ProStar 335 Detector

Petro van Poppel – Field Support Specialist Friday, 21 April 2023

Overview

Introduction to PS 335 Detector

• Dual Array option• Poly Verify Software• Flow cell• CommunicationsPoly Verify

• Production Tests with 0x0 flow cell• Wavescan• (Settings Tab)• (Input/Output Tab)• (EEPROM)• (COMMS Tab)• (Calibration Tab)PS 335 Shipping Test

Problems

ProStar 335 Mechanical Optical layout

ProStar 335 Ray Trace Layout

335 Optics

335 Ray Trace

335 Single and Dual Array

Single Array

1024 photodiode sample array

2 photodiode references (250nm and 500 nm)

No extended range spectra

Uses only 9x0 and 4x0 flow cells

Microbore to Analytical applications

Dual Array

1024 photodiode sample and reference array

Extended range spectra (up to 70-100 AU)

Used for analytical to prep apps

Flow Cell Ray Beam

General design of the 9x0 flow cell General design of the 9x1 flow cell

Flow Cell Specs

Flow Cell Prep

(9 x 1)

Super Prep

(4 x 0.15)

Analytical

(9 x 0)

Micro

(4 x 0 )

Pressure rating

1000 psi 1000 psi 1000 psi 1000 psi

Flow Cell Volume

30 µl 145 µl 15 µl 1.5 µl

Material (non optical)

Ti & PEEK Ti & PEEK Ti & PEEK Ti & PEEK

Noise Performance

200 ml/min

10-500ml

1x10-4AU

800 ml/min

0.80-10 L

1x10-4AU

40 ml/min

0.1 µl-100 ml

5x10-3AU

1 ml/min

0-20 ml

7.5x10-3AU

Communications

TCP/IP communications for Detector:

•Static or Dynamic

•Networked or standalone

•Firewall

•Access on Network, OS or PC

PS 335 PolyVerify Software

PS 335 PolyDiagnostics

Ctrl-Shift-F10 to enable Diagnostics sideCtrl-Shift-F9 to hide Diagnostics side

335 PolyVerify Test Certificate

Test Certificate provided by the factory for each 335 detector

PS 335 PolyVerify Tests

PS 335 Noise Test

PS 335 Wavelength Accuracy test

Run with a 4 nm slit

PS 335 SBW Accuracy Test

Wavelength Range of 190 to 950 nm

PS 335 Detector Linearity Test

pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.

PS 335 Wavescan

PS 335 Wavescan Results

PS 335 Contributions from each Lamp

PS 335 PolyDiagnostics Mode

PS 335 Input/Output Mode

PS 335 Main PWB Power Supply LED’s

A: + 24 V power SupplyB: + 9V and – 9V Power SupplyC: + 5 V Power Supply

BA

C

Front of the Unit

PS 335 Identifying the Array Type

Knowing the Array type is necessary when running

the Input/Output test diagnostics

PS 335 Dual Array P8 disconnect

P8 connection

PS 335 PolyDiagnostics EEPROM

PS 335 PolyDiagnostics COMMS

PS 335 PolyDiagnostics Calibration Tab

Caffeine Shipping Test

PS 335 Caffeine Shipping Test: Test made up of 1 components:

• Loop injection using 25 µg/ml caffeine

Test is conducted using Galaxie Software

PS 325/335 Caffeine Loop Injection Test

Test Calculates:Peak HeightPeak AreaNoiseS/N

Common Problems

•High Noise

•Lamp on, not ready

•SIG_CC_SLIT_PEAK_NOT_FOUND

•SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL

•COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED

•9555 LMP_CC_D2_FILAMENT_FAILURE

•Foggy Optics

PS 335 Error Messages Table can be found:

• PS 335 Service Manual

• Dealernet

Foggy Optics

What is Foggy Optics?

What is Foggy Optics?

Three types of Foggy optics

• White powdery coating

• Rust/brown spot burn

• Speckled mirror (delamination)

Foggy Optics

What is Foggy Optics?

Diagnosing Foggy Optics

Diagnosing Foggy Optics

Run a “wavescan” test,

When bad

Open the optics and inspect.

Wavescan test results

Intensity for the 254 nm lineCurrently is 10 – 50 K counts

False positive

New D2 vs Aged D2 Intensities

Foggy Optics

What is Foggy Optics?

Diagnosing Foggy Optics

Treating Foggy optics

0603 Project (Foggy Optics)

Proposed Fixes and their status:

White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors

New Flow cells & fittings available for all cells except 4 x 0.15

Brown powder fogging: due to UV attack on some of the foam parts close to the UV source.

Eliminating as much of the foam as possible and shielding the rest.

Mirror delamination: due to UV attack on the thin Al coating of the mirrors

Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.

Opening and cleaning the optics

Cleaning 325 optics

Clean area in circle

Do not adjust!!

Replacing Optics

Wavelength calibration data must be entered into the 335 EEPROM

Replacing Optics

This label is attached to the top of each optics module, the shipping box and the

test document

Summary

•Single and Dual Array

•Optical path is open

•Flow cell is dual path

•Diagnostics Tests, like wavescan

•Shipping Test

•Main Problems (Foggy Optics)