07h7901_CELL-DYN 1800

INTERFACE SPECIFICATION

CELL-DYN 1800 SYSTEM

LIST NO. 07H79-01 REVISION A

ABBOTT DIAGNOSTICS DIVISION Abbott Laboratories

Abbott Park, IL 60064, USA

INTERFACE SPECIFICATION

CELL-DYN 1800 SYSTEM

Abbott Laboratories 2003 Abbott Diagnostics Division Abbott Park, IL 60064, USA 07H79-01AFebruary 2003

CELL-DYN 1800 System Interface Specification 07H79-01February 2003 Page i

TABLE OF CONTENTS

1.0 Introduction..................................................................................................................... 1

2.0 Mechanical Interface ...................................................................................................... 1

3.0 Electrical Interface .......................................................................................................... 2

4.0 Data Interface................................................................................................................. 2

5.0 Communication Protocol ................................................................................................ 3

6.0 Identification (ID) Segment ............................................................................................. 5

7.0 Results Segment ............................................................................................................ 8

Interface Specification CELL-DYN 1800 System Page ii 07H79-01February 2003

NOTES

CELL-DYN 1800 System Interface Specification 07H79-01February 2003 Page 1 of 36

1.0 Introduction

1.1 Purpose

This document describes the interfacing characteristics of the Abbott CELL-DYN 1800 automated hematology analyzer when attached to a Host.

1.2 Definitions and Conventions

Host: external computer or data collection system.

CELL-DYN 1800: Abbott CELL-DYN 1800.

This specification follows guidelines adopted at the Biomedical Instrumentation Interface Standards Conference held at the University of Florida in December 1980 and at the University of Texas in Dallas, April 1981.

Signal designators and related characteristics follow EIA Standard RS232C as summarized in The Handbook of Computers and Computing, Seidman and Flores, Van Nostrad Reinhold, 1984; and Integrated Circuits Applications Handbook, Arthur H. Seidman, John Wiley & Sons, 1983.

Numeric equivalents of characters are shown as hexadecimal (hex) values.

2.0 Mechanical Interface

2.1 Connector arrangement

The CELL-DYN 1800 provides a standard DB-9 male connector, labeled COM1, mounted on the side of the instrument.

Pins on the DB-9 connector: (standard RS-232 9 pin assignments)

Pin 1: Data Carrier Detect (DCD input) (monitored) Pin 2: Data to CELL-DYN 1800 (RD-receive data) (ACK/NAK, XON/XOFF) Pin 3: Data to Host (LIS, results) (TDtransmit data) Pin 4: Data Terminal Ready (DTR) (set true) Pin 5: Signal Ground Pin 6: Data Set Ready (DSR) (monitored) Pin 7: Request to Send (RTS output) (set true) Pin 8: Clear to Send (CTS input) (monitored, if requested in Setup) Pin 9: Ring Indicate (RI input) (unused)

Interface Specification CELL-DYN 1800 System Page 2 of 36 07H79-01February 2003

3.0 Electrical Interface

3.1 Voltage levels and electrical characteristics are as defined by the EIA RS-232C specification.

3.2 The maximum recommended cable length is 30 meters, or 100 feet. The actual maximum workable cable length is dependent on the environment of the site, the selected baud rate, and the equipment being connected together.

4.0 Data Interface

The system can be configured for data transmission using the COMPUTER SETUP Menu. The configurable transmission parameters include data bits, stop bits, parity and baud rate.

4.1 The asynchronous method of data transmission (serial by bit) is used.

4.2 All information transmitted is in character form and is represented by 7-bit or ASCII.

4.3 Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (least significant first), one or no parity bit, and one (1) or two (2) stop bits.

4.4 Parity may be selected as none, odd, or even.

4.5 The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per second (bps).

4.6 With the exception of the control characters mentioned in Section 5.2, only printable ASCII characters (hex 20 to hex 7E) are used in a message.


5.0 Communication Protocol

5.1 Communication Modes

5.1.1 RUN Menu Automatic Transmit Mode

The User may select the Automatic Transmit mode on the COMPUTER SETUP Menu. This mode allows the automatic transmission of results during the RUN cycle. If Automatic Transmit mode has been selected, the user may also choose whether or not to transmit histograms along with the count data.

5.1.2 DATALOG Menu Transmit Mode

On the DATALOG Menu, the user may select results from a single sample or from multiple samples for transmission. Only count data may be transmitted from the Data Log menu.

5.1.3 DISPLAY SPECIMEN Menu Transmit Mode

On the DISPLAY SPECIMEN Menu, the user may request transmission of results from the specimen being displayed. Count data are transmitted. Histograms are also transmitted if the Automatic Transmit mode is selected in the COMPUTER SETUP Menu.

5.2 Transmission Control

Transmission control is provided in two ways: 1) XOFF/XON protocol in which the Host transmits an XOFF character (hex 13) to stop transmission from the System and an XON character (hex 11) to re-start transmission; and 2) CTS (Clear To Send) hardware control.

The XOFF/XON protocol has a 1.5 second time-out. If no XON is received for 1.5 seconds after an XOFF, then transmission resumes anyway.

The CTS hardware control has no time-out. That is, no data transmission will be started with CTS false. However, the transmission will still time-out if at any one time CTS remains false for longer than the time-out selected in the COMPUTER SETUP Menu (see Section 5.3.1 below). This is a different use and meaning for time-out, but it is needed to prevent the Host from indefinitely delaying the CELL-DYN 1800 from processing the next sample. Re-transmission requests are also supported and discussed in Section 5.3.


5.3 Response from Host

5.3.1 Between-Transmission Time-out

The time-out interval after transmission of one message is programmable in the COMPUTER SETUP Menu from 100 milliseconds to 9.9 seconds in 100 millisecond increments.

NOTE: The timer starts just as the ETX, which ends the message, is transmitted.

5.3.2 Release for Next Message

The Host can release the CELL-DYN 1800 to send the next message by sending an ACK (06 hex) before the time-out interval passes. Otherwise, the CELL-DYN 1800 will begin transmission of the next message at the end of the time-out interval in 5.3.1 above.

5.3.3 Re-transmission

If for any reason the Host requires re-transmission of the message, it signals the CELL-DYN 1800 by sending a NAK (15 hex) before expiration of the time-out. A message will be sent by the CELL-DYN 1800 a maximum of three (3) times. After that, the same conditions prevail as after a time-out. The Host may (it is desirable but not mandatory) acknowledge the re-transmission with ACK or NAK. If ACK is sent, the CELL-DYN 1800 will immediately start processing the next record (if any). If NAK is sent, the record will NOT be transmitted a third time. Receipt of either the ACK or NAK will allow the CELL-DYN 1800 to move to the next record more quickly.

5.4 Message Format

The message format consists of the following elements:

STX, ID SEGMENT, RESULT SEGMENT, CHECK SUM, ETX.

5.4.1 There are four (4) types of messages, each distinguished by its ID segment:

WBC Histogram Message

RBC Histogram Message

PLT Histogram Message

Count Data Message

5.4.2 The four messages taken together represent all the results of testing a single specimen.

5.4.3 Each message begins with STX (hex 02).

5.4.4 The Identification (ID) segment is described fully in Section 6.

5.4.5 The Result segment is described fully in Section 7.

5.4.6 The Check Sum is always provided and may be optionally processed by the Host to verify correct transmission. It is generated by taking the modulo-256 sum of all the characters in the message except the STX and ETX characters. The two-digit hexadecimal representation of the Check Sum byte is placed immediately before the ETX as two ASCII characters.

5.4.7 The ETX character (hex 03) is the last character of the message.


5.4.8 Message Length

Each of the three Histogram messages is 1204 characters long. The Count Data message is 406 characters long. These message lengths count all characters from STX to ETX inclusive.

5.4.9 Data Representation

Numeric data are transmitted in fields of fixed length with zeros used to fill empty spaces on the left. Out-of-range numeric values are represented by strings of ">" characters (hex 3E), and undefined numeric values are represented by strings of "" characters (hex 2D). Alphanumeric data are transmitted in fields of fixed length enclosed in double quotation marks (hex 22). Within the quotation marks, the data are right-justified and blanks (hex 20) are used to fill empty spaces. Fields are separated by commas (hex 2C).

6.0 Identification (ID) Segment

6.0.1 For counting purposes, the STX mentioned in Section 5.4.3 is taken as byte 1.

6.1 The Identification segment of each message identifies the type of message and the specimen the message represents.

6.2 Message TypeField 1

The Message Type field identifies the message. There are four message types:

WBC Histogram Message Type "WBC"

RBC Histogram Message Type "RBC"

PLT Histogram Message Type "PLT"

Count Data Message Type "__ _" (3 blanks)

6.3 Instrument TypeField 2

The Instrument Type is an alphanumeric field of seven (7) characters enclosed in double quotation marks. The string for the CELL-DYN 1800 is sent as " CD1800".

6.4 Serial NumberField 3

The Instrument Serial Number is an alphanumeric field of twelve (12) characters enclosed in double quotation marks. It is not available in this release, so the field is transmitted as " " (twelve dashes).

6.5 Sequence NumberField 4

The Sequence Number is a numeric field of four (4) characters with a value ranging from 0 to 9999.


6.6 Specimen TypeField 5

The Specimen Type is the fifth field in the Identification Segment. It occupies a numeric field of two characters whose value identifies the specimen type as follows:

00: Patient type "PATIENT" 01: Replicate 1 "REPLIC 1" 02: Replicate 2 "REPLIC 2" 03: Replicate 3 "REPLIC 3" 04: Replicate 4 "REPLIC 4" 05: Replicate 5 "REPLIC 5" 06: Replicate 6 "REPLIC 6" 07: Replicate 7 "REPLIC 7" 08: Replicate 8 "REPLIC 8" 09: Replicate 9 "REPLIC 9" 10: Low Control "LOW CTRL" 11: Normal Control "NOR CTRL" 12: High Control "HI CTRL" 13: Background "BACKGRD" 14: Electrical Background "ELEC BKGD" 15: Calibrator "CALIBRATR" 16: Gain Adjustment "GAIN ADJ" 17: Auto Calibration "AUTO CAL"

6.7 Operator IDField 6

The Operator ID is a alphanumeric field of three (3) digits enclosed in double quotation marks. If no Operator ID is specified, the field is transmitted as " ".

6.8 Specimen DateField 7

The Specimen Date, giving the date on which the specimen was run, is an alphanumeric field of eight (8) characters enclosed in double quotation marks. The default format of the date is MM/DD/YY, where MM represents the month in two digits, DD represents the day of the month, and YY represents the year. The date format can be changed in the DATE/TIME Menu.

6.9 Specimen TimeField 8

The Specimen Time is a alphanumeric field of five (5) characters enclosed in double quotation marks. It gives the time at which the specimen was run in standard 24-hour format.

6.10 Specimen IDField 9

The Specimen ID is an alphanumeric field of sixteen (16) characters enclosed in double quotation marks. If the specimen is a patient specimen, the Specimen ID is entered by the operator. Otherwise, the Specimen ID is generated by the CELL-DYN 1800 to identify special types of samples, such as controls. If not specified, the Specimen ID is transmitted as " " (16 dashes). See Section 6.6 (Field 5).


6.11 Specimen NameField 10

The Specimen Name is the tenth field in the Identification Segment. It occupies a text field of sixteen (16) characters enclosed in double quotation marks, and it is undefined except for patient samples.

6.12 Specimen SexField 11

The Specimen Sex field consists of one (1) character M or F enclosed in double quotation marks. If not entered by the operator, Specimen Sex is transmitted as a blank " ".

6.13 Specimen Date of Birth (DOB)Field 12

The Specimen Date of Birth field has an identical format to Field 7, except that Field 7 is automatically generated by the CELL-DYN 1800. If DOB is not entered by the operator, it is transmitted as "bb/bb/bb" where the bbs are actually 2 ASCII blanks each.

6.14 Dr NameField 13

The Dr Name field is an alphanumeric field of twenty two (22) characters enclosed in double quotation marks.

6.15 Collection DateField 14

The Collection Date field is an alphanumeric field of five (5) characters enclosed in double quotation marks. The format is MM/DD, where MM represents the month in two digits and DD represents the day. The year is not included. It gives the date at which the specimen was collected. If the Collection Date is not entered, it is transmitted as "bb/bb" where the bbs are 2 ASCII blanks as in Field 12 above.

6.16 Collection TimeField 15

The Collection Time field is an alphanumeric field of five (5) characters enclosed in double quotation marks. It gives the time at which the specimen was collected. If the Collection Time is not entered, it is transmitted as "bb:bb" (bbs are 2 ASCII blanks each and : is the middle byte).

6.17 CommentField 16

The Comment field is an alphanumeric field of sixteen (16) characters enclosed in double quotation marks.

NOTE: Fields 1 to 16 above are common in both format AND actual content for a given sample and, as such, are transmitted multiple times when histogram transmission is requested (firstif sentwith all three histograms and then again with the count record). Given todays typical transmission speeds and the lengths of the rest of the fields, the convenience of having the header information combined with each data group has been chosen over the absolute smallest or fastest transmission. Also, in the time frame of even normal sample processing, the transmissions (at 9600 baud) should take less than 4 seconds out of the 60 second (nominal) cycle time.


7.0 Results Segment

7.1 The results for each specimen are sent in the format described. All numeric fields are integers, and some need to be scaled by the Host.

7.2 Histogram Messages

Refer to Table 1, Histogram Messages, and Table 2, Histogram Record Dump.

7.2.1 Scale FactorField 17

The Scale Factor is a numeric field of five (5) characters. It is not implemented at this time and is therefore always transmitted as 00000 (5 ASCII zeros without the surrounding double quotes).

7.2.2 Channel DataFields 18 through 273

The Channel Data fields are numeric fields of three (3) characters each, giving normalized counts for every channel of the designated histogram (WBC, RBC, or PLT). Data for each channel will occupy 4 bytes total since there will be a comma between each 3-digit value. Each value is right-justified with ASCII zero 0 (not null) characters to keep the fields a fixed length. So a channel value of binary zero is transmitted as three ASCII zero characters (000).

NOTE: A comma (,) follows the last field which immediately precedes the CRC characters.

7.3 Count Data Message

Refer to Table 3, Count Data Messages, and Table 4, Count Record Dump.

The parameters reported by the instrument may be represented in any of four different sets of measurement units as follows:

Set 1Standard USA

Set 2SI

Set 3Modified SI (HGB/MCHC in mmol/L, MCH in fmol)

Set 4Modified SI (HCT/PCT in %)

NOTE: The numeric values transmitted to the host are always sent in Standard USA units even if the unit of measure, also being transmitted, is Set 2, 3, or 4.

To convert the integer transmitted in the field corresponding to a particular parameter to the correct value for that parameter in the units being used, the decimal point must be moved leftward from its implied position to the right of the integer. The description of each field gives a shift count indicating how many places to move the decimal point in each case. If, for example, the decimal point is to be moved two places to the left, a field value of 00123 becomes 1.23. An integer representing the Units Set currently in effect is transmitted in the Units Set field (Section 7.3.46).

For Units Set 3, the HGB, MCH, and MCHC values must be multiplied by 0.6206 after the decimal point has been positioned.

The units of measure associated with the four sets are explained in the CELL-DYN 1800 Operators Manual.


7.3.1 WBC CountField 17

The WBC Count is in a numeric field five (5) characters long.

Units Set Shift Count Units Label

1 1 K/L

23 1 G/L

4 1 10E9/L

7.3.2 Spare FieldField 18

The Spare Field is a numeric field of five (5) characters. It has a constant value of zero (0) at this time, but is transmitted as five - (dashes) without surrounding double quotes, since it is a numeric field.

7.3.3 LYM CountField 19

The LYM Count is in a numeric field five (5) characters long.


1 1 K/L

23 1 G/L

4 1 10E9/L

7.3.4 MID CountField 20

The MID Count is in a numeric field five (5) characters long.


1 1 K/L

23 1 G/L

4 1 10E9/L

7.3.5 GRAN CountField 21

The GRAN Count is in a numeric field five (5) characters long.


1 1 K/L

23 1 G/L

4 1 10E9/L




7.3.7 RBC CountField 23

The RBC Count is in a numeric field five (5) characters long.


1 2 M/L

23 2 T/L

4 2 10E12/L

7.3.8 HGB ValueField 24

The HGB Value is in a numeric field, five (5) characters long.


1 1 g/dL

2 0 g/L

3 1 (x 0.6206) mmol/L

4 0 g/L

7.3.9 HCT ValueField 25

The HCT Value is in a numeric field five (5) characters long.


1 1 %

23 3 L/L

4 1 %

7.3.10 MCV ValueField 26

The MCV Value is in a numeric field five (5) characters long.


14 1 fL


7.3.11 MCH ValueField 27

The MCH Value is in a numeric field five (5) characters long.


1-2 1 pg

3 2 (x 0.6206) fmol

4 1 pg

7.3.12 MCHC ValueField 28

The MCHC Value is in a numeric field five (5) characters long.


1 1 g/dL

2 0 g/L

3 1 (x 0.6206) mmol/L

4 0 g/L

7.3.13 RDW ValueField 29

The RDW Value is in a numeric field five (5) characters long.


14 1 %

7.3.14 PLT CountField 30

The PLT Count is in a numeric field five (5) characters long.


1 0 K/L

23 0 G/L

4 0 10E9/L

7.3.15 MPV ValueField 31

The MPV Value is in a numeric field five (5) characters long.


14 1 fL


7.3.16 PCT Value*Field 32

The PCT Value is in a numeric field five (5) characters long.


1 2 %

23 1 mL/L

4 2 %

7.3.17 PDW Value*Field 33

The PDW Value is in a numeric field five (5) characters long.


14 1 10(GSD)

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S.



7.3.19 LYM % ValueField 35

The LYM % Value is in a numeric field five (5) characters long.


14 1 %

7.3.20 MID % ValueField 36

The MID % Value is in a numeric field five (5) characters long.


14 1 %

7.3.21 GRAN % ValueField 37

The GRAN % Value is in a numeric field five (5) characters long.


14 1 %



The Spare Field is a numeric field of five (5) characters. It has a constant value of zero (0) at this time, but is transmitted as five - (dashes) without the surrounding double quotes, since it is a numeric field.


The Spare Field is in a numeric field. It has not been implemented at this time, so it is always transmitted as a - single dash without the surrounding double quotes.

7.3.24 Spare FlagField 40

The Spare Flag is a numeric field of one (1) character. It has a constant value of zero (0) at this time, but is transmitted as a single - (dash) without the surround double quotes, since is presumed to be a spare numeric flag.

7.3.25 R4 WBC FlagField 41

The R4 WBC Flag is in a numeric field of one character. A value of 1 indicates that the flag is set, and a value of 0 indicates that it is clear.

7.3.26 GR3 WBC FlagField 42

The GR3 WBC Flag is in a numeric field of one character. A value of 1 indicates that the GRAN R3 flag is set, and a value of 0 indicates that it is clear.

7.3.27 MR3 WBC FlagField 43

The MR3 WBC Flag is in a numeric field of one character. A value of 1 indicates that the Mono R3 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.28 MR2 WBC FlagField 44

The MR2 WBC Flag is in a numeric field of one character. A value of 1 indicates that the Mono R2 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.29 LR2 WBC FlagField 45

The LR2 WBC Flag is in a numeric field of one character. A value of 1 indicates that the Lym R2 WBC flag is set, and a value of 0 indicates that it is clear.


The R1 WBC Flag is in a numeric field of one character. A value of 1 indicates that the R1 WBC flag is set, and a value of 0 indicates that it is clear.


The R0 WBC Flag is in a numeric field of one character. A value of 1 indicates that the flag is set, and a value of 0 indicates that it is clear.


7.3.32 Spare FlagsFields 48 through 60

There are thirteen Spare Flag fields which are each a numeric field of one (1) character. It has a constant value of zero (0) at this time. These are transmitted as follows (no surrounding quotes): ,,,,,,,,,,,,,

7.3.33 LRI FlagField 61

The LRI Flag is in a numeric field of two characters. A value of 16 indicates that the flag is set, and a value of 00 indicates that it is clear.

7.3.34 URI FlagField 62

The URI Flag is in a numeric field of two characters. A value of 32 indicates that the flag is set, and a value of 00 indicates that it is clear.

7.3.35 WBC Lower Meniscus TimeField 63

The WBC Lower Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds.

7.3.36 WBC Upper Meniscus TimeField 64

The WBC Upper Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds.

7.3.37 RBC Lower Meniscus TimeField 65

The RBC Lower Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds.

7.3.38 RBC Upper Meniscus TimeField 66

The RBC Upper Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds.

7.3.39 Recount RBC Lower Meniscus TimeField 67

The Recount RBC Lower Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds. It has a value of 0 if there was no recount.

7.3.40 Recount RBC Upper Meniscus TimeField 68

The Recount RBC Upper Meniscus Time is in a numeric field five (5) characters long. It gives the time in units of milliseconds. It has a value of 0 if there was no recount.

7.3.41 Limits SetField 69

The Limits Set is a numeric field of one character. It indicates which set of patient limits (1 to 4) was in effect for the given patient specimen.


7.3.42 Sample ModeField 70

The Sample Mode is an alphanumeric field of one character. An O (letter O) indicates that the specimen was taken in Open Sample mode; a P indicates Pre-Dilute mode.

7.3.43 RBC Metering Fault FlagField 71

The RBC Metering Fault flag is a numeric field of one character. A 0 (zero) indicates that there was no metering fault. A 1 (one) indicates a flow error, and a 2 (two) indicates a clog.

7.3.44 WBC Metering Fault FlagField 72

The WBC Metering Fault flag is a numeric field of one character. A 0 (zero) indicates that there was no metering fault. A 1 (one) indicates a flow error, and a 2 (two) indicates a clog.

7.3.45 Sampling Error or Other Sample Processing Fault Field 73

This is a numeric field of one character. A 0 (zero) indicates there was no error. A 1 (one) indicates there was an error.

7.3.46 Units Set FieldField 74

This is a numeric field of one character. The value is 1, 2, 3, or 4 as described in Section 7.3.

There are four sets of measurement units. A problem arises under the following conditions:

1. Some data is collected and stored under one unit of measure.

2. Subsequently, data is collected and stored under another unit of measure.

3. All the data is transmitted to a Laboratory Information System.

The numeric results data, starting in field 17, are always sent as Standard USA units even though that data may be displayed in a different unit of measure on the instrument. The displayed unit of measure, sent in field 74, is transmitted for informational purposes only and may or may not correspond to the Standard USA format of the transmitted numeric results data.

NOTE: A comma (,) follows the last field which immediately precedes the CRC characters.


TABLE 1 HISTOGRAM MESSAGES

Field # Description Start at

Byte Byte

Length Paragraph # N/A STX (0x02) 1 1 5.4.3 1 Message Type 2 3* 6.2 2 Instrument Type 8 7* 6.3 3 Serial Number 18 12* 6.4 4 Sequence Number 33 4 6.5 5 Specimen Type 38 2 6.6 6 Operator ID 41 3* 6.7 7 Specimen Date 47 8* 6.8 8 Specimen Time 58 5* 6.9 9 Specimen ID 66 16* 6.10

10 Specimen Name 85 16* 6.11 11 Specimen Sex 104 1* 6.12 12 Specimen DOB 108 8* 6.13 13 Dr Name 119 22* 6.14 14 Collect Date 144 5* 6.15 15 Collect Time 152 5* 6.16 16 Comment 160 16* 6.17

The above fields are common to both this Table and Table 3

17 Scale Factor (n/a) 179 5 7.2.1 18 Channel 1 Data 185 3 7.2.2 " " " " " ...

273 Channel 256 Data 1205 3 7.2.2 N/A CRC (2 hex ASCII printable) 1209 2 5.4.6 N/A ETX (0x03) 1211 1 5.4.7

*indicates characters enclosed in double quotation marks.

Note: If a field contains 3 characters plus double quotation marks and a comma, the total number of bytes used is 6. See the Histogram Message Example.


TABLE 2 HISTOGRAM RECORD DUMP

02 22 57 42 43 22 2C 22 20 43 44 31 37 30 30 22 . "WBC", "CD1800"

2C 22 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 22 2C , " ",

34 32 38 38 2C 30 30 2C 22 2D 2D 2D 22 2C 22 31 4288,00, " ","1

30 2F 31 39 2F 39 34 22 2C 22 31 36 3A 34 34 22 0/19/94", "16:44"

2C 22 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D , " " , 2D 2D 22 2C 22 20 20

20 20 20 20 20 20 20 20 20 20 20 20 20 31 22 2C 1",

22 4D 22 2C 22 20 20 2F 20 20 2F 20 20 22 2C 22 "M" , " / / " , "

20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20

20 20 20 20 20 20 22 2C 22 20 20 2F 20 20 22 2C " , " / ",

22 20 20 3A 20 22 22 C 22 20 20 20 20 20 20 20 " : " , "

20 20 20 20 20 20 20 20 20 22 2C 30 30 30 30 30 ",00000

2C 30 30 30 2C 30 30 30 2C 30 30 30 2C 30 30 30 ,000,000,000,000

2C 30 30 30 2C 30 30 30 2C 30 30 30 2C 30 30 30 ,000,000,000,000

2C 30 30 30 2C 30 34 32 2C 30 34 38 2C 30 35 34 ,000,042,048,054

2C 30 35 37 2C 30 35 39 2C 30 35 35 2C 30 34 35 ,057,059,055,045

2C 30 33 35 2C 30 32 35 2C 30 32 30 2C 30 31 36 ,035,025,020,016

...

... (channels have been deleted)

...

2C 30 35 32 2C 30 35 33-2C 30 35 31 2C 30 35 30 ,052,053,051,050

2C 30 34 39 2C 30 34 38-2C 30 34 37 2C 30 30 30 ,049,048,047,000

2C 30 30 30 2C 30 30 30-2C 30 30 30 2C 30 30 30 ,000,000,000,000

2C 36 35 03 ,65.

Note: Each line contains 16 characters starting with 1, not zero. The 02 and . bolded on the first line refer to the STX character. The 03 and 65 bolded on the last line refer to the ETX and CRC characters respectively.


TABLE 3 COUNT DATA MESSAGES

Field # Description Start at Byte Paragraph # n/a STX (0x02) 1 1 5.4.3 1 Message Type 2 3* 6.2 2 Instrument Type 8 7* 6.3 3 Serial Number 18 12* 6.4 4 Sequence Number 33 4 6.5 5 Specimen Type 38 2 6.6 6 Operator ID 41 3* 6.7 7 Specimen Date 47 8* 6.8 8 Specimen Time 58 5* 6.9 9 Specimen ID 66 16* 6.10

10 Specimen Name 85 16* 6.11 11 Specimen Sex 104 1* 6.12 12 Specimen DOB 108 8* 6.13 13 Dr Name 119 22* 6.14 14 Collect Date 144 5* 6.15 15 Collect Time 152 5* 6.16 16 Comment 160 16* 6.17 The above fields are common

to both this Table and Table 1

17 WBC Count 179 5 7.3.1 18 Spare Field 185 5 7.3.2 19 LYM Count 191 5 7.3.3 20 MID Count 197 5 7.3.4 21 GRAN Count 203 5 7.3.5 22 Spare Field 209 5 7.3.6 23 RBC Count 215 5 7.3.7 24 HGB Value 221 5 7.3.8 25 HCT Value 227 5 7.3.9 26 MCV Value 233 5 7.3.10 27 MCH Value 239 5 7.3.11 28 MCHC Value 245 5 7.3.12 29 RDW Value 251 5 7.3.13 30 PLT Count 257 5 7.3.14 31 MPV Value 263 5 7.3.15 32 PCT Value 269 5 7.3.16 33 PDW Value 275 5 7.3.17 34 Spare Field 281 5 7.3.18 35 LYM % Value 287 5 7.3.19 36 MID % Value 293 5 7.3.20 37 GRAN % Value 299 5 7.3.21 38 Spare Field 305 5 7.3.22 39 Spare Field 311 1 7.3.23


TABLE 3 COUNT DATA MESSAGES

(Continued)

40 Spare Field 313 1 7.3.24 41 R4 WBC Flag 315 1 7.3.25 42 GR3 WBC Flag 317 1 7.3.26 43 MR3 WBC Flag 319 1 7.3.27 44 MR2 WBC Flag 321 1 7.3.28 45 LR2 WBC Flag 323 1 7.3.29 46 R1 WBC Flag 325 1 7.3.30 47 R0 WBC Flag 327 1 7.3.31

4860 Spare Flags 329 1 7.3.32 61 LRI Flag 355 2 7.3.33 62 URI Flag 358 2 7.3.34 63 WBC Lower Meniscus Time 361 5 7.3.35 64 WBC Upper Meniscus Time 367 5 7.3.36 65 RBC Lower Meniscus Time 373 5 7.3.37 66 RBC Upper Meniscus Time 379 5 7.3.38

67 Recount RBC Lower Meniscus Time

385 5 7.3.39

68 Recount RBC Upper Meniscus Time 391 5 7.3.40

69 Limits Set 397 1 7.3.41 70 Sample Mode 399 1* 7.3.42 71 RBC Metering Fault Flag 403 1 7.3.43 72 WBC Metering Fault Flag 405 1 7.3.44

73 Sampling Error/Incomplete Aspiration Flag 407 1 7.3.45

74 Units Set Field 409 1 7.3.46 n/a CRC (2 hex ASCII printable) 411 2 5.4.6 n/a ETX (0x03) 413 1 5.4.7

* indicates characters enclosed in double quotation marks

Note: If a field contains 3 characters plus double quotation marks and a comma, the total number of bytes used is 6. See the Count Data Message Example.


TABLE 4 COUNT RECORD DUMP

02 22 20 20 20 22 2C 22-20 43 44 31 37 30 30 22 . " " , " CD1800"

2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 2D 2D 2D 22 2C , " ",

34 32 38 38 2C 30 30 2C-22 2D 2D 2D 22 2C 22 31 4288,00, " ","1

30 2F 31 39 2F 39 34 22-2C 22 31 36 3A 34 34 22 0/19/94", "16:44"

2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 2D 2D 2D 2D 2D , " " , " 2D 2D 22 2C 22 20 20

20 20 20 20 20 20 20 20-20 20 20 20 20 31 22 2C 1",

22 4D 22 2C 22 20 20 2F-20 20 2F 20 20 22 2C 22 "M" , " / / " , "

20 20 20 20 20 20 20 20-20 20 20 20 20 20 20 20

20 20 20 20 20 20 22 2C-22 20 20 2F 20 20 22 2C " , " / " ,

22 20 20 3A 20 20 22 2C-22 20 20 20 20 20 20 20 " : " , "

20 20 20 20 20 20 20 20-20 22 2C 30 30 35 32 35 ",00525

2C 2D 2D 2D 2D 2D 2C 30-30 30 31 30 2C 30 30 30 , ,00010,000

31 36 2C 30 30 34 39 39-2C 2D 2D 2D 2D 2D 2C 30 16,00499, ,0

30 32 35 31 2C 30 30 30-37 34 2C 30 30 32 32 36 0251,00074,00226

2C 30 30 39 30 30 2C 30-30 32 39 35 2C 30 30 33 ,00900,00295,003

32 37 2C 30 30 31 38 37-2C 30 30 33 39 35 2C 30 27,00187,00395,0

30 30 36 38 2C 30 30 30-32 37 2C 30 30 31 38 38 0068,00027,00188

2C 2D 2D 2D 2D 2D 2C 30-30 30 31 39 2C 30 30 30 , ,00019,000

33 31 2C 30 30 39 35 30-2C 2D 2D 2D 2D 2D 2C 2D 31,00950, ,

2C 2D 2C 30 2C 30 2C 30-2C 31 2C 31 2C 30 2C 30 ,,0,0,0,1,1,0,0

2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 2D 2C 2D 2C 2D ,,,,,,,,

2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 30 30 2C 30 30 ,,,,,,00,00

2C 30 34 38 33 37 2C 30-32 31 32 31 2C 30 36 34 ,04837,02121,064

31 31 2C 30 35 30 37 30-2C 30 30 30 30 30 2C 30 11,05070,00000,0

30 30 30 30 2C 34 2C 22-4F 22 2C 30 2C 30 2C 30 0000,4, "O",0,0,0

2C 31 2C 33 37 03 ,1,37.


HISTOGRAM MESSAGE EXAMPLE

Actual transmission [remarks for text here] (no CR/LF) STX [start of text] "WBC", [message type] Field 1 " CD1800", [instrument type] " ", [serial #] 1040, [sequence no.] 00, [specimen type] Field 5 "123", [operator ID] "08/31/03", [specimen date] "12:34", [specimen time] "123456789ABCDEFG", [specimen ID] " JOHN DOE", [specimen name] Field 10 "M", [specimen sex] "10/15/46", [specimen DOB] " DR JANE DOE", [doctor name] "08/30", [collect date] "09:50", [collect time] Field 15 " sample 1", [comment] Field 16 00000, [scale factor n/a] Field 17 000, [channel 1 data] Field 18 000, [channel 2 data] 000, [channel 3 data] 000, [channel 4 data] 000, [channel 5 data] 000, [channel 6 data]



(Continued)

000, [channel 7 data] 000, [channel 8 data] 000, [channel 9 data] 000, [channel 10 data] 000, [channel 11 data] 000, [channel 12 data] 000, [channel 13 data] 000, [channel 14 data] 000, [channel 15 data] 000, [channel 16 data] 000, [channel 17 data] 000, [channel 18 data] 000, [channel 19 data] 000, [channel 20 data] 000, [channel 21 data] 000, [channel 22 data] 000, [channel 23 data] 000, [channel 24 data] 000, [channel 25 data] 034, [channel 26 data] 034, [channel 27 data] 034, [channel 28 data] 034, [channel 29 data]



(Continued)

054, [channel 30 data] 054, [channel 31 data] 054, [channel 32 data] 054, [channel 33 data] 080, [channel 34 data] 080, [channel 35 data] 080, [channel 36 data] 080, [channel 37 data] 093, [channel 38 data] 093, [channel 39 data] 093, [channel 40 data] 093, [channel 41 data] 080, [channel 42 data] 080, [channel 43 data] 080, [channel 44 data] 080, [channel 45 data] 071, [channel 46 data] 071, [channel 47 data] 071, [channel 48 data] 071, [channel 49 data] 063, [channel 50 data] 063, [channel 51 data] 063, [channel 52 data]



(Continued)

063, [channel 53 data] 041, [channel 54 data] 041, [channel 55 data] 041, [channel 56 data] 041, [channel 57 data] 032, [channel 58 data] 041, [channel 59 data] 029, [channel 60 data] 029, [channel 61 data] 029, [channel 62 data] 029, [channel 63 data] 029, [channel 64 data] 027, [channel 65 data] 027, [channel 66 data] 027, [channel 67 data] 027, [channel 68 data] 027, [channel 69 data] 034, [channel 70 data] 034, [channel 71 data] 034, [channel 72 data] 034, [channel 73 data] 034, [channel 74 data] 039, [channel 75 data] 039, [channel 76 data]



(Continued)




(Continued)

034, [channel 245 data] 034, [channel 246 data] 034, [channel 247 data] 027, [channel 248 data] 027, [channel 249 data] 027, [channel 250 data] 027, [channel 251 data] 027, [channel 252 data] 027, [channel 253 data] 000, [channel 254 data] 000, [channel 255 data] 000, [channel 256 data] Field 273 [note comma after channel 256 but not after the checksum] E7 [checksum]

NOTE: Sample checksum value may not match this records data.

ETX [end of text]


COUNT DATA MESSAGE EXAMPLE

STX [start of text] Field 1 "WBC", [message type] " CD1800", [instrument type] " ", [serial #] 1040, [sequence no.] 00, [specimen type] Field 5 "123", [operator ID] "08/31/03", [specimen date] "12:34", [specimen time] "123456789ABCDEFG", [specimen ID] " JOHN DOE", [specimen name] Field 10 "M", [specimen sex] "10/15/46", [specimen DOB] " DR JANE DOE", [doctor name] "08/30", [collect date] "09:50", [collect time] Field 15 " sample 1", [comment] Field 16 00083, [WBC count] Field 17 , [spare field] 00015, [LYM count] 00006, [MID count] Field 20 00062, [GRAN count] , [spare field] 00476, [RBC count] Field 23



(continued)

00166, [HGB value] Field 24 00447, [HCT value] 00940, [MCV value] 00349, [MCH value] 00371, [MCHC value] 00118, [RDW value] 00230, [PLT value] Field 30 00084, [MPV value] 00019, [PCT value] 00174, [PDW value] , [spare field] 00178, [LYM% value] Field 35 00077, [MID% value] 00745, [GRAN% value] , [spare field] , [spare field] , [spare field] Field 40 0, [R4 WBC flag] 0, [GR3 WBC flag] 0, [MR3 WBC flag] 1, [MR2 WBC flag] 0, [LR2 WBC flag] Field 45 0, [R1 WBC flag] 1, [R0 WBC flag]



(continued)

, [spare field] Field 48 , [spare field] Field 49 , [spare field] Field 50 , [spare field] , [spare field] , [spare field] , [spare field] , [spare field] Field 55 , [spare field] , [spare field] , [spare field] , [spare field] , [spare field] Field 60 00, [LRI flag] 00, [URI flag] 04862, [WBC lower meniscus time] 02363, [WBC upper meniscus time] 08107, [RBC lower meniscus time] 07656, [RBC upper meniscus time] 00000, [recount lower time] 21845, [recount upper time] 1, [limits set] "O", [sample mode] Field 70 0, [RBC metering fault]



(continued)

0, [WBC metering fault] 0, [sample error/incomplete aspiration] Field 73 1, [units set field] Field 74 9B [checksum]

NOTE: Sample checksum value may not match this records data.

ETX [end of text]

Table of Contents1.0 Introduction1.1 Purpose1.2 Definitions and Conventions

2.0 Mechanical Interface2.1 Connector arrangement

3.0 Electrical Interface3.1 Voltage levels and electrical characteristics are as defined by the EIA RS-232C specification.3.2 The maximum recommended cable length is 30 meters, or 100 feet. The actual maximum workable cable length is dependent on the environment of the site, the selected baud rate, and the equipment being connected together.

4.0 Data Interface4.1 The asynchronous method of data transmission (serial by bit) is used.4.2 All information transmitted is in character form and is represented by 7-bit or ASCII.4.3 Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (least significant first), one or no parity bit, and one (1) or two (2) stop bits.4.4 Parity may be selected as none, odd, or even.4.5 The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per second (bps).4.6 With the exception of the control characters mentioned in Section 5.2, only printable ASCII characters (hex 20 to hex 7E) are used in a message.

5.0 Communication Protocol5.1 Communication Modes5.2 Transmission Control5.3 Response from Host5.4 Message Format

6.0 Identification (ID) Segment6.0.1 For counting purposes, the STX mentioned in Section 5.4.3 is taken as byte 1.

7.0 Results Segment7.1 The results for each specimen are sent in the format described. All numeric fields are integers, and some need to be scaled by the Host.7.2 Histogram Messages7.3 Count Data MessageTABLE 1 HISTOGRAM MESSAGESTABLE 2 HISTOGRAM RECORD DUMPTABLE 3 COUNT DATA MESSAGESTABLE 4 COUNT RECORD DUMPHISTOGRAM MESSAGE EXAMPLECOUNT DATA MESSAGE EXAMPLE

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07h7901_CELL-DYN 1800

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