Purpose & Objectives

ROL ♜ S p o r o s a r c i n a p a s t e u r i i  

♜ Cult ivat ion v 3 0 ° C S h a k i n g t a b l e v 2 4 h v à petr i d ish

♜ R e p e a t e d i n v a t

♜ S o l i d m e d i a  v Y e a s t : 2 . 0 0 g v Ammonium sul fate ���

(NH4) 2 SO 4 : 1 .00 g v Tr is ���

(HOCH 2 ) 3 (CNH 2 ) : ���1 . 5 7 g

v Agar : 2 .00 g

♜ Liquid media v Y e a s t : 2 0 . 0 0 g i n 4 0 0

m L v Ammonium sul fate : ���

1 0 . 0 0 g i n 3 0 0 m L v 5 . 7 3 g o f t r i s in ���

3 0 0 m L

♜ Derived f rom ECC R0 ���(L i , 2004)  

♜ Changes to Formula: v Less superplast ic izer v W/C = 0 .395

♜ Amounts v Cement : 446.39 g

♜ F 1 1 0 s i l i c a s a n d :4 4 6 . 0 5 g

♜ Polyvinyl a lcohol (PVA) f ibers : 11 .90 g

♜ Water/medium:1 7 8 . 9 0 g ( f o r E C C : 1 . 6 g o f superplast ic izer )

Bacteria Culturing

Nutrient Mediums

Mix

Cubes: 5.08 x ���5.08 x ���5.08 cm ���

Beams: 12.7 x ���2.54 x 2.54 cm ���

(Note that each beam was in i t ia l ly cast in a

3 0 . 4 c m m o l d s b u t w a s b i s e c t e d a f t e r o n e w e e k )

1: Laboratory v  Control v  Lime water v  3 0 ° C

3: Underground v  Buried 21.6 cm

2: Exposed v  Dry, l i t t le ra in (Angel , 2013)

0%   2%   4%   6%   8%   10%   12%   14%  

Control:  ECC  

Control:  ECC  +  Medium  

Control:  ECC  +  Bacteria  

Exposed:  ECC  

Exposed:  ECC  +  Medium  

Exposed:  ECC  +  Bacteria  

Undergound:  ECC  

Undergound:  ECC  +  Medium  

Undergound:  ECC  +  Bacteria  

R.F. % Increase

Envi

ron

men

tal G

rou

p: T

ype

Self-Healing in all Groups

-­‐5.00%  

0.00%  

5.00%  

10.00%  

15.00%  

20.00%  

0.00%   5.00%   10.00%   15.00%   20.00%   25.00%  Perc

ent

incr

ease

in R

.F. a

fter

rec

ove

ry

Percent reduction in R.F. from damage

Damage  Healing  Correla.on  

Laboratory  Environment   Underground  Environment  

Exposed  Environment   Linear  (Laboratory  Environment)  

Linear  (Underground  Environment)   Linear  (Exposed  Environment)  

0%  

2%  

4%  

6%  

8%  

10%  

12%  

ECC   ECC+  Medium   ECC  +  Bacteria  

R.F

. % in

crea

se

Type

Healing amongst ECC Types

0%  

2%  

4%  

6%  

8%  

10%  

12%  

14%  

Control   Exposed   Underground  

R.F

% in

crea

se

Environment

Healing by Environment

0.00  0.10  0.20  0.30  0.40  0.50  0.60  0.70  0.80  

OPC

-­‐R  

OPC

-­‐M  

OPC

-­‐B  

ECC-­‐R  

ECC-­‐M  

ECC-­‐B  

Exponential Rate of Water

Uptake

0  

10000  

20000  

30000  

40000  

50000  

60000  

70000  

Pasc

als

req

uir

ed fo

r Fa

ilu

re

Individual ECC specimen ECC type average

Compressive Strength

0.00%  

2.00%  

4.00%  

6.00%  

8.00%  

10.00%  

12.00%  

14.00%  

0   10   20   30   40   50   60  

Mas

s u

pta

ke b

y %

Time [hrs]

Sorptivity

OPC-­‐R  

OPC-­‐M  

OPC-­‐B  

ECC-­‐R  

ECC-­‐M  

ECC-­‐B  

Natural Self-Healing

Bacterial Concrete

ECC

ECC in the Field

Specimen Preparation

Environments

RF Testing

Comp. & Sorpt. Testing + Stats

General Trends

Type Healing

Field Healing

Comp. & Sorpt. Results

Hypothesis My Role Conclusions

Methods Results

I would l ike to thank my mentor , Dr . Paramita M o n d a l , he r do cto r al cand idat es , Pet e S t y n o s k i and Bin Zh an g al ong with gra duate s t udent J e e v a k a S o m a r a t n a, my sc ience research advisors : Mr . David Keith , Mr . Ken Kaplan, and Ms. Stephanie Greenwald, my parents : Dr . Howard Kap lan an d M s. J e nni fer L acks Kapl a n, my s te p m other : J anet S h i m e r, and last l y m y grandfather : Dr . Sanford Lac k s .

Acknowledgements