Post on 28-Dec-2015
transcript
Yong HuangDepartment of Mechanical
Engineering
Clemson University, Clemson, SC
Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Background Inkjet-based
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[Boland, 2007]
Inkjetting of CaCl2 into 2% alginic acid to make a branched structure
[Nakamura, 2008]
Inkjetting 0.8% sodium alginate into
2% CaCl2 to make a tube with the help of gravity
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[Skardal, 2010]
Printing of agarose and 3T3 cell-containing PEGTA filaments to make a tube
[Norotte, 2009]
Printing of agarose and smooth muscle cell (SMC) cylinders to make a tube
Background Filament-based
Background Laser-based
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Laser printing of alternating layers of fibroblasts (green) and keratinocytes (red) to form a grid structure (Scale bar = 500 µm)
[Koch, 2012]
Challenges
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How to scale-up scaffold-free bioprint complex structures such as overhang structures?
How to evaluate and mitigate the post-printing cell damage?
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Objective
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Needs in cellular tube bioprintingVascularization as a main technological barrier for building
3D organs
Tube printing - a logical initial step towards vascularization
Objective
To scaffold-free fabricate viable cellular tubes using inkjet and laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Liquid levelFirst layerSecond layer
3T3 cell suspension
Tube Inkjetting Experimental setup
Tube Inkjetting - Zig-zag tube
Fabrication process: bottom part, overhang part and top part
Wall thickness: 150~200 µm
Tube diameter: 3 mm and length: 10 mm
Zigzag tube fabrication
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Tube Inkjetting - Cell viability test
(a) Printed tube, (b) tube surface and (c) cell viability test after liquefying
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(a)
Cells
(b)
Live Cell
Dead Cell
After liquefying
(c)
Cell viability immediately after printing: > 85% Cell viability after 3 days: > 80% Cell viability comparable to that of the control
Tube Inkjetting - Cell viability
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0 24 48 7270
75
80
85
90
95
Cel
l via
bilit
y %
Post-printingControl
(Hours)
Tube Inkjetting - Other structures
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Pyramid Cone
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Tube laser printing - Experimental setup
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ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
Tube laser printing - Experimental setup
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Direct-writing height = 1 mm
ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
Tube laser printing - results
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Top viewSide view
Φ=3 mm
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Overhang structures can be scaffold-free fabricated
Viable cellular tubes (3T3) can be printed
ConclusionsInkjetting
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Tubes can be scaffold-free fabricated using LIFT-based laser printing technology
Highly viscous materials (alginate) can be laser printed into well-defined tubular structures
Laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Laser printing of 3D cellular tubes
Printing of adipose-derived stem cell tubes
Future WorkFabrication
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Apoptosis/necrosis test, cell proliferation capacity, and phenotype variation
Post-printing fusion of tubular tissue
Mechanical property measurement of fabricated tubes
Post-printing evaluation
Thanks
and questions?
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