Yeast Metabolismfor

Brewers and Beer Geeks

1Tuesday, April 24, 2012

"There are no applied sciences.There are only applications of science

and this is a very different thing.The applications of science is easy to

one who is master of the theory of it."

Louis Pasteur

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Glycolysis

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ADH and PDC - The Best Enzymes EVAR!

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Other Fates of Pyruvate

ATP

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Weird Yeast Metabolism 'Effects'

• The Pasteur Effect• The Crabtree Effect• Brewing Yeast are essentially always

using fermentative metabolism

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The Citric Acid Cycle

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Glycerol Production

NADH

NAD+

Glycerol

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Wort Carbohydrate Composition

Glucose

Fructose

Sucrose

Maltose

Maltotriose

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Brewing Yeast Carbohydrate Selectivity

From Brewing Yeast and Fermentation p.74

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What about nitrogen?

• Wort nitrogen compounds (Ingledew, 1975):o protein 20%o polypeptides 30-40%o amino acids 30-40%o nucleotides 10%

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Transaminase Reactions

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Fusel Alcohols

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Esters

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Vicinal diketones

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Vicinal diketones

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Allain Lab Research

Natural vs. Artificial Carbonation of BeerIs there a difference?If so ... what is the difference?

Effect of yeast pitch rate on production of flavor and aroma compounds.

Online Beer Analysis Database And Statistics Server (BADASS)

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Sulfur compounds

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Sulfur compounds

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