A B DC

E F HG

10 minutes

30 minutes

KSOMaacontrol

(260 mOsm)

KSOMaa +10% glycerol(1800 mOsm)

KSOMaa +1.4 M sucrose(1800 mOsm)

No primary control

K L NM

O P RQ

15 minutes

30 minutes

KSOMaacontrol

(260 mOsm)

KSOMaa +1.4% glycerol(460 mOsm)

KSOMaa +0.2 M sorbitol(460 mOsm)

No primary control

I J

S T