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SURVEY OF BIOCHEMISTRYCourse Review

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PRS-1

• Catalytic efficiency is given by what expression?

1. kcat

2. kcat/KM

3. Vmax/KM

4. [E]T x kcat

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PRS-1

• Catalytic efficiency is given by what expression?

1. kcat

2. kcat/KM

3. Vmax/KM

4. [E]T x kcat

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PRS-2

• Which amino acid is an alpha-helix breaker?

1. Y2. W3. V4. P

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PRS-2

• Which amino acid is an alpha-helix breaker?

1. Y2. W3. V4. P

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PRS-3

• Amino acids are linked by what types of bonds in proteins?

1. Disulfide bonds2. Peptide bonds3. Both #1 and #24. None of the above

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PRS-3

• Amino acids are linked by what types of bonds in proteins?

1. Disulfide bonds2. Peptide bonds3. Both #1 and #24. None of the above

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PRS-4

• Which cofactors are required for the aldolase reaction?

1. NAD+

2. CoA3. FAD4. None

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PRS-4

• Which cofactors are required for the aldolase reaction?

1. NAD+

2. CoA3. FAD4. None

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PRS-5

• Which of the following is NOT an example of a cofactor?

1. Glucose2. Thiamine Pyrophosphate3. Pyridoxal Phosphate4. Coenzyme A

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PRS-5

• Which of the following is NOT an example of a cofactor?

1. Glucose2. Thiamine Pyrophosphate3. Pyridoxal Phosphate4. Coenzyme A

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PRS-6

• What function does glucagon exhibit?

1. Increasing glycogen breakdown2. Binding to receptors on liver cells3. Triggering increased levels of cAMP

production4. All of the above

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PRS-6

• What function does glucagon exhibit?

1. Increasing glycogen breakdown2. Binding to receptors on liver cells3. Triggering increased levels of cAMP

production4. All of the above

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PRS-7

• Pyruvate carboxylase converts ___ to _____.

1. Acetyl CoA to pyruvate 2. Pyruvate to acetyl Coa3. Pyruvate to Oxaloacetate4. Pyruvate to Phosphoenolpyruate

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PRS-7

• Pyruvate carboxylase converts ___ to _____.

1. Acetyl CoA to pyruvate 2. Pyruvate to acetyl Coa3. Pyruvate to Oxaloacetate4. Pyruvate to Phosphoenolpyruate

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PRS-8

• A point mutation in which a purine is replaced by a pyrimidine is called:

1. Transversion2. Transition3. Insertion4. Deletion

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PRS-8

• A point mutation in which a purine is replaced by a pyrimidine is called:

1. Transversion2. Transition3. Insertion4. Deletion

See Section 25-4

From Reading - not mentioned in

lecture…

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PRS-9

• Which statement is true?1. Hemoglobin binding of CO2 stabilizes the T

form

2. The sickle cell form of hemoglobin contains a point mutation (E->V)

3. 2,3-Bisphosphoglycerate decreases hemoglobin’s affinity for O2

4. All of the above

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PRS-9

• Which statement is true?1. Hemoglobin binding of CO2 stabilizes the T

form

2. The sickle cell form of hemoglobin contains a point mutation (E->V)

3. 2,3-Bisphosphoglycerate decreases hemoglobin’s affinity for O2

4. All of the above

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PRS-10

• Which of the following is a unit of O2 pressure?

1. mm Hg2. mol/L3. %4. nm

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PRS-10

• Which of the following is a unit of O2 pressure?

1. mm Hg2. mol/L3. %4. nm

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PRS-11

• See Figure 1. The shift from blue to red indicates:

1. An increase in pH

2. A decrease in pH

3. An increase in hemoglobin concentration

4. A decrease in hemoglobin concentration

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PRS-11

• See Figure 1. The shift from blue to red indicates:

1. An increase in pH

2. A decrease in pH

3. An increase in hemoglobin concentration

4. A decrease in hemoglobin concentration

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PRS-12

• Which are 5-C monosaccharides?

1. Glucose and ribose

2. Ribose and fructose

3. Ribose and mannose

4. None of the combinations above

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PRS-12

• Which are 5-C monosaccharides?

1. Glucose and ribose

2. Ribose and fructose

3. Ribose and mannose

4. None of the combinations above

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PRS-13

• Which reaction is an example of an acid-base catalyzed reaction?

1. Hexokinase2. Pyruvate kinase3. Phosphoglucose isomerase4. Pyruvate dehydrogenase

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PRS-13

• Which reaction is an example of an acid-base catalyzed reaction?

1. Hexokinase Nucleophilic attack2. Pyruvate kinase Nucleophilic attack3. Phosphoglucose isomerase4. Pyruvate dehydrogenase

5-step Reaction

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PRS-14

• Which of the following is not a step in the pyruvate dehydrogenase mechanism?

1. Decarboxylation2. Reduction of FAD to FADH2

3. Oxidation of NADH to NAD+ 4. Production of acetyl-CoA

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PRS-14

• Which of the following is not a step in the pyruvate dehydrogenase mechanism?

1. Decarboxylation2. Reduction of FAD to FADH2

3. Oxidation of NADH to NAD+ 4. Production of acetyl-CoA

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Pyruvate Dehydrogenase Rxn

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PRS-15

• Which of the following is not a disulfide reducing agent?

1. Cysteine2. Beta-mercaptoethanol3. Lipoic acid4. Linoleic acid

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PRS-15

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PRS-16

• cDNA _____.

1. Is DNA found in the cytosol of a cell.

2. Comes from reverse transcription of mRNA.

3. Stands for “capped” DNA.

4. Contains the DNA sequence from the entire genome.

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PRS-16

• cDNA _____.

1. Is DNA found in the cytosol of a cell.

2. Comes from reverse transcription of mRNA.

3. Stands for “capped” DNA.

4. Contains the DNA sequence from the entire genome.

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PRS-17

• Which are nonpolar amino acids?

1. Gly, Val, Lys

2. Arg, Leu, Met

3. Ile, Pro, Phe

4. Ala, Met, Ser

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PRS-17

• Which are nonpolar amino acids?

1. Gly, Val, Lys

2. Arg, Leu, Met

3. Ile, Pro, Phe

4. Ala, Met, Ser

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PRS-18

• Beta sheets can contain _____.

1. Antiparallel strands

2. Parallel strands

3. Interstrand H-bonds

4. All of the above.

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PRS-18

• Beta sheets can contain _____.

1. Antiparallel strands

2. Parallel strands

3. Interstrand H-bonds

4. All of the above.

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PRS-19

• The hydrophobic effect is driven by ______.

1. Enthalpy2. Entropy3. The concentration of water4. Temperature

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PRS-19

• The hydrophobic effect is driven by ______.

1. Enthalpy2. Entropy3. The concentration of water4. Temperature

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PRS-20

• Which is a major component of brain lipids?

1. Fatty acids2. Triglycerides3. Gangliosides4. Phospholipids

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PRS-20

• Which is a major component of brain lipids?

1. Fatty acids2. Triglycerides3. Gangliosides4. Phospholipids

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Reminders

• Final exam is closed-book.• Bring a calculator.• Study by:

– Reading the text– Reviewing suggested HW problems– Reviewing What You Should Know

summaries (no more added)– Reviewing past exams– Studying lecture notes

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CETL Course Survey(s)

• Course-Instructor Opinion Survey (CIOS)– http://www.coursesurvey.gatech.edu– Also on T-Square Home Page

• Pilot Survey– Go to T-Square: Announcements for link to

survey– Focus groups to learn more about student

opinions – Deadline: August 3, 2008– IRB Approval requested: minimal risk