Post on 09-Jun-2018
Preview:
transcript
PacBio
2003
Levene et al. 2003
Korlach et al. 2008
Eid et al. 2009
Flushberg et al. 2010
Stats
‣ Mean RL ~ 3,000 bp ‣ 95th percentile ~ 8,000 bp ‣ Max RL ~15,000 bp ‣ 30,000 - 50,000 reads ‣ 100 - 150 Mb/ SMRT cell ‣ Runtime 90 min
Types of runs
‣ CLR: Continuous Long Reads ‣ CCS: Circular Consensus Sequencing
Error profile and GC bias
Carneiro et al. 2012
Loading regimes
‣ Diffusion loading ‣ Magnetic bead loading
Modification detection
Flusberg et al. 2010
Error correction
Koren et al. 2012
Closing gaps
Chaisson et al. 2015
Transcript sequencing
Tilgner et al. 2014
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