Post on 27-Apr-2020
transcript
ø23.1
Focal point
30.7
100.
1
27.3
4
17.5
ø53ø42
(Min
. ang
le)
142
582
.8
51.9 85.6
ø51.5
10˚
2112
5.8
ø60
11.3
40.3
151.
3
4.5
46.5
99.8
ø29.
1
ø30
10.5
ø54
48
ø116
ø43.5
80.7
117.8
53
31.8
54.8
7.7
ø40
70˚
M25×0.75ø32
ø10.5ø22
ø27.5ø32.5ø34
30˚
C0.5
1.2(
CG
)
60.4
1
12.8
519
.55
24.7
1.8
(5.1
1)
3.1(
WD
)
5˚
5.2
C0.5
C0.5
7˚
539
.515
.9
10.9
40X /0.60LWDS Plan Fluor
DIC N1
0.171.22 0
Microscope Components for FluorescenceIllumination and Transmitted Light Applications
Microscope Components for Fluorescence Illumination and Transmitted Light Applications
2
Nikon's biological microscope units, including objective lenses and eyepiece tubes,
provide high precision and superior operability.
Excellent optical performance through high resolution and aberration correction,
make them optimal for both image acquisition and observation.
This catalog presents the technical data for microscope units that are integrated with a variety of
equipment for biomedical research and testing.
For experimental equipment and inspection systems for biomedical research,
the optical performance of Nikon’s microscope units is always outstanding
3
Contents
System Diagram ���������������������������������������4
CFI60 Objectives
CFI Achromat Series for Brightfield/Phase Contrast (DL)/Apodized Phase Contrast (ADL) �����6
CFI Plan Achromat Series for Brightfield/Phase Contrast (DL) �����������������8
CFI Plan Fluor Series for Brightfield/Phase Contrast (DLL)/Apodized Phase Contrast (ADH) ��� 10
CFI Plan Fluor Series for Phase Contrast (DL) ����������������������� 11
CFI S Plan Fluor ELWD Series for Brightfield/Apodized Phase Contrast (ADM/ADL) ������ 12
Multi-Immersion Objective for Brightfield �������������������������� 12
CFI Plan ApochromatλSeries for Brightfield/Phase Contrast (DM) �������������� 13
CFI Plan Apochromat VC Series ������������������������������ 15
CFI Plan Apochromat IR Lens ������������������������������� 15
CFI Apochromat TIRF Series ������������������������������� 16
CFI SR Series �������������������������������������� 17
CFI HP Series �������������������������������������� 17
CFI Apochromat λS Series �������������������������������� 18
CFI S Fluor Series ������������������������������������ 19
Water Dipping Series ����������������������������������� 20
Nikon Advanced Modulation Contrast (NAMC) Series �������������������� 22
No Cover Glass Objective Lenses ����������������������������� 23
CFI Tube Lens Unit ������������������������������������ 24
Condensers ���������������������������������������� 26
Eyepieces ����������������������������������������� 28
Eyepiece Tubes �������������������������������������� 29
Camera Ports for Ergonomic Binocular Tube ������������������������� 30
Illumination Modules ������������������������������������ 31
Intermediate Modules ������������������������������������ 32
Contact Arm ���������������������������������������� 35
Nosepieces ���������������������������������������� 36
Camera Adapters �������������������������������������� 38
Main Body and Accessories ��������������������������������� 40
For information about the optical values of objectives and the dimensions of units and modules that are not included in this brochure, please contact Nikon.
4
System Diagram
Y-IM Magnification Module
DS-F2.5 F-mount Adapter 2.5x
DS-F F-mount Adapter
V-T Photo Adapter
C-mount TV Adapter 0.35x, 0.45x, 0.6x
C-mount TV AdapterVM2.5x
C-mount TV Adapter VM4x
C-DA C-mount Adapter
C-mount Adapter 0.7x
C-mount Adapter 0.55x
Y-TV TV Tube
Y-TV 0.55 TV Tube
C-TB Binocular Tube C-TF Trinocular Tube F C-TT Trinocular Tube T C-TE2 Ergonomic Binocular Tube
C-TEP2 DSC Port for Ergonomic Binocular Tube
C-TEPF2.5 DSC Port F2.5xfor Ergonomic Binocular Tube
CFI 12.5x CFI 10x CFI UW 10xCFI 15x
CFI60
NI-N7-E Motorized Septuple Nosepiece
NI-ND6-E Motorized DIC Sextuple Nosepiece
NI-N7-I Intelligent Septuple Nosepiece
NI-ND6-I Intelligent DIC Sextuple Nosepiece
D-ND6 DIC Sextuple Nosepiece
C-N6A Sextuple Nosepiece with Analyzer Slot
C-N6 ESD Sextuple Nosepiece ESD
2
3
4
2
3
4
EX.ADJ.
D-FL Epi-Fluorescence Attachment
Illumination Modules P. 31
Main Body and Control Box P. 40~41
Intermediate Modules P. 32~34
C-mount Camera F-mount Camera
Condensers P. 26~27
Nosepieces P. 36~37
Tubes and Ports P. 29~30
Contact Arm P. 35
Camera Adapters P. 38~39
Eyepieces P. 28
ObjectivesCFI Tube Lens Unit P. 6~23P. 24
D H
E
I
F
F
E
J
N
J
I
T
M
D
D
H
H
H
C
C
DD EE
LKM
N
M
K L
T
A
AA A
B
BA
B
CI-FL Epi-Fluorescence Attachment
NI-SAM Standard Arm
NIU-CAMContact Arm
NI-CTLB Control Box B
NI-SSR Substage
NI-CH Condenser Holder
ECLIPSE Ni-U
NI-SRCP Simple Remote Control Pad
F.STOP A.STOP
EX.ADJ.
E
D
E
D
Y-IDP Double Port
NI-FLEI EPI-Fluorescence Attachment
NI-FLT6-E Motorized Epi-fluorescence Cube Turret
NI-FLT6-I Intelligent Epi-fluoresce Cube Turret
NI-FLT6 Epi-fluorescence Cube Turret
C-FL Epi-fl Filter Cube
NI-LH Precentered Lamphouse
NI-BPU Back Port Unit
C-HGFI HG Fiber Illuminator “Intensilight”
C-HGFIE-C HG Controller
C-HGFIE Motorized HG Fiber Illuminator “Intensilight”
C-HGFIB HG 100W Adapter R
C-HGFIF15/30 HG Fiber 1.5 m/3.0 m
Dark Field CondenserOil 1.43 - 1.20
JAPAN
Dark Field CondenserDry 0.95 - 0.80
JAPAN
0.20.40.60.81.01.21.4
Achr-Apl N.A=1.4
Achromat Swing-out Condenser 2-100x
NI-CUD Universal Condenser Dry
C-C Achromat Swing-out Condenser 1-100x
X LWD Condenser
C-C Abbe Condenser
C-C Achromat Condenser
C-C Achromat Aplanatic Condenser
Darkfield Condenser Dry
C-C Slide Achromat Condenser 2-100x
Darkfield Condenser Oil
C-C Phase Contrast Turret Condenser *2
5
Y-IM Magnification Module
DS-F2.5 F-mount Adapter 2.5x
DS-F F-mount Adapter
V-T Photo Adapter
C-mount TV Adapter 0.35x, 0.45x, 0.6x
C-mount TV AdapterVM2.5x
C-mount TV Adapter VM4x
C-DA C-mount Adapter
C-mount Adapter 0.7x
C-mount Adapter 0.55x
Y-TV TV Tube
Y-TV 0.55 TV Tube
C-TB Binocular Tube C-TF Trinocular Tube F C-TT Trinocular Tube T C-TE2 Ergonomic Binocular Tube
C-TEP2 DSC Port for Ergonomic Binocular Tube
C-TEPF2.5 DSC Port F2.5xfor Ergonomic Binocular Tube
CFI 12.5x CFI 10x CFI UW 10xCFI 15x
CFI60
NI-N7-E Motorized Septuple Nosepiece
NI-ND6-E Motorized DIC Sextuple Nosepiece
NI-N7-I Intelligent Septuple Nosepiece
NI-ND6-I Intelligent DIC Sextuple Nosepiece
D-ND6 DIC Sextuple Nosepiece
C-N6A Sextuple Nosepiece with Analyzer Slot
C-N6 ESD Sextuple Nosepiece ESD
2
3
4
2
3
4
EX.ADJ.
D-FL Epi-Fluorescence Attachment
Illumination Modules P. 31
Main Body and Control Box P. 40~41
Intermediate Modules P. 32~34
C-mount Camera F-mount Camera
Condensers P. 26~27
Nosepieces P. 36~37
Tubes and Ports P. 29~30
Contact Arm P. 35
Camera Adapters P. 38~39
Eyepieces P. 28
ObjectivesCFI Tube Lens Unit P. 6~23P. 24
D H
E
I
F
F
E
J
N
J
I
T
M
D
D
H
H
H
C
C
DD EE
LKM
N
M
K L
T
A
AA A
B
BA
B
CI-FL Epi-Fluorescence Attachment
NI-SAM Standard Arm
NIU-CAMContact Arm
NI-CTLB Control Box B
NI-SSR Substage
NI-CH Condenser Holder
ECLIPSE Ni-U
NI-SRCP Simple Remote Control Pad
F.STOP A.STOP
EX.ADJ.
E
D
E
D
Y-IDP Double Port
NI-FLEI EPI-Fluorescence Attachment
NI-FLT6-E Motorized Epi-fluorescence Cube Turret
NI-FLT6-I Intelligent Epi-fluoresce Cube Turret
NI-FLT6 Epi-fluorescence Cube Turret
C-FL Epi-fl Filter Cube
NI-LH Precentered Lamphouse
NI-BPU Back Port Unit
C-HGFI HG Fiber Illuminator “Intensilight”
C-HGFIE-C HG Controller
C-HGFIE Motorized HG Fiber Illuminator “Intensilight”
C-HGFIB HG 100W Adapter R
C-HGFIF15/30 HG Fiber 1.5 m/3.0 m
Dark Field CondenserOil 1.43 - 1.20
JAPAN
Dark Field CondenserDry 0.95 - 0.80
JAPAN
0.20.40.60.81.01.21.4
Achr-Apl N.A=1.4
Achromat Swing-out Condenser 2-100x
NI-CUD Universal Condenser Dry
C-C Achromat Swing-out Condenser 1-100x
X LWD Condenser
C-C Abbe Condenser
C-C Achromat Condenser
C-C Achromat Aplanatic Condenser
Darkfield Condenser Dry
C-C Slide Achromat Condenser 2-100x
Darkfield Condenser Oil
C-C Phase Contrast Turret Condenser *2
6
CFI60 ObjectivesCFI Achromat Series for Brightfield/Phase Contrast (DL)Apodized Phase Contrast (ADL)Correction of chromatic aberration, spherical aberration and coma has been dramatically improved, with significantly better image flatness.
60.0
6
4.5
19.9
10
ø25
ø27
M25×0.75
C1
30˚
4 X /0.10∞/- WD 30
CFI Achromat 4x
M25×0.75
ø13.5ø16ø25
ø23.8ø27
4.5
60.0
6
7(W
D)
4.5
10
0.17
(CG
)
(52.
89)
36.5 10 X /0.25
∞/- WD 7.0
CFI Achromat 10x CFI Achromat DL 10x
M25×0.75
60.0
60.
17(C
G)
ø27ø23.8
ø25ø17ø15ø8.2
45˚
4.5
3.9(
WD
)
1032
10(3
.99)
45˚
20X/0.40LWD∞/0.17 WD 3.9
CFI Achromat LWD 20x CFI Achromat LWD DL 20x
ø7ø13ø17ø20ø25
60.0
6
ø27
M25×0.75
45˚
35˚
34.5
3.7
83
105
40X /0.65WD 0.65∞/0.17
CFI Achromat 40x CFI Achromat DL 40x
M25×0.75
ø27
ø7ø13ø17ø20ø25
ø23.8
35˚
(4.0
9)3
42.5
105
8
0.17
(CG
)0.
3(W
D)
60.0
6
45˚
60 X /0.80∞/0.17 WD 0.3
CFI Achromat 60x
M25×0.75
ø5.3ø13
ø16.6ø20ø25
60.0
6
ø27
20˚
45˚
58
34.5
105
2.16
100 X /1.25oil∞/0.17 WD 0.23
CFI Achromat 100x Oil CFI Achromat DL 100x Oil
7
M25×0.75
60.4
13.
111.
2
4.5
1036
6(4
.1)
ø27ø23.8
ø25ø17ø8.2
45˚45˚
Ph1 DL∞/ WD 3.11.2
20 X /0.40
M25×0.75
ø27.5ø23.8
ø29.5
ø30.5ø27.5ø19ø17
ø5.3ø13 0.
170.
2360
.06
531
4.53.5
85.8
4.7
(2.1
6)
0.5-1.25oil lris100 X
∞/0.17 WD 0.23
60.4
1
ø23.8ø27
ø13.5ø16ø25
4.5
1036
.54.
5
(53)
1.2
6.2(
WD
)
45̊
M25×0.75
Ph1 ADL∞/ WD 6.21.2
10 X /0.25
60.4
11.
2
ø23.8ø27
1033
.75.
84.
14.
5
ø12.1ø14.6ø19
ø21.5ø25
45̊
M25×0.75
2.1(
WD
)3.5
Ph1 ADL∞/ WD 2.11.2
40 X /0.55
CFI Achromat LWD DL 20x FCFI Achromat LWD ADL 20x F
CFI Achromat 100x Oil with iris diaphragm
CFI Achromat ADL 10x CFI Achromat LWD ADL 40x F
M25×0.754.5
60.4
11.
22.
1
(5.1
)5
252.
6
ø12ø20
ø25.5
ø34ø32
ø23.8
ø32
40˚
19.4
∞/ WD 2.1
0.41.6 1.2 0.8 02
0-2
40 X /0.55
CFI Achromat LWD 40x C CFI Achromat LWD DL 40x C
CFI Achromat LWD ADL 40x C
Code.No Objectives NAW.D. (mm)
Physical depthof focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRP00042 CFI Achromat 4x 0.10 30.0 27.5 5.5 72
MRP00102 CFI Achromat 10x 0.25 7.0 4.4 2.2 85
MRP20102 CFI Achromat DL 10x 0.25 7.0 4.4 2.2 85 Ph1
MRP40102 CFI Achromat ADL 10x 0.25 6.2 4.4 2.2 85 Cover glass thickness 1.2mm Ph1
MRP00202 CFI Achromat LWD 20x 0.40 3.9 1.72 1.1 90
MRP20202 CFI Achromat LWD DL 20x 0.40 3.9 1.72 1.1 90 Ph1
MRP00402 CFI Achromat 40x 0.65 0.65 0.65 0.55 130 Spring-loaded
MRP20402 CFI Achromat DL 40x 0.65 0.65 0.65 0.55 130 Spring-loaded Ph2
MRP00602 CFI Achromat 60x 0.80 0.3 0.43 0.367 130 Spring-loaded
MRP01902 CFI Achromat 100x Oil 1.25 0.23 0.27 0.22 130 Spring-loaded
MRP21902 CFI Achromat DL 100x Oil 1.25 0.23 0.27 0.22 130 Spring-loaded Ph3
MRP02902 CFI Achromat 100x Oil with iris diaphragm 0.5-1.25 0.23 0.27 0.22 130 Spring-loaded
MRP26202 CFI Achromat LWD DL 20x F 0.40 3.1 1.72 1.1 90 Cover glass thickness 1.2mm Ph1
MRP46202 CFI Achromat LWD ADL 20xF 0.40 3.1 1.72 1.1 90 Cover glass thickness 1.2mm Ph1
MRP46402 CFI Achromat LWD ADL 40xF 0.55 2.1 0.91 0.55 90 Cover glass thickness 1.2mm Ph1
MRP05422 CFI Achromat LWD 40x C 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm
MRP25422 CFI Achromat LWD DL 40x C 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm Ph2
MRP45422 CFI Achromat LWD ADL 40xC 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm Ph2
8
CFI60 ObjectivesCFI Plan Achromat Series for Brightfield/Phase Contrast (DL)Nikon's CFI Plan Achromat series provides incredible image flatness, with chromatic aberration corrected throughout the entire visible spectrum. These objectives are suitable not only for observation but also for capturing images.
60.0
6
4610
.75
ø31
ø30
M25×0.75
1X /0.04Plan UW
∞/-WD 3.2
CFI Plan Achromat UW 1x
60.0
652.4
5
5
ø20
ø30
M25×0.75
2X /0.06∞/-WD 7.5
Plan UW
CFI Plan Achromat UW 2x
60.0
6
19.9
104.
5
ø25
ø27
M25×0.75
4X /0.∞/-WD 3
Plan
CFI Plan Achromat 4x
60.0
6372.
45
10
ø19
ø12
ø25
ø27
M25×0.75
10X /0.25∞/-WD 10.5
Plan
45˚
45˚
CFI Plan Achromat 10x CFI Plan Achromat DL 10x
M25×0.75
60.0
6
1.22
(WD
)0.
17(C
G)
ø23.8
ø27
ø25
ø13
ø7
ø17
ø20
4.5
10 0.6
7.5
2.5
322
(4.6
7)
30˚
20 X /0.40Plan
∞/0.17 WD 1.2
CFI Plan Achromat 20x CFI Plan Achromat DL 20x
M25×
0.75
ø23.8
ø27
ø6
44.5
(2.5
)
0.17
(CG
)
60.0
6
10
52
55.5
(59.
33)
ø12.8
ø17
ø20
ø25
2
40 X /0.65Plan
∞/0.17 WD 0.56
CFI Plan Achromat 40x CFI Plan Achromat DL 40x
9
M25×0.75
0.17
ø23.8
ø14.5
ø19.5
ø22
60.0
6
ø27.5
5
ø5.5
0.18
49.7
5
4.25
45˚
30˚
1.5
(4.1
7)
50 X /0.90oil
Plan
∞/- WD 0.35
CFI Plan Achromat 50x Oil
M25×0.75
ø5.87
ø16
ø21
ø23.8
ø27.5
60.0
6
0.17
(CG
)0.
22(O
il)
47
57.5
(59.
67)
5
2.7
20˚
ø22.1
100XA/1.25Plan
∞/0.17 WD 0.2
CFI Plan Achromat 100x Oil CFI Plan Achromat DL 100x Oil
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRL00012 CFI Plan Achromat UW 1x 0.04 3.2 171.88 25 180
MRL00022 CFI Plan Achromat UW 2x 0.06 7.5 76.39 12.5 110
MRL00042 CFI Plan Achromat 4x 0.10 30.0 27.5 6.25 65
MRL00102 CFI Plan Achromat 10x 0.25 10.5 4.4 2.5 85
MRL20102 CFI Plan Achromat DL 10x 0.25 10.5 4.4 2.5 85 Ph1
MRL00202 CFI Plan Achromat 20x 0.40 1.2 1.7 1.25 85
MRL20202 CFI Plan Achromat DL 20x 0.40 1.2 1.72 1.25 85 Ph1
MRL00402 CFI Plan Achromat 40x 0.65 0.56 0.65 0.625 115 Spring-loaded
MRL20402 CFI Plan Achromat DL 40x 0.65 0.56 0.65 0.625 115 Spring-loaded Ph2
MRL01502 CFI Plan Achromat 50x Oil 0.90 0.35 0.51 0.44 160 Spring-loaded No cover grass
MRL01903 CFI Plan Achromat 100x Oil 1.25 0.2 0.27 0.22 160 Spring-loaded
MRL21903 CFI Plan Achromat DL 100x Oil 1.25 0.2 0.27 0.22 160 Spring-loaded Ph3
10
CFI60 ObjectivesCFI Plan Fluor Series for Brightfield/Phase Contrast (DLL)/Apodized Phase Contrast (ADH)
M25×0.75
ø16.5
45˚
(42.
69)
4
ø3017
.2(W
D)
0.17
(CG
)
60.0
6
4 X /0.13Plan Fluor
∞/- WD 17.2
CFI Plan Fluor 4x
M25×0.75
0.17
(CG
)
ø23.8ø28.2ø30
ø18
16.0
9(W
D)
60.0
6
45˚
(42.
4)
1.4
5
C0.6
10X /0.30DIC L/N1
Plan Fluor
∞/0.17 WD 16
JAPAN
CFI Plan Fluor 10x CFI Plan Fluor DLL 10x
M25×0.75ø23.8
ø28
ø8.5
ø15.5
45˚
5
0.17
(CG
)
60.0
6
2.1(
WD
)
ø20.5
51
45˚
(6.7
9)
20 X /0.50
Plan Fluor
∞/0.17 WD 2.1
CFI Plan Fluor 20x CFI Plan Fluor DLL 20x
M25×0.75ø23.8
ø27.5
ø28.2
ø30
45˚
5
156
.2
45˚
ø10
ø19
45˚
0.17
(CG
)0.
66(W
D)
60.0
6
(59.
23) 40X /0.75
DIC M/N2
Plan Fluor
∞/0.17 WD 0.66
JAPAN
CFI Plan Fluor 40x CFI Plan Fluor DLL 40x
M25×0.75
1240
4
(59.
61)
0.17(
CG)
0.28
(WD
)
60.0
6
ø6.92
22.5˚
ø22
ø25.8
ø28
C0.5
ø33.5
ø23.8
45˚
ø28
45˚82˚
5
40X /1.30oil
Plan Fluor
∞/0.17 WD 0.24
CFI Plan Fluor 40x Oil
M25×0.75ø23.8
ø5ø19.3ø21.5
40˚
70˚
60˚
0.17
(CG
)
60.0
6
5
32.5
59 2.1
ø30.5
ø31.5
ø26ø28
0.35
(WD
)
7.4
(59.
52)
60X/0.85Plan Fluor
/0.11-0.23 WD0.40-0
CFI Plan Fluor 60x
Featuring an extra-high transmission rate, especially in the ultraviolet wavelength, and flatness of field, this series is designed for fluorescence observation and imaging. These objectives can function as multi-purpose objectives for brightfield, fluorescence, simple polarizing, and DIC observations.
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRH00041 CFI Plan Fluor 4x 0.13 17.2 16.27 6.25 110
MRH00101 CFI Plan Fluor 10x 0.30 16.0 3.06 6.25 120
MRH10101 CFI Plan Fluor DLL 10x 0.30 16.0 3.06 2.5 120 Ph1
MRH00201 CFI Plan Fluor 20x 0.50 2.1 1.1 1.25 175
MRH10201 CFI Plan Fluor DLL 20x 0.50 2.1 1.1 1.25 175 Ph1
MRH00401 CFI Plan Fluor 40x 0.75 0.66 0.49 0.625 210 Spring-loaded
MRH10401 CFI Plan Fluor DLL 40x 0.75 0.66 0.49 0.625 210 Spring-loaded Ph2
MRH01401 CFI Plan Fluor 40x Oil 1.30 0.24 0.25 0.625 230 Spring-loaded,With stopper
MRH00602 CFI Plan Fluor 60x 0.85 0.4-0.31 0.38 0.417 265Spring-loadedCover glass correction 0.11–0.23mm
MRH02601 CFI Plan Fluor 60x Oil with iris diaphragm 0.50-1.25 0.22 0.27 0.417 230 Spring-loaded
MRH01902 CFI Plan Fluor 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper
MRH11902 CFI Plan Fluor DLL 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper Ph3
MRH41902 CFI Plan Fluor ADH 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper Ph3
MRH02902 CFI Plan Fluor 100x Oil with iris diaphragm 0.5–1.3 0.16 0.25 0.25 285 Spring-loaded
11
M25×0.75
ø30
ø23.8
ø27.7
ø26
ø20.8
ø17
ø6.1
60.0
6
0.17
(CG
)0.
16(O
il)
5
0.8
45˚
20˚4.5
(59.
71)
50.7 100X/1.30oil
∞/0.17 WD 0.16
Plan Fluor
M25×0.75
ø8ø29.2ø30.3ø32ø35
ø30ø33
ø34.5ø36
30˚75
˚
45˚
5
0.5
517
55
9.5
2.5
12(3
.16)
0.17
(CG
)
60.0
6
0.23
(oil)
ø27.5C0.5
60X/0.5-1.25oil Iris
1.25 0.5
Plan Fluor
∞/0.17 WD 0.22
CFI Plan Fluor 100x Oil CFI Plan Fluor DLL 100x OilCFI Plan Fluor ADH 100x Oil
M25×0.75
ø5.2
ø23.4
ø32
ø32.4
ø33.4
ø32
ø23.8
8˚
38.8
3.5(5
9.7)
3.5
2.7 6.5
2.2
0.17
(CG
)0.
16(O
il)
60.0
6
45˚
17˚
5
ø19.8
ø27
7.1
100X/0.5-1.3oil IrisPlan Fluor
∞/0.17 WD 0.16
CFI Plan Fluor 100x Oil with iris diaphragm
CFI Plan Fluor 60x Oil with iris diaphragm
4X / 0.13 Plan Fluor
PhL DL ∞/1.2 WD 16.5
M25×0.75
ø30
60.4
1
42.8
55.
3
45˚
CFI Plan Fluor DL 4x
10X / 0.30 Plan Fluor
Ph1 DL ∞/1.2 WD 15.2
M25×0.75
ø30
60.4
1
1.2
445.
3
45˚
6
CFI Plan Fluor DL 10x
CFI Plan Fluor Series for Phase Contrast (DL)These objectives have more contrast than the DLL objectives for enhanced contrast with tissue culture specimens. These objectives are designed for use on inverted microscopes and therefore their markings are upside-down for easy reading.
Code.No Objectives N.A.W.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRH20041 CFI Plan Fluor DL 4x 0.13 16.5 16.27 6.25 105 Cover glass thickness 1.2mm PhL
MRH20101 CFI Plan Fluor DL 10x 0.30 15.2 3.06 2.50 120 Cover glass thickness 1.2mm Ph1
12
CFI60 Objectives
ø8
ø33
ø28
1910
.412
.514
5.3
3.7
8˚
M25×0.75
20X / 0.75 Mlmm Plan Fluor
∞ / - WD 0.35
Oil G W0.17 0
ø36
ø34.5
ø30.6
3˚
30˚
40˚
CFI Plan Fluor 20x MI
M25×0.75ø32
ø16ø25.5ø32.5ø34
35˚
5˚
C0.8
5
12.819
.55
22.2
60.4
1
1.2(
CG
)7.
4(W
D)
C0.5
C0.5
(5.1
1)
7˚
20X /0.45LWDS Plan Fluor
∞/0-2 Ph1 ADM
CFI S Plan Fluor ELWD 20x C CFI S Plan Fluor ELWD ADM 20x C
M25×0.75
Front focal point
Back focal pointPupil
position
ø32
ø10.5
ø22
ø27.5
ø32.5
ø34
30˚
C0.5
1.2(
CG
)
60.4
1
12.8
519
.55
24.7
1.8
(5.1
1)
3.1(
WD
)
5˚
5.2
C0.5
C0.5
7˚
539
.515
.9
10.9
40X /0.60LWDS Plan Fluor
DIC N1
0.171.22 0
CFI S Plan Fluor ELWD 40x C CFI S Plan Fluor ELWD ADM 40x C
M25×0.75
ø8.8
ø17
ø19.2
ø22
ø29.5
ø31.5
35˚35˚
7˚
C0.5
C0.5
ø309˚
5
3.8
17.5
5
5.2
28
3.06
(3.8
5) 1.2(
CG
)1.
8(W
D)
60.4
1
60X /0.70WDS Plan Fluor
∞/0.1-1.3 WD2.6-1.8
0.171.0 0.7 0.41.2
CFI S Plan Fluor ELWD 60x C CFI S Plan Fluor ELWD ADL 60x C
Multi-Immersion Objective for Brightfield
Newly developed broadband multilayer coating realizes high transmittance from near-ultraviolet to near-infrared wavelengths, with improved chromatic correction. The correction collar ring allows these objectives to be used with a diverse range of culture vessels and specimen thicknesses. High-quality images with no aberrations can be obtained under a broad range of illumination techniques.
This multi-purpose immersion objective has a higher numerical aperture and superior contrast. The use of immersion media helps eliminate surface reflections and provides an image with higher contrast. It is an excellent objective for fluorescence, brightfield, and DIC observation techniques. This unique objective has a correction collar that allows it to be used with immersion oil, glycerin, and water (with and without a cover glass).
CFI S Plan Fluor ELWD Series for Brightfield/Apodized Phase Contrast (ADM/ADL)
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRH08230 CFI S Plan Fluor ELWD 20x C 0.45 8.2–6.9 1.36 1.1 195 Cover glass correction 0–2mm
MRH48230 CFI S Plan Fluor ELWD ADM20x C 0.45 8.2–6.9 1.36 1.1 195 Cover glass correction 0–2mm Ph1
MRH08430 CFI S Plan Fluor ELWD 40x C 0.60 3.6–2.8 0.76 0.55 195 Cover glass correction 0–2mm
MRH48430 CFI S Plan Fluor ELWD ADM40x C 0.60 3.6–2.8 0.76 0.55 195 Cover glass correction 0–2mm Ph2
MRH08630 CFI S Plan Fluor ELWD 60x C 0.70 2.6–1.8 0.56 0.367 210 Cover glass correction 0.1–1.3mm
MRH48600 CFI S Plan Fluor ELWD ADL60x C 0.70 2.6–1.8 0.56 0.367 210 Cover glass correction 0.1–1.3mm Ph2
Code.No Objective NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRH07241CFI Plan Fluor 20x MI
0.75 0.35(Oil) 0.74 1.25 300
Multi-immersionOil-glycerine-waterSpring-loadedCover glass correction 0–0.17mm
13
M25×0.75ø23.8
ø30
ø19
45˚
5(5
1.39
)
60.0
6
0.17
(CG
)8.
5(W
D)
ø29
0.5
C0.5
2X /0.10
Plan Apo λ
∞/- WD 8.5
CFI Plan Apochromatλ2x
M25×0.75
5(3
9.89
)
ø18
30˚ 60
.06
20(W
D)
0.17
(CG
)
ø23.8
ø30
ø28.2
0.8
4X /0.20
Plan Apo λ
∞/- WD 20
CFI Plan Apochromatλ4x
M25×0.75ø23.8
ø30
5(5
5.89
)
60.0
6
4(W
D)
0.17
(CG
)
45˚
ø15
ø27.7
0.8
10X /0.45
Plan Apo λ
∞/0.17 WD 4.0
CFI Plan Apochromatλ10x
M25×0.75
0.17
(CG
)
60.0
6
ø27
ø23.8
ø25.8
ø21.8
5
3.5
40
ø27
ø28
0.5
49.5
ø6.5
45˚
(58.
89)
1(W
D)
60˚
ø31
ø32.5
45˚
20X /0.75
Plan Apo λ
∞/0.17 WD 1.0
CFI Plan Apochromatλ20x CFI Plan ApochromatλDM 20x
M25×0.75ø23.8
ø27
5
0.5
7.5
ø18.8
0.17
(CG
)
60.0
6
18.9
11.1
ø8
40˚
16.8
40˚
ø21
70˚
ø33
5˚
ø34.5
(59.
68)
0.21
(WD
)
ø28.5
ø30.8
40X /0.95Plan Apo λ
∞/0.11-0.23
0.11 0.17 0.23
WD0.25-0.17
CFI Plan Apochromaλ40x CFI Plan ApochromatλDM 40x
M25×0.75ø23.8
ø27
0.5
5
70˚
5˚
ø6.5
0.17
(CG
)
60.0
6
18
40˚
40˚
ø18.5
0.15
(WD
)
(59.
74)
ø28.5
ø33
ø34.5
ø30.6
811
.616
.4
ø21
60X /0.95Plan Apo λ
∞/0.11-0.23 WD0.20-0.11
CFI Plan Apochromatλ60x CFI Plan ApochromatλDM 60x
The ultra-low, refractive index coating technology “Nano Crystal Coat” enables the λ series’ remarkably high transmission throughout a broad range of wavelengths up to the near-IR region. As chromatic aberrations are corrected throughout a wavelength range from visible to near infrared, bright, high-contrast images are captured during long-wavelength imaging, which is less phototoxic to live-cells. Moreover, the unmatched chromatic aberration correction, resolution and image flatness of the λ series ensure the capture of high-quality brightfield images.
CFI Plan ApochromatλSeries for Brightfield/Phase Contrast (DM)
14
M25×0.75
0.17
(CG
)
60.0
6
ø25.8
5
3.5
40
ø27
0.13
(WD
)
(59.
76)
35˚
ø9.17
ø23.8
ø27
ø28
0.5
60˚
ø31
32.5
45˚
55.8
60X /1.40oil
Plan Apo λ
∞/0.17 WD 0.13
CFI Plan Apochromatλ60x Oil CFI Plan ApochromatλDM 60x Oil
M25×0.75
0.17
(CG
)0.
13(W
D)
60.0
6
ø25.8
ø9.17
5(5
9.76
)
3.5
40
ø27
45˚
35˚
ø23.8
ø27
ø28
0.5
60˚
ø32.5
ø31
56
100X /1.45oil
Plan Apo λ
∞/0.17 WD 0.13
CFI Plan Apochromatλ100x Oil CFI Plan ApochromatλDM 100x Oil
CFI60 ObjectivesCFI Plan Apochromatλ Series for Brightfield/Phase Contrast (DM)
Code.No Objectives NAW.D. (mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks Phase ring
MRD00025 CFI Plan Apochromat λ2x 0.10 8.5 27.50 12.5 180
MRD00045 CFI Plan Apochromat λ4x 0.20 20.0 6.88 6.25 180
MRD00105 CFI Plan Apochromat λ10x 0.45 4.0 1.36 2.5 220
MRD00205 CFI Plan Apochromat λ20x 0.75 1.0 0.49 1.25 240 Spring-loaded
MRD30205 CFI Plan Apochromat λDM20x 0.75 1.0 0.49 1.25 240 Spring-loaded Ph2
MRD00405 CFI Plan Apochromat λ40x 0.950.21
(0.25-0.17)0.30 0.625 270
Spring-loadedCover glass correction 0.11–0.23mm
MRD30405 CFI Plan Apochromat λDM40x 0.950.21
(0.25-0.17)0.30 0.625 270
Spring-loadedCover glass correction 0.11–0.23mm
Ph2
MRD00605 CFI Plan Apochromat λ60x 0.950.15
(0.20-0.11)0.30 0.417 285
Spring-loadedCover glass correction 0.11–0.23mm
MRD30605 CFI Plan Apochromat λDM60x 0.950.15
(0.20-0.11)0.30 0.417 285
Spring-loadedCover glass correction 0.11–0.23mm
Ph2
MRD01605 CFI Plan Apochromat λ60x Oil 1.40 0.13 0.21 0.417 285 Spring-loaded
MRD31605 CFI Plan Apochromat λDM60x Oil 1.40 0.13 0.21 0.417 285 Spring-loaded Ph3
MRD01905 CFI Plan Apochromat λ100x Oil 1.45 0.13 0.20 0.25 245 Spring-loaded
MRD31905 CFI Plan Apochromat λDM100x Oil 1.45 0.13 0.20 0.25 245 Spring-loaded Ph3
15
M25×0.75
60.0
6
0.17
(CG
)
ø23.8ø27.5ø30ø33
10˚
5
0.5
48.5
45˚
4(5
.828
)
30˚
C0.5
ø7ø21.4ø25.8ø27.3
1.06
(WD
)
20X /0.75DIC N2
Plan Apo VC
∞/0.17 WD 1.0
CFI Plan Apochromat VC 20x
M25×0.75ø31ø33
ø34.5
ø8
ø26ø31.4
56.
258.
75(3
.39)
22.5
0.17
(CG
)0.
3(w
ater
)
60.0
6±0.
01
8.7
6.2
3.8
ø22.8
60XA/1.20 WIPlan Apo VC
∞/0.15-0.18 WD0.31-0.28
15 16 17 18 19
CFI Plan Apochromat VC 60x WI
M25×0.75ø23.8
ø6.9
ø28
25˚
55.5
5
ø22
0.17
(CG
)0.
13(W
D)
60.0
6
45
ø35
C2
31.6
ø27.5ø30
0.5
C1
(59.
74) 100X /1.40oil
Plan Apo VC
∞/0.17 DIC N2
JAPAN
CFI Plan Apochromat VC 100x Oil
CFI Plan Apochromat VC Series
CFI Plan Apochromat IR Lens
These objectives provide perfect correction of chromatic aberrations in the visible light range and excellent resolution throughout the view field, and are the perfect choice for multi-stained, fluorescence specimens, and for brightfield and DIC observation. In addition to the correction range of the conventional Plan Apochromat series (435–660nm), axial chromatic aberration has been corrected up to the violet range (405nm), making these objectives highly effective for confocal applications.The 60x water-immersion type features high spectral transmittance, even in the 360nm wavelength ultra-violet range, making it perfect for the fluorescence observation of living organisms.
With the world’s highest NA (1.27) for a 60x water immersion objective, this lens achieves a high level of resolution and sharp image acquisition. It corrects chromatic aberration up to 1,064 nm.
0.17
(CG
)
60.0
6
5
ø9
ø25.1ø33ø36
ø34.5M25×0.7510˚
45˚
3
ø20
0.17
(wat
er) 6.9
20.5
20.1
6
ø23.8
9.5
20˚
(3.1
5)
60Plan Apo IR
X /1.27 WI
0.19 0.17 0.15
∞/0.15-0.19 WD0.18-0.16
CFI Plan Apochromat IR 60x Wl
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRD70200 CFI Plan Apochromat VC20x 0.75 1.0 0.49 1.25 170 Spring-loaded
MRD07602 CFI Plan Apochromat VC60x WI 1.200.29
(0.31–0.28)0.25 0.367 220
Spring-loadedCover glass correction 0.15–0.18mm
MRD01901 CFI Plan Apochromat VC100x Oil 1.40 0.13 0.21 0.25 300 Spring-loaded
Code.No Objectives (magnification) NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRD07650 CFI Plan Apochromat IR60x Wl 1.27 0.18–0.16 0.23 0.367 250 Spring-loadedCover glass correction 0.15–0.19mm
16
M25×0.75
ø9
ø29
ø32
ø23.8
ø27.8
ø31.5
ø33.5
C0.3
C1
5
0.3
16.2
80˚
827
5(3
.2)
C0.5
C1
0.17
(CG
)0.
13(
Oil)
60.0
6
28˚
35.5
60X /1.49oil
23˚CCG 0.19 0.17 0.13
Apo TIRF
∞/0.13-0.21 WD 0.12
CFI Apochromat TIRF 60x Oil
M25×0.75
60.0
6
ø35.5
0.17
(CG
)
ø9
ø29
ø32
C0.3
C1
(3.2
)5
258
18.2
32˚
50.3
ø33.5
ø23.8
ø27.8
ø31.5
80˚
C0.5
C1
0.13
(Oil)
ø27
1.5
45˚ø23
100X /1.49oil
23˚CCG 0.19 0.17 0.13
Apo TIRF
∞/0.13-0.20 WD 0.12
CFI Apochromat TIRF 100x Oil
CFI Apochromat TIRF SeriesBecause of the unprecedented high NA of 1.49—for use with a standard coverslip and immersion oil—these objectives enable the acquisition of high-resolution, high S/N ratio images during TIRF observation, epi-fluorescence and confocal observation, as well as DIC observation. The spherical aberration correction ring reduces deterioration in image quality caused by deviations in cover glass thickness or temperature fluctuations, and provides optimal optical performance at both 23°C and 37°C. The 100x objective can be optimally applied for laser tweezers microscopy.
CFI60 Objectives
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRD01691 CFI Apochromat TIRF 60x Oil 1.49 0.12 0.19 0.367 265 Cover glass correction0.13–0.19mm (23C˚)0.15–0.21mm (37C˚)
MRD01991 CFI Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 275 Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)
17
M25×0.75
0.17
(CG
)
60.0
6
5ø9
ø33
ø36
ø34.5
10˚
ø20
0.17
(wat
er)
20.1
6
ø23.8
9.5
ø26.6ø28.5
1.9
ø24.7
45˚
(3.2
2)
20˚
60X /1.27 WISR Plan Apo IR
0.19 0.17 0.15
∞/0.15-0.19 WD0.18-0.16
CFI SR Plan Apochromat IR 60x Wl
M25×0.75
60.0
6
ø35.5
0.17
(CG
)
ø9
ø29ø32
C0.3
C1
(3.2
)5
258
18.2
32˚
50.3
ø33.5
ø23.8ø27.8ø31.5
80˚
C0.5
C1
0.13
(Oil)
ø27
1.5
45˚
ø23
100X /1.49oil
23˚CCG 0.19 0.17 0.13
HP Apo TIRF
∞/0.13-0.20 WD 0.12
M25×0.75
60.0
6
ø35.5
0.17
(CG
)
ø9ø29ø32
C0.3
C1
(3.2
)5
258
18.2
32˚
50.3
ø33.5
ø23.8ø27.8ø31.5
80˚
C0.5
C1
0.13
(Oil)
100X /1.49oil
23˚CCG 0.19 0.17 0.13
SR Apo TIRF
∞/0.13-0.20 WD 0.12
CFI HP Apochromat TIRF 100x Oil
CFI SR Apochromat TIRF 100x Oil
CFI SR Series
CFI HP Series
The SR (super resolution) objectives have been designed to provide superb optical performance with Nikon’s super-resolution microscope N-SIM. The adjustment and inspection of lenses using wavefront aberration measurement have been applied to yield optical performances with the lowest possible asymmetric aberration.
The CFI HP (High Power) objectives are optimized for the super resolution microscope N-STORM, which uses high power lasers to blink fluorophores at ultra-high speed. Thanks to the improved correction of axial chromatic aberration, these lenses enable high precision 3D multi-color fluorescence imaging.
M25×0.75ø23.8
ø6.9
ø28
25˚
55.5
5
ø22
0.17
(CG
)0.
13(W
D)
60.0
6
45
ø35
C2
31.6
ø27.5ø30
0.5
C1
(59.
74) 100X /1.40oil
HP Plan Apo VC
∞/0.17 DIC N2
JAPAN
CFI HP Plan Apochromat VC 100x Oil
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRY10060 CFI SR Plan Apochromat IR 60x Wl 1.270.17
(0.18–0.16)0.23 0.37 250
Spring-loadedCover glass correction 0.15–0.19mm
MRY10059 CFI SR Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 275 Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)
Code.No Objectives NA W.D. (mm)Physical depth of
focus (µm)Actual field
of view (ømm)Weight (g) Remarks
MRD01902 CFI HP Plan Apochromat VC 100x Oil 1.40 0.13 0.21 0.25 300 Spring-loaded
MRD01993 CFI HP Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 300Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)
18
CFI Apochromat λS SeriesThe λS series objectives provide chromatic aberration correction over a wide wavelength range from 405nm, and are powerful enough for spectral imaging and simultaneous multi-wavelength acquisition. The LWD 20x/40x WI λS lens has an extremely wide chromatic aberration correction range of 405nm to 950nm and is suitable for multiphoton observation. The 40x WI λS lens has an NA of 1.25, the world’s highest for a 40x water immersion objective. The 60x oil λS lens offers high level chromatic aberration correction across the whole visible range and is a powerful tool for confocal spectral imaging and photostimulation.
0.110.170.23
M25×0.75
ø31
ø35
ø37
ø34.6ø29.5ø24.3
ø9
60.0
6
5
6.5
19.5
7
80˚35˚
1
5.4
11.5
8
58.9
Apo LWD
∞/0.11-0.2320X/0.95 WI λS
WD 0.95
CFI Apochromat LWD 20x WI λS
M25×0.75
0.17
(CG
)
60.0
6
0.18
(wat
er)
21.5
616
.5
3.5
9.5
(2.9
04)
5
ø35.5
ø37.5
ø9ø19.3ø24.8ø33.5
11˚
22˚
45˚
11.8
Apo 40X /1.25 λS
0.19 0.17 0.15
∞/0.15-0.19 WD0.20-0.16
CFI Apochromat 40x WI λS
M25×0.75
Front focal point
Back focal point Pupil
position
ø9ø25.3
ø30
ø29ø31.5ø33.5
30˚
0.5
517
7.5
ø26.5
(5.2
9)
45˚
60.0
6
0.17
(CG
)0.
6(w
ater
)
45˚
245.
5
25.4
29.6
20.4
5
Apo 40Apo LWD
X /1.15 λS
0.19 0.17 0.15
∞/0.15-0.19 DIC NS
CFI Apochromat LWD 40x WI λS
M25×0.75
0.17
(CG
)
60.0
6
0.14
(Oil)
ø32
ø8
ø21.5
ø26
ø28.5
ø30.5
ø23.8
30˚
(59.
74)
5
45˚
45˚
3610
.25
4.25
ø27
55.0
8
60X /1.40 oil λS
Apo
∞/0.17 WD 0.14
CFI Apochromat 60x Oil λS
CFI60 Objectives
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRD77200 CFI Apochromat LWD20x WI λS 0.95 0.95 0.41 1.1 245 Cover glass correction 0.11–0.23mm
MRD77400 CFI Apochromat 40x WI λS 1.250.18
(0.20–0.16)0.23 0.55 260
Spring-loadedCover glass correction 0.15–0.19mm
MRD77410 CFI Apochromat LWD40x WI λS 1.15 0.6 0.28 0.55 205 Spring-loadedCover glass correction 0.15–0.19mm
MRD71600 CFI Apochromat 60x Oil λS 1.40 0.14 0.21 0.367 180 Spring-loaded
19
5.3
8.2
1.2
1238
.564
ø32
ø8
ø19
ø24
ø30
M25×0.75
10X / 0.50∞/ WD 1.20.17
S Fluor
M25×0.750.
17(C
G)
15.5
(WD
)
60.0
6(44.
39)
5
ø23.8
ø30
ø19.7
35˚
4X /0.20
S Fluor
∞/- WD 15.5
CFI S Fluor 10xCFI S Fluor 4x
1
5.3
5.9
1241
64.2
ø9
ø22ø25
ø33.5
ø30
M25×0.75
20X / 0.75∞/ WD 1.00.17
S Fluor
CFI S Fluor 20x
527
8.5
3.1
10.5
10.5
ø7.5
ø18
ø26
ø33
ø34.5
ø33
ø30
M25×0.75
11 14 17 20 23
40X / 0.9/ WD 0.30.11-0.23
Fluor
CFI S Fluor 40x
512
376.
54.
2
ø32
ø30
ø8.3
ø20
ø23.8
M25×0.75
40X / 1.30∞/ WD 0.220.17
S Fluor
Oil
CFI S Fluor 40x Oil
532
7.5
7.5
9.8
2.9
64.7
ø32
ø30
ø6
ø17
ø24
ø29.5
M25×0.75
1.3 0.7
100 X /0.5-1.3 oilS Fluor
∞/ WD 0.20.17
CFI S Fluor 100x Oil
CFI S Fluor SeriesThis CFI S Fluor series ensures a high transmission rate of ultraviolet wavelengths down to 340nm for fluorochromes like indo-1 and fura-2. Also, these objectives have improved S/N ratios for short wavelengths and have high NA, making the fluorescence images they produce significantly sharper and brighter.
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRF00040 CFI S Fluor 4x 0.20 15.5 6.88 5.5 115
MRF00100 CFI S Fluor 10x 0.50 1.2 1.1 2.2 275 Spring-loaded
MRF00200 CFI S Fluor 20x 0.75 1.0 0.49 1.1 275 Spring-loaded
MRF00400 CFI S Fluor 40x 0.90 0.3 0.34 0.55 285Spring-loadedCover glass correction 0.11–0.23mm
MRF01400 CFI S Fluor 40x Oil 1.30 0.22 0.25 0.55 275 Spring-loaded,With stopper
MRF02900 CFI S Fluor 100x Oil with iris diaphragm 0.50-1.30 0.2 0.25 0.22 285 Spring-loaded
20
• Long WD and high NA at any magnification.• Sharper tips and broad approach angles provide improved accessibility for manipulator control.• Aberrations are corrected even in the infra-red range with the high-magnification objectives, making them
suitable for multi-photon imaging using infra-red light.• 100xW objective with a correction ring that corrects spherical aberration induced by imaging depth or
temperature fluctuations. With excellent infra-red transmission, this lens assures best quality images of even a thick specimen.
• The 16x objective is ideal for patch-clamp experiments when combined with the FN1 microscope and magnification module. With its excellent IR transmission and high NA, this lens is suitable for IR-DIC observation and provides superb image quality in combination with confocal microscopes.
• The 25xW MP objectives provide the high numerical aperture of 1.10 and a long 2.0 mm working distance, and are ideal for deep multiphoton imaging with a collar that corrects spherical aberrations depending on the depth of the specimen. Chromatic aberrations are effectively corrected from ultraviolet to infrared wavelengths, and transmission is increased by applying a Nano Crystal Coat.
M25×0.75
1.5
5
ø23.8
ø28
43
55.9
(56.
9)
5
60˚
ø5.5
ø6.5
ø18.5
ø21.5
ø24
60˚
C0.5
45˚
60.5
3.5(
wat
er)
10X /0.3W
Plan Fluor
DIC N1
∞/0 WD 3.5
JAPAN
CFI Plan Fluor 10x W
Lens
M25×0.75
ø6
ø17.8
ø22.8
ø25
44.2
50
57.2
5
5.9
C1.2
C0.5
2(W
D)
60.5
5(1
.25)
C0.75
35.2
32.9
ø29.5
ø23.8
ø5
20FluorX /0.50W
DIC M∞/ WD 2.00
CFI Fluor 20x W
M25×0.75
(58.
5)
55
(4.5
)
ø4
ø19ø23
ø25.5
ø29
45˚
60.5
2(w
ater
)
5
45˚
50˚
32.7
11.3
ø11.7
ø23.8
9.55
ø12.5
40FluorX /0.80W
DIC M∞/ WD 2.00
CFI Fluor 40x W
Water Dipping SeriesCFI60/CFI75 Objectives
21
HL
M25×0.75
Index
Lens
60.5
ø35.5
ø28
ø27.5
5
1 6.5
ø35
ø22.8
ø10.8
C180˚
20˚30˚
ø8.3
ø11.6
0.6
4.2
45˚
13
5
C0.3
C0.5
C0.5
ø34
16.1
ø32.5
17.9
9.1
2.5
2.6(
wat
er)
Plan 100X /1.10W∞/0 WD 2.5 DIC H/N2
M25×
0.75 ø29
v5.5
ø19.2ø23
ø25.5
85.
5(1
)
(58.
5)5
60.5
2(W
D)
40˚
30˚
32.7
11.3
ø23.8
9.55
ø9
60FluorX /1.00W
DIC H∞/ WD 2.00
CFI Plan 100x W
CFI Fluor 60x W
M32×0.75ø30.8ø35
45˚
2.5
457
.566
.8(7
2)5
C1
45˚
45˚
ø6ø15ø16ø24ø29
3(w
ater
)7516 X /0.80W
LWD
∞/0 WD 3.0DIC N2
M25×0.75ø23.8
ø28
45˚
51.
5
50
53.3
(57)
45˚
45˚
50˚
5
ø6
ø13.7
ø19
ø20.4
ø24
60.5
3.5(
wat
er)
40X /0.80W
NIR Apo
DIC N2∞/0 WD 3.5
JAPAN
CFI75 LWD 16xW
CFI Apochromat 40xW NIR
M32×0.75
Lens
59.
611
.66.
518
.318
.56.
05(2
.41)
ø38
ø30.8
ø36
ø34.1
ø29.4
2.04
3(W
D)
ø6.1
33˚
ø12.9
75
ø34.5
45˚
ø31.5
45˚
ø35.6
45˚
ø13.6
Apo LWD 25X /1.10W
CG
SAMPLE DEEP
0.17 0.12
∞/0-0.17 WD 2.0
M25×0.75ø23.8
ø28
45˚
1.5
5
52.8
57
(57.
6)5
C1
40˚
30˚
ø8.5
ø10.6
ø11.4
ø19.2
ø24
60.5
2.8(
wat
er)(0
.93)
(3.2
7)
60X /1.0W
NIR Apo
DIC N2∞/0 WD 2.8
JAPAN
CFI75 Apochromat 25xW MP CFI75 Apochromat 25xW MP 1300
CFI Apochromat 60xW NIR
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRH07120 CFI Plan Fluor 10x W 0.30 3.5 4.07 2.5 110 No cover grass
MRF07220 CFI Fluor 20x W 0.50 2.0 1.47 1.25 130 No cover grass
MRF07420 CFI Fluor 40x W 0.80 2.0 0.57 0.625 210 No cover grass
MRF07620 CFI Fluor 60x W 1.00 2.0 0.37 0.367 210 No cover grass
MRD07420 CFI Apo 40xW NIR 0.80 3.5 0.57 0.55 120 No cover grass
MRD07620 CFI Apo 60xW NIR 1.00 2.8 0.37 0.367 125 No cover grass
MRL07920 CFI Plan 100x W 1.10 2.5 0.3 0.22 225With Correction ring 0-0.13mmNo cover grass
MRP07220 CFI75 LWD 16xW 0.80 3.0 0.57 1.375 195 No cover grass
MRD77220 CFI75 Apo 25xW MP 1.10 2.0 0.3 0.88 360With Correction ringCover glass correction 0–0.17mm
MRD77225 CFI75 Apo 25xW MP 1300 1.10 2.0 0.3 0.88 360With Correction ringCover glass correction 0–0.17mm
22
Nikon has developed dedicated objectives for advanced modulation contrast. Colorless and transparent samples can be observed in high relief with a plastic dish, which is not possible in DIC observation. The direction of contrast can be matched to S Plan Fluor ELWD NAMC objectives, thereby allowing optimal contrast selection for techniques like microinjection and ICSI.
M25×0.75
ø13.5
ø25
ø27.8
ø27
ø23.8
(6.2
3)1.
2
60.4
15
2.48
ø16
45˚
27.5
518
10 X /0.25
NAMC1
∞/1.2 WD 6.2
M25×0.75
1.2(
CG
)7.
4(W
D)
60.4
1
55
2(3
.31)
ø15ø22.8ø24.5
ø32ø34ø37
ø35
ø27.5ø31
35˚45˚45˚
9.5
3.5
5.5 10
.5
83˚
5.9
6.6
ELWD20X/0.45NAMC2
S Plan FluorCLAMP
2 0.171.2
CFI NAMC 10x
CFI S Plan Fluor ELWD NAMC 20xC
M25×0.7560
.41
1.2(
CG
)3.
1(W
D)
ø8.5
ø14.6
ø16
ø26
ø27.8
ø27
4.5
185
(56.
11)
45˚45˚
29 LWD20X/0.40
NAMC2
∞/1.2 WD 3.1
M25×0.75
1.2(
CG
)
ø32ø34
5
9.3
6.7
52
60.4
1
ø39.5
30˚
ø11ø27.5ø30.5
ø37.5
45˚
3.1(
WD
)
8.7
5.5
49.
5
(56.
11)
ø33.5
82˚
ELWD40X/0.60NAMC3
S Plan Fluor
2 0.171.2
CLAMP
CFI LWD NAMC 20xF
CFI S Plan Fluor ELWD NAMC 40xC
M25×0.75
ø12
ø20
ø25.5
15
0.5
85˚
60.4
1
1.2(
CG
)2.
1(W
D)
4.5
0.4
ø28
ø29
ø32
80˚19
57
41.
6
(57.
11)
ø34
ø3140˚
ø26.5
45˚
LWD40X/0.55
NAMC3
2 1.6 1.2 0.8 0.4 0
∞/0-2 WD 2.7-1.7
M25×0.75
220.
7
6.5
5
ø28.5ø29.5ø34
ø35.5
ø19.8ø24.8ø27
ø32.5
ø14.5
0.9(
CG)
60.3
1
(58
.2)
16˚
45˚(8
.2)
26˚
1.2(
WD
)
ø23.8
522
.3LWD IMSI 100X/0.85
1.3 0.60.9
∞/0.6-1.3 WD 0.95
CFI LWD NAMC 40xC
CFI Plan LWD IMSI 100xC
Nikon Advanced Modulation Contrast (NAMC) SeriesCFI60 Objectives
Code.No Objectives NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRP60105 CFI NAMC 10x 0.25 6.2 4.4 2.2 165 Cover glass thickness 1.2mm
MRP66205 CFI LWD NAMC 20xF 0.40 3.1 1.72 1.1 180 Cover glass thickness 1.2mm
MRP65425 CFI LWD NAMC 40xC 0.55 2.1
(2.7–1.7)0.91 0.55 250 Cover glass correction 0–2mm
MRH68200 CFI S Plan Fluor ELWD NAMC 20xC 0.45 7.4 1.36 1.1 225 Cover glass correction 0–2mm
MRH68400 CFI S Plan Fluor ELWD NAMC 40xC 0.60 3.1 0.76 0.55 275 Cover glass correction 0–2mm
MRL65900 CFI Plan LWD IMSI 100xC 0.85 1.15
(1.3-0.95)0.38 0.25 260
Spring-loadedCover glass correction 0.6–1.3mm
23
No Cover Glass Objective Lenses
M25×0.75
60
0.53
550
.53.
5(5
.5)
ø13.8
ø7.5
ø18.8
ø30
45˚
45˚
ø23.8
ø24
40 X /0.65NCG
Plan
∞/0 WD 0.48
CFI Plan Achromat NCG 40x
M25×0.75
60
1.08
547
.5
4.5
3.5
(3.4
2) ø8.8
ø18
ø21.8
ø24
ø30
ø23.8
100X /0.90NCG
Plan
∞/0 WD 1.0
CFI Plan Achromat NCG 100x
ø23.8
ø28ø22
11˚
0.17
4(O
il)
25˚
ø6.5
60
ø31
C1
45˚
ø27.5ø30ø35
M25×0.75
100X /1.40oilPlan Apo
∞/0 DIC N2
JAPAN
50.5
12.9
31.6
10.5
(4.326)
CFI Plan Apochromat NCG 100x Oil
Code.No Objectives (magnification) NAW.D.(mm)
Physical depth of focus (µm)
Actual fieldof view (ømm)
Weight (g) Remarks
MRL03401 CFI Plan Achromat NCG 40x 0.65 0.48 0.65 0.625 160 Spring-loaded No cover grass
MRL03901 CFI Plan Achromat NCG 100x 0.90 1.0 0.34 0.25 160 Spring-loaded No cover grass
MRD04901 CFI Plan Apochromat NCG 100x Oil 1.40 0.16 0.21 0.25 300 Spring-loaded No cover glass
24
100X / 1.30 oil Plan Fluor
DIC H ∞/ 0.17 WD 0.20
28
22.5
5
ø36f7 -0.025-0.050
ø33
Appr
ox. 7
0~17
014
8
M38×0.5
Image plane
Objective's shoulder
CFI Tube Lens UnitThis unit is attached to infinity objectives when neither a binocular or trinocular eyepiece tube is used.• Infinity Tube Lens Unit for CFI objectives (chromatic
aberration-free infinity corrected objectives).• Focal length: 200mm (7.87 in.)• To obtain the optimal objective performance, keep the
distance between the tube lens and the objective’s shoulder within 70–170 mm (2.76–6.69 in.).
CFI60 Objectives
Glossary
Numerical aperture is generally indicated by the equation below.
Numerical aperture is the most important factor in judging the objective's resolving power, brightness,
and depth of focus.
Working Distance (W.D.) and Parfocal DistaneceWorking distance is the distance between the top lens of the objective and the surface of
the specimen (or the cover glass) when the specimen is focused. The distance between the
objective's shoulder and the specimen (or the cover glass) when the specimen is focused is
referred to as parfocal distance. Nikon's CF infinity objectives have a parfocal distance of 60mm.
Objective Lens
(n=1 for air, 1.515 for oil, 1.333 for water)
Numerical aperture (NA)
Where,NA = n × sin θ n = Refractivity of the substance existing between the specimen and the objective.
sinθ = Angle that is formed by the optical axis and the light ray that passes to the extreme periphery of the objective lens.
The closest proximity of two objects that can be seen as two distinct regions of the image. Resolving power
is generally indicated by the equatuon below, where the larger the NA the greater the resolving power.
Resolving Power
Resolving Power =λ
NAWhere,λ = Light source's wavelength (generally 0.55μm)NA = Numerical aperture of objective
Specimen
0.61 ×
Working Distance
Parfocal Distance
Immersion Oil
Cover Glass
CFI Second Objective Lens UnitMXA22018
25
The range in front of and behind the target plane of the specimen, within which the observed structure can be sharply focused. The accommodation
power of the human eye varies from person to person, so does depth of focus. Depth of focus is indicated by the equation below.
Depth of Focus (When observing with eyepieces)
Depth of focus =
Physical depth of focus =
n × λ
n × λ
2 × (NA)2
2 × (NA)2
λ = Light source's wavelength (generally 0.55μm)NA = Numerical aperture of objectiveM = Total magnificationn = Refractivity of the substance existing between the
specimen and the objective. (n=1 for air, 1.515 for 0il, 1.333 for water)
+ n 7 × NA× M × 1000
The pupil diameter of the objective lens is expressed by the following equation:
Pupil Diameter
Focal length
Pupil diameter = 2 × f × NA
f =200/Mo Mo = Objective's magnification
f = Focal length of objective lensNA = Numerical aperture of objective
When viewed through eyepieces
Total Magnification
When viewed on monitors
Eyepiece observation magnification (M) = objective's magnification × eyepieces magnification
Monitor observation magnification = objective's magnification × TV adapter magnification × monitor magnificationMonitor magnification varies depending on the imaging device size of the TV camera used and the monitor size. For information, see the table below.
Imaging device size Monitor magnificationType Diagonal length Longer side Shorter side
1/3-inch 6.0mm 4.8mm 3.6mm
1/2-inch 8.0mm 6.4mm 4.8mm
2/3-inch 11.0mm 8.8mm 6.6mm
Monitor Size
Imaging device size 9-inch 14-inch 20-inch
1/3-inch 38.1× 59.2× 84.6×
1/2-inch 28.6× 44.4× 63.5×
2/3-inch 20.8× 32.3× 46.2×
26
Condensers
ø55
ø59
ø43
ø40
ø54
R400.
78
54.9
55.6
0.40.3
0.20.1
0.50.6
0.7
0.8M
AD
E IN
CH
INA
Achr N
A=0.9
C-SW
R68
35.5
70
27.5
R69
47
98.8
31.8
54.8
7.7 ø40
70˚
C-C Abbe Condenser Dry
C-SW Achromat Swing-out Condenser 1-100x
ø58
40R
ø55
ø54
854
.52
56.5
ø36.8
ø40
R42
R45
0.8 0.6 0.5 0.2 0.10.4 0.3Achr N.A=0.9
0.7C-C
ø40
824
22.2
8.5
54.7
11.
2 ø27ø36ø66
70˚
C-AR Achromat Condenser
C-SA Slide Achromat Condenser
ø27
59
63
98
ø60
ø40
55
870
˚
ø8
30˚
0.20.8 0.6 0.40.90 JAPANAchr
Achr-Apl N.A=1.41.4 1.2 1.0 0.8 0.6 0.4 0.2
(Oil)
(Slid
glas
s th
ickn
ess)
ø40ø62
70˚
9
54.9
0.6
55.5
1ø54
C-SWA Achromat Swing-out Condenser 2x-100x
C-AA Achromat /Aplanat Condenser
27
ø59
ø42
ø22
ø36.8
ø54.5
1.5
8.6
8
63.4
54.8
29.8
24.5
12
ø40
C-LAR LWD Achromat Condenser
ø39
ø40
ø56
ø34.5
55.5
550.
5(O
il)20
70˚
149.
5
81
Dark Field CondenserOil 1.43-1.20
JAPAN
(Slid
glas
s th
ickn
ess)
Darkfield Condenser Oil
C-C Phase Contrast Turret Condenser NI-CUD Universal Condenser Dry
ø35.5
ø40
ø56
55.6
54
70˚
20.6
18
Dark Field CondenserDry 0.95-0.80
JAPAN
(Slid
glas
s th
ickn
ess)
Darkfield Condenser Dry
ø30
11.5
14.9
C-CEL
C-CEL Expander Lens (for darkfield)
>A
BS
<
30.5
24.4
9
ø43
ø51
50
3363
ø113
67.8
ø169
33
ø45
ø4740
Code.No Condensers NA O.D (mm)* Magnification Weight (g)**
MBL71100 C-C Abbe Condenser, Dry Type 0.9 1.9 4–100x 90
MBL71205 C-AR Achromat Condenser 0.85 4.2 4–100x 150
MBL11300 C-SWA Achromat Swing-out Condenser (2x-100x) 0.9/0.22 1.8 2–100x 105
MBL71305 C-SW Swing-out Achromat Condenser 1x-100x 0.9/0.11 1.4 1–100x 500
MBL71505 C-SA Slide Achromat Condenser 0.9 2.2 2-100x 250
MBL71405 C-AA Achromat /Aplanat Condenser 1.4 1.6 10–100x 180
MBL16105 C-LAR LWD Achromat Condenser 0.65 10.2 4–40x 118
MBL12000 Dark Field Condenser, Oil 1.2–1.43 1.5 20–100x 163
MBL12010 Dark Field Condenser, Dry 0.8-0.95 4.0 20–40x 175
MBL73100 C-C Phase Contrast Turret Condenser 0.9 1.9 10–100x 370
MBL99005 NI-CUD Universal Condenser Dry 0.88 2.5 2–100x 800
*Object Distance **Approximately
28
Eyepieces
22.5
CFI
10x/22
ø30
ø34
ø38.
8
49-57
CFI 10x
CFIU
W10
X/25
ø30
ø34
ø38.
8
22.5
48-56
CFI UW 10x
CFI 12.5
X / 16
22.5
42–47
ø30
ø35
ø38.
8
CFI 12.5x
22.5
37–41
ø30
ø35
ø38.
8
CFI 15
X / 14.5
CFI 15x
Code.No Eyepieces Field number Weight (g)*MAK10110 CFI 10x 22 75
MAK30105 CFI UW 10x 25 100
MAK10120 CFI 12.5x 16 63
MAK10150 CFI 15x 14.5 48
*Approximately
29
Eyepiece Tubes64
79 80.9
226.7
60
100
55
C-TB Binocular Tube
(Min
. dis
tanc
e)(M
in. a
ngle
)
(Tiltin
g sec
tion)
114
142
825
82.8
51.9 85.6ø51.5
112
(0–40)
10˚
C-TE2 Ergonomic Binocular Tube
67.5
3
ø59
99.3
225
16.5
120
52
76.5
C-TF Trinocular Tube F
73.7
3
ø5999
.2
222.5
52
17.5
172
70
103.5
57.5
C-TT Trinocular Tube T
Code.No Eyepiece Tubes Field
number ObservationInclination
Interpupillaryadjustment
Intensitysplit ratio
Weight (g)*
MBB92106 C-TB Binocular Tube 22 25° 50 to 75mm 1400
MBB93800 C-TE2 Ergonomic Binocular Tube 22 10°-30° 50 to 75mm100:0 or 50:50
two-way switching2000
MBB93106 C-TF Trinocular Tube F 25 25° 50 to 75mm100:0 or 0:100
two-way switching2000
MBB93115 C-TT Trinocular Tube T 25 25° 50 to 75mm100:0, 20:80 or 0:100three-way switching
2500
*Approximately
30
C-mount screw
ø25.4ø42
ø29.
1
11.3
40.3
99.8
46.5
464
21
ø30
17.5
26
71
62
130.8
Image plane
2112
5.8
ø60
11.340.3
151.
3
4.5 46
.5
99.8
ø29.
1ø3
0
10.5
ø54
130.8
62
Image plane
C-TEP2 DSC Port for Ergonomic Binocular TubeMBB96800
C-mount camera port with built-in 0.7x relay lens.
C-TEPF2.5 DSC Port F2.5x for Ergonomic Binocular Tube MBB96810
F-mount camera port with built-in 2.5x relay lens.
Camera Ports for Ergonomic Binocular Tube
Code.No Camera Ports for Ergonomic Binocular Tube Weight (g)*MBB96800 C-TEP2 DSC Port for Ergonomic Binocular Tube 600
MBB96810 C-TEPF2.5 DSC Port F2.5x for Ergonomic Binocular Tube 500
*Approximately
31
Illumination Modules
NI-LH Precentered LamphouseMBE95205
C-HGFIB HG 100W Adapter RMBF72315
C-HGFI HG Fiber Illuminator “Intensilight”MBF72655
C-HGFIE HG Motorized Fiber Illuminator “Intensilight”MBF72665
*With the “Intensilight” C-HGFI HG Fiber Illuminator. (C-HGFIE is not equipped with a shutter opening/closing function and ND compensation dial.)
C-HGFIF 15 HG Fiber 1.5mMBF71610
C-HGFIF 30 HG Fiber 3.0mMBF71630
7695 110
32
133
115272142
2 4
17.8
130
8
110*90
258
278.
5
25.5
7
*1831
276
.513
7
ø36
ø52
ø61
83.5
ø15
ø17
ø7.5
ø5ø9
20 24
Code.No Illumination Systems Weight (g)*
MBE95205 NI-LH Precentered Lamphouse 1000
MBF72655 C-HGFI HG Fiber Illuminator “Intensilight” 6000
MBF72665 C-HGFIE HG Motorized Fiber Illuminator “Intensilight” 6000
MBF72315 C-HGFIB HG 100W Adapter R 450
MBF71610 C-HGFIF 15 HG Fiber 1.5m 100
MBF71630 C-HGFIF 30 HG Fiber 3.0m 150
*Approximately
32
Intermediate Modules67
102
8610
4.5
R70.5
362
ø40
19 80 126.5
80
5
2542
137.
3
2
12
6
Light
axis
67
294128
172
140
23
R(86)
R(8
6)
7062
.7
120
445
24.5
206.5
8084
23
CI-FL Epi-Fluorescence AttachmentMBE94310
D-FL Epi-Fluorescence AttachmentMBE74100
100
13.5
4525
5
50.5
57.522
175
ø52
ø51
285.5(Top View)
(Right-side View)
5545
1000
Y-IDP Double PortMBB74100
Y-IDP Double Port 0/100MBB74105
Y-IM Magnification ModuleMBB75500
285.5
100
455 ø51
50.5 175
50
285.5
1.6
76.523.3
33
210
191
15
58.538
3.5
ø42
11.516
15
ø51.5
77.5
5.5
66
ø42
3.5210
191
38 58.515
5.5
66
11.5
15
16
77.5
ø51.5
NI-FLT6-E Motorized Epi-fluorescence Cube Turret MBE94300
NI-FLT6 Epi-fluorescence Cube Turret MBE94500
NI-FLT6-I Intelligent Epi-fluorescence Cube Turret MBE94400
58.538
1519
1
210 3.5
ø42
77.5
ø51.5
5.5
66
21
38
60˚
35
3316.5
6.7
(24.7)
ø29.
5
16 18
30˚
C-FL BLK Blank Filter CubeMXA22030
Same as outline images of C-FL Epi-fl Filter Cubes.
34
Code.No Intermediate Tubes Weight (g)*MBE94310 CI-FL Epi-Fluorescence Attachment 2000
MBE74100 D-FL Epi-Fluorescence Attachment 3300
MBB74100 Y-IDP Double Port 1300
MBB74100 Y-IDP Double Port 0/100 1300
MBB75500 Y-IM Magnification ModuleY-IDP Double Port 1300
MBE94300 NI-FLT6-E Motorized Epi-fluorescence Cube Turret 2500
MBE94400 NI-FLT6-I Intelligent Epi-fluorescence Cube Turret 2500
MBE94500 NI-FLT6 Epi-fluorescence Cube Turret 2500
MBE94100 NI-FLEI Epi-fluorescence Attachment 1500
MBE94200 NI-BPU Back Port Unit 2500
*Approximately
NI-BPU Back Port UnitMBE94200
A
A
125 128
ø12
ø10
12
ø32
ø9
ø75
199
15
5866
6625
ø8
3
14
35
ø10
2.5
77
70
1749
4941
7010
1
80
70
80
54.8
107.
7
4-ø11
66 2.5
NI-FLEI Epi-fluorescence AttachmentMBE94100
Intermediate Modules
35
Contact Arm
NIU-CAM Contact ArmMBD92110
NI-SAM Standard ArmMBD92000
The MBD92110 NIU-CAM Contact Arm for use with Motorized Nosepieces and Intelligent Nosepieces on page 37, and the MBD92000 NI-SAM Standard Arm for use with Manual Nosepieces on the same pages.
(100
0)
(100
0)R33
.5
R80
127
53.5
319.
4
30
ø72
26
127 30
ø72
32
27
R86
.5
53.5
319.
4
107
R33.5
26
R48.5R41.5
49.2 69
.2
Code.No Contact Arm Weight (g)*MBD92110 NIU-CAM Contact Arm 1500
MBD92000 NI-SAM Standard Arm 1400
*Approximately
36
Nosepieces
485153
ø104.8
105.450
22.5
C-N6 Sextuple Nosepiece ESDMBP71316
48
ø106
105.8
53
50
22.5
C-N6A Sextuple Nosepiece with Analyzer SlotMBP71325
48
ø116
22.5
117.850
53
D-ND6 DIC Sextuple NosepieceMBP73325
37
48
ø116
ø43.5
80.7
50
22.5
117.8
53
NI-ND6-E Motorized DIC Sextuple NosepieceMBP98325
23
4
48
ø126.5
ø43.5
80.5
22.5
50
129
53
NI-N7-E Motorized Septuple NosepieceMBP98405
2
3
ø116
48
117.650
22.5
NI-ND6-I Intelligent DIC Sextuple NosepieceMBP99325
48
ø116
22.5
117.850
53
NI-N7-I Intelligent Septuple NosepieceMBP99405
Code.No Nosepiece Weight (g)*MBP71316 C-N6 Sextuple Nosepiece ESD 450
MBP71325 C-N6A Sextuple Nosepiece with Analyzer Slot 450
MBP73325 D-ND6 DIC Sextuple Nosepiece 1000
MBP98325 NI-ND6-E Motorized DIC Sextuple Nosepiece 1300
MBP98405 NI-N7-E Motorized Septuple Nosepiece 1300
MBP99325 NI-ND6-I Intelligent DIC Sextuple Nosepiece 1200
MBP99405 NI-N7-I Intelligen Septuple Nosepiece 1300
*Approximately
38
ø23.1
ø25.4
30.7
116.
8
27417
.526
ø53
ø42
Image plane
C-Mount TV Adapter 0.6xMQD42060
Camera Adapters3 9.3
ø12
7227
ø52
ø38
35
ø52
V-T Photo AdapterMAB53410
76.5
ø51
ø38
ø38
ø59
Y-TV TV TubeMBB73525
ø38 8.5
55
ø59
ø50
46.5
ø40
Y-TV 0.55x TV TubeMBB73550
C-mount screw
Image plane
ø38
ø50
41
11.4
717
.526
25.5
ø25.4
30 °
C-DA C-Mount AdapterMQD42005
ø25.4
ø50
ø40
413
.8
17.5
26
13
30.8
Image plane
C-mount screw
30.8
ø23.1ø42
84.8
4
17.5
26
ø25.4ø32
Image plane
C-Mount Adapter 0.55xMQD42055
C-Mount TV Adapter 0.35xMQD51040
ø48ø25.4
ø38
25.5
31.0
8
4 1.58
17.5
26
Image plane
ø23.1
ø25.4
30.7
100.
1
27.3
417.5
26
ø53
ø42
Image plane
C-Mount Adapter 0.7xMQD42070
C-Mount TV Adapter 0.45xMQD42040
39
ø38
68
4229
ø53
Image plane
C-Mount TV Adapter VM 2.5xMQD42120
ø38
109
83
ø53
29
Image plane
ø40 f8
ø17.3
ø60
1312
.546
.5
4.5
30
Image plane
ø43.2
ø40 f8
ø50
ø60
1373
.346
.5
4.5
10.5
90.8
Image plane
C-Mount TV Adapter VM 4xMQD42140
DS-F F-Mount Adapter MQD43000
DS-F2.5 F-Mount Adapter 2.5x MQD43020
Code.No CCTV Camera Adapters Weight (g)*MAB53410 V-T Photo Adapter 200
MBB73525 Y-TV TV Tube 350
MBB73550 Y-TV 0.55x TV Tube 300
MQD42005 C-DA C-Mount Adapter 180
MQD42055 C-Mount Adapter 0.55x 300
MQD42070 C-Mount Adapter 0.7x 155
MQD51040 C-Mount TV Adapter 0.35x 300
MQD42040 C-Mount TV Adapter 0.45x 620
MQD42060 C-Mount TV Adapter 0.6x 650
MQD42120 C-Mount TV Adapter VM 2.5x 165
MQD42140 C-Mount TV Adapter VM 4x 338
MQD43000 DS-F Mount Adapter 80
MQD43020 DS-F2.5 F-Mount Adapter 2.5x 200
*Approximately
40
Main Body and Accessories
148.5
320
140
315
4
R800 R800R1500
815
315
34
ø48
143
R450
R1400
68
133
269
383
341.7
POWER
CAPTURE F.S.
ND
OUT
IN
8NCB11
ND32
Ni-U Main BodyMBA92010
(-1.
0 ~
36.
0)
2547 53
.5
22 13.6 147.8
11.3
23.5
459
76
R38
ø10
84 151
277.771.5
ø26.2
NI-CH Condenser HolderMBD94000
72.5
196
94
84120
20.5
91.5
7.5
NI-SS SubstageMBD93000
To mount the condensers in this brochure on the Ni-U main body, the following holder and substage are required.
41
220
93
160
6
NI-CTLB Control Box BMBF95410
EPI FL INTSL
SHUTTER
12481632
INTSL ND
OBJ FL
CONTROL
NI-SRCPMADE IN JAPAN
DIA
OPEN / CLOSE
120.
5
2.2 20 76
1750
37
3010
NI-SRCP Simple Remote Control PadMBF95120
The control box and control pad below are required to use the Ni motorized unit and intelligent unit via the NIU-CAM Contact Arm (page 35).
Code.No Illumination Systems Weight (g)*MBD94000 NI-CH Condenser Holder 350
MBD93000 NI-SS Substage 800
MBA92010 NI-U Main body 11000
MBF95410 NI-CTLB Control Box B 2500
MBF95120 NI-SRCP Simple Remote Control Pad 200
*Approximately
En(1609)T Code No.2CE-MUZH-4 This brochure is printed on recycled paper made from 40% used material.
Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. September 2016 ©1997-16 NIKON CORPORATION
N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure shall be required in case of export from Japan.*Products: Hardware and its technical information (including software)
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