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S OAIRSTREAM CLASS II BIOHAZARD SAFETY CABINETQUICK REFERENCE SHEET

Installation Instructions

1. Refer to Packaging Contents(refer to section 2.2) and ensure all items are received.

2. The Biohazardsticker provided with the manual may be pasted at the entrance of the laboratory.

3. The Log Record, at the end of the manual, should be started.

4. If no pathogenic material is used in the cabinet, this should be recorded inside the Log Record. In this case, nodecontamination will be necessary before filter changing.

5. Choose a suitable location for your cabinet (refer to section 3.1). This is important in order to ensure minimal external

airflow disturbances which can affect cabinet airflow and containment.

6. The screws securing the counterweight (behind the cabinet) for shipment should be removed before the sash can be

moved. After this step, move the sash to the normal operating height.

7. Connect the cabinet to the main power supply with the supplied power cable. The fan is automatically switched on. PLS

CHECK PANEL message is displayed; please check that all LEDs and buzzer are on for 6 seconds.

8. The cabinet must be certified (refer to section 3.4) after it is installed, and before it is first used.

General / Safety Instructions

1. The use of any hazardous material in the cabinet requires that it be monitored by an industrial hygienist, safety officer, or

other qualified individual. Explosive or flammable substances should never be used in the cabinet unless a qualified safety

professional has evaluated the risk. If chemical, radiological or other non-microbiological hazards are present, be sure to

employ appropriate protective measures.

2. This cabinet is suitable for agents classified under biosafety levels 1 to 3. Refer to Appendix A for more information on

biosafety levels. It should not be used with any agents of an unknown nature and / or extremely hazardous materials.

3. Read all instructions before proceeding and observe the installation procedure and environmental / electrical requirements.

4. Regular maintenance, in accordance with chapter 5 of this manual, should be carried out.

5. Do not use any disinfectant containing chlorine-based substances when cleaning the cabinet as they may cause corrosion

of the stainless steel surfaces.

6. In this manual, important safety related points will be marked with this symbol:

Starting the Cabinet

1. Prepare a written checklist of materials / apparatus necessary for work in the cabinet. Turn off the UV lamp (if it is being

used). Turn on the blower and raise the sash (default FAN PIN is 0001). Leave the blower on for 3 minutes (warm-up timer

the default duration is 3 minutes, however, this may be adjusted refer to section 4.5.1.2).

2. Wash hands thoroughly using germicidal soap. Wear gloves for hand protection (double gloving may be employed) and

put on a clean long-sleeved laboratory coat. Adjust your seating position so that your face is above the work zone opening.

Never use the cabinet to store supplies or laboratory equipment. Placing excessive materials in the work zone

may affect the airflow and containment capabilities of the cabinet.

Do not block the front and back air grilles.

Avoid use of toxic, explosive, or flammable substances within the cabinet unless the use has been specifically

approved by a safety professional.

Never operate the cabinet when any alarms are activated.

Minimize the use of syringes or other sharp objects within the cabinet.

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S OAIRSTREAM CLASS II BIOHAZARD SAFETY CABINETQUICK REFERENCE SHEET

3. Open the sash window to its maximum position. Thoroughly surface-decontaminate the work surface, side walls, back wall,

and internal surface of the window using 70% ethanol (or other disinfectant depending on materials used in the cabinet).

4. Surface-decontaminate all materials / apparatus before loading them into the work zone.

5. Load and arrange the materials/apparatus to minimize the movement of contaminated items over clean items by

segregating the contaminated items from the clean items into two equal areas, one on the left, and one on the right to

provide best protection against cross contamination.

6. Adjust the sash to the normal operating height and allow the blower to run for another 3 minutes in order to purge the work

zone of contaminants. Minimize room activity (personnel movements, closing and opening of doors, etc.) as these external

airflow disturbances may affect cabinet airflow and containment.

Working in the Cabinet

1. Work as far into the cabinet as possible, at least 150mm/6 inches back from the front air intake grille. Hold open tubes and

bottles horizontally to avoid spillage. Discard empty tubes and bottles into collection bags inside the cabinet immediately.

Surface-decontaminate before removing any potentially contaminated items from the interior.

2. Work in the cabinet in a slow and controlled manner. Move arms in and out of the work zone opening slowly and in the

direction perpendicular to the plane of the work zone opening. The rapid movement of arms in a sweeping motion may

disrupt the air barrier and induce contaminants out of the cabinet.

3. In case of spillage, use absorbent pads or towels to cover the spillage, and then soak it with 10% bleach or another

disinfectant. Wait for 10 to 15 minutes before cleaning the affected and surrounding area (and equipments) with an

appropriate disinfectant. Decontamination (with formaldehyde) may be necessary after a spillage where inaccessible

surfaces may be contaminated.

4. When the sash is fully closed, the blower should be turned off to prevent overheating. NB: the FAN or ADMIN PIN is

required to turn the blower off. This is a safety feature to prevent unauthorized personnel from shutting down the cabinet.

Shutting Down the Cabinet

1. It is recommended that the cabinet be operated continuously whenever possible to ensure containment and cleanliness.

NB: airflow studies have shown that air from the cabinet may escape after the fan has been turned off, due to thermal

currents from the interior of the cabinet. If the user chooses to turn the cabinet off at the end of a work session, the

following procedures are recommended.

2. Leave the blower on for 3 minutes in order to purge airborne contamination from the work area. Discard all biohazard

waste into the biohazard bags (including the outer gloves if double gloving was employed). Seal biohazard bags and

dispose properly (waste should be autoclaved, if appropriate).

3. Surface-decontaminate all materials / apparatus and remove them from the cabinet. Thoroughly surface-decontaminate the

work surface, side walls, back wall, and internal surface of the window using 70% ethanol (or other disinfectant depending

on materials used in the cabinet).

4. Remove laboratory coat and gloves and wash hands thoroughly with germicidal soap. Turn off the blower and close the

sash. The UV lamp, if installed, may be turned on at this stage.

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S O AIRSTREAM CLASS II

BIOHAZARD SAFETY CABINET

USER & SERVICE MANUAL

S OEsco Manual Revision No. AC2.v2.05.07.2004

Specifications subject to change without notice

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WELCOME NOTE FROM ES OCongratulations! You have just purchased one of the finest biohazard safety cabinets available.

It is a pleasure to welcome you to the growing number of customers who own and operate Esco

biological safety cabinets. For your convenience all exposed exterior and interior surfaces of this

products has been decontaminated and wiped down in a controlled clean environment, protective

coverings on stainless steel and plastic surfaces, where present have also been removed.

Your new Esco Airstream Class II cabinet includes many unique features and exciting

improvements to simplify maintenance, enhance safety and improve ergonomics. These include:

A new user-friendly SentinelTM microprocessor-based control and alarm system whichsimplifies operation and enhances user safety

ULPA filters efficient to >99.9999% for better operator and product protection Completely frameless sliding window for maximum visibility into the work zone Generously sized drain pan to contain any liquid spills and provide greater cleanability Work zone lighting with an intensity above 1000 lux / 94 foot candles using 5000k fluorescent

lamps for greater comfort and less glare

A backward curved motorized impeller system which distributes air within the positive plenumin all directions to provide lower noise levels, better airflow uniformity, and lower power

consumption

A retrofit system that allows optional accessories to be easily fitted later in the field

Please read this manual to familiarize yourself with the proper installation, operation, and

maintenance of the cabinet. For pharmaceutical customers, additional IQ/OQ/PQ documentation

is available on request.

We recommend that this manual, together with the factory test report, be kept near the cabinet for

convenient reference by operators and qualified maintenance personnel

If you have any questions that are not addressed in this manual, please do not hesitate to reach

us by e-mail at [email protected] or give us a call at +65 65420833.

With warm regards from Singapore,

21 Changi South Street 1 Singapore 486777 +65 65420833 FAX+65 65426920Visit our website at www.escoglobal.com

Lim Lay Yew

Chief Executive Officer
mailto:[email protected]:[email protected]

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21 Changi South Street 1 Singapore 486777 PHONE +65 65420833 FAX +65 65426920E-MAIL [email protected] WEBSITE www.escoglobal.com

Table of Contents1

TABLE OF CONTENTS

Table of Contents...................................................................................... 1

Safety Warning and Limitation of Liability............................................. 4

Declaration of Conformity.................................................................................................. 5

Freight Claim Information......................................................................... 6

Warranty Terms and Conditions.............................................................. 8

Corporate Profile Biotechnology Equipment Division.......................... 9

A. USER SECTION

I. Basic Product Information................................................................. .. 11

II. Unpacking Your Cabinet.................................................................... .. 15

2.1 Step-by-Step Procedure........................................................... 15

2.2 Packaging Contents.................................................................. 18

III. Installing Your Cabinet...................................................................... 19

3.1 Choosing a Suitable Location................................................... 19

3.2 Environmental/Electrical Condition........................................... 28

3.3 Installing Your Cabinet ............................................................. 29

3.4 Performance Validation/Certification............................................ 30

3.4.1 The importance of performance validation/certification...... 31

3.4.2 Recertification..................................................................... 313.4.3 Disclaimer........................................................................... 31

3.4.4 References for Qualified Certifiers...................................... 31

IV. Operating Your Cabinet..................................................................... 32

4.1 Sentinel Control System......................................................... 32

4.1.1 Software Self-Reset.. 35

4.1.2 Hardware Reset. 35

4.2 Starting the Cabinet................................................................................ 36

4.3 Working in the Cabinet............................................................. 38

4.4 Shutting Down the Cabinet................................................... ... 40

4.5 Menu Options........................................................................... 41

4.5.1 Settings........................................................................................................ 42

4.5.1.1 Set Time.. 42

4.5.1.2 Warm Up. 42

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Table of Contents2

4.5.1.3 Post Purge.. 42

4.5.1.4 UV Timer. 43

4.5.1.5 Air Velocity Unit Selection.... 43

4.5.2 Calibration.. 44

4.5.2.1 Set Constant.. 44

4.5.2.2 Zero Sensor.. 44

4.5.2.3 Calib Sensor.. 44

4.5.3 Admin. 44

4.5.3.1 New Admin PIN. 45

4.5.3.2 New Fan PIN.. 45

4.5.3.3 A/F Monitor. 46

4.5.3.4 Reset B/H/M.. 46

4.5.4.5 Reset UV/H/M... 464.5.3.6 Reset Default. 47

4.5.4 Set Mode 47

4.5.4.1 Normal Mode. 47

4.5.4.2 Maintenance Mode 47

4.6 Ergonomics............................................................................... 48

V. Maintaining Your Cabinet.................................................................. 49

5.1 Maintenance Schedule........................................................... 49

5.2 Decontamination....................................................................... 50

5.3 Disinfecting Agents.. 50

B. SERVICE SECTIONI. Maintenance by Service Personnel.......................................................................... 52

1.1 Calibration of Sentinel Control.................................................. 52

1.1.1 Maintenance Mode... 52

1.1.2 Calibration Menu... 54

1.1.2.1 Zero Sensor 54

1.1.2.2 Calib Sensor... 55

1.1.3 Final Checking in Normal Mode. 57

1.2 Software Update.................................................................................. 59

1.3 Cabinet Airflow Balancing.............................................................................. 60

1.4 Filter Changing................................................................................................... 60

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Table of Contents3

1.5 Recertification........................................................................... 62

1.6 Decontamination Procedure........................................................................... 64

II. Product Specification.......................................................................... 74

2.1 Engineering Details........................................................................................ 74

2.2 Optional Retrofit Kits................................................................. 74

2.3 Technical Specification................................................................................... 74

2.4 Electrical Diagram.......................................................................................... 74

2.5 Sentinel Control System................................................................................. 75

III. Troubleshooting ...................................................................................................... 76

3.1Visual Troubleshooting....................................................................................... 76

3.2 Electrical Troubleshooting.............................................................................. 76

3.3 Software Troubleshooting... 79

Copyright Information............................................................................. 80Warranty Registration and Survey Form.................................................................... 81

Defect Reporting Form............................................................................ 84

Replacement Parts List........................ 85

APPENDIXES

Appendix A

Biosafety Levels Classification. 91

Appendix B

Introduction to Biohazard Safety Cabinet........................................... 92

Appendix C

KI-Discus Test....................................................................................... 98

Appendix D

UV Lamps in Laminar Flow and Biological Safety Cabinet................ 102

Appendix E

Exhaust Ducted Class II Type A2 Safety Cabinets................................ 107Appendix F

Reference Materials............................................................................... 113

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Declaration of Conformity*In accordance with EN 45014:1998

We Esco Micro Pte Ltd

of 21 Changi South Street 1

Singapore 486777Tel: +65 65420833Fax: +65 65426920

hereby declare on our sole responsibility that the product:

Model : Airs tream Class II Biohazard Safety Cabinet

has been designed and manufactured to conform with the following standards,when installed and used in accordance with the operating and maintenance manual:

EN 50081 - 1 : 1992 Emissions EN 55022 Class A Conducted Emissions EN 61000 - 3 - 2 / 3 Harmonic Current Emissions and Voltage Fluctuations EN 55022 Class A Radiated Emissions EN 50082 - 1 : 1997 Immunit y EN 12469 : 2000 Microbiological Safety Cabinets

EN 61010 1 (1997) Electrical Equipment Safety Requirements

This declaration is valid also as declaration of conformity to the following directives:

73/23/ EEC Directive on low voltage 89/336/EEC Directive on electromagnetic compatibi lity 93/68/EEC Direct ive on CE Marking 98/37/EEC Directive on machinery

More information may be obtained from Escos authorised distributors located within the EU and a list of theseparties and their necessary contact information is available on request from Esco.

Lim Lay YewChief Executive Officer

* Applicable only to 230V 50 Hz cabinets

Declaration of Conformity 5

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Freight Claim Information 6

FREIGHT CLAIM INFORMATION

Esco inspects each product for defects before shipment. Esco products are then carefully packed in

compliance with carrier regulations and thoroughly inspected before leaving our plant. Responsibility for their

safe delivery is assumed by the carrier upon acceptance of the shipment. Occasionally damage occurs in

transit. Claims for loss or damage sustained in transit must be made upon the carrier.

Please remember that you are responsible for all freight claims and the cost of all replacement

pieces for each shipment you accept. Inspect each shipment very carefully before acceptance.

Carefully inspect each pallet or crate upon arrival. If a shipment is found to be damaged upon

delivery, be sure to have the driver/carrier note all damage details on the delivery receipt. This is essential or

your claim may be denied. Also if pallets are stacked, please note "Stacked Pallets" on the delivery receipt

(pallets are not stacked when shipped, unless otherwise stated for certain products). Esco is not responsible

for pallets stacked at carrier terminal. Any unloading difficulties or damages due to stacked materials are

carrier's responsibility.

If freight damage is discovered, please refer to the following guidelines in order to process and

effective freight claim:

ACCEPTED FREIGHT WITH NOTED/VISIBLE LOSS OR DAMAGE

Any external evidence of loss or damage must be noted on the freight bill or delivery receipt and signed

by the courier's agent or delivery driver

(NOTE: Failure to properly describe evidence of loss or damage may result in the carrier refusing to

honour a claim)

Contact delivering terminal to arrange for a claim form and inspection report to be faxed or mailed to you

Notify Esco Micro Pte Ltd regarding which items need replacement

Keep all damaged items and packing material until claim is resolved between you and the carrier

ACCEPTED FREIGHT WITH CONCEALED LOSS OR DAMAGE

When a damage or loss is discovered during unpacking:

Contact the carrier immediately upon discovery of damage and request for inspection by the carrier's

agent.

Carrier will determine inspection needs based on value and time elapsed

Notify Esco regarding which items require replacement

Esco will fax to you an order acknowledgment that includes value of items for claim (less freight amount)

Esco will invoice you for replacement materials

Esco standard payment terms will apply

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Freight Claim Information 7

UNACCEPTED FREIGHT

If substantial damage is noted upon inspection you have the right to refuse part or all of a shipment. Do

not unpack pallets or crates with damaged materials. Individual items cannot be refused. You must

refuse the entire pallet or accept the freight with noted damage (see above)

Esco will handle all freight claim procedures and process a replacement order for your company for the

damaged pieces at no charge. (if the original order was shipped under CIF terms i.e. Esco had covered

the insurance)

NOTE: Any correspondence with Esco regarding loss or damage must be accompanied by a copy of the

shipping carrier's report. Esco will not accept returns that have not been authorized.

In the event of accepted freight with damage or loss, notification of loss or damage must be sent to

the carrier within 10 days of receiving the freight. Notification outside the 10-day time frame may

result in shipping damage claim being denied.

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WARRANTY TERMS AND CONDITIONS

Esco warrants that this equipment will perform according to the specifications for 3 years from the

date of purchase. With exceptions noted below *, this 3 years warranty applies to all parts and components

of this equipment (* 3 years except consumable items filter, light tubes, UV tubes, pre-filters)

Esco's liability under this warranty shall be limited to repair or replacement of the equipment at

Esco's option, and under no circumstances shall Esco be liable for the equipment damage arising out of or in

any way connected with the failure of the equipment to perform as set forth herein. This limited warranty is in

lieu of all other expressed or implied warranties, including those of merchantability and fitness for a particular

purpose.

In the event of a defect, a malfunction or failure during the warranty period, Esco will repair or

replace, at its option, the product or component therein which upon examination by Esco shall appear to be

defective, or not up to factory specifications. The purchaser must pay any transportation or labour expenses

incurred in removing and returning the product to the service centre.

Esco cannot be held responsible for malfunctions, damage to people or property to non-compliance,

poor or no maintenance or improper use of the cabinet.

This warranty shall not apply to any Esco product or part thereof which has been subject to misuse,

abuse, accident, shipping damage, improper installation or service and testing, or damage by fire, flood, or

acts of God. If the serial number of this product is altered, removed or defaced as to be illegible, the warranty

shall be null and void in its entirety.

The use of non-Esco parts and/or parts not supplied directly by Esco or our authorized distributors,

including but not limited to maintenance parts, spare parts, replacement parts, system components and/orsystem accessories, shall void all expressed or implied warranties.

To obtain warranty service, the defective product or part(s) must be returned to Esco or an

authorized Esco Factory Service Centre along with a proof of purchase. It is mandatory, upon purchase,

for the user to register receipt of the equipment with Esco online at www.escoglobal.com.

Al ternat ively, the user can also complete the warranty registration form which can be found with in

this manual on page 81.

Please take note that when returning the defective product or part(s), you must first obtain a return

authorization (RA) number from Esco and it must be noted on the documents accompanying the return. The

defect reporting form can be found on page 84.

Warranty Terms and Conditions 8

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CORPORATE PROFILE BIOTECHNOLOGY EQUIPMENT DIVISION

A History of Clean Air Device

Manufacturing Since 1978

In 1978, Esco was foundedto provide clean air solutions for the

emerging high-tech industrial and

life sciences industries. During

those early years, our expertise with

clean air technology was also

applied towards the construction of

laminar flow clean air devices.

Biohazard safety cabinets

(today a core offering of Esco

Biotech) and fume hoods were also

designed and manufactured during

the early 1980s thus expanding the

companys scope in the field of

containment technology. That same

period also saw the first exports of

locally developed and proven clean

air technology by Esco. In 1985, in-

house sheet metal fabrication

capabilities were established.

Eventually, the

Biotechnology Equipment Division

was formed to focus on the design

and manufacturing of laminar flow,

biohazard safety and other HEPA-

filtered cabinets for the laboratory.

Our unique background in industrial

cleanroom and contamination

control technology differentiates us

from our competitors who are solelylaboratory equipment suppliers. The

division draws from the broad base

of resources of the group which is

also engaged in other fields of cleanair equipment technology.

Today, more than 20,000

Esco laminar flow, biohazard safety

and other clean air cabinets are in

use in the field with some

installations dating back to the

1980s still in operation.

Esco supplies many high-

tech industries with critical

equipment that help keep products,

people, and processes free from

contamination. For example, our

cleanroom construction components

help pharmaceutical manufacturers

meet GMP standards, keeping the

drugs they produce clean. Our

laminar flow mini-environments help

microelectronics manufacturers

increase product reliability and

yields. In the laboratory,

researchers use our safety cabinets

for clinical biological research in

order to develop vaccines for

dangerous diseases. In a small

way, Esco contributes towards

these essential technologies that

have become the backbone of our

modern lives.

The Esco Biotechnology

Equipment Division is a highly

focused manufacturer of laminar

flow, biohazard safety and other

HEPA-filtered cabinets for the

laboratory with a history of quality

cabinets since 1978. We are

predominantly oriented towards the

international marketplace, with

sales in more than 60 countries. Anetwork of international distributors

and partners allows us to provide

quick and responsive local service

and support.

Sophisticated Production

Capabilities

Today, Escos production

capabilities for our laboratory

equipment products are centralized

in a 3,900 m 2 / 42,000 sq. ft facility

separate from our Singapore

corporate headquarters.

This state-of-the-art

fabrication centre houses the latest

CNC (computer numerically

controlled) sheet metal machinery

and is highly automated to ensure

that all products produced are of a

high quality with less human

intervention. An advanced powder-

coating process is also operated in-

house to ensure an aesthetic finish

on all Esco laboratory equipment

products. Esco is committed to

making continuous investments in

fabrication technologies to ensure

superior quality and total customer

satisfaction.

Corporate Profile Biotechnology Equipment Division 9

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World-Class Research and

Development

At Esco, continuous

research and development is a

corporate policy realized through

tangible investments in personnel,

equipment and facilities. Currently,

1 out of every 2 non-production staff

at Esco works in a research and

development position.

Visitors are welcome to visit

the Esco Biotech Equipment

Technology Centre located in

Singapore at our worldwide

headquarters, which features a

demonstration laboratory,

conference / training facilities for

visitors, and various testing

laboratories in which state-of-the-art

research and tests are continuously

conducted into improving the

performance of our products.

The laboratories are

equipped with the latest

instruments, properly calibrated and

maintained: particle counters, air

velocity meters, flow visualizationdevices, KI discus testing

apparatus, electrical safety

analysers, light/noise level/vibration

meters and

microbiological/containment test

instruments.

ISO 9001 Quality Management

System

At Esco, quality and

customer satisfaction are our top

priorities, which is why the company

operates under an ISO 9001

certified quality management

system. Extensive quality control

and testing is carried out at all steps

of the design and production

process.

ISO 14001 Environmental

Management System

Esco also practices

responsible corporate citizenship by

demonstrating a firm commitment tothe environment. Many Esco

products are designed for low

energy consumption, while our

cabinets are also constructed of

easily recyclable stainless steel.

Commitment to the Global

Marketplace

Esco regularly participates

in numerous major international,

regional and national industry trade

shows and exhibitions, both directly

and indirectly with the regional

distributors. For the updates on

Escos upcoming events and

exhibitions, visit our website

section:

http://biotech.escoglobal.com/trades

hows.htm

Corporate Profile Biotechnology Equipment Division 10

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USER SECTION

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CHAPTER I

BASIC PRODUCT INFORMATION

Class II cabinets provide product, operator and environment protection. They are suitable for general

microbiological work with agents assigned to biosafety levels 1, 2, or 3. (For more details please refer to

Appendix A and Appendix B) Class II cabinets are recommended for most applications and are the most

common and cost-effective systems available on the market today.

AIRFLOW PATTERN INSIDE THE CABINET

Air flows from the room into perforations located at the front of the work zone (in order to prevent

contamination of the product, the inflow does not mix with the clean air present in the actual work zone of the

cabinet), and then travels through an air return path below the work surface.

Inflow

Downflow(Approx. 65%)

HEPA-filtered exhaust(Approx. 35%)

An H14 HEPA/ULPA-filtered vertical laminar flow air stream within the cabinet (also referred to as the

downflow) moves downwards from the top of the work zone towards the work surface.

Close to the work surface, the downflow air stream splits with a portion entering perforations towards the

front and back of the work zone. Combined with the inflow, air is moved within the cabinet, through an air return

path (underneath the work surface, and behind the back wall) to a plenum in which the blower system is mounted.

Chapter I Basic Product Information

USER SECTION

11

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From the common air plenum, approximately 30% of the air is H14 HEPA/ULPA-filtered and exhausted

(thus protecting the operator and the environment from exposure to biological hazards), while approximately 70% is

H14 HEPA/ULPA-filtered and re-circulated as the downflow

SAFETY

At Esco, your safety means the world to us. This vision is fully realized in the Airstream Class II with many

features that really bring your safety to the next level.

Shaped inflow air grille, combined with the raised front armrest, eliminates the possibility of the operator

blocking the air perforations. (which may compromise safety)

Latest minipleat seperatorless H14 HEPA/ULPA filters operating at a typical efficiency of 99.9999% at MPPS,

0.3 and 0.12 microns for best product and operator protection.

Fail-safe double balancing system is employed in the event that a cable should fail the sash will tilt and lock in

position so as not to endanger the operator

Easily accessible integrated paper catch in the air return area in order to prevent wipes and other materials from

being drawn into the blower system Dynamic Chamber

TM plenum design surrounds contaminated areas with negative pressure plenums thus

preventing possible filter seal/gasket and cabinet carcass leaks

All electrical components are UL listed or UL recognized

Audible and visual alarms for unsafe sash positions

Sash glass must be fully closed before UV lamp will activate

Red colour-coded access panels to electrical system and filter/blower internal plenum to caution users of the

potentially dangerous/contaminated parts

Automatic pre-purge and post purge cycles ensure correct cabinet operation and operator safety.

Permanent steel plenum which is less prone to leaks and resistant to decontamination agents

Electro galvanized steel sheets utilized for cabinet outer shell for greater rust resistant properties

Double framed side windows are internally sealed to ensure an airtight carcass

UV-absorbing tempered glass sides provide a high level of protection against harmful UV rays when UV lamp is

activated

PERFORMANCE

The cabinet is precisely engineered to deliver the required performance anytime you need it

World first backward-curved motorised impeller system that distributes air within the positive plenum in all

directions to provide lower noise levels, better airflow uniformity, and lower power consumption

Sentinel microprocessor control system with true air flow velocity (both downflow and inflow) sensing

technology. In addition, the airflow sensor itself is able to compensate for change in temperature

Extremely low noise (less than 60dbA) and vibration levels due to proprietary construction and mounting

technology

ISO Class 3 work zone air cleanliness


USER SECTION

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Built-in solid state variable speed controller (infinitely adjustable from zero to the maximum setting) with built-in

RFI and noise filters is superior to conventional step controllers

CLEANABILITY

The issue of cleanability is important to ensure easy and proper decontamination procedures

Smooth and corrosion resistant exterior is free of large protrusions for greater cleanability

Interior surfaces are designed to eliminate protrusions, channels, and any other areas that will be difficult to

clean.

All joints, corners and seams are expertly sealed for maximum cleanability

Work surface trays are fabricated from a single piece of stainless steel without any fasteners, thus eliminating

difficult to clean joints

Generously sized drain pan to contain any liquid spills and allow easier cleaning

OPERATOR COMFORT

The features incorporated in this cabinet allow for greater operator comfort and better working condition Removable, rounded front armrest eliminates pressure points on the arms

Completely frameless UV resistant sliding window for maximum visibility into the work zone.

5000k fluorescent lamp provides 1200 lux of work zone lighting for less glare and greater operator comfort

Electronic ballast for fluorescent lighting resulting in lower heat output, higher energy efficiency, increase

reliability and service life and most importantly zero-flicker

Large 173 mm/6.8 inch work access opening allows easier access into the work zone

Width of the inflow air gril l has been minimized in order to place the work zone closer to the operator

A plugged aerosol sampling port (connected to the common positive plenum) for filter testing by the aerosol

challenge method is accessible from below the work surface tray

EASE OF SERVICE

When it comes to service, we design the cabinet to allow the minimum amount of personnel possible to

perform it while at the same time also keeping the downtime as short as possible

All cabinet electrical system with the exception of the fan motors are located outside contaminated areas and

can be accessed without the need of decontamination

Easy filter access through the front part of the cabinet to minimize downtime

Lifting grip position for filter and blower maintenance on front cover access

Unique hinged front panel

Convenient hand tool kit and extra fasteners is supplied with every cabinet


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COST SAVING

It is possible to achieve a balance between excellent performance and reasonable operating cost.

Therefore, Esco utilizes the following features

1. Automatic standby mode activated when sash is lowered below operating height in order to conserve energy

while maintaining proper airflows in the work zone

2. Permanently lubricated direct drive centrifugal blower energy-efficient external rotor type design

Please refer to chapter 2 of SERVICE SECTION for detailed product specification.

Each individual unit shipped is extensively tested for performance and safety and delivered with a detailed

test report and certificate of performance. Testing performed at our factory laboratory includes:

Airflow velocity (laminar flow, inflow, downflow)

Operator comfort tests: noise, light, vibration

Filter integrity (PAO aerosol challenge)

Containment (KI-Discus and microbiological method) Electrical safety to IEC61010-1

Additional units are also randomly selected on a statistical sampling basis and re-tested using research-

grade instrumentation and additional test protocols at our dedicated Product Development Laboratory.

Esco performs testing in accordance with more than 20 of the worlds most recognized standards, of local,

regional and international scopes. In particular, testing in our laboratory is most frequently conducted based on:

BS EN12469:2000. Biotechnology Performance Criteria for Microbiological Safety Cabinets

NSF49. Class II Biohazard Safety Cabinetry, and

IEST-Recommended Practices.

An NSF-Accredited Biohazard Cabinet Field Certifier is available in-house full-time to supervise all testing

work.


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3. Opening the crate

a. Use a hammer or crowbar to open the top panel carefully. Be aware of exposed nails.

b. Remove the front panel, followed by the two side panels, and finally the back panel.

c. All the retrofit kits are packed separately and placed on top of the cabinet.

These items are listed on the packing checklist pasted on the crate. Please check

the packing checklist carefully to make sure that all items have been included.

Remove them from the top of the cabinet before proceeding further.

If you did not receive one or more retrofit kits lis ted on the packing checklist, or if any of the items are

damaged, please contact your d istributor or Esco Micro Pte Ltd immediately for further inst ructions.

Please refer to section 2.2 for complete listings o f items (aside from the retrofit ki ts) included with your

cabinet.

4. Removing the packaging materiala. The cabinet is protected by styrofoam and shrink wrap.

b. The cabinet is secured with straps to the pallet.

c. Remove the styrofoam and shrink wrap but leave the strap securing the cabinet to the

pallet intact.

d. If you find any damage during this stage of unpacking please refer to Freight Claim Information on

page 6.

e. It is the best practice to leave the cabinet secured with straps to the pallet until the

cabinet is located in its approximate final position to facilitate ease and safety in handling.

Choosing the best location for your cabinet in order to achieve optimum operating performance of

your cabinet is determined by a number of factors. Please refer to the next chapter for some

guidelines.

5. Moving the cabinet

a. When lifting the pallet with the cabinet secured to it, please always ensure that the floor jack or

mechanical lift truck has always entered fully under the pallet. This is to increase the stability of the

cabinet and reduce the risk of the cabinet falling down. Please use extension bar when necessary.

b. During the moving of the cabinet, please ensure there is enough distance between the supports of

pallet and the ground. Dragging the pallet against the ground (at one side or otherwise) will damage

the pallet and possibly your new cabinet.

Chapter II Unpacking Your Cabinet

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6. Removing the strapping

a. Remove the strapping by cutting it at a safe position to prevent any scratch on the surface of your

new cabinet.

Fig. 2 Removing the strapping

b. Do not discard the packaging material for your cabinet until you have checked all of the components,

installed and tested the unit.

7. Lifting the cabinet

a. The cabinet is not designed to support its own weight at the centre portion for extended periods.

However, lifting the cabinet by mechanical lift truck in the centre is allowable. If you must lift the

cabinet manually, use at least 4 persons.

b. Always lift up the cabinet with enough distance in between the fork of the mechanical lift truck and

the centre of the cabinet.

Suggested distance: 50 cm for 3 ft and 90 cm for 4ft and above.

Install the cabinet on the existing work surface or Esco support stand (retrofit kit).

When installing the cabinet onto an existing work surface, ensure that the structure can safely

suppor t the combined weight of the cabinet and any related equipment. Some modifications to

the work surface may be necessary.

The work surface should be smooth and nonporous and resistant to the disinfectants and

chemicals used in conjunct ion with the cabinet.

Instructions on installing the Esco support stand can be found inside the cardboard box with

the support stand

Remove the 2 screws at the back of the cabinet securing the sash before operating the cabinet


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Esco Micro is committed to protecting the environment. Please re-use the pallet if possible, and /or dispose

the packaging materials and crate in a proper manner. Please hammer any exposed nail on the crate surface

prior to disposal.

2.2 PACKAGING CONTENTSThe following items are included together with your manual:

1) Test certificate

2) Test report

In case this manual and/or test report is lost or misplaced, Esco retains a copy in our files. A replacement

copy can be obtained by contacting Esco Micro Pte Ltd and stating the cabinet model, serial number and a

brief description of the information desired.

3) 1 bag containing fasteners replacement

4) 1 M4 screwdriver and 1 M8 spanner for maintenance and service

5) 1 yellow screwdriver for adjusting the speed control

6) 2 biohazard stickers

The following items are permanently attached to the cabinet in a Ziploc bag:

1) Test report

2) Electrical diagram

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CHAPTER III

INSTALLING YOUR CABINET

3.1 CHOOSING A SUITABLE LOCATION

Please follow these guidelines when choosing a suitable location for your cabinet:

1. The location must be far away from :

a. personnel traffic flows

b. air vents (in and out)

c. door and window

d. any other sources of disruptive air currents or air drafts

If the drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access

opening, the potential exists for contaminated air to exit or enter the work zone area of the cabinet. It depends

on the severity of the air current.

2. Exhaust filter area especially susceptible to disruptive air currents or air drafts. Sufficient clearance of 40 cm

(minimum) is recommended between the highest point of the cabinet and the ceiling.

If the distance is less than 40 cm, the airflow alarm system may require re-calibration.

Esco does not guarantee whether this can be done successfully. It has to be verified by the local

distribu tor or service company in the actual place of use.

3. However, for a proper exhaust filter leak scanning purposes, a minimum distance of 50 cm is recommended.

4. A clearance of 183 cm (6ft) in front of cabinet is strongly advised in order to maintain proper airflow.

Fig. 3 Examples of the influence on cabinet airflow from room design and ventilation

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Picture explanation:

No.1 The location of cabinet 1 is appropriate with respect to the avoidance of excessive air movements from the

surrounding.

No.2 Cabinet 2 is too close to the doorway and could be influenced by the air inlet.

No.3 The airflow of cabinet 3 could be influenced by the air inlet.

No.4 Cabinet 4 is too close to the doorway.

No.5 Cabinet 5 is well-sited providing that the adjacent return air grille does not influence cabinet airflow.

The above diagram illustrates the influence on cabinet airflows from room design and ventilation systems.

It is not intended to depict a typical installation. The location of such a number of cabinets in a small room, or in

close proximity, is not recommended.

The following article is the extracts from the British Standard BS 5726- PART 2:1992 on Installation

Recommendation:

I. CABINETS

1.1 The installation of cabinets and their commissioning will normally be the business of the supplier or an

experienced agent. Local engineers may need to be consulted to determine suitable routes to duct

filtered air to the outside.

1.2 For containment level 3 laboratories where there is an installation problem because of difficulties in

exhausting cabinet air to the outside, specialist advice should be obtained.

1.3 Operator protection offered by a cabinet can be adversely affected by poor siting of the unit. It is

important to avoid siting a microbiological safety cabinet in a thoroughfare or in line with a doorway or

openable window. Preliminary airflow visualization tests may help to select the optimum position within a

room. Performance is verified by conducting operator protection tests once the cabinet is in position and

running. In difficult conditions, it may be helpful to make a temporary installation of the cabinet in order to

make these tests before the final installation.

1.4 The siting of safety cabinets in laboratories should be considered at the initial stages of the planning of a

new building or before modification of an existing building. If additional safety cabinets are required in an

existing laboratory, the recommendations given in this clause also apply. The site selected should comply

with the interrelated requirements for containment, extraction, room make-up air, ventilation and general

environmental safety and comfort. The recommendations for siting given here are only intended to act as

a guide to the avoidance of disturbances to the safety cabinet, its operator and other personnel and to theavoidance of interference with escape routes, etc. No specific dimensions or planning arrangements can

prospectively guarantee satisfactory performance of the cabinet in all cases.

1.5 If cabinets are joined together it is strongly recommended that the junctions should be airtight and should

not put undue stress on the cabinets or any junction or adaptor pieces. Sealing gaskets or mating

surfaces should not leave internal cavities or crevices. Note: Attention should be paid to vibration as if it is

excessive damage to seals and gaskets may occur.


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1.6 To avoid disturbances to the safety cabinet and its operator, consideration should be given to the

following.

a) The distance from the plane of the aperture to any circulation space should be at least 1000 mm, so

as to preserve a zone undisturbed by anyone other than the operator see figure 1a.

b) The distance between the front aperture and a bench opposite it should ideally be at least 1500 mm

see figure 1b. However, containment performance is not likely to be affected if this distance is

reduced, for instance to enable an operator to use the bench behind whilst working at the cabinet. In

this case operator movement over a smaller distance may cause less air disturbance. With such

procedures their effect on containment should be measured by relevant operator protections factor

tests.

c) There should be no opposing wall (or other obstruction likely to affect the airflow) within 2000 mm of

the front aperture see figure 1c.

d) Safety cabinets should not be installed in positions where they are likely to be affected by other itemsof equipment. In particular the distance to the aperture of an opposing safety cabinet, fume cupboard,

or the edge of a local exhaust ventilation outlet should be not less than 3000 mm see figure 1d.

e) Any room air supply diffuser should not be within 1500 mm of the front aperture. If there are large

numbers of safety cabinets in a laboratory this recommendation may be difficult to comply with, but

where diffusers have to be placed in close proximity to a safety cabinet, their discharge velocities and

therefore air handling rates will need to be low.

f) A safety cabinet should not be positioned with either side closer than 300 mm from a wall or similar

obstruction see figure 1e.

g) Large obstructions (e.g. and architectural column) projecting beyond the plane of the aperture should

not be within 300 mm of the side of the safety cabinet see figures 1f and g.

h) Doorways should not be within 1500 mm of the aperture or within 1000 mm of the side of the safety

cabinet see figure 1h except where a door includes air transfer grills in which case protection

factor testing should be carried out to ascertain a suitable distance.

1.7 The position of a safety cabinet should satisfy the spatial requirements (e.g. vision, lighting and

convenience of access) of the operator and personnel working nearby. When a cabinet is installed on a

bench top, the leading edge should be flush with or slightly overhanging the edge of the bench top.

Note 1: Typical problems and their solutions are illustrated in figure 2.

Note 2: There should not be an open space between the leading edge of the cabinet and the front of the

bench as this may create turbulence in front of the aperture. It also provides an obstacle which could

adversely affect airflow across the cabinet face


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II. DUCT INSTALLATION

2.1 Cabinet should exhaust air from integral fans by a direct airtight duct less than 2 m in length. If such short

duct connections are not possible the exhaust fan should be situated separate form the cabinet and close

to the discharge end of the duct so that any substantial length of ducting is under negative air pressure.

2.2 The air extraction system should be capable of dealing with external wind pressure up to 250 Pa with

extra capacity of not less than 200 Pa for blockage compensation.

III. CABINET EXTRACTION SYSTEM

3.1 General

The extraction system (together with the arrangements for make-up air) should be so constructed as to

minimize the effect on the safety cabinet of outdoor wind (up to pressures of 250 Pa at the end of the

duct) and other sources of air disturbance. The discharge opening should be sited with due regard to the

flow pattern of air around the building and should be located to minimize the risk of exhaust air beingdrawn back into the building or into neighbouring buildings through open windows or air intakes.

3.2 Cabinet Ductwork

3.2.1 Ductwork should have a smooth, obstruction free interior and should preferably be circular in cross

section without excessive changes in diameter. The extraction ductwork should be clearly marked

with label indicating a biological hazard complying safety sign.

3.2.2 The cross section of the ductwork and the routing of the ducts within the building should be such that

the noise level is within the requirements of the laboratory. However in some cases, e.g. long duct

runs or large volume cabinets, considerations of space may require smaller duct diameters and

consequently give air velocities in excess of this guideline.

3.2.3 Ductwork should follow the most direct route from the cabinet to the discharge point, and it should be

possible to identify the ductwork leading from a particular cabinet. Bends should be kept to a

minimum number and have the largest radii practicable. There should be a minimum of horizontal

run. The ductwork should be deigned to accommodate thermal expansion and contraction. Ductwork

should not violate the fire compartmentation of the building in passing between the safety cabinet and

its final discharge point. If possible, fire dampers should be avoided and fire protection provided by

means of suitable treatment of the ductwork, or by enclosure of the duct within a protected shaft or by

running the ductwork outside the building. If dampers have to be fitted they should be upstream of the

fan.

3.2.4 Whilst no material is completely resistant to all forms of chemical attack, materials of construction for

the ductwork should be chosen to give the best resistance to the chemical and physical conditions to

which they will be subjected, with due consideration to disinfection agents that may be used, and that

the cost and practicability will allow


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3.3 Negative pressure laboratories

In situations where microbiological safety cabinets are installed in negative pressure environments a

pressure differential greater than 40 Pa can develop between the cabinet working space and the

laboratory when the cabinet is turned off. This could result in air flowing out of the safety cabinet aperture

and potentially a risk of formaldehyde leaking out during fumigation. This difficulty can be overcome by

installing a total shut-off damper in the exhaust ducting. A leakage test should be conducted with this

damper closed and the laboratory operating at the designed degree of negative pressure.

IV. MAKE-UP AIR SYSTEMS

4.1 Safety cabinets should be installed only after first considering the details of the make-up air system

necessary to replace all of the air entering the safety cabinets in the laboratory. The safety cabinets and

other air extraction points together with the make-up air supply in the laboratory should be regarded as an

integral system. The air supply system should not compromise the performance of the safety cabinets.4.2 The high air change rate in the laboratory resulting from a multiple safety cabinet installation, or from a

single installation in a small laboratory, necessitates careful selection of the method of supplying make-up

air. Insufficient space may preclude the use of conventional equipment, and special diffusers, grilles, or a

perforated ceiling may be required to achieve low room-air velocities.

4.3 Arrangements for the supply of make-up air should be consistent with the requirements for protection

from fire and smoke and with the achievement of the environmental conditions specified for the

laboratory. Make-up air should be heated or cooled, as necessary, to maintain a laboratory air

temperature within +/- 2oC of that required. The introduction of make-up air should avoid draughts in the

area of safety cabinets.

4.4 For air make-up by passive air inflow, openings, louvers or transfer grilles should be provided in walls and

doors for make-up air to be introduced into the room from its surroundings, preferably from adjacent

heated corridors see figure 3a. The locations and sizes of these openings, louvers or transfer grilles

should be chosen to ensure the avoidance of discomfort due to draughts and to ensure that the opening

and closing of doors does not affect the performance of the safety cabinets. The use of damped door

closure devices can help to reduce sudden air movement. The drawing-in of potentially contaminated air,

e.g. from adjacent laboratories should be avoided and the general quality of the air should be consistent

with the achievement of the environmental conditions specified for the laboratory. Note: Installers and

users should take care not to breach the integrity of the fire protection system particularly when fitting

grilles into doors design to provide half hour fire resistance. Use of intumescent material is often

necessary.


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4.5 The objective of the make-up air distribution system (and any other mechanical ventilation system) should

be to introduce the required volume of air into the room with the minimum possible disruption to the safety

cabinet airflow pattern, and the make-up air supply system should not reduce the degree of protection

afforded to the operator by the safety cabinet. In general, air diffusers, grilles or terminal units (whether

ceiling, wall or floor mounted( should not discharge directly towards or across the safety cabinet aperture.

It is unlikely that the room airflow pattern employed in the performance testing procedure can be realized

in the majority of actual installations. However, every effort should be made to prevent relatively high

velocity air movement (velocity greater than 0.3 m/s) anywhere in the room housing the safety cabinet.

4.6 A fan-assisted source of make-up air should be filtered, heated and otherwise treated as necessary, to

maintain the environmental conditions specified for the laboratory. It is common practice to prevent

pressurization of the laboratory (which would cause a spread of potentially contaminated air into other

areas) by supplying a lower rate of fan-assisted make-up air than the total extraction rate. When there is

a significant reduction in or a complete loss of the air extraction form the room by the safety cabinetinstallation, the fan-assisted inflow of make-up air should be correspondingly reduced, or disconnected,

either automatically or manually following an automatic alarm see figure 3b. At containment level 3 or

above the supply and extraction airflow should be interlocked to prevent positive pressurization of the

room. Note: Consideration should be given to the possibility of reverse flow through a safety cabinet

when the extraction system is low or off and other safety cabinets or containment facilities are on.

Consideration should also be given to situations where several cabinets may be operating independently

in one room.

4.7 If the airflow rate induced by the safety cabinet is inadequate to ventilate the laboratory of if the laboratory

is to be occupied when the safety cabinet is not in use, separate arrangements should be made for room

ventilation. See figures 3c and 3d.

4.8 Air extracted by a separate ventilation system form a room in which a safety cabinet is installed should

not be re-circulated.

V. SITE SURVEY

5.1 As a preliminary to the installation of safety cabinets in a laboratory the site should be surveyed by the

safety cabinet installer to assess environmental conditions and possible exhaust ductwork route(s). The

survey should include a review of the possible positions of the safety cabinet(s) in relation to laboratory

doors and windows, the main routes of foot traffic past the front and the effects of ventilation systems

installed in the laboratory, particularly identifying any draughts or air circulations that are liable to interfere

with cabinet performance, and confirming that there is an adequate air supply to the room.

5.2 Depending on the outcome of the survey, modifications may be necessary to improve the environment

before the contractor can install a safety cabinet so that it complies with performance requirements.


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Chapter III Installing Your Cabinet

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5. Please permit adequate space behind the cabinet for cleaning, maintaining the counterweight system, and for

performing decontamination procedure.

6. A far away position from any source of heat sources (heaters, fan converters, etc.) for optimum operating

conditions. Please refer to the environmental/electrical conditions on the next page for more detailed

information.


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3.2 ENVIRONMENTAL/ELECTRICAL CONDITIONS

This cabinet is designed to be used under the following conditions:

1. Indoor use

2. Altitudes up to 2000 meters or 6600 ft

3. 20% - 60% relative humidity

4. Temperature range from 20 deg C to 30 deg C (68 deg F to 86 deg F).It is recommended that the temperature

in the laboratory be maintained within +/-2 deg C under all conditions

5. Main supply voltage fluctuations not to exceed +/- 10% of the nominal voltage. It is recommended that the

voltage fluctuation doesn't exceed +/- 2% of the nominal voltage at all times

6. Installation Category: II

Installation category (over voltage category) defines the level of transient over voltage which the instrument is

designed to withstand safely. It depends on the nature of the electricity supply and its over voltage protection

means. For example, in CAT II, which is the category used for instruments in installations supplied from a

supply comparable to public mains such as hospital and research laboratories and most industrial laboratories,

the expected transient over voltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply

7. Pollution Degree: 2.0

Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution

degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of

occasional conductivity caused by condensation.

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3.3 INSTALLING YOUR CABINET

1. Please refer to the unpacking your cabinet on the previous chapter section 2.1 step no.7 on lifting the

cabinet

2. Inspect your cabinet carefully, should you find any defect please refer to the Freight Claim Information on

page 6and our Warranty Terms and Conditions on page 8

3. The 2 screws securing the counterweight (behind the cabinet) for shipment should be removed before the

sash can be moved. After this step, move the sash to the normal operating height

4. Wipe down the interior and exterior of the cabinet with water or a mild household detergent

5. Connect cabinet to the main power supply with the supplied power cable. The fan is automatically switched

on. PLS CHECK PANEL message is displayed; please check that all LEDs and buzzer are on for 6 seconds.

Leave the blower on for 3 minutes (warm-up timer the default duration is 3 minutes, however, this may be

adjusted refer to section 4.5.1.2). Each cabinet requires its own dedicated 13A (230V) or 15A (115V)

power outlet which should not be shared with other appliances. For some cabinets with 2 power inlets

(one for the power out lets in the work zone and another for the cabinet blower and electrical systems),

both power inlets should be connected to their own dedicated power supplies.

6. Prior to use, this cabinet should be certified by a qualified certifier. Under normal operating conditions, the

cabinet should be recertified at least annually and when moved or serviced.

For more on info onperformance validation/certification please refer to the next section 3.4

8. The 2 biohazard decal included together with this manual can be pasted on the door leading

to your biohazard laboratory.

WARNING: DO NOT MOVE THE CABINET WITHOUT OBSERVING THE FOLLOWING PRECAUTIONS:

1. The cabinet must be recertified after being moved

2. Observe the necessary precautions when relocating the cabinet as it is heavy

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3.4 PERFORMANCE VALIDATION/CERTIFICATION

After installation and prior to use, cabinet performance must be validated and certified to factory standards.

The following tests should be performed:

Downflow velocity

Inflow velocity

Airflow smoke patternstest

Filter leak test (filter media leak test and filter gasket leak test)

Site installation assessment test

Optional tests:

a. Electrical safety

b. KI Discus for operator protection (Please refer toAppendix C)

c. Light intensity test

d. Vibration level test

e. Noise level test

f. UV intensity test

Airflow alarm calibration should also be performed

The testing methods and equipment required are specified on the test report. It is recommended that these tests be

performed only by a qualified technicianwho is familiar with the methods and procedures for certifying biological

safety cabinets

Esco conducts training courses on the validation and certification of biohazard safety cabinets. If you are

interested you are most welcome to contact your d istribu tor or Esco.

3.4.1 THE IMPORTANCE OF PERFOMANCE VALIDATION/CERTIFICATION

Studies conducted in New York State have estimated that 50,000 to 70,000 workers around the world, die each

year from chronic occupational diseases resulting from past exposures to toxic substances. (New York Committee

for Occupational Safety and Health)

1. A significant percentage of end-users are largely ignorant, uninformed, or relaxed about the safety

specifications and availability of testing.

2. Relying on velocity measurement or simple airflow visualization for deciding safety of cabinets is insufficient.

3. Standardized test procedures and certifications ensure confidence in tests conducted and ensures safety of

the cabinets.

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3.4.2 RECERTIFICATION

After the initial certification, the cabinet shall be recertified at the following situations:

1. Relocation of cabinet

2. Undetermined cause of airflow alarm

3. When the performance is suspected

4. Major maintenance or service (filter changing, blower changing, etc.)

5. After the exhaust damper is adjusted

6. At least annually or 6 months for safety cabinets with more critical applications (for example work with

agents assigned to biosafety level 3)

It is recommended that airflow alarm calibration should also be performed during recertification.

3.4.3 DISCLAIMER

The perfomance and safety of the cabinet, while rigorously evaluated at the factory, cannot be guaranted once after

transit and installation. Therefore the on-site testing is always recommended.

3.4.4 REFERENCES FOR QUALIFIED CERTIFIERS

1. NSF Accredited Class II Biosafety Cabinet Field Certifiers

Please see http://www.nsf.org/Certified/Biosafety-Certifierfor more information

2. I.A.F.C.A member certifying companyPlease see http://www.iafca.com/listview.htmlfor more information

3. Your distributor

4. Esco Micro Pte Ltd

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CHAPTER IV

OPERATING YOUR CABINET

A biological safety cabinet, when used in conjunction with good microbiological techniques, provide an

effective containment system. This chapter aims to provide the user with the basic operation of the cabinet and

recommended working practices.

4.1 SENTINELCONTROL SYSTEM

Fig. 4 Sentinel Keypad User-interface

1. Fan Button

To turn on and turn off the fan

Please kindly refer to section 4.5.3.2for information on setting the fan PIN

2. Light Button

To turn on and turn off the light

Light goes on automatically when sash is at the operating position (READY state)

Light goes off automatically when sash is at SASH ALARM state.

3. Socket Button

To turn on and turn off the electrical socket (retrofit kit)

Electrical outlet can be controlled fully in any sash position. The maximum rating of all the

outlets in the cabinet is 5 A. In case of overloading, the circuit breaker will trip. Disconnect the device

from the outlet and reset the circuit breaker in the electrical control box (inside the front panel) by

pressing its button.

Chapter IV Operating Your Cabinet

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Fig. 5 Screen confirms electrical outlet is on

Fi . 6A Screen confirms electrical outlet is off

Fig. 6B Circuit breaker position

4. UV Button

To turn on and turn off the UV lamp (optional retrofit kit)

UV lamp can only be activated when the sash window is fully closed. Since the sash is

capable of filtering UV rays, users are protected from the harmful UV rays.

UV Timer is provided to control the duration of the decontamination process. Please refer tosection 4.5.1.4 for further information.

UV Hour Meteris provided to monitor the working hours of UV lamp. Please refer to section

4.5.3.5for further information.

Appendix D provides comprehensive information on the usefulness and effectiveness of UV

light against various microbes.

Please refer to the end o f Chapter 1 of SERVICE SECTION for cabinet operation summary.

5. Up and Down Arrow Button

To move upwards and downwards the menu options

To increase and decrease corresponding value inside one of the menu options

To start, stop and reset timer

Handheld stop clock is not allowed to be brought into the working space, as it might be a source

of contamination. For this purpose, TIMER is provided and functions as a stop clock ONLY in READY


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state. It is displayed in HH:MM:SS format. Sash position and airflow velocity is monitored during timer

mode.

User can start by pressing UP arrow button and it will start counting. Pressing UP button again

will stop the timer. User can then press UP button again to resume the timer. During this time, pressing

DOWN button will take the user out of the timer mode, and Timer Reset message is displayed.

Fig. 7 Screen display when timer is running

Fig. 8 Screen display after timer is reset

6. Set or Mute Button

To choose the menu or sub-menu currently displayed on the LCD screen

To proceed to the next step or sequence inside one of the menu options To turn the alarm sound off (only in fully open state during normal mode)

Alarm is disabled when you enter menu options

7. Menu Button

To enter and exit from the menu options

To go back to the previous level of the menu options

To access maintenance mode from ERR.MSWITCH and AIRFAIL! error condition

Some of the menu options allow the user to customize the cabinet for better working

experience. Please kindly refer to section 4.5for detailed explanations for each menu options.

Before operating the cabinet, please ensure that you have set the Admin PIN (0009 by default ) and Fan PIN

(0001) by default. Refer to section 4.5.3.1 and 4.5.3.2 for fur ther instructions.

The Admin PIN has higher priority and can be used to control the fan (override the Fan PIN).

Please contact Esco should you forget your ADMIN PIN.

Concept of Different Sash Window States

1. The sash window should be fully closed when the cabinet is not in use. This helps keep the work zone interior

clean. The sash window also provides a protective barrier in case the UV lamp is used.


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4.2 STARTING THE CABINET

1. Prepare a written checklist of materials/apparatus necessary for immediate usage.

2. Turn off the UV lights (if it is being used) as soon as you enter the work location. Turn on the blower and raise the

sash (default FAN PIN is 0001). Leave the blower on for 3 minutes (warm-up timer the default duration is 3

minutes, however, this may be adjusted - refer to section 4.5.1.2)

3. Wash hands thoroughly using germicidal soap. Wear gloves for hand protection. Gloves should be pulled over the

knitted wrists of the gown rather than worn inside. Double gloving may be necessary for higher risk work.

4. Put on a clean long-sleeves laboratory coat. A solid front, back closing lab gown provides better protection of

personal clothing than the traditional lab coat. A disposable gown should also be considered when higher risk

work is involved.

5. Adjust the seating position so that the user's face is above the front opening. Adjustable stool is recommended.

Please refer to section 4.6 about ergonomics for more info.

6. Open the sash window to its maximum position. Thoroughly surface-decontaminate the work surface, side

glasses, back wall, drain pan, and internal surface of the window using 70% ethanol (or other disinfectant

depending on materials used in the cabinet). Do surface decontamination as well to the UV lights and electrical

outlet when present.

Do not use any disinfectant containing chlorine-based substance as this may cause corrosion of the

stainless steel surfaces.

7. Surface-decontaminate all materials/apparatus before loading them into the work zone. Load and arrange the

materials/apparatus to minimize the movement of contaminated items over clean items by segregating the

contaminated items from the clean items into two equal areas, one on the left, and one on the right to provide

best protection against cross contamination.

8. Put all the material/apparatus approximately 4 inches behind the sash window, whenever possible.

Never use the cabinet to store supplies or laboratory equipment. Placing excessive materials in the

work zone may affect the airflow and containment capabilities of the cabinet


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Do not block the front and back air gril les by any material/apparatus

Fig. 9 Material/apparatus blocking the front and back air grilles

Avoid use of toxic, explosive, or flammable substances within the cabinet unless the use has been

specifically approved by a safety professional

Never operate the cabinet when any alarms are activated

Minimize the use of syringes or other sharp objects within the cabinet

9. After all the material/apparatus is arranged, adjust the sash to the normal operating height and allow the blower to

run for another 3 minutes in order to purge the work zone of contaminants.

10. Minimize room activity (personnel movements, closing and opening of doors, etc.) as these external airflow

disturbances may affect cabinet airflow and containment.

The user is strongly discouraged from using a gas Bunsen burner in the cabinet due to the airflow

disturbance caused by the thermal currents from the burner - which may compromise cabinet performance.

However, when the need for a Bunsen burner is established and approved by the safety officer then it should

be operated at the right side of the work zone.

The airflow sensor on Esco cabinets when installed in the work zone will be mounted on the left side and

operating the burner at the right side will prevent any disruption to the cabinet airflow monitoring system. Please also

refer to the operating instructions and warnings supplied with any gas service fixture retrofit kit supplied by Esco.

Fig. 10 Blackened filters due to carbon residues generated from the usage of Bunsen burner inside the cabinet


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4.3 WORKING IN THE CABINET

1. User should always set the sash to normal working height (READY state) before starting any experiments.

If the alarm indicator light blinks accompanied by the alarm sound, discontinue work immediately and follow

the proper shutdown procedure on the next section 4.4. The user can then refer to the troubleshooting

section (SERVICE SECTION, CHAPTER 3).

If calibration has been performed properly, the LCD will show the downflow velocity (indicated by D) and the

inflow velocity (indicated by I) as it is monitored by the airflow sensor. Sash alarm will be triggered if inflow

velocity falls below its failing point.

2. Keep the front and back air grilles unobstructed by arms or objects.

3. Work from clean to dirty by following the segregation of clean and dirty materials/apparatus as described in the

previous section. Work with one specimen at a time, recap before moving to the next specimens.

4. Work as far into the cabinet as possible, at least 150mm/6 inches back from the front air intake grille. Hold open

tubes and bottles as horizontally as possible to avoid spillage. Discard empty tubes and bottles into the

collection bags inside the cabinet immediately.

5. Use convenient pipetting aids. Do not mouth pipette. Use horizontal pipette discard pans containing appropriate

disinfectant inside the biohazard safety cabinet. Do not use vertical pipette discard canisters on the floor outside

the cabinet. Biohazard collection bags should be placed inside the cabinet.

6. In the case that streaking loops are to be sterilized to prevent cross-contamination of biological materials, an

electrical incinerator is recommended.

7. Surface-decontaminate before removing any potentially contaminated items from the interior.

Never operate your cabinet while the sash alarm indicator is on

Do not change the cabinet original blower speed unless the change is required by a decrease in

measured air velocity. Adjustment should be made only by a qualified technician

Do not operate the cabinet if fan fails to run

8. If you need to remove items from the biohazard cabinet or introduce new items, move in the cabinet in a slow

and controlled manner. Move arms in and out of the work zone opening slowly and in the direction

perpendicular to plane of the work zone opening. The rapid movement of arms in a sweeping motion may


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21 Changi South Street 1 Singapore 486777 PHONE +65 65420

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