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Revision questions 1.2. Ultrastructure of cells
Understandings, Applications and Skills
Understandings:1. Prokaryotes have a simple cell structure without
compartmentalization.2. Eukaryotes have a compartmentalized cell structure.3. Electron microscopes have a much higher resolution than
light microscopes.
Applications and skills:4. Application: Structure and function of organelles within
exocrine gland cells of the pancreas and within palisade mesophyll cells of the leaf.
5. Application: Prokaryotes divide by binary fission.6. Skill: Drawing of the ultrastructure of prokaryotic cells based
on electron micrographs.7. Skill: Drawing of the ultrastructure of eukaryotic cells based
on electron micrographs.8. Skill: Interpretation of electron micrographs to identify
organelles and deduce the function of specialized cells.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
Electron microscopes have a much higher resolution than light microscopes
1. Distinguish between the maximum resolution of the light and the electron microscope
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2. Complete the table below comparing the resolution of the eye with light and electron microscopes:
resolutionMillimetres
(mm)Micrometres
(μm)Nanometres
(nm)Human eye 100,000
Light microscopes
0.0002
Electron microscopes
0.001 1
3. Small pox virus is 200-400 nm long. Explain which of the instruments given in the previous question you would use for its observation.
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Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
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Prokaryotes have a simple cell structure without compartmentalization.Drawing of the ultrastructure of prokaryotic cells based on electron micrographs.
4. Prokaryotes have a simple cell structure.a. Define the term prokaryote.
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b. Draw and label the ultrastructure of a generalized prokaryote and annotate the diagram with the function of each of the labeled parts.
5. This image is a transmission electron micrograph of a bacterium.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
Identify the labelled structures:
I
II
III
IV
6. This is an electron micrograph of the bacterium Escherichia coli.
(a)Draw a diagram of the ultrastructure of the cell, clearly labelling as many structures as you can identify from the micrograph.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
1 μm
http://2007.igem.org/wiki/images/2/2d/Escherichia_coli.jpg
Revision questions 1.2. Ultrastructure of cells
(b) Calculate the magnification of the image.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
Prokaryotes divide by binary fission.
7. http://2007.igem.org/wiki/images/2/2d/Escherichia_coli.jpg Annotate the following diagram with a brief description of the numbered stages.
https://d2gne97vdumgn3.cloudfront.net/api/file/DYa4SXdWQVobZWjkWQtM
Eukaryotes have a compartmentalized cell structure.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
8. Explain the advantages of compartmentalization in eukaryotic cells.
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9. Annotate the following diagram of the eukaryotic cell and write a short outline of the function of each organelle.
Structure and function of organelles within exocrine gland cells of the pancreas and within palisade mesophyll cells of the leaf.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
10. Outline the functions of the following eukaryotic organelles
Organelle Structure (description)
Function of organelle Draw it
Region of the cell containing chromosomes, surrounded by a double membrane, in which there are pores.
Storage and protection of genetic information on chromosomes.
Ribosome
Small spherical subunit consisting of two subunits, some attached to membranes, others free. Spherical organelles surrounded by a single membrane, containing hydrolytic enzymes.
Digestion of structures ad molecules that are not needed in the cell.
Organelles surrounded by two membranes, the inner of which is folded inwards.
Double-membrane containing layers of membranes (thylakoid stacks) and the pigment chlorophyll.
Large, folding membrane structure found close to the nucleus, with ribosomes attached to some surfaces. Large, folded membrane structure found close to the plasma membrane, often with vesicles budding off the outer edge.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
Processes proteins arriving from the rER.
Spherical organelles, single membrane. Contain hydrolytic enzymes
9 x triplets of cylindrical protein fibers
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
11. Label the micrograph below
Deduce whether it’s a plant or animal cell.
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Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
12. Identify as many organelles as you can and deduce the function of the cell.
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13. Identify as many organelles as you can and deduce the function of the cell.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
http://www.doctortee.com/dsu/tiftickjian/cse-img/botany/plant-anat/cell/plant-cell-tem.jpg
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Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]
Revision questions 1.2. Ultrastructure of cells
References
Most material was copied or inspired from
Paine, Chris . “BioKnowledgy DP Notes 1.2 - Ultrastructure of Cells” Web. 18 Jun 2016. <http://www.slideshare.net/diverzippy/bioknowledgy-dp-notes-12?ref=http://www.bioknowledgy.info/12-ultrastructure-of-cells.html>
Taylor, Stephen. "Essential Biology 02.2 & 02.3 Prokaryotes and Eukaryotes.docx." 18 Jun 2016. <https://www.box.net/shared/y1kangxb9t >.
Dr. Miltiadis Kitsos, DP Biology, Platon School, mailto:[email protected]