Post on 26-Jul-2018
transcript
PPSQ-31A/33A
ShimadzuProtein Sequencer
C297-E055A
Printed in Japan 3295-12801-10ANS
The contents of this brochure are subject to change without notice.
JQA-0376
SHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.com
Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at www.shimadzu.com
Achieving Greater Simpl ic i ty and Rel iabi l i ty in the Determinat ion of Amino Acid Sequences
Shimadzu Protein Sequencer
PPSQ-31A/33A
A c h i e v i n g G r e a t e r S i m p l i c i t y a n d R e l i a b i l i t y i n
t h e D e t e r m i n a t i o n o f A m i n o A c i d S e q u e n c e s
P P S Q - 3 1 A / 3 3 A
Analytical Stabil i ty
Features
P 04 - Analytical Stability
P 05 -
P 06 - Contents
Excel lent basel ine stabi l i ty and retent ion t ime reproducibi l i ty
Simple operat ions and easy-to-use data analysis funct ions
Repeated Use of Mobile Phase
Software with Excellent Operabil i ty
Reduced running costs and l iquid waste in the analysis of PTH-amino acids using isocrat ic mode
Software with Excellent Operability
Repeated Use of Mobile Phase
P 07 - Specifications
Achieving Greater Simpl ic i ty and Rel iabi l i ty in the Determinat ion of Amino Acid Sequences
Shimadzu Protein Sequencer
PPSQ-31A/33A
A c h i e v i n g G r e a t e r S i m p l i c i t y a n d R e l i a b i l i t y i n
t h e D e t e r m i n a t i o n o f A m i n o A c i d S e q u e n c e s
P P S Q - 3 1 A / 3 3 A
Analytical Stabil i ty
Features
P 04 - Analytical Stability
P 05 -
P 06 - Contents
Excel lent basel ine stabi l i ty and retent ion t ime reproducibi l i ty
Simple operat ions and easy-to-use data analysis funct ions
Repeated Use of Mobile Phase
Software with Excellent Operabil i ty
Reduced running costs and l iquid waste in the analysis of PTH-amino acids using isocrat ic mode
Software with Excellent Operability
Repeated Use of Mobile Phase
P 07 - Specifications
4
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
mV
D
E
TH
N Q S
G AY R
M VP W
F
K
I L
PTH-AA
2D Electrophoresis Image of Proteins Extracted from E. Coli
10pmol
Spot 1
Sequence analysis can be started by simply registering the number of analysis cycles, data file names, and other information, and then clicking the [Start] button.
When a reagent is replaced, the inside of the reagent bottle is purged with nitrogen. The replacement dates and times of the reagents can be ascertained with a glance, and lot control is possible.
5
Stable Analysis, Low Running Cost, Excellent Operabil i ty
Analytical Stabil i ty
Baseline Stabil i ty
Repeated Use of Mobile Phase
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Software with Excellent Operabil i ty
Simple Operations
Main Window
Sequence Schedule Window Reagent Control Window
Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Retention Time Reproducibil i ty
Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
cycle 1 cycle 2
cycle 14 cycle 15
cycle 24 cycle 25
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
DTT
S
DMPTU DPTU
DPU DTT
T
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
DMPTU
LDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
Y
DTT DPTU
DMPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
EDTT
DMPTUDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50mV
K
DTT
DMPTUDPTU
Selection of sequencing schedule
Summary display of currently executed cycle and step and estimated completion time
4
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
mV
D
E
TH
N Q S
G AY R
M VP W
F
K
I L
PTH-AA
2D Electrophoresis Image of Proteins Extracted from E. Coli
10pmol
Spot 1
Sequence analysis can be started by simply registering the number of analysis cycles, data file names, and other information, and then clicking the [Start] button.
When a reagent is replaced, the inside of the reagent bottle is purged with nitrogen. The replacement dates and times of the reagents can be ascertained with a glance, and lot control is possible.
5
Stable Analysis, Low Running Cost, Excellent Operabil i ty
Analytical Stabil i ty
Baseline Stabil i ty
Repeated Use of Mobile Phase
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Software with Excellent Operabil i ty
Simple Operations
Main Window
Sequence Schedule Window Reagent Control Window
Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Retention Time Reproducibil i ty
Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
cycle 1 cycle 2
cycle 14 cycle 15
cycle 24 cycle 25
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
DTT
S
DMPTU DPTU
DPU DTT
T
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
DMPTU
LDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
Y
DTT DPTU
DMPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
EDTT
DMPTUDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50mV
K
DTT
DMPTUDPTU
Selection of sequencing schedule
Summary display of currently executed cycle and step and estimated completion time
6 7
Software with Excellent Operabil i ty / Specif ications
Simple, Easy-to-Use Data Analysis FunctionsSpecif icationsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the
overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.
Reprocessing of chromatograms required
for data analysisOverlay of multiple
chromatograms
Automatic Sequence Estimation
Automatic estimation of amino acid sequences
Chromatogram peak integration, printing, and other processes can be performed on a per-sample basis.
Displays multiple chromatograms. Analysis cycles can be updated simply by clicking a button.Window allows easy identification of sequences.
Calibrates PTH-amino acids.Retention times can be edited simply by selecting a PTH-amino acid and clicking a peak.
Automatically generates a report containing analytical parameters, estimated sequences, yield rates, and other information.
If the appropriate settings are entered before analysis starts, sequences are automatically estimated after each cycle is complete. Up to four candidate amino acids are displayed together with their certainty levels.
Customized Repor ts
Reports containing information such as analytical parameters, estimated sequences, and yield rates are automatically created.
Yield Graph Display
Initial and repeated yield rates are calculated and displayed in graph form. Amino acids used for calculations can be freely selected.
Reaction method
Reaction time
Number of reactors
Sample immobilization method
Reactor temperature control
Converter temperature control
Column temperature control
Number of reagents/solvents
Reagent/solvent delivery method
Dimensions
Weight
Edman degradation
PPSQ-31A: 47 min/cycle
PPSQ-33A: 48.5 min/cycle
PPSQ-31A: 1
PPSQ-33A: 3
Glass fiber disk (8 mm dia.) or PVDF membrane
{Room temperature + 10 °C} to 60 °C
{Room temperature + 10 °C} to 70 °C
{Room temperature + 10 °C} to 60 °C
8 (one is an option)
Nitrogen gas pressure
650 (W) x 520 (H) x 500 (D) mm
PPSQ-31A: 55 kg
Main Unit
Reagents for amino acid sequence analysis (7 types)
Glass fiber disks
Polybrene reagent
Apomyoglobin standard
Mobile phase for PTH-amino acids
Separation column for PTH-amino acids
Mixed standards for PTH-amino acids
1 of each type
50
1
1
1 L
1
2 mL x 4
Part name QuantityPPSQ-31A/33A
Installation space
Power supply
Nitrogen gas
Exhaust equipment
Desktop: 1,730 (W) x 600 (D) x 550 (H) mm min.
(Weight: Approx. 93 kg)
100 VAC, 1,100 VA, 50/60 Hz
Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing
(10 m) are provided as standard accessories.
In order to provide an exhaust for gases
produced by waste liquids, an exhaust tube
must either be connected to exhaust
equipment or led outside. An exhaust tube
(20 m) is provided as a standard accessory.
Flow rate setting range
Dimensions
Weight
0.001 to 10.000 mL/min
260 (W) x 140 (H) x 420 (D) mm
11 kg
Solvent Delivery Module LC-20AT
292-31310-XX
292-05684-92
PPSQ-31A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
Wavelength setting range
Dimensions
Weight
190 to 700 nm
260 (W) x 140 (H) x 420 (D) mm
13 kg
Detector SPD-20A
Computer
Display
Note: In the interests of product improvement, these specifications may change without notice.Note: Microsoft is a registered trademark of Microsoft Corporation in the U.S. and other countries.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.
Note: The required installation space and power supply may change according to the PC and display used.
Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.
Offers highly cost-effective performance.
PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)
IBM PC/AT or compatible
OS: Microsoft® Windows® XP
17-inch LCD
Control PC
Standard Configurations
Star tup Kit
Other I tems Provided by User
PPSQ-31A System
292-31330-XX
292-05684-92
PPSQ-33A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
PPSQ-33A System
PPSQ-31A Single Reactor
6 7
Software with Excellent Operabil i ty / Specif ications
Simple, Easy-to-Use Data Analysis FunctionsSpecif icationsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the
overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.
Reprocessing of chromatograms required
for data analysisOverlay of multiple
chromatograms
Automatic Sequence Estimation
Automatic estimation of amino acid sequences
Chromatogram peak integration, printing, and other processes can be performed on a per-sample basis.
Displays multiple chromatograms. Analysis cycles can be updated simply by clicking a button.Window allows easy identification of sequences.
Calibrates PTH-amino acids.Retention times can be edited simply by selecting a PTH-amino acid and clicking a peak.
Automatically generates a report containing analytical parameters, estimated sequences, yield rates, and other information.
If the appropriate settings are entered before analysis starts, sequences are automatically estimated after each cycle is complete. Up to four candidate amino acids are displayed together with their certainty levels.
Customized Repor ts
Reports containing information such as analytical parameters, estimated sequences, and yield rates are automatically created.
Yield Graph Display
Initial and repeated yield rates are calculated and displayed in graph form. Amino acids used for calculations can be freely selected.
Reaction method
Reaction time
Number of reactors
Sample immobilization method
Reactor temperature control
Converter temperature control
Column temperature control
Number of reagents/solvents
Reagent/solvent delivery method
Dimensions
Weight
Edman degradation
PPSQ-31A: 47 min/cycle
PPSQ-33A: 48.5 min/cycle
PPSQ-31A: 1
PPSQ-33A: 3
Glass fiber disk (8 mm dia.) or PVDF membrane
{Room temperature + 10 °C} to 60 °C
{Room temperature + 10 °C} to 70 °C
{Room temperature + 10 °C} to 60 °C
8 (one is an option)
Nitrogen gas pressure
650 (W) x 520 (H) x 500 (D) mm
PPSQ-31A: 55 kg
Main Unit
Reagents for amino acid sequence analysis (7 types)
Glass fiber disks
Polybrene reagent
Apomyoglobin standard
Mobile phase for PTH-amino acids
Separation column for PTH-amino acids
Mixed standards for PTH-amino acids
1 of each type
50
1
1
1 L
1
2 mL x 4
Part name QuantityPPSQ-31A/33A
Installation space
Power supply
Nitrogen gas
Exhaust equipment
Desktop: 1,730 (W) x 600 (D) x 550 (H) mm min.
(Weight: Approx. 93 kg)
100 VAC, 1,100 VA, 50/60 Hz
Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing
(10 m) are provided as standard accessories.
In order to provide an exhaust for gases
produced by waste liquids, an exhaust tube
must either be connected to exhaust
equipment or led outside. An exhaust tube
(20 m) is provided as a standard accessory.
Flow rate setting range
Dimensions
Weight
0.001 to 10.000 mL/min
260 (W) x 140 (H) x 420 (D) mm
11 kg
Solvent Delivery Module LC-20AT
292-31310-XX
292-05684-92
PPSQ-31A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
Wavelength setting range
Dimensions
Weight
190 to 700 nm
260 (W) x 140 (H) x 420 (D) mm
13 kg
Detector SPD-20A
Computer
Display
Note: In the interests of product improvement, these specifications may change without notice.Note: Microsoft is a registered trademark of Microsoft Corporation in the U.S. and other countries.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.
Note: The required installation space and power supply may change according to the PC and display used.
Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.
Offers highly cost-effective performance.
PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)
IBM PC/AT or compatible
OS: Microsoft® Windows® XP
17-inch LCD
Control PC
Standard Configurations
Star tup Kit
Other I tems Provided by User
PPSQ-31A System
292-31330-XX
292-05684-92
PPSQ-33A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
PPSQ-33A System
PPSQ-31A Single Reactor
PPSQ-31A/33A
ShimadzuProtein Sequencer
C297-E055A
Printed in Japan 3295-12801-10ANS
The contents of this brochure are subject to change without notice.
JQA-0376
SHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.com
Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at www.shimadzu.com