon soda
Lager type + energy drink
Triangle test according MEBAK II 2.34.7
No statistical relevant difference
No statistical relevant difference
No statistical relevant difference
No statistical relevant difference
No statistical relevant difference
Test performed by
Forschungszentrum
Weihenstephan August 2014:
Beverage treated with DMDC
against
beverage without DMDC
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Further test at Forschungszentrum Weihenstephan für Brau- und Lebensmittelqualität
Lager type + lemon soda
Microorganisms to be tested
Lactobacillus brevis-FZ BLQ 4
Pectinatus frisingensis-FZ BLQ HB S1
Saccharomyces cerevisiae-FZ BLQ H TUM 68
Saccharomyces pastorianus ssp. Carlsbergensis
-FZ BLQ H TUM 34/70
Beer Mix
Saccharomyces cerevisiae var. Diastaticus
-FZ BLQ TUM SY 1
Lager type + lemon juice
Dealcoholized Lager type
+ lemon soda
Wheat beer + lemon soda
Lager type + energy drink
Dekkera anomala-FZ BLQ 2-C-1
Wickerhamomyces anomalus FZ BLQ 17-C-3
Kazachstania exigua-FZ BLQ H2-G-7
pH
Alcohol [Vol %]
BU
CO2 [GV-%]
F.S. [g/100ml]
Analytical parameters
18 Business Unit Material Protection Products
DMDC – Genie in a bottle
Product Launches
DMDC Technology
Microbiology & Sensory
Registration
Application
19 Business Unit Material Protection Products
BMB with Lemon Soda and/ or Juice
BMB with Cola, energy drink, etc.
Alcohol free BMB
Sweetened / Unsweetened BMB
Flavoured Beer
Beer with Spirits
Malt Beer
Beer-Mix Beverages Categorization
Regulatory Categorisation of Beer Mix Beverages
Beverage Category Regulation (EC) No. 1333/2008, Annex II: Category No.: 14.2.8
Other alcoholic drinks including mixtures of alcoholic drinks with non-alcoholic drinks and spirits with less
than15% of alcohol
No registration of DMDC for Beer and Malt beverages (Category 14.2.1)
Labelling: Regulation (EC) No. 1169/ 2011, Article 16(4):
4. Without prejudice to other Union provisions requiring a list of ingredients or a mandatory nutrition declaration, the
particulars referred to in points (b) and (l) of Article 9(1) shall not be mandatory for beverages containing more than 1,2 % by
volume of alcohol. Therefore there is no obligation to label DMDC
Outside Focus Beer Beverages Focused Beer-Mix Beverages
20 Business Unit Material Protection Products
Not present in final beverage
No declaration on ingredients list
No allergen or GMO
No effect on colour or taste
Compatible with all packaging
Extensive technical Service
Benefits and other applications of DMDC
Why Velcorin® ? Use of Velcorin in other Beverage categories
Carbonated & Still Juice Beverages Isotonic Sports Beverages
Ice Teas
Flavoured Waters Wines
Ciders
22 Business Unit Material Protection Products
Disclaimer
-Presentation is no hand out, for presentation purposes and internal use only! -
This information and our technical advice - whether verbal, in writing or by way of trials - are given in good
faith but without warranty, and this also applies where proprietary rights of third parties are involved. Our
advice does not release you from the obligation to verify the information currently provided - especially that
contained in our safety data and technical information sheets - and to test our products as to their suitability
for the intended processes and uses. The application, use and processing of our products and the products
manufactured by you on the basis of our technical advice are beyond our control and, therefore, entirely
your own responsibility. Our products are sold and our advisory service is given in accordance with the
current version of our General Conditions of Sale and Delivery.
The handling of Velcorin® , as a chemical substance, is subject to the German Hazardous Substance
Ordinace and the corresponding EC Directive. The following hazard characteristics should be noted:
harmful (if swallowed), toxic (by inhalation), corrosive.
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Due to Velcorin‘s properties, it can be regarded as a processing aid without requirement for labelling
Codex Alimentarius General Standard for Food Additives (Categories 14.1.4 and 14.1.5)
Inventory list of substances used as processing aids
EU (EC) No 1333/2008
USA Food Additive Provision and Food Contact Notification
Halal & Kosher
No Labelling Labelling
Germany
USA
Mexico
Russia
Australia/New Zealand
…
Turkey
Brazil
India
China
South Africa
…
References
Processing aid function Preservative function
Max. dosage in non-alcoholic soft-drinks: 250ppm
24 Business Unit Material Protection Products
Velcorin also complies with ethnic and religous dietary laws. Certificates are available
Halal
Kosher
Issued by Halal Control Europe
Accredited & appointed by e.g.
-JAKIM (Department of Islamic Development Malaysia)
-LP-POM MUI Indonesia (Indonesian Council of Ulama)
Certificates available from
- Orthodox Union (OU)
- Kosher Supervision