Post on 17-Jul-2020
transcript
Molecular response of Deinococcus radiodurans to simulated microgravity explored by
proteometabolomic approach
Emanuel Ott, Felix M. Fuchs, Ralf Moeller, Ruth Hemmersbach, Yuko Kawaguchi,
Akihiko Yamagishi, Wolfram Weckwerth and Tetyana Milojevic
*Corresponding author
tetyana.milojevic@univie.ac.at
Supplementary Information:
Supplementary Figure 1: Heatmap of normalized LFQ intensities from significantly different
expressed proteins identified in every replicate.
Supplementary Figure 2: Results of protein and peptide quantification photometric assays for
sample normalization.
Supplementary Table 1: Raw protein LFQ intensities, corresponding statistical analysis,
number of identified peptides and calculated Maxquant score.
Supplementary Table 2: Targeted metabolomics approach with normalized peak areas and
corresponding statistical analysis.
ctrl1
ctrl2
ctrl3
ctrl4
ctrl5 ug
1
ug2
ug3
ug4
ug5
Q9RSL0Q9RRA3Q9RS87Q9RV30Q9RR73Q9RZ77Q9RYY9Q9RUD0Q9RT26Q9RSQ6Q9RRT6Q9RR70Q9RSA6Q9RT62Q9RVJ0Q9RY49Q9RUT7Q9RTQ7Q9RWH6Q9RWC1Q9RUL7Q9RX33Q9RVJ3Q9RT72Q9RUY2Q9RXV6Q9RXK6Q9RSC5Q9RTT6Q9RVG1Q9RUB0Q9RRV8Q9RV18Q9RWB0Q9RU57Q9RUW4Q9RT42Q9RWN5Q9RTQ6Q9RT60Q9RZ07Q9RV70Q9RTA9Q9RRM6Q9RZT2Q9RTQ0Q9RXD5Q9RXT4Q9RUZ9Q9RTM4Q9RZL4Q9RYN6Q9RZD1Q9RRX5Q9RUG2Q9RRK0Q9RSV3Q9RV84Q9RXY9Q9RW95Q9RVP1Q9RR87Q9RZE6Q9RZ24Q9RRE5Q9RT44Q9RZE9Q9RWP9Q9RXL1Q9RYH8Q9RS86Q9RVU2Q9RRR7Q9RRW1Q9RSQ5Q9RSD0Q9RSJ4Q9RWN6Q9RS28Q9RTN5Q9RYS7Q9RX25Q9RRV3Q9RYL8Q9RTY4Q9RXQ3Q9RZT0Q9RX54Q9RVW8Q9RXW4Q9RZI5Q9RZ06Q9RT05Q9RTF5Q9RTK2Q9RWB9Q9RXG1Q9RZ11Q9RTD1Q9RUD9P52027
Q9RW85Q9RX89Q9RUE0Q9RT36Q9RWU2Q9RX52Q9RV56Q9RTZ4Q9RU62Q9RZ99Q9RRS5Q9RXB0Q9RWI2Q9RZS5Q9RWI3Q9RWI0Q9RUC8Q9RZS7
−2 0 2Row Z−Score
Figure S1: Heatmap of normalized (z-scored) LFQ values of significantly different expressed proteins identified in every replicate. The minimum requirement for an identification was one unique peptide.
Figure S2 A) Protein concentration in µg/µL of each replicate (1:5 diluted) against a BSA
standard in different concentrations. B) Peptide concentration in ng/µL of each replicate,
measured against a standard peptide digest solution.