0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

100 nM Paclitaxel

0

2

4

6

8

Control

Paclita

xel 1

00 nM

RFU

(Arb

itrar

y U

nits

)

Caspase-9 activity Caspase-8 activity

MCF-7

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Z-VAD-FMK + Paclita

xel

Z-IETD-FMK + Pac

litaxe

l

Z-LEHD-FMK + Pac

litaxe

l

% A

nnex

in V

-pos

itive

cel

ls

Figure S1a

MCF-7

Figure S1b

0102030405060708090

100

Control

Paclita

xel

Bim si

RNA

Paclita

xel +

Bim

siRNA

Paclita

xel +

ABT-73

7+ B

im siR

NA

Bmf siR

NA

Paclita

xel +

Bmf s

iRNA

Paclita

xel +

ABT-73

7+ B

mf siR

NA

ABT-737

ABT-737 +

Bim

siRNA

ABT-737 +

Bmf s

iRNA

Paclita

xel +

ABT-73

7

% A

nnex

in V

-pos

itive

cel

ls

***

- - + + - -

- + - + - +Paclitaxel 100 nM

Bad siRNA

Scrambled siRNA - - - - + +

Bad

Actin

IP: Bcl-2

IB: Bcl-2

Input

IB: Bcl-2

Bcl-2

Bcl-2

Actin

IP: Bcl-2

IB: Bim

BimELBimLBimS

MCF-7

Figure S1c

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

100 nM Paclitaxel

Cleaved Caspase-3

0

2

4

6

8

RFU

(Arb

itrar

y U

nits

)

Caspase-9 activity Caspase-8 activity Caspase-3 activity

T47D

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Z-VAD-FMK + Paclita

xel

Z-IETD-FMK + Pac

litaxe

l

Z-LEHD-FMK + Pac

litaxe

l

% A

nnex

in V

-pos

itive

cel

ls

Figure S1d

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

100 nM Paclitaxel

Cleaved Caspase-3

0

2

4

6

8

RFU

(Arb

itrar

y U

nits

)

Caspase-9 activity Caspase-8 activity Caspase-3 activity

MDA-MB-468

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Z-VAD-FMK + Paclita

xel

Z-IETD-FMK + Pac

litaxe

l

Z-LEHD-FMK + Pac

litaxe

l

% A

nnex

in V

-pos

itive

cel

ls

Figure S1e

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

100 nM Paclitaxel

Cleaved Caspase-3

0

2

4

6

8

RFU

(Arb

itrar

y U

nits

)

Caspase-9 activity Caspase-8 activity Caspase-3 activity

BT20

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Z-VAD-FMK + Paclita

xel

Z-IETD-FMK + Pac

litaxe

l

Z-LEHD-FMK +

Paclita

xel

% A

nnex

in V

-pos

itive

cel

ls

Figure S1f

IP: Bcl-xL

IB: Bcl-xLBcl-xL

Bim ELBim LBim S

IP: Bcl-xL

IB: Bim

Mcl-1

Paclitaxel 100 nM

0 4 8 12 [h]

Bim ELBim LBim S

IP: Mcl-1

IB: Bim

Paclitaxel 100 nM

0 4 8 12 [h]

IP: Mcl-1

IB: Mcl-1

Figure S2a

MCF-7

Figure S2b

Pre-

IP

Post

-IP

BimELBimLBimS

HM LM S-100

Untreated Taxol 12 h

CoxIV

BiP/Grp78

BimELBimL

BimS

1 0.43

Actin

Ratio

Paclitaxel 100 nM

- +

Pre-

IP

Post

-IP

Actin

BimELBimLBimS

Untreated

1 1.39Ratio

HM LM S-100

MCF-7 MCF-7MCF-7

- + - +

Paclitaxel 100 nM

MCF-7 MCF-7 Bcl-2+

FLAG-Bcl-2

Bcl-2FLAG-Bcl-2

BimEL

BimL BimS

IP: FLAG

IB: Bcl-2

Input

IB: Bcl-2

IP: FLAG

IB: Bim

Bmf

Bmf

IP: FLAG

IB: Bmf

Input

IB: Bmf

BimELBimL

BimSInput

IB: Bim

Actin

IP: FLAG

IB: Bax

IP: FLAG

IB: Puma

*

IP: FLAG

IB: Bak

IP: FLAG

IB: Bad

- + - +

Paclitaxel 100 nM

MCF-7 MCF-7 Bcl-2+

Bad

Puma

Bax

Bak

Figure S2c

Figure S2d

IP: Bcl-2

IB: BaxBax

IP: Bcl-2

IB: Bak

Paclitaxel 100 nM

- +

IP: Bcl-xL

IB: Bax

IP: Bcl-xL

IB: Bak

Paclitaxel 100 nM

- +

IP: Mcl-1

IB: Bax

IP: Mcl-1

IB: Bak

Paclitaxel 100 nM

- +

Bak

Bax

Bak

Bax

Bak

MCF-7

0 4 8 12 24 48 0 4 8 12 24 48 (hr)

MitoCyto

Cytochrome c

CoxIV

T47D

Figure S3a

0 4 8 12 24 48 0 4 8 12 24 48 (hr)

MitoCyto

MDA-MB-468

Figure S3b

Cytochrome c

CoxIV

0 4 8 12 24 48 0 4 8 12 24 48 (hr)

MitoCyto

BT20

Figure S3c

Cytochrome c

CoxIV

0 4 8 12 24 48 (hr)

Bax

Bak

IP: Bax 6a7

IB: Bax N20

IP: Bak Ab-2

IB: Bak G23

100 nM Paclitaxel

T47D

Input

IB: Bax N20

Input

IB: Bak G23

Bax

Bak

Actin

Figure S4a

0 4 8 12 24 48 (hr)

Bax

Bak

IP: Bax 6a7

IB: Bax N20

IP: Bak Ab-2

IB: Bak G23

100 nM Paclitaxel

MDA-MB-468

Input

IB: Bax N20

Input

IB: Bak G23

Bax

Bak

Actin

Figure S4b

0 4 8 12 24 48 (hr)

Bax

Bak

IP: Bax 6a7

IB: Bax N20

IP: Bak Ab-2

IB: Bak G23

100 nM Paclitaxel

BT20

Input

IB: Bax N20

Input

IB: Bak G23

Bax

Bak

Actin

Figure S4c

Puma

Actin

- - + + - -

- + - + - +Paclitaxel 100 nM

Puma siRNA

Scrambled siRNA - - - - + +

MDA-MB-468

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Puma siR

NA

Paclita

xel +

Pum

a siR

NA

Scram

bled s

iRNA

Paclita

xel +

Scra

mbled si

RNA

% A

nnex

in V

-pos

itive

cel

ls

Figure S5a

Puma

Actin

- - + + - -

- + - + - +Paclitaxel 100 nM

Puma siRNA

Scrambled siRNA - - - - + +

BT20

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Puma siR

NA

Paclita

xel +

Pum

a siR

NA

Scram

bled s

iRNA

Paclita

xel +

Scra

mbled si

RNA

% A

nnex

in V

-pos

itive

cel

ls

Figure S5b

Puma

Actin

- - + + - -

- + - + - +Paclitaxel 100 nM

Puma siRNA

Scrambled siRNA - - - - + +

MCF-7

0

10

20

30

40

50

60

70

80

90

100

Control

Paclita

xel

Puma siR

NA

Paclita

xel +

Pum

a siR

NA

Scram

bled s

iRNA

Paclita

xel +

Scra

mbled si

RNA

% A

nnex

in V

-pos

itive

cel

ls

Figure S5c