© 2015 BioLife Solutions, Inc.© 2015 BioLife Solutions, Inc.

HypoThermosol®

Performance Evidence

© 2015 BioLife Solutions, Inc.

5-Day Hypothermic Storage of Human DermalFibroblasts Enabled by HypoThermosol®

alamarBlue® stain for metabolic activity

* Contains serum

Assayed 24 Hours Post Storage - % Recovery of Viable Cells

DAY STORAGE1 DAY STORAGE3 DAY STORAGE5

© 2015 BioLife Solutions, Inc. 4

Representative Fluorescent Micrographs Illustrating Morphology of Normal Human Dermal Fibroblasts

Stored in HypoThermosol®

Green Actin CytoskeletonPhalloidin (FITC)

Red Mitochondria ActivationMitoTracker® Red

Blue Nuclear StainHoechst 40X magnification

Media with Serum

3 Day Storage

5 Day Storage

Celsior®

3 Day Storage

5 Day Storage

Viaspan®

3 Day Storage

5 Day Storage

HypoThermosol®

3 Day Storage

5 Day Storage

© 2015 BioLife Solutions, Inc. 6alamarBlue® stain for metabolic activity

* Contains serum

Extended Hypothermic Storage of Human MesenchymalStem Cells Enabled by HypoThermosol®

Assayed 24 Hours Post Storage - % Recovery of Viable Cells

DAY STORAGE1 DAY STORAGE3 DAY STORAGE5 DAY STORAGE7

© 2015 BioLife Solutions, Inc. 7

Representative Fluorescent Micrographs Illustrating Morphology of Human Mesenchymal Stem Cells Stored in HypoThermosol®

Green Actin CytoskeletonPhalloidin (FITC)

Red Mitochondria ActivationMitoTracker® Red

Blue Nuclear StainHoechst 20X magnification

Normosol®-R

3 Day Storage

5 Day Storage

Plasma®-Lyte

3 Day Storage

5 Day Storage

Viaspan®

3 Day Storage

5 Day Storage

HypoThermosol®

3 Day Storage

5 Day Storage

© 2015 BioLife Solutions, Inc. 9

Recovery of Human Osteoblast Cells Following Hypothermic Storage Assayed 24 Hours Post Storage

Differentiated Following 5-Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol® Assayed 24 Hours Post Storage - % Recovery of Viable Cells

DAY STORAGE1 DAY STORAGE3 DAY STORAGE6

alamarBlue® stain for metabolic activity

* Contains serum

© 2015 BioLife Solutions, Inc. 10

Representative Fluorescent Micrographs Illustrating Morphology of Human Osteoblast Cells Following Hypothermic Storage

20X magnification

Differentiated Following 5 Day Hypothermic Storage ofUndifferentiated hMSC in HypoThermosol

Green Actin CytoskeletonPhalloidin (FITC)

Red Mitochondria ActivationMitoTracker® Red

Blue Nuclear StainHoechst

Normosol®-R

3 Day Storage

6 Day Storage

Plasma®-Lyte

3 Day Storage

6 Day Storage

Viaspan®

3 Day Storage

6 Day Storage

HypoThermosol®

3 Day Storage

6 Day Storage

© 2015 BioLife Solutions, Inc. 12

24 Hour Post Storage Recovery of Human Dental PulpStem Cells Following Hypothermic Storage

Commercial Solution Comparison: Serum and Serum-Free Media

DAY STORAGE1 DAY STORAGE3 DAY STORAGE5

% Recovery of Viable Cells

alamarBlue® stain for metabolic activity

* Contains serum