• Established in 1982, Randox employs over 1000 people

globally

• ISO 13485 Accredited manufacturer

• Distribution agreements in 130 countries

• 30% gross income reinvested into R&D

• Clinical Diagnostics, Quality Assurance programmes and

Drug Residue Testing Kits

• Over 20 years experience in developing ELISA based

technology

Revolutionising healthcare through

continuously

improving diagnostic solutions”“

About Randox

About Food

Diagnostics Improving food diagnostic industry testing by reducing costs, analysis time and labour requirements while increasing productivity output

To increase consumer confidence by improving food safety

Cost effective food diagnostic Cost effective food diagnostic

testingtesting

Honey

Placements

Current

Placements 1. Famille Michaud (France)

2. Langnese (Germany)

3. Sadecki Bartnik (Poland)

4. Bartnik LLC (Ukraine)

5. Citrofrut (Mexico)

6. Little Bee Impex (India)

7. Geomiel (Argentina)

8. Capilano (Australia)

9. Ministry of Health Cape Town (South

Africa)

10. Agro East Trade (Ukraine)

Current

Placements

11 Anses EU CRL (France)

12 Medas SRO (Slovakia)

13 Istituto Zooprofilattico Brescia  (Italy)

14 Grigard (Bulgaria)

15 Apis India (India)

16 Shakti Impex Honey Co. (India)

17 Pioneer Honey (India)

18 Brij Healthcare (India)

19 SC Vladovlad SRL (Moldova)

20 Biomieles (Mexico)

21 Norevo (Mexico)

22 PE Jesa (Ukraine)

23 SQTS (Switzerland)

24 Apicultura (Bulgaria)

25 Agro East Trade (Ukraine)

26 Promiel (Argentina)

27 Carmencita (Chile)

Food Screening

Solutions

Evidence Investigator and Biochip

1

2

ELISA

Principle of ELISA technology

•The antibody is attached to the surface of the microtitre plate

•Sample and conjugate (Enzyme labelled antigen) are added simultaneously

•These compete for free binding sites on the capture antibody

•The quantity of enzyme labeled antigen bound will determine the resulting colour produced

•Optical density measures the amount of light absorbed within the solution to give a concentration of residue

ELISA Kit • A single sample preparation can be

applied across various kits

• Generic antibody extends applicability

• Short incubation time – allows for fast

results

• Microtitre plates are pre-coated with

antibodies

• Excellent sensitivity and cross-reactivity

Antibiotic Validated for Honey?

Chloramphenicol Yes

Flumequine Yes

Quinolones In Development

Streptomycin Yes

Sulphamethoxazole Yes

Tetracyclines Yes

ELISA Kits for Use in Honey

Biochip Array

Technology

A Multi-Residue Testing Platform

• Simultaneous Quantitative Detection

• One single sample

• Competitive ELISA Principles

• Efficient, accurate and cost effective

Typically a series of related residues are selected & grouped in

an array format

Potential for 22 targets present on the Biochip allowing for large

scale screening

Revolutionary

Screening

Technology

The Evidence Investigator offers revolutionary screening

technology to honey laboratories:

• Quick Time Frame• Results obtained within just a few hours

• Cost Saving Element • Compared to high ELISA volumes or analytical

methods

• Laboratory Efficiency Improved• Results are multiplexed and significant time saving

benefits are gained

The Complete

Honey Screening

Package PC & Imaging

SoftwareBarcodeScanner

Thermoshaker

Biochip Holding

Rack

Investigator Imaging

Module (Bench Top System)

Additional

Support

Covered in

System Package

• Randox will provide on-site training from a HQ based

Installation Scientist (built into quotation)

• Normally this will be a 3 – 5 day programme depending

on what Arrays are required at the site

• Full 1 year warranty and 1 year 24 hour support if

required

• Online troubleshooting also available remotely

• Training provided on all aspects of sample preparation,

assaying technique and results interpretation

Sample

Preparation of

AM IA simple dilution is completed using provided aqueous buffers such as diluted wash buffer

1.Weigh out 1g of honey

2.Add 19mls of diluted wash buffer

3.Place on a roller for 10 minutes until dissolved

The sample is now ready to be applied to the Biochip

Simple Assay Protocol

Results are processed automatically using dedicated software

Competitive

Immunoassay

Format

• Conjugate – Known Analyte labelled with Horse Radish Peroxidase (HRP) Enzyme

• HRP – Reacts with Luminol and Peroxide to give off a light reaction

• Light signal inversely proportional to antigen concentration

Endpoint

measurement

• 2 chemicals – Luminol and Peroxide generate light reaction

• Light is generated by Chemiluminescence

• Charge Coupled Device Camera captures images

• Investigator quantifies images using Relative Light Units (RLU) given off by each DTR

• Correction and Reference Spots used for image validation

Validated Kits for Honey

• Antimicrobial Array I

• Antimicrobial Array II

• Antimicrobial Array III

• Antimicrobial Array III CAP Only

• Antimicrobial Array IV

Antimicrobial Array I

Antimicrobial Array II

Antimicrobial Array III

Antimicrobial Array III CAP only

Antimicrobial Array IV

New Array in

Development(May 2014)

Nitroimidazoles Array

• Dimetridazole

• Ronidazole

• 2-Hydroxymethyl-1-methyl-5-nitro-1H-imidazole (HMMNI)

• Metronidazole

• Metronidazole-OH  (MNZOH) 

• Ipronidazole

• Ipronidazole-OH (IPZOH) 

• Tinidazole   

• Propenidazole

• Ternidazole 

• Carnidazole

Advantages of

Investigator• More extensive testing menu

• Each Biochip kit is suitable for multiple sample types

• Higher throughput

• Biochip Reagents ready to use and safe to dispose

• Reduced matrix effects

Advantages of

Investigator •Less operator intervention (less probability of error / false positives)

•One incubation temperature for all Antimicrobial arrays

•Investigator false positive rates are less than 5%

•Quantitative result for each individual analyte

•Improved sensitivity

Summary • Semi-automated bench-top system

• Based on ELISA technology (multiplexed) and allows simultaneous multi-analyte assessment from a single honey sample

• Supplied as the complete package

• Simple sample preparation and easy to use, with consolidated testing over four arrays

• Unrivalled dedication to validating arrays for a number of different floral types

• Dedicated focus and support to all customers

• Cheaper pricing in comparison to sending all samples for confirmation - Intertek/ QSI

Your complete screening solution