Post on 24-Jun-2015
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Preparation and Characterization of Cellulose
Fibers for Biochemical Applications
Delaine Zayaz-Bazán1, José A. Cruz-Arzón1, Nelson Granda-Paz1, and Wilfredo Otaño-Rivera3,4
1-Department of Biology, University of Puerto Rico at Cayey, Puerto Rico1-Department of Chemistry, University of Puerto Rico at Mayagüez, Puerto Rico
3- Department of Physics-Mathematics, University of Puerto Rico at Cayey, Puerto Rico
4-Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico
Problem•Is it possible to prepare electrospun
cellulose fibers that could be used for separation of proteins producing high flow and mass transfer?
Objectives• Prepare cellulose acetate fibers by
electrospinning and transform them into cellulose by a deacetylation process
• Characterize fibers by SEM and FTIR among other techniques.
• Evaluate chemical and physical electrospinning process parameters in order to craft porosity of cellulose fibers.
Basic ConceptsCellulose Acetate Cellulose
• Molar Mass 30,000
• Density 1.3 g/cm3
• Molar Mass 600,000
• Density 1.5 g/cm3
•Cellulose Acetate for fiber synthesis
•Cellulose for protein separation
Methodology
Methodology
+
Cellulose acetate Acetone/DMF/EtOH
Cellulose 21% sol
Electrospinning
• Typical conditions▫ Distance: 18 cm▫ 20.0 kV▫ 0.5 mL/ hr
Results
21% CA fibers
Results
Fiber Diameter
Average 693 ±306
Deacetylation
Regenerated Cellulose Membrane
OH-
500100015002000250030003500400045005000
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
AC.jws.dx
Wavenumbers [1/cm]
Tran
smitt
ance
1745
FTIR Analysis
1740
FTIR Analysis
Deacetylation
•Cellulose Acetate
•Cellulose
Esther Functional Group
Hydroxyl Group
0500100015002000250030003500400045005000
-0.02
0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
control #2 C Vibha Smooth.jws.dx smoothed smoothedm#D CR.jws.dx smoothed smoothed
Wavenumbers [1/cm]
Tra
nsm
itta
nce
1640
FTIR Analysis
Regenerated vs. Comercial Cellulose
Porosity Measurements
±9
Conclusions
•The work is still going on ▫The preliminary results show that cellulose
can be regenerated from the acetate•Future Works
Acknowledgements
•UPR at Cayey
•RISE Program
•NAMAS Lab
•Our Lab Group
•Professor Vibha Bansal
Thank You
Questions?