Nasonia vitripenniNasonia vitripenniYou are the You are the PIPI ( (PPrimary rimary IInvestigator)nvestigator)

Jewel WaspJewel WaspTell me about it…Tell me about it…

Life CyclesLife Cycles

Haplo/Diplo GeneticsHaplo/Diplo Genetics

Wasp Development Wasp Development TimelinesTimelines

1818oo, 28, 28o o CC

ComplementationComplementation

Process by which an allele of each Process by which an allele of each of two mutant genes makes up for of two mutant genes makes up for

the defect in the other gene.the defect in the other gene.

You Find Three Mutants With a You Find Three Mutants With a Similar Phenotype Similar Phenotype

Are they the Same Genotype?

Possible PhysiologyPossible Physiology

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

transport

ComplementComplement

a

|

|

B

a

|

|

B

A

|

|

b

A

|

|

b

x

A

|

|

b

a

|

|

B

Two genes

Both Recessive

Think about dominant mutations!

Fail to ComplementFail to Complement

A

|

|

b

A

|

|

b

A

|

|

b

A

|

|

b

x

A

|

|

b

A

|

|

b

1 gene

Three scarlet eyed mutants, how many Three scarlet eyed mutants, how many loci?loci?

ststDRDR,, ststCC,, stst318318

Connect same gene with a line.

stDR

stC

st318

Fail to complem

ent

For example.

HandlinHandling Waspsg Wasps

Sexing WaspsSexing Waspsadultsadults

Sexing WaspsSexing Waspslarvae, tuxedo – black stageslarvae, tuxedo – black stages

Sex PicturesSex Pictures

Scoring Red EyesScoring Red Eyes

Here

Scoring Eye ColorScoring Eye Color

Another Chart, Same Another Chart, Same InfoInfo

Wasp BusinessWasp Business

Link

To DoTo DoFridayFriday

To Do

Friday -Wednesday?…up to you!

Plan Your Plan Your CrossesCrosses

Think about the wasp’s Think about the wasp’s schedule, schedule,

Think about your Think about your schedule,schedule,

Plan accordingly!Plan accordingly!

ststDRDR,, ststCC,, stst318318

F1 data, F2 data that means somethingF1 data, F2 data that means something

April 14 - 23April 14 - 23

Finish Finish C. elegans C. elegans experiment,experiment,

Tinker with wasps,Tinker with wasps,

Exam ReviewExam Review (April 20 (April 20thth))

Exam I (April 23Exam I (April 23rdrd),), Basic Mendel!Basic Mendel! Basic Mendel!Basic Mendel! Basic Mendel!Basic Mendel! Basic Mendel!Basic Mendel! Basic Mendel!Basic Mendel! C. elegansC. elegans!! Bioinfomatics!Bioinfomatics! Nasonia!Nasonia!

No Lecture April 13No Lecture April 13thth