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E-Seminar Protocols

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your phone line while listening

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One-Minute NMR2006 demo tour

• Chicago Area NMR Group: Madison, WI; Nov.11• KSU Functional Genomics Meeting: Manhattan, KS; Oct. 23-24• SMASH: Burlington, VT; Sept. 10 - 13• Am. Soc. of Pharmacognosy: Arlington, VA; Aug. 6 – 9• ENC: Monterey, CA; April 24 – 28

13 December 2006

Presented by Tim Peck &

Dean Olson

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One-Minute NMR2006 demo tour

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New Tools: New Scientific Advances

Chemical AssignmentRapid Dereplication via HPLC

Gronquist, Meinwald, Eisner, F. C. Schroeder, JACS 2005, 127, pp. 10810-10811

dqf-COSY spectrum 8 scans 512 complex incrementstotal time 3 h 45 min

coupled non-gradient HMQC spectrum 16 scans, 512 complex incrementstotal time 7 h

non-gradient HMBC spectrum baseline-subtracted in F164 scans, 460 complex incrementstotal time 21 h 45 min

NOESY spectrum 16 scans, 256 complex incrementstotal time 4 h 18 min

dqf-COSY

HMQC

HMBC

NOESY

S. Van der Sar, J. Blunt, and M. Munro, Org. Let. 2006, 8(10), pp. 2059-2061

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One-Minute NMR:Integrated System Approach

6- or 12-TrayCapacityfor Vialsor Trays

Multi-SolventWash Station

SampleReturn

Accident-FreeSyringeand Needle

Web-basedSoftware

Well Plate or VialSample Format

CapNMR Probe5- or 10-µL

DiscoveryTower

On-BoardInstrument

Server

• Microliter precision• 0.1 mm xyz positioning

Well Plate orVial Tray

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Automation: Accident-Free Syringe and Needle

Carriage comes to restbefore needle is lowered

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Automation: Solvent Conditioner

Solvent BottlesUnder Positive Pressure

Solvent ConditionerVacuum Pump

Result: Dry, Degassed, ProtectedDeuterated Solvent

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IntegratedApproach

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MicroFlow NMR Literaturewww.microNMR.com

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Your MicroFlow NMR Product Sourcewww.protasis.com

Technical Support:www.microNMR.com