• Background

• 1st , 2nd and 3rd FAO-APHCA/OIE Regional Workshop on

Brucellosis Diagnosis and Control with an Emphasis on Brucella melitensis (in collaboration with DLD)

• The 4th FAO-APHCA/OIE/DLD Regional Workshop on

Brucellosis Diagnosis and Control in Asia-Pacific Region

-Proficiency Test and Ways Forward-

Chiang Mai, 19-21 March 2014

• Brucellosis OIE Twinning Laboratory ProgrammeFrance-Thailand

• The 1st and 2nd Asia-Pacific Bovine Brucellosis

Proficiency Test (2013, 2014)

• Outcome and ways forward

The Minutes of 65th Executive Committee Meeting and the 29th

Session of APHCA (25 September 2005)

Thailand - Raised the re-appearance of Brucella melitensis

and its threat

- Requested APHCA to organise a relevant workshop

“ ……. Supported by delegates…….”

i) Brucella melitensis in goats –

diagnosis, vaccination and

other trade related

technical protocols

by B. Garin-Bastuji, c/o OIE;

ii) Special presentation on “OIE pathway for BSE-free status declaration”

by Y. Oketani, OIE-Tokyo Office; and

iii) General discussion on disease issues in goats

Report of the 30th APHCA Session (Luang Prabang, Lao PDR)

Wednesday, 25 October 2006 09 00 – 10 00 Review of day 1 – country priorities for action(s)

10 15 – 12 30 APHCA-OIE Regional workshop on goats (chaired by C. Leowijuk)

Minutes of the 31st Session of APHCA

October 29th -November 1st 2007 , Chiang Mai, Thailand

Future workplan/activities: 2008 period

• Continued activities from 2007

• New Initiatives: 1/4 topics …. Proposed

“Diagnosis and control of Brucella melitensis and Bluetongue”

1st FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis

Chiang Mai and Lampang, 20-23 October 2008

• 17 member countries• Lectures and lab practiced on serological tests• National labs: keep same SOP according to OIE standard

1st FAO-APHCA/OIE Regional Workshop

• Member countries: requested

- Follow up workshop/training

- Continue capacity building of national veterinary services

2nd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis

Khon Kaen, 8-11 June 2009

• 16 member countries

• Lecture and lab practiced – SOP (based on OIE Manual)

• RBT antigens were provided

Requested:

- Quality assurance including standardisation

- Strengthening of Regional collaboration on diagnosis and control of brucellosis

Recommendation for OIE Twinning Programme

2nd FAO-APHCA/OIE Regional Workshop

3rd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis

Sukhothai and Phitsanulok, 21-25 November 2010

• 17 member countries

• Lecture and lab practiced serological tests and reagents control

• Demonstration: Brucella spp. isolation, identification and typing (BSL-3)

Observation and suggestion:

• The member countries

- utilized the knowledge and techniques

- improved/strengthened their laboratory diagnosis capacity

• Under the OIE Twinning Programme

- the participants requested for support on Brucellosis proficiency testing to member countries in the region

3rd FAO-APHCA/OIE Regional Workshop

OIE Reference Laboratory Twinning Programme:

Brucellosis, France-Thailand

• Birth of collaboration………

- 1st FAO-APHCA/OIE Regional Workshop

- 2nd FAO-APHCA/OIE Regional Workshop

• The Brucellosis OIE Twinning Program France-Thailand (ANSES & NIAH-DLD)

- OIE expert: Dr. Bruno Garin-Bastuji, ANSES

- Three-year project: 2010-2013

OIE Reference Laboratory Twinning Programme: Brucellosis

• Brucellosis twinning programme was completed on December 31, 2013…

but collaboration still goes on………..

OIE Reference Laboratory Twinning Programme: Brucellosis

Thailand-Japan Joint Conference on Animal Health 2012

Prince Mahidol Award Conference, 28-31 Jan 2013, Bangkok

OIE Reference Laboratory Twinning Programme: Brucellosis

• NIAH and RVRDCs – poster presentations: brucellosis laboratory surveillance and Twinning for 6 posters

(Thailand-Japan Joint Conference on Animal Health 2012)

OIE Reference Laboratory Twinning Programme: Brucellosis

Preparation: The 1st Asia-Pacific Bovine Brucellosis Proficiency Test 2013

• Under OIE Twinning programme & FAO-APHCA and supervision by Dr. Bruno Garin-Bastuji

• Panels of sera preparation: July 2013

• Distribution

• Report the results in 2014

The 4th FAO-APHCA/OIE/DLD Regional Workshop on Brucellosis Diagnosis and Control in Asia-Pacific Region

-Proficiency Test and Ways Forward-Chiang Mai, 19-21 March 2014

• Review and update on brucellosis

• Quality management in veterinary testing lab

• International shipment

• PT 2013 results

• Distributed PT 2014

Workshop proposed:

Share information for strengthening the regional networking

FAO-APHCA and OIE RRAP should continue providing collaborative support to strengthen Brucellosis diagnostic capacity building

OIE/FAO Ref Lab should continue to provide technical support

Possible ….. Refresh/update hands-on WS on Brucellosis Diagnosis

(upon request)

• Participants 34 labs (results 33 Labs)

• Asia-pacific countries 18 countries, results returned 17 labs

• Regional VRDCs-NIAH 8 labs

• Government enterprise 1 lab

• Universities 4 labs

• Private companies 2 labs

• ANSES

Results: The 1st Asia-Pacific Bovine Brucellosis Proficiency Test 2013

The 1st and 2nd Asia-Pacific Bovine Brucellosis Proficiency Test (2013, 2014)

Outcome and ways forward…………

Animal Health Laboratories

- NIAH, Bangkok

- OIE Regional Reference Laboratory for FMD

- Veterinary Biological Assay Center /ASEAN Assay Lab

- 8 Regional Veterinary Research and Development Centers (7+1) Songkhla

Laboratory Quality assurance: NIAH & RVRDCs

Laboratory Quality assurance: NIAH & RVRDCs

- Serological test for Brucellosis was accredited: RBT and CFT (NIAH and 7 RVRDCs)

ISO 9001:2008

Brucellosis Research collaboration

• QIA-Korea and NIAH-DLD-Thailand: 2014-2016

“The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canisin Thailand”

• NIAH-Japan and NIAH-DLD-Thailand: June 2014-March 2017“Development of Milk-ELISA kit for detection of antibody against Brucella sp.”

@ Training @ Annual Meeting of Brucellosis Laboratories@ Further study: 1 MSc and 1 PhD (Brucellosis Lab)

PVS Evaluation, 2012 PVS Gap Analysis, 2014

20052007

• Discussion and needed for brucellosis diagnosis and control

20082010

• FAO-APHCA/OIE Regional Workshops (1st, 2nd and 3rd WS)

20102013

• OIE Lab Twinning Programmeon Brucellosis (France-Thailand)

20132014

• 4th FAO-APHCA/OIE/DLD Regional Workshop

• Bovine PT (2013 and 2014)

2008Present

•NIAH-DLD brucellosis related activities and lesson learned, ISO/IEC 17025:2005 and ISO 9001:2008, research collaboration

•FETPV, One Health

•OIE-PVS

20142015

• Application as OIE Ref Lab for Brucellosis

UnderstandingCommunication,

Team and trust

Coordination & Collaboration

Control brucellosis in the region

Brucellosis laboratory network in Asia-Pacific region

Regional Network and need support

from international organisations

Disease Control

• Regional Veterinary Research and Development Centers (8 Labs)

• Bureau of Disease Control and Veterinary Services, DLD.

• Bureau of Epidemiology, Department of Disease Control, MOPH

• National Institute of Animal Health

monayae@dld.go.thmonayae@gmail.com

bruno.garin-bastuji@anses.fr