1

Separations

Separation in based upon differential

migration between the stationary and

mobile phases.

Stationary Phase - the phase which

remains fixed in the column, e.g. C18,

Silica

Mobile Phase - carries the sample

through the stationary phase as it

moves through the column.

Injector

Detector

Column

Solvents

Mixer

Pumps

High Performance Liquid Chromatograph

Waste

2

Separations

Injector

Detector

Column

Solvents

Mixer

Pumps

Chromatogram

Start Injection

mAU

time

High Performance Liquid Chromatograph

3

Separations

Injector

Detector

Column

Solvents

Mixer

Pumps

Chromatogram

Start Injection

mAU

time

4

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

5

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

6

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

7

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

8

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

9

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

10

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

11

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

12

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

13

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

14

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

15

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

16

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

17

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

18

HPLC Applications

Chemical

Environmental

Pharmaceuticals

Consumer Products

Clinical

polystyrenes

dyes

phthalates

tetracyclines

corticosteroids

antidepressants

barbiturates

amino acids

vitamins

homocysteine

Bioscience

proteins

peptides

nucleotides

lipids

antioxidants

sugars

polyaromatic hydrocarbons

Inorganic ions

herbicides