SUPPLEMENTARY FIGURE LEGENDS

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Supplementary Figure 1. (a) NECs and CECs isolated from all five patients were incubated on Matrigel for 16 h after irradiation with 4 Gy, and

then photographed. (b) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, RT-PCR was used to

assess the mRNA expression levels of MMP2, plasminogen and the 18S rRNA. The experiments were repeated in triplicate and the data shown

represent typical results. (c) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, zymography was

used to analyze the activities of MMP-2 and MMP-9. The experiments were repeated in triplicate, and the data shown represent typical results. (d)

NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was used to analyze the generation of

angiostatin. The results from three independent experiments are expressed as means ± SEM.

Supplementary Figure 2. (a) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was

used to analyze the generation of IL-6. The results from three independent experiments are expressed as means ± SEM.

Supplementary Figure 3. NECs and CECs isolated from patient A were incubated on Matrigel for 16 h after irradiation with various doses of IR

(2, 3, 4, 6, 8 or 10 Gy), and then photographed.

Cont 4 Gy

Patient A

Cont 4 Gy

Patient B

Cont 4 Gy

Patient C

Patient D Patient E

Cont 4 Gy Cont 4 Gy

Supplementary figure 1.

A B C D E

18S rRNA

Patient

- + - + - + - + - +

18S rRNA

Plasminogen

A B C D EPatient

4 Gy - + - + - + - + - +

Zymography

4 Gy - + - + - + - + - +

A B C D EPatient

(C) (D)

A B C D EPatient A B C D E

NECs CECs

Generation of angiostatin

Generation of IL-6

A B C D EPatient A B C D E

NECs CECs

Control 2 Gy 3 Gy 4 Gy 6 Gy 10 Gy

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