MICE MRIMICE MRI

New Data

AIMSAIMS

This study is for imaging aortic atherosclerotic plaques in Apo E deficient mice

In addition to study of Apo E atherosclerotic mice this effort is meant to provide necessary technical experiences for rabbit study soon to be approved and transferred to Galveston

MRI MRI withwith

Contrast (Magnevist)Contrast (Magnevist)In order to be sure that we are looking to the

aorta,we decided to inject contrast media

We had to inject Magnevist to mouse while inside the magnet, so we tried to cannulate femoral vein, jugular vein, intracardiac approach

In order to decrease the motion effect of respiration,we had to use respiratory gated MRI

To have respiratory gating,we needed to Intubate the mouse and use ventilation

The MR Image characteristicsThe MR Image characteristics

Coil:5 and 9cmField of view:6 cmMatrix(nv):256Spin Echo:Tr=2500,Te=12, Slice=17-24Movi(while injecting the Gadelenium) :

Tr=200, Te= 5

3/103/10

MRI Parameters for MRI Parameters for Movi Images Movi Images

After injection of Magnivest Tr=200, Te=5

Soft ware analysis to show the Soft ware analysis to show the flow and find the aortaflow and find the aorta

Spin Echo Images:Spin Echo Images:Tr=2500, Te=12Tr=2500, Te=12

nv=128nv=128

High resolution spin echo High resolution spin echo imagesimages

Slice thickness :1.6 ,Gap:0.5Slice thickness :1.6 ,Gap:0.5Tr=2500, Te=12, nv=256Tr=2500, Te=12, nv=256

03/1303/13

3/14mvcor3/14mvcor

Sems tranSems tran

SPIO InjectionSPIO Injection

With injection of Feridex with maximum dose(3mol/kg),we had some problems: because of small blood content of mouse, dilution wasn’t enough

It caused too much decrease in T2 , so although we had approximately good resolution, we think that by decreasing the dose we can find better images

Future PlansFuture Plans

Injection of 40mol/kg of Feridex

ECG gated MRI (we have the motion effect of heart in chest region)

Starting rabbit study