© 2011 Fluxion Biosciences. All rights reserved.

Brief timeline and flunding history

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Fluxion today

BioFlux 200 BioFlux 1000 IonFlux-16 IonFlux HT

• Company:– Venture backed since Dec 2007– 4 products commercialized and selling globally– Segment: Biotools– Over 100 units installed

• Products: instruments and consumables– BioFlux System for cell-cell interactions– IonFlux Automated Patch Clamp System– IsoFlux rare cell isolation platform – under development

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Partial customer list

Johnson & Johnson

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IonFlux applications

• Compound profiling– Dose response measurements– Hit confirmation– Single concentration screening

• Mutant screening – Evaluate functional changes dependent on mutations– Record responses from up to 32 distinct mutants (8 for IF-HT)

• Structure-function studies– Determine the effect of mutations on channel function– Characterize expression for differentiated stem cells– Recording endogenously expressed ion channels– Screen clones based on ion channel density

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IonFlux Technology for APC- Best in Class

• High quality data– G-seal quality recordings– Ensemble recording for improved

success rate

• Cost effective– Perform APC for about the cost of an

MPC setup

• Simple to use– Ease of use of a ‘plate reader’– Relevance of patch clamp data– Table top unit

• Reliable– No robotics required– Only two moving parts

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Ensemble vs. single cell recording

• Ensembles average signals over 20 patched cells (16 ensembles per plate, left panel)

• The new single cell consumable records from one cell per channel (16 cells per plate for IF-16, right panel)

• The single cell consumable utilizes the same hardware

20-cell ensemble Single cell

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Well-Plate Integrated Microfluidics

Well-plate integrated microfluidics (WPM) utilizes standard 96- and 384-well plates, but the bottom of the plate is replaced with a microfluidic network interconnected between the wells.

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* Sample customer slide – Pfizer UK

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High Level Timeline

Founded

2006 2007 2008 2009 2010 2011

Grant 1 Ph2

Grant 1 Ph1

Grant 2 Ph2

Grant 2 Ph1

BioFlux product launch

IonFlux product launch

Series A Series B

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Grant pipeline and success rates

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SBIR: the good, the bad and the ugly

• The good:– Non-dilutive capital!– Can be the catalyst for new projects, encourages innovation– Early $$, which are the hardest

• The bad:– Long timelines: 9 mo. from application to funding– <200k is low capital for Phase I awards– Can be a distraction from the central mission

• The ugly:– Review process dependent on random events– In our case, projects with the greatest commercial worth didn’t get funded