Work done a Centre for Soft and

Condensed Matter.

Fabrication of glass nano-pipettes and micro-pipettes

Pore diameter: 104.7 nm Shrunk diameter: 74.36 nm

Pore diameter: 88.62 nm Shrunk diameter: 54.78 nm

Shrinking of glass nanopores

Device characterization

I-V curves for 5 salt buffer concentrations. Using the I-V curves calculate the

conductance.

Experimental Set up

and reading

Pore diameter = 176.1 nm

Molarity Resistance (MΩ) Irms (Noise) pA Beta value (β) Alpha Value (α)

1M 2.13 8.47 0.1 0.5

0.1M 7.75 3.94 0.1 0.5

50mM 11.34 4.33 0.1 0.5

1mM 87.53 3.91 0.1 0.5

100µM 95.99 3.41 0.1 0.5

1µM 98.15 3.87 0.1 0.5

Results

Flow cell designed for imaging pipettes

under 20X and 40X objective

Micro fluidic cell designed for pressure and

diffusion experiments through pipettes.

Flow cell designed for visualizing electrical and pressure

driven translocation events through pipettes.

Oscillation of 1 micron polystyrene beads in

alternating electric field.

Position A Position B

Polystyrene

bead

Function

generator

Calculating the velocity of 1 micron polystyrene

beads oscillating in alternating electric field.

1a 1b 1c 1d

5a 5b 5c 5d 5e

Translocation of Polystyrene beads through

sub micron glass pipettes

Fig (1a-1d) translocation of 15 micron beads through 50 micron pipette

Fig (5a-5e) translocation of fluorescent 15 micron beads through 30 micron pipette

2a 2b 2c 2d

3a 3b 3c 3d 3e

4a 4b 4c 4d

Fig (2a-2d); (3a-3e); (4a-4d) – Translocation of 3 micron beads through 5 micron pipette

Pressure driven Translocation of 450 nm polystyrene beads through a 900 nm pipette,

image taken under 40X objective.

Pressure driven diffusion through pipettes to track the

trajectory of polystyrene beads at the mouth of the

micropipette

Pore diameter: 1.6 µm

Translocation and visualization of polystyrene beads

(500 nm and 1 µm) under alternating electrical stimulus.

Flow cell used for visualizing and performing translocation events.

1 2 3

4 5 6

7 8

Visualizing a single electrical translocation event, of a 1 micron polystyrene bead oscillating

under alternating field at 3V.

1

5

2

4 3

6 7